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Aliases for HCK Gene

Aliases for HCK Gene

  • HCK Proto-Oncogene, Src Family Tyrosine Kinase 2 3
  • Hemopoietic Cell Kinase 2 3 4
  • Hematopoietic Cell Kinase 3 4
  • P59-HCK/P60-HCK 3 4
  • EC 2.7.10.2 4 63
  • P59Hck 3 4
  • P61Hck 3 4
  • EC 2.7.10 63
  • JTK9 3

External Ids for HCK Gene

Previous GeneCards Identifiers for HCK Gene

  • GC20P030428
  • GC20P031338
  • GC20P031385
  • GC20P030103
  • GC20P030641
  • GC20P027428
  • GC20P030645
  • GC20P030648
  • GC20P030652

Summaries for HCK Gene

Entrez Gene Summary for HCK Gene

  • The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]

GeneCards Summary for HCK Gene

HCK (HCK Proto-Oncogene, Src Family Tyrosine Kinase) is a Protein Coding gene. Diseases associated with HCK include adenoid squamous cell carcinoma and cervical adenosquamous carcinoma. Among its related pathways are Immune System and Infectious disease. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is ABL2.

UniProtKB/Swiss-Prot for HCK Gene

  • Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

Gene Wiki entry for HCK Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for HCK Gene

Genomics for HCK Gene

Regulatory Elements for HCK Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for HCK Gene

Chromosome:
20
Start:
32,052,188 bp from pter
End:
32,101,856 bp from pter
Size:
49,669 bases
Orientation:
Plus strand

Genomic View for HCK Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for HCK Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for HCK Gene

Proteins for HCK Gene

  • Protein details for HCK Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P08631-HCK_HUMAN
    Recommended name:
    Tyrosine-protein kinase HCK
    Protein Accession:
    P08631
    Secondary Accessions:
    • A8K1I1
    • B4DQB6
    • E1P5M2
    • Q29RX1
    • Q2VPE2
    • Q504R5
    • Q5T7K1
    • Q5T7K2
    • Q96CC0
    • Q9H5Y5
    • Q9NUA4
    • Q9UMJ5

    Protein attributes for HCK Gene

    Size:
    526 amino acids
    Molecular mass:
    59600 Da
    Quaternary structure:
    • Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with VAV1, WAS and RAPGEF1 (By similarity). Interacts (via SH3 domain) with HIV-1 Nef and Vif. This interaction stimulates its tyrosine-kinase activity. Interacts (via SH3 domain) with HEV ORF3 protein. Interacts with ARRB1 and ARRB2. Interacts with ADAM15. Interacts with FASLG. Interacts with CBL. Interacts with FCGR1A; the interaction may be indirect. Interacts with IL6ST. Interacts (via SH3 domain) with ELMO1. Interacts (via SH3 domain) with TP73. Interacts with YAP1. Interacts with ABL1 and ITGB1, and thereby recruits ABL1 to activated ITGB1. Interacts (via SH3 domain) with C2orf44.
    SequenceCaution:
    • Sequence=AAA52643.1; Type=Frameshift; Positions=20; Evidence={ECO:0000305}; Sequence=BAF82585.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for HCK Gene

    Alternative splice isoforms for HCK Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for HCK Gene

Proteomics data for HCK Gene at MOPED

Post-translational modifications for HCK Gene

  • Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2.
  • Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases.
  • Ubiquitinated by CBL, leading to its degradation via the proteasome.
  • Ubiquitination at Lys 28 and Lys 124
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for HCK (Hck)

Domains & Families for HCK Gene

Gene Families for HCK Gene

Suggested Antigen Peptide Sequences for HCK Gene

Graphical View of Domain Structure for InterPro Entry

P08631

UniProtKB/Swiss-Prot:

HCK_HUMAN :
  • The SH3 domain mediates binding to HIV-1 Nef
  • Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Domain:
  • The SH3 domain mediates binding to HIV-1 Nef
  • Contains 1 protein kinase domain.
  • Contains 1 SH2 domain.
  • Contains 1 SH3 domain.
Family:
  • Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
genes like me logo Genes that share domains with HCK: view

Function for HCK Gene

Molecular function for HCK Gene

GENATLAS Biochemistry:
hemopoietic cell kinase
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
UniProtKB/Swiss-Prot EnzymeRegulation:
Subject to autoinhibition, mediated by intramolecular interactions involving the SH2 and SH3 domains. Kinase activity is also regulated by phosphorylation at regulatory tyrosine residues. Phosphorylation at Tyr-411 is required for optimal activity. Phosphorylation at Tyr-522 inhibits kinase activity. Inhibited by PP1 and A-770041.
UniProtKB/Swiss-Prot Function:
Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
UniProtKB/Swiss-Prot Induction:
Up-regulated during myeloid cell differentiation. The highest levels are detected in fully differentiated phagocytes. Up-regulated by IL2.

Enzyme Numbers (IUBMB) for HCK Gene

Gene Ontology (GO) - Molecular Function for HCK Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity --
GO:0004713 protein tyrosine kinase activity IMP 10092522
GO:0004715 non-membrane spanning protein tyrosine kinase activity IBA --
GO:0005102 receptor binding IBA --
GO:0005515 protein binding IPI 7859737
genes like me logo Genes that share ontologies with HCK: view
genes like me logo Genes that share phenotypes with HCK: view

Animal Models for HCK Gene

MGI Knock Outs for HCK:

Animal Model Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for HCK

In Situ Assay Products

Flow Cytometry Products

No data available for miRNA , Transcription Factor Targets and HOMER Transcription for HCK Gene

Localization for HCK Gene

Subcellular locations from UniProtKB/Swiss-Prot for HCK Gene

Isoform 1: Lysosome. Membrane; Lipid-anchor. Cell projection, podosome membrane; Lipid-anchor. Cytoplasm, cytosol. Note=Associated with specialized secretory lysosomes called azurophil granules. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated.
Isoform 2: Cell membrane; Lipid-anchor. Membrane, caveola; Lipid-anchor. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasmic vesicle. Lysosome. Nucleus. Note=20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions.
Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for HCK Gene COMPARTMENTS Subcellular localization image for HCK gene
Compartment Confidence
cytosol 5
lysosome 5
plasma membrane 5
vacuole 5
cytoskeleton 3
golgi apparatus 3
nucleus 3

Gene Ontology (GO) - Cellular Components for HCK Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005764 lysosome IDA 15998323
GO:0005794 Golgi apparatus IEA --
GO:0005829 cytosol TAS --
GO:0005884 colocalizes_with actin filament IDA 15998323
genes like me logo Genes that share ontologies with HCK: view

Pathways & Interactions for HCK Gene

genes like me logo Genes that share pathways with HCK: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

SIGNOR curated interactions for HCK Gene

Activates:
Inactivates:
Is activated by:
Is inactivated by:
Other effect:

Gene Ontology (GO) - Biological Process for HCK Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002522 leukocyte migration involved in immune response TAS 18538446
GO:0002758 innate immune response-activating signal transduction TAS 18538446
GO:0006468 protein phosphorylation TAS 3496523
GO:0006909 phagocytosis --
GO:0006954 inflammatory response IEA --
genes like me logo Genes that share ontologies with HCK: view

Drugs & Compounds for HCK Gene

(16) Drugs for HCK Gene - From: NovoSeek, HMDB, DrugBank, DGIdb, and FDA Approved Drugs

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
bosutinib Approved Pharma Target, inhibitor Kinase Inhibitors, SRC/BCR-ABL tyrosine kinase inhibitors, Dual Src-Abl inhibitor; antiproliferative 0
Bosulif Approved September 2012 Pharma 0
Adenosine triphosphate Approved Nutra 0
Dasatinib Approved, Investigational Pharma Kinase Inhibitors, SRC/BCR-ABL tyrosine kinase inhibitors 250
imatinib Approved Pharma Kinase Inhibitors, SRC/BCR-ABL tyrosine kinase inhibitors 567

(16) Additional Compounds for HCK Gene - From: NovoSeek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Antagonist, Gating inhibitor 58-64-0
FIBRINOGEN
FMLP
GP 130
H2O2
genes like me logo Genes that share compounds with HCK: view

Transcripts for HCK Gene

Unigene Clusters for HCK Gene

Hemopoietic cell kinase:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for HCK

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for HCK Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15
SP1: - -
SP2: - -
SP3: -
SP4: -

Relevant External Links for HCK Gene

GeneLoc Exon Structure for
HCK
ECgene alternative splicing isoforms for
HCK

Expression for HCK Gene

mRNA expression in normal human tissues for HCK Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for HCK Gene

This gene is overexpressed in Whole Blood (x39.8).

Protein differential expression in normal tissues from HIPED for HCK Gene

This gene is overexpressed in Peripheral blood mononuclear cells (28.1), Monocytes (10.3), and Cervix (7.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for HCK Gene



SOURCE GeneReport for Unigene cluster for HCK Gene Hs.655210

mRNA Expression by UniProt/SwissProt for HCK Gene

P08631-HCK_HUMAN
Tissue specificity: Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.
genes like me logo Genes that share expression patterns with HCK: view

Protein tissue co-expression partners for HCK Gene

- Elite partner

Primer Products

In Situ Assay Products

Orthologs for HCK Gene

This gene was present in the common ancestor of animals.

Orthologs for HCK Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia HCK 35
  • 89.38 (n)
  • 91.6 (a)
HCK 36
  • 92 (a)
OneToOne
dog
(Canis familiaris)
Mammalia HCK 35
  • 89.82 (n)
  • 91.79 (a)
HCK 36
  • 77 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Hck 35
  • 86.7 (n)
  • 90.08 (a)
Hck 16
Hck 36
  • 90 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia HCK 35
  • 97.62 (n)
  • 97.23 (a)
HCK 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Hck 35
  • 86.51 (n)
  • 89.89 (a)
oppossum
(Monodelphis domestica)
Mammalia HCK 36
  • 79 (a)
OneToOne
chicken
(Gallus gallus)
Aves HCK 35
  • 76.1 (n)
  • 78.94 (a)
HCK 36
  • 77 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia HCK 36
  • 78 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia hck 35
  • 65.79 (n)
  • 67.81 (a)
zebrafish
(Danio rerio)
Actinopterygii LOC101885596 35
  • 72.57 (n)
  • 77.26 (a)
hck 36
  • 76 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta Src64B 37
  • 48 (a)
worm
(Caenorhabditis elegans)
Secernentea C24G6.2a 37
  • 35 (a)
C24G6.2b 37
  • 35 (a)
F09A5.2 37
  • 29 (a)
F57B9.8 37
  • 32 (a)
kin-26 37
  • 32 (a)
src-1 37
  • 43 (a)
W01B6.5 37
  • 30 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 50 (a)
ManyToMany
-- 36
  • 54 (a)
ManyToMany
-- 36
  • 51 (a)
ManyToMany
Species with no ortholog for HCK:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for HCK Gene

ENSEMBL:
Gene Tree for HCK (if available)
TreeFam:
Gene Tree for HCK (if available)

Paralogs for HCK Gene

Paralogs for HCK Gene

genes like me logo Genes that share paralogs with HCK: view

Variants for HCK Gene

Sequence variations from dbSNP and Humsavar for HCK Gene

SNP ID Clin Chr 20 pos Sequence Context AA Info Type MAF
rs173355 -- 32,078,334(+) gccac(C/T)gcgcc intron-variant
rs242598 -- 32,076,386(+) AGCTG(C/T)TTCTC intron-variant
rs242599 -- 32,076,571(+) GTGTA(A/G)ACAAC intron-variant
rs242600 -- 32,077,760(+) AACTC(A/G)TGATC intron-variant
rs242601 -- 32,078,031(+) ttttt(G/T)tttgt intron-variant

Structural Variations from Database of Genomic Variants (DGV) for HCK Gene

Variant ID Type Subtype PubMed ID
nsv833953 CNV Loss 17160897
nsv817891 CNV Gain 17921354

Variation tolerance for HCK Gene

Residual Variation Intolerance Score: 80.41% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.92; 48.95% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for HCK Gene

HapMap Linkage Disequilibrium report
HCK
Human Gene Mutation Database (HGMD)
HCK

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for HCK Gene

Disorders for HCK Gene

MalaCards: The human disease database

(5) MalaCards diseases for HCK Gene - From: DISEASES, NovoSeek, and GeneCards

Disorder Aliases PubMed IDs
adenoid squamous cell carcinoma
  • adenoacanthoma
cervical adenosquamous carcinoma
  • adenosquamous cell carcinoma of the cervix uteri
hepatitis e
hiv-1
  • aids, slow progression to
leukemia, chronic myeloid, somatic
  • chronic myeloid leukemia
- elite association
Search HCK in MalaCards View complete list of genes associated with diseases

UniProtKB/Swiss-Prot

HCK_HUMAN
  • Note=Aberrant activation of HCK by HIV-1 protein Nef enhances HIV-1 replication and contributes to HIV-1 pathogenicity.
  • Note=Aberrant activation of HCK, e.g. by the BCR-ABL fusion protein, promotes cancer cell proliferation.

Relevant External Links for HCK

Genetic Association Database (GAD)
HCK
Human Genome Epidemiology (HuGE) Navigator
HCK
Tumor Gene Database (TGDB):
HCK
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
HCK
genes like me logo Genes that share disorders with HCK: view

No data available for Genatlas for HCK Gene

Publications for HCK Gene

  1. c-Abl and Src-family kinases cross-talk in regulation of myeloid cell migration. (PMID: 19903482) Baruzzi A. … Berton G. (FEBS Lett. 2010) 23 67
  2. HIV-1 Nef triggers macrophage fusion in a p61Hck- and protease-dependent manner. (PMID: 20488787) VAcrollet C. … Maridonneau-Parini I. (J. Immunol. 2010) 23 67
  3. Three-dimensional migration of macrophages requires Hck for podosome organization and extracellular matrix proteolysis. (PMID: 19897576) Cougoule C. … Maridonneau-Parini I. (Blood 2010) 23 67
  4. Hematopoietic cell kinase associates with the 40S ribosomal subunit and mediates the ribotoxic stress response to deoxynivalenol in mononuclear phagocytes. (PMID: 20181660) Bae H. … Pestka J.J. (Toxicol. Sci. 2010) 23 67
  5. The possible role of hematopoietic cell kinase in the pathophysiology of COPD. (PMID: 18719065) Yanagisawa S. … Ichinose M. (Chest 2009) 23 67

Products for HCK Gene

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