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HCFC2 Gene

protein-coding   GIFtS: 51

GC12P102962
host cell factor C2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FLJ94012 2
HCF-2 1, 2, 3
HCF2 2, 5
Descriptions
C2 factor 3
host cell factor 2 2
host cell factor C2 2
External Ids
HGNC: 249721
Entrez Gene: 299152
UniProtKB: Q9Y5Z73
Ensembl: ENSG000001117277
Search outside databases for aliases for HCFC2 gene

Previous GC identifer: GC12P102961

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for HCFC2:
This gene encodes one of two proteins which interact with VP16, a herpes simplex virus protein that
initiates virus infection. Both the encoded protein and the original Herpes host cell factor
interact with VP16 through a beta-propeller domain. The original Herpes host cell factor, however,
is effective at initiating viral infection while the encoded protein is not. Transcripts of
varying length due to alternative polyadenylation signals have been described. [provided by
RefSeq]

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the HCFC2 gene  

Entrez Gene cytogenetic band: 12q23.3   Ensembl cytogenetic band:  12q23.3   HGNC cytogenetic band: 12q23.3

HCFC2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P102962:     (about GC identifiers)

Start:
102,982,439 bp from pter
End:
103,021,373 bp from pter
Size:
38,935 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000012.10  NT_019546.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: HCFC2_HUMAN, Q9Y5Z7 (See protein sequence)
Recommended Name: Host cell factor 2  
Size: 792 amino acids; 86779 Da
Subunit: Binds MLL. Component of the MLL complex, at least composed of MLL, ASH2L, RBBP5, DPY30,
WDR5, MEN1, HCFC1 and HCFC2
Subcellular location: Cytoplasm. Nucleus

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_037452.1  

    ENSEMBL proteins: 
    ENSP00000229330 ENSP00000376604 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Host cell factor C2)
    Human Recombinant Proteins from Abnova (HCFC2)
                  OriGene Purified Recombinant Human Protein: HCFC2 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IDA10196288
    GO:0005737 cytoplasm IDA10196288
    GO:0005886 plasma membrane IDA18029348
    GO:0005925 focal adhesion IDA18029348
    About this table

    Antibodies for HCFC2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for HCFC2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Host cell factor C2), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for HCFC2

    Assays for HCFC2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR006652 Kelch_1
     IPR011498 Kelch_2
     IPR003961 FN_III
     IPR015915 Kelch-typ_b-propeller


       GeneDecks  HCFC2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9Y5Z7

    ProtoNet protein and cluster: Q9Y5Z7

    1 Blocks protein family: IPB000515 Binding-protein-dependent transport systems inner membrane component

    UniProtKB/Swiss-Prot: HCFC2_HUMAN, Q9Y5Z7
    Similarity: Contains 3 fibronectin type-III domains
    Similarity: Contains 4 Kelch repeats

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (HCFC2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_013320

                  Applied Biosystems Silencer® siRNAs for HCFC2

                  Sigma-Aldrich siRNA for HCFC2  
                         Sigma-Aldrich shRNA for HCFC2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for HCFC2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_013320
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_013320
                                     untagged cDNA clone in CMV expression vector: NM_013320 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_013320

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003702 RNA polymerase II transcription factor activity TAS10196288
    GO:0003713 transcription coactivator activity TAS10196288
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    1 Interacting protein for HCFC2 (ENSP000002293303) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ASH2LENSP000003408963STRING (score=.792)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006357 regulation of transcription from RNA polymerase II promoter TAS10196288
    GO:0016032 viral reproduction TAS10196288
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for HCFC2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (HCFC2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_013320

                  Sigma-Aldrich siRNA for HCFC2  
                         Sigma-Aldrich shRNA for HCFC2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_013320  

    REFSEQ mRNAs for HCFC2 gene: 

    NM_013320.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_013320  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_013320
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_013320
                                     untagged cDNA clone in CMV expression vector: NM_013320 

    Additional cDNA sequence: 

    AF117210.1 AK223342.1 AK296523.1 AK313466.1 BC033799.1 

    7 DOTS entries:

    DT.213417  DT.409633  DT.100779817  DT.95375371  DT.121164082  DT.91886501  DT.40125488 

    24/91 AceView cDNA sequences (see all 91 ):

    AA382777 NM_013320 AA844108 AA298808 CA450011 AL138197 CB129324 BU689523 
    AF117210 AA421368 AU185964 CB136198 BX484383 AI972100 CA432700 BX116970 
    BC033799 AI670821 AL601403 AA303058 AA600253 CA430675 BM672018 BP369059 

    highest scoring ESTs for HCFC2:

    AA789205 AA278680 AA278688 AA292636 AA292637 AA298808 AA421286 AA600253 AA608906 AA625767 

    Unigene Cluster for HCFC2:

    Host cell factor C2
    Hs.506558  [show with all ESTs]
    Unigene Representative Sequence: NM_013320


    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for HCFC2 (see all 6 )

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b ^ 15 ^ 16 ^ 17a · 17b · 17c · 17d
    SP1:              -                                                                       -     -                                       
    SP2:              -                                               -                       -     -                                       
    SP3:                                                                                      -     -           -                           
    SP4:                                                                                                                                    
    SP5:                                                                                                                                    

    About this scheme

    ECgene alternative splicing isoforms for HCFC2

    2 Ensembl transcripts including schematic representations:
    ENST00000229330  ENST00000392864  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    HCFC2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for HCFC2

    1 / 2 / 3

    7 probe-sets matching HCFC2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    58124_at2, 3 U95-C 1 1.00 1.00 0.82 1.26 AI580890 0.40 1.00 0.76 1

    46110_at2, 3 U95-B 1 1.00 1.00 0.78 1.11 AA789205 1.00 1.00 1.00 1

    58762_r_at2 U95-B 5 1.00 0.95 0.77 0.64 -- -- -- -- --

    219484_at2, 3 U133-A 1 1.00 1.00 -- -- NM_013320 0.60 1.00 0.82 1

    235264_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    219484_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    235264_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  HCFC2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TATGAAGGAC

    SOURCE GeneReport for Unigene cluster: Hs.506558

    UniProtKB/Swiss-Prot: HCFC2_HUMAN, Q9Y5Z7
    Tissue specificity: Highly expressed in testis. Detected at lower levels in spleen, thymus,
    prostate, ovary, small intestine and colon

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for HCFC2 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    HCFC21   -- host cell factor C2 94.18(n)
    95.06(a)
    475449  XM_532674.2  XP_532674.2 
    chimpanzee
    (Pan troglodytes)
    HCFC21   -- host cell factor C2 99.66(n)
    99.37(a)
    452190  XM_509326.2  XP_509326.2 
    cow
    (Bos taurus)
    HCFC21   -- host cell factor C2 94.53(n)
    94.82(a)
    505341  XM_864033.2  XP_869126.2 
    rat
    (Rattus norvegicus)
    Hcfc21   -- host cell factor C2 85.34(n)
    88.77(a)
    314704  NM_001008357.1  NP_001008358.1 
    mouse
    (Mus musculus)
    Hcfc21, 5 105
    host cell factor C21, 5 86.78(n)1
    88.69(a)1
    679331  NM_001081218.11  NP_001074687.11 
     AI5504795  AI8511845  (see all 14)
    About this table        Species with no ortholog for HCFC2

    ENSEMBL Gene Tree for HCFC2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for HCFC2 gene
    HCFC12  KLHDC102  RABEPK2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/225 NCBI SNPs in HCFC2 are shown (see all 225 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 117)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs37651111,2
    A,C,F,H103022499(-) GAAATT/CTGAGG 1 -- ut3112Minor allele frequency- C:0.19EA NA EU WA 2370
    rs7156641,2
    A,C,F,H103023207(+) CTTTCT/CTCTCT 1 -- ut318Minor allele frequency- C:0.32EA NA EU WA 1842
    rs170352061,2
    C,F,H103000443(+) TAAGGG/CGGAAA 1 A/G mis1 trp312Minor allele frequency- C:0.01NA EU EA WA 1050
    rs11858451,2
    C,F,H103004826(-) CACTTC/TATCCC 1 E/K mis15Minor allele frequency- T:0.18MN EU EA WA 600
    rs27310211,2
    H103020968(-) TTATCT/GTGTAT 1 H/Q mis1 ese34Minor allele frequency- G:0.00EU EA WA 416
    rs27238741,2
    H103023655(+) GAGCAA/CATAGC 1 -- ut314Minor allele frequency- C:0.00EU EA WA 418
    rs29308611,2
    H103021026(-) TAATAT/CACATG 1 V/I mis1 ese34Minor allele frequency- C:0.00EU EA WA 406
    rs29564601,2
    H103023709(+) ATGAAG/AATCCC 1 -- ut314Minor allele frequency- A:0.00EU EA WA 420
    rs27310201,2
    H103023743(-) AATATC/GTGCAT 1 -- ut314Minor allele frequency- G:0.00EU EA WA 418
    rs124225011,2
    H102997561(+) ACTTAG/AGTAAG 1 K/E mis14Minor allele frequency- A:0.00EU EA WA 420
    About this table

    HapMap Linkage Disequilibrium images for HCFC2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607926

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/12 PubMed articles for HCFC2 gene (see all 12 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 29915 HGNC: 24972 AceView: HCFC2 Ensembl:ENSG00000111727 euGenes: HUgn29915
    ECgene: HCFC2 H-InvDB: HCFC2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for HCFC2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for HCFC2-related products

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     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for HCFC2 Antibodies for HCFC2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for HCFC2 Browse small molecules at Sigma-Aldrich
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     Recombinant Proteins
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     shRNA in GFP-retroviral vector
     Search Tocris compounds for HCFC2




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     Search Novus for antibodies for HCFC2

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