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HCCA2 Gene

protein-coding GIFtS: 49

GC11M001447

HCCA2 protein
symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
MOB2 ^²

Descriptions
HCCA2 protein ^²
Mob2 homolog ^³

External Ids
Entrez Gene: 81532²
UniProtKB: Q70IA6³
Ensembl: ENSG00000182208⁷

Search outside databases for aliases for HCCA2 gene

Previous GC identifers: GC11M000394 GC11M9E0141 GC11M001453 GC11M001457

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: MOB2_HUMAN, Q70IA6

Function: Stimulates the autophosphorylation and kinase activity of STK38 and STK38L

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the HCCA2 gene

Entrez Gene cytogenetic band: 11p15.5 Ensembl cytogenetic band: 11p15.5

HCCA2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11 GeneLoc Exon Structure

GeneLoc location for GC11M001447: (about GC identifiers)

Start:	1,447,264 bp from pter
End:	1,465,804 bp from pter
Size:	18,541 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000011.8 NT_009237.17

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: MOB2_HUMAN, Q70IA6 (See protein sequence)

Recommended Name: Mps one binder kinase activator-like 2

Size: 237 amino acids; 26927 Da

Subunit: Binds STK38 and STK38L

Subcellular location: Nucleus. Cytoplasm, perinuclear region

Secondary accessions: Q96M67

Alternative splicing: 2 isoforms: Q70IA6-1 Q70IA6-2

Post-translational modifications:

Phosphorylated¹

REFSEQ proteins: NP_443731.2

ENSEMBL proteins:

ENSP00000382600 ENSP00000328694

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (HCCA2)
	Human Recombinant Proteins from Abnova (HCCA2)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IEA	--
GO:0005737	cytoplasm	IEA	--

About this table

Antibodies for HCCA2:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (HCCA2), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (HCCA2)
	Novus Biologicals Antibody for HCCA2

Assays for HCCA2:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q70IA6

ProtoNet protein and cluster: Q70IA6

1 Blocks protein family: IPB005301 Mob1/phocein family

UniProtKB/Swiss-Prot: MOB2_HUMAN, Q70IA6

Similarity: Belongs to the MOB1/phocein family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (HCCA2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_053005

Applied Biosystems Silencer® siRNAs for HCCA2

Sigma-Aldrich siRNA for HCCA2

Sigma-Aldrich shRNA for HCCA2

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for HCCA2
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_053005
Myc/DDK tagged cDNA clone in CMV expression vector: NM_053005
untagged cDNA clone in CMV expression vector: NM_053005

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_053005

UniProtKB/Swiss-Prot: MOB2_HUMAN, Q70IA6

Function: Stimulates the autophosphorylation and kinase activity of STK38 and STK38L

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005515	protein binding	IEA	--
GO:0008270	zinc ion binding	IEA	--
GO:0046872	metal ion binding	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Gene Network Central^TM Interacting Genes and Proteins Network for HCCA2

3 Interacting proteins for HCCA2 (ENSP00000328694³) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
STK38	ENSP00000229812³	STRING (score=.889)
CAB39	ENSP00000258418³	STRING (score=.718)
RAB27A	ENSP00000337761³	STRING (score=.714)

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for HCCA2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (HCCA2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_053005

Sigma-Aldrich siRNA for HCCA2

Sigma-Aldrich shRNA for HCCA2

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_053005

REFSEQ mRNAs for HCCA2 gene:

NM_053005.3

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_053005

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_053005
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_053005
                                 untagged cDNA clone in CMV expression vector: NM_053005

Additional cDNA sequence:

AB066564.1 AJ580639.1 AK057350.1 AK296658.1 AK300235.1 BC035317.1 BC047291.1 BC058934.1

BC067785.1 BX537373.1 CR591411.1 CR592866.1 CR607171.1 CR623644.1

8 DOTS entries:

DT.113456 DT.40192510 DT.97844783 DT.95134020 DT.95134015 DT.75102016 DT.100028270 DT.95134018

24/181 AceView cDNA sequences (see all 181 ):

CR592866 BQ230053 BQ694944 CR623644 AI380671 AI673640 BG055686 BM663806

CD108611 AA670391 CD365072 CR607171 BM015956 AA358209 BU737994 CA946949

CR591411 BM993205 CB140209 AI080064 BC035317 AA806473 BQ883981 BM548188

highest scoring ESTs for HCCA2:

AB066564 AJ580639 AL548794 AW581179 BC035317 BE703233 BE703274 BE703492 BE769891 BE838451

Unigene Cluster for HCCA2:

HCCA2 protein

Hs.370360 [show with all ESTs]

Unigene Representative Sequence: BX537373

GeneLoc Exon Structure

5/7 Alternative Splicing Database (ASD) splice patterns (SP) for HCCA2 (see all 7 )

ExUns:	1a	·	1b	^	2a	·	2b	^	3a	·	3b	·	3c	^	4a	·	4b	·	4c	·	4d	·	4e
SP1:					-				-
SP2:			-		-				-
SP3:			-		-				-
SP4:					-				-
SP5:					-				-

About this scheme

2 Ensembl transcripts including schematic representations:

ENST00000399693 ENST00000329957

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

HCCA2 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for HCCA2

¹ / ² / ³

3 probe-sets matching HCCA2 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
65522_at²^{, 3}	U95-C	1	1.00	1.00	1.00	1.00	W73115	0.80	1.00	0.91	1
223353_at²^{, 3}	U133-B	1	1.00	1.00	--	--	AB028173	0.60	0.69	0.65	1
223353_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: GTGCAGTCCT

SOURCE GeneReport for Unigene cluster: Hs.370360

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for HCCA2 gene from 5/11 species (see all 11 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	LOC611494¹	--	similar to ovary-specific MOB-like protein	87.19(n) 90.39(a)	611494 XM_849165.1 XP_854258.1
chimpanzee (Pan troglodytes)	LOC741421¹	--	similar to HCCA2 protein	98.72(n) 98.72(a)	741421 XM_001152532.1 XP_001152532.1
cow (Bos taurus)	HCCA2¹	--	HCCA2 protein	89.12(n) 92.35(a)	789811 XM_001256452.1 XP_001256453.1
rat (Rattus norvegicus)	Hcca2¹	--	HCCA2 protein	87.21(n) 91.38(a)	499288 XM_574587.2 XP_574587.2
mouse (Mus musculus)	2700078K21Rik^{1, 5}	7⁵	RIKEN cDNA 2700078K21 gene^{1, 5}	87.8(n)¹ 92.77(a)¹	101513¹ NM_028308.2¹ NP_082584.1¹ AF228503⁵ AI256456⁵ (see all 14)

About this table Species with no ortholog for HCCA2

ENSEMBL Gene Tree for HCCA2

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for HCCA2 gene

MOBKL1B² MOBKL1A²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/81 NCBI SNPs in HCCA2 are shown (see all 81 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 83)

AB	SNP ID	Valid	Chr 11 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs9440^1,2	A,C,F,H	1447672(-)	`GTCTCC/TGTGCA`	1	--	ut3¹ ese³		9		MN EA EU WA	2450
	rs4881735^1,2	A,C,F,H	1458977(+)	`CACCAC/AGTCCT`	1	--	ng3¹		4		EU EA WA	420
	rs12786504^1,2	C,F	1446780(+)	`TTCCCG/ACTTCT`	1	--	ng5¹		3		NA	126
--	rs4881725^1,2	A,C	1459330(+)	`TTTTTC/TCTTTC`	1	--	ng3¹ trp³		0	--	--	--	--
	rs11030226^1,2	A	1459214(+)	`GGCGGC/TAGCCT`	1	--	ng3¹		0	--	--	--	--
	rs2878234^1,2	C	1460203(-)	`GCCCCA/GGCCTG`	1	--	ng3¹		0	--	--	--	--
	rs10742185^1,2	C,F,H	1458246(+)	`AACTCC/GGAGAT`	1	S/S	syn¹		4		EU EA WA	418
	rs10767696^1,2	A,C	1458673(+)	`CCATTA/GGGCTT`	1	P/P	syn¹ ese³		0	--	--	--	--
	rs10219175^1,2	F,H	1448155(+)	`TGGACG/ATAGAG`	1	Y/Y	syn¹ ese³		4		EU EA WA	420
	rs7103253^1,2	A,C,F,H	1450338(+)	`CCAACG/AGGCAG`	1	--	int¹		4		EU EA WA	400

About this table

HapMap Linkage Disequilibrium images for HCCA2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 611969

Genetic Association Database: HCCA2

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

8 PubMed articles for HCCA2 gene:

Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. (PubMed id 15067004)^{1, 3, 4} Devroe E.... Silver P.A. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Gene expression profile in Alzheimer's brain screened by molecular indexing. (PubMed id 11223154)³ Doyu M.... Kato K. (2001)
The sequence of the human genome. (PubMed id 11181995)³ Venter J.C....Zhu X. (2001)
[Cloning of liver cancer-related gene HCCA2 and association of that gene with liver cancer] (PubMed id 11798894)⁶ Wang Z....Chen Z. (2001)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )⁴ Aoki H.... Hino O. (2000)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 81532

AceView: HCCA2

Ensembl:ENSG00000182208

euGenes: HUgn81532

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for HCCA2
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for HCCA2:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for HCCA2-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for HCCA2		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for HCCA2		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (HCCA2)
	Antibodies (HCCA2)
	Search for Chimera RNAi
	Custom Service for Mouse Mab
	Custom Service for Rabbit Pab from Full-length Protein

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	Recombinant Proteins (HCCA2)
	Antibodies (HCCA2)

Search OriGene for HCCA2

Search Tocris compounds for HCCA2

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Antibodies for HCCA2

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HCCA2 Gene

protein-coding GIFtS: 49

Aliases &
Descriptions
for HCCA2

Summaries
for HCCA2

Genomic Location
for HCCA2

Proteins
for HCCA2

Protein Domains/
Families
for HCCA2

Gene Function
for HCCA2

Pathways & Interactions
for HCCA2

Drugs & Compounds
for HCCA2

Transcripts
for HCCA2

Expression
for HCCA2

Orthologs
for HCCA2

Paralogs
for HCCA2

SNPs/Variants
for HCCA2

Disorders & Mutations
for HCCA2

Medical News
for HCCA2

Publications
for HCCA2

Search for HCCA2

Genome Databases showing HCCA2

Other Databases showing HCCA2

Specialized Databases showing HCCA2

Licensable Technologies
for HCCA2

Services
for HCCA2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

HCCA2 Gene

protein-coding GIFtS: 49

Aliases & Descriptionsfor HCCA2

Summariesfor HCCA2

Genomic Locationfor HCCA2

Proteinsfor HCCA2

Protein Domains/Familiesfor HCCA2

Gene Functionfor HCCA2

Pathways & Interactionsfor HCCA2

Drugs & Compoundsfor HCCA2

Transcriptsfor HCCA2

Expression for HCCA2

Orthologsfor HCCA2

Paralogsfor HCCA2

SNPs/Variantsfor HCCA2

Disorders & Mutationsfor HCCA2

Medical Newsfor HCCA2

Publicationsfor HCCA2

Search for HCCA2

Genome Databases showing HCCA2

Other Databases showing HCCA2

Specialized Databases showing HCCA2

Licensable Technologiesfor HCCA2

Servicesfor HCCA2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for HCCA2

Summaries
for HCCA2

Genomic Location
for HCCA2

Proteins
for HCCA2

Protein Domains/
Families
for HCCA2

Gene Function
for HCCA2

Pathways & Interactions
for HCCA2

Drugs & Compounds
for HCCA2

Transcripts
for HCCA2

Expression
for HCCA2

Orthologs
for HCCA2

Paralogs
for HCCA2

SNPs/Variants
for HCCA2

Disorders & Mutations
for HCCA2

Medical News
for HCCA2

Publications
for HCCA2

Licensable Technologies
for HCCA2

Services
for HCCA2