HCAR3 Gene
protein-coding GIFtS: 47
GCID: GC12M123200
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hydroxycarboxylic acid receptor 3(Previous name: G protein-coupled receptor 109B ) (Previous symbol: GPR109B)
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Aliases for HCAR3 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
|---|
| Hydroxycarboxylic Acid Receptor 31 2 | | G-Protein Coupled Receptor HM74B2 3 | | GPR109B1 2 3 5 | | PUMAG2 5 | | HCA31 2 3 | | Puma-G1 | | HM741 2 5 | | GTP-Binding Protein2 | | G Protein-Coupled Receptor 109B1 2 | | Hydroxy-Carboxylic Acid Receptor 32 | | Niacin Receptor 22 3 | | Putative Chemokine Receptor2 | | Nicotinic Acid Receptor 22 3 | | HM74B3 | | G-Protein Coupled Receptor 109B2 3 | | NIACR23 | | G-Protein Coupled Receptor HM742 3 | | |
Export aliases for HCAR3 gene to outside databases |
Summaries for HCAR3 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| UniProtKB/Swiss-Prot: HCAR3_HUMAN, P49019Function: Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteractprolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as alow affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are muchhigher than those provided by a normal diet Gene Wiki entry for HCAR3 (Niacin receptor 2)
|
Genomic Views for HCAR3 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000012.11 NC_018923.1 NT_009755.19
Regulatory elements: Search SABiosciences Regulatory transcription factor binding sites for HCAR3 Other transcription factors
Search SABiosciences Chromatin IP Primers for HCAR3
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HCAR3 |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 12q24.31 Ensembl cytogenetic band: 12q24.31 HGNC cytogenetic band: 12q24.31HCAR3 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 12 GeneLoc Exon Structure GeneLoc location for GC12M123200: view genomic region
(about GC identifiers)
Start:
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123,199,303 bp from pter |
End:
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123,201,439 bp from pter |
Size:
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2,137 bases |
Orientation:
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minus strand |
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Proteins for HCAR3 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: HCAR3_HUMAN, P49019 (See
protein sequence)Recommended Name: Hydroxycarboxylic acid receptor 3 Size: 387 amino acids; 44496 Da
Subcellular location: Cell membrane; Multi-pass membrane protein
Secondary accessions: A8K4G5 B2R830 Q8NGE4Explore the universe of human proteins at neXtProt for HCAR3: NX_P49019
HCAR3 Protein expression data from MOPED and PaxDb: --
REFSEQ proteins: NP_006009.2 ENSEMBL proteins: ENSP00000436714 Reactome Protein details: P49019 Human Recombinant Protein Products:
Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table
HCAR3 for ontologies About GeneDecksing
HCAR3 Antibody Products: Assay Products for HCAR3: |
Protein
Domains / Families for HCAR3 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
HCAR3 for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry P49019ProtoNet protein and cluster: P49019 UniProtKB/Swiss-Prot: HCAR3_HUMAN, P49019Similarity: Belongs to the G-protein coupled receptor 1 family |
Function for HCAR3 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Function Summary: UniProtKB/Swiss-Prot: HCAR3_HUMAN, P49019Function: Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteractprolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as alow affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are muchhigher than those provided by a normal diet
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HCAR3 OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HCAR3 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript Custom all cDNA clones Services for HCAR3 | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for HCAR3 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HCAR3  |
In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HCAR3 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004930 | G-protein coupled receptor activity |
TAS | 9205127 | | GO:0045028 | G-protein coupled purinergic nucleotide receptor activity |
IEA | -- |
HCAR3 for ontologies About GeneDecksing
|
Pathways & Interactions for HCAR3 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
| Unified GeneCards pathways  About this table  See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Class A/1 (Rhodopsin-like receptors) | | | 2 | Signaling by GPCR | |
Pathway sources See GeneCards unified pathways Show all pathways
1 BioSystems Pathway for HCAR3 
5/6
Reactome Pathways for HCAR3 (see all 6)
HCAR3 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for HCAR3
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0007186 | G-protein coupled receptor signaling pathway |
TAS | 7505609 |
HCAR3 for ontologies About GeneDecksing
|
Drugs & Compounds for HCAR3 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
HCAR3 for compounds About GeneDecksing
Compounds for HCAR3 available from Tocris Bioscience About this table
| Compound | Action |
CAS
# |
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| Acifran | Hypolipidemic agent; agonist for the GPR109A (HM74A) and GPR109B receptors | [72420-38-3] | | IBC 293 | Selective agonist for the GPR109B (HM74) receptor. Antilipolytic | [306935-41-1] | 1 Novoseek chemical compound relationship for HCAR3 gene About this table
Search CenterWatch for drugs/clinical trials and news about HCAR3 
|
Transcripts for HCAR3 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for HCAR3 gene: NM_006018.2 Unigene Cluster for HCAR3: Hydroxycarboxylic acid receptor 3 Hs.458425 [show with all ESTs]Unigene Representative Sequence: NM_0060181 Ensembl transcript including schematic representation, and UCSC links where relevant: ENST00000528880(uc001ucy.4)
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HCAR3 OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HCAR3 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript Custom all cDNA clones Services for HCAR3 | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for HCAR3 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HCAR3  |
Additional cDNA sequence: AK290930.1 AK313212.1 BC038955.1 BC047891.1 D10923.1 2 DOTS entries: DT.410471 DT.100787462 24/68 AceView cDNA sequences (see all 68): AL545172 N62053 AL546894 NM_177551 AW081241 AW449521 BM008116 CD364466 BM980688 CD364498 CD370595 AL698846 BM971764 AY148884 AL572062 AW264883 BV205728 BE718299 BI820995 CB265428 BC063461 BU684103 BX330698 BX382582 GeneLoc Exon Structure
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Expression for HCAR3 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| HCAR3 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: CTGGACCCCG
About this image Genevestigator expression for HCAR3
SOURCE GeneReport for Unigene cluster: Hs.458425 UniProtKB/Swiss-Prot: HCAR3_HUMAN, P49019Tissue specificity: Expression largely restricted to adipose tissue and spleen SABiosciences Custom PCR Arrays for HCAR3
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HCAR3 Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse / rat HCAR3 | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HCAR3 | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HCAR3 | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HCAR3 |
Orthologs for HCAR3 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for HCAR3 gene from 1/7 species (see all 7) About this table
| Organism |
Taxonomic classification |
Gene |
Description |
Human Similarity |
Orthology Type |
Details |
zebrafish (Danio rerio) |
Actinopterygii |
gpr81-31 |
GPR81-like protein 3 |
52.61(n) 43.2(a) |
  |
100148539 XM_001921202.3 XP_001921237.2 |
ENSEMBL Gene Tree for HCAR3 (if available) TreeFam Gene Tree for HCAR3 (if available)  |
Paralogs for HCAR3 gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| Paralogs for HCAR3 gene
- HCAR12 OXER12 GPR312 HCAR22
17 SIMAP similar genes for HCAR3 using alignment to 2 protein entries: HCAR3_HUMAN (see all proteins):HCAR2 HCAR1 OXER1 GPR31 CMKLR1 LPAR6 P2RY5 LPAR4 CYSLTR1 CYSLTR2 KPG_011 GPR35 GPR17 KPG_007 P2RY1 OXGR1 OPRM1
HCAR3 for paralogs About GeneDecksing
|
Genomic Variants for HCAR3 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 12 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
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HapMap Linkage Disequilibrium report for HCAR3 (123199303 - 123201439 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV) variations for HCAR3: --  | SABiosciences Cancer Mutation PCR Assays |
|
Disorders
/ Diseases for HCAR3 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
HCAR3 for disorders About GeneDecksing
OMIM gene information: 606039
OMIM disorders: --
4 diseases for HCAR3: About MalaCardsdistal hereditary motor neuropathy distal hereditary motor neuropathy, type ii neuropathy schizophrenia Export disorders for HCAR3 gene to outside databases
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Publications for HCAR3 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for HCAR3 gene, integrated from 9 sources (see all 27): (articles sorted by number of sources associating them with HCAR3) | |  | Utopia: connect your pdf to the dynamic world of online information |
- International union of basic and clinical pharmacology. LXXXII: nomenclature and classification of hydroxy-carboxylic acid receptors (GPR81, GPR109A, and GPR109B). (PubMed id 21454438)1, 2, 3 Offermanns S....Ijzerman A.P. (2011)
- Molecular cloning of cDNAs encoding a LD78 receptor and putative leukocyte chemotactic peptide receptors. (PubMed id 7505609)1, 2, 3 Nomura H.... Matsushima K. (1993)
- Molecular identification of high and low affinity receptors for nicotinic acid. (PubMed id 12522134)1, 2, 9 Wise A.... Pike N.B. (2003)
- Deorphanization of GPR109B as a receptor for the beta-oxidation intermediate 3-OH-octanoic acid and its role in the regulation of lipolysis. (PubMed id 19561068)1, 2 Ahmed K.... Offermanns S. (2009)
- Nicotinic acid receptor agonists. (PubMed id 18983141)1, 3 Boatman P.D....Semple G. (2008)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Isolation and chromosomal localization of GPR31, a human gene encoding a putative G protein-coupled receptor. (PubMed id 9205127)1, 3 Zingoni A....Napolitano M. (1997)
- Aromatic D-amino acids act as chemoattractant factors for human leukocytes through a G protein-coupled receptor, GPR109B. (PubMed id 19237584)1, 9 Irukayama-Tomobe Y....Sakurai T. (2009)
- PUMA-G and HM74 are receptors for nicotinic acid and mediate its anti-lipolytic effect. (PubMed id 12563315)1, 9 Tunaru S....Offermanns S. (2003)
- Activated human hydroxy-carboxylic acid receptor-3 sig nals to MAP kinase cascades via the PLC-dependent PKC and MMP-mediated EGFR path ways. (PubMed id 22289163)1 Zhou Q....Zhou N.M. (2012)
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External Searches for HCAR3 gene
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
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Genome Databases showing HCAR3 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing HCAR3 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing HCAR3 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| Name | Description |
| PharmGKB entry for HCAR3 | Pharmacogenomics, SNPs, Pathways | | SeattleSNPs | http://pga.gs.washington.edu/data/gpr109b/ |
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About This Section
| Patent Information for HCAR3 gene: Search GeneIP for patents involving HCAR3
GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products for HCAR3 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
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| | | | Browse OriGene Antibodies | | OriGene shRNA RFP for HCAR3 | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for HCAR3 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HCAR3 | | Browse OriGene Protein Over-expression Lysates | | Browse OriGene Fluorogenic Cell Assay Kits | | OriGene siRNA for HCAR3 | | Browse 3'-UTR reporter clones for miRNA target validation | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HCAR3 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HCAR3 | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | OriGene Custom Antibody Services for HCAR3 | | OriGene Custom Protein Services for HCAR3 | | OriGene Custom Immunoassay Development | | |
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| | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat HCAR3 | | Search QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing HCAR3 | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HCAR3 | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat HCAR3 | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HCAR3 | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HCAR3 |
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| | | Tocris compounds for HCAR3 |
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 | | HCAR3 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HCAR3 |
|  |  |  | | | ThermoFisher Antibodies for HCAR3 |
| | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HCAR3 |
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