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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

HCAR3 Gene

protein-coding   GIFtS: 47
GCID: GC12M123200

hydroxycarboxylic acid receptor 3

(Previous name: G protein-coupled receptor 109B )
(Previous symbol: GPR109B)
 Explore 4 diseases affiliated with
HCAR3 via our new
 Human Malady Compendium 
Biological research products
for HCAR3
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Hydroxycarboxylic Acid Receptor 31 2     G-Protein Coupled Receptor HM74B2 3
GPR109B1 2 3 5     PUMAG2 5
HCA31 2 3     Puma-G1
HM741 2 5     GTP-Binding Protein2
G Protein-Coupled Receptor 109B1 2     Hydroxy-Carboxylic Acid Receptor 32
Niacin Receptor 22 3     Putative Chemokine Receptor2
Nicotinic Acid Receptor 22 3     HM74B3
G-Protein Coupled Receptor 109B2 3     NIACR23
G-Protein Coupled Receptor HM742 3     

External Ids:    HGNC: 168241   Entrez Gene: 88432   Ensembl: ENSG000002553987   OMIM: 6060395   UniProtKB: P490193   

Export aliases for HCAR3 gene to outside databases


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: HCAR3_HUMAN, P49019
Function: Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract
prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a
low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much
higher than those provided by a normal diet

Gene Wiki entry for HCAR3 (Niacin receptor 2)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_009755.19  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for HCAR3
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidHCAR3 promoter sequence
   Search SABiosciences Chromatin IP Primers for HCAR3

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HCAR3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q24.31   Ensembl cytogenetic band:  12q24.31   HGNC cytogenetic band: 12q24.31

HCAR3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
HCAR3 gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M123200:  view genomic region     (about GC identifiers)

Start:
123,199,303 bp from pter      End:
123,201,439 bp from pter
Size:
2,137 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: HCAR3_HUMAN, P49019 (See protein sequence)
Recommended Name: Hydroxycarboxylic acid receptor 3  
Size: 387 amino acids; 44496 Da
Subcellular location: Cell membrane; Multi-pass membrane protein
Secondary accessions: A8K4G5 B2R830 Q8NGE4

Explore the universe of human proteins at neXtProt for HCAR3: NX_P49019

HCAR3 Protein expression data from MOPED and PaxDb: --

REFSEQ proteins: NP_006009.2  
ENSEMBL proteins: 
 ENSP00000436714  
Reactome Protein details: P49019
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Uscn Proteins for HCAR3

Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005886plasma membrane TAS--
GO:0005887integral to plasma membrane TAS7505609
GO:0016021integral to membrane ----


HCAR3 for ontologies           About GeneDecksing



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(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

HCAR3 for domains           About GeneDecksing

3 InterPro domains/families:
 IPR017452 GPCR_Rhodpsn_7TM
 IPR002286 P2_purnocptor
 IPR000276 GPCR_Rhodpsn

Graphical View of Domain Structure for InterPro Entry P49019

ProtoNet protein and cluster: P49019

UniProtKB/Swiss-Prot: HCAR3_HUMAN, P49019
Similarity: Belongs to the G-protein coupled receptor 1 family


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

     UniProtKB/Swiss-Prot: HCAR3_HUMAN, P49019
Function: Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract
prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a
low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much
higher than those provided by a normal diet

miRNA
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hsa-miR-203 hsa-miR-141 hsa-miR-204 hsa-miR-200a hsa-miR-1208 hsa-miR-211
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Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0004930G-protein coupled receptor activity TAS9205127
GO:0045028G-protein coupled purinergic nucleotide receptor activity IEA--


HCAR3 for ontologies           About GeneDecksing



(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways  About this table 
See pathways by source

Super-pathwaycontained gene-specific pathways
1Class A/1 (Rhodopsin-like receptors)
Class A/1 (Rhodopsin-like receptors)1.00
G alpha (i) signalling events0.44
GPCR ligand binding0.75
GPCRs, Class A Rhodopsin-like0.41
2Signaling by GPCR
Signaling by GPCR1.00
Signal Transduction0.56
GPCR downstream signaling0.89

Pathway sources
See GeneCards unified pathways
Show all pathways


1 BioSystems Pathway for HCAR3 
    GPCRs, Class A Rhodopsin-like

5/6        Reactome Pathways for HCAR3 (see all 6)
    GPCR downstream signaling
Signal Transduction
Signaling by GPCR
G alpha (i) signalling events
GPCR ligand binding



HCAR3 for pathways           About GeneDecksing

Interactions:

    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for HCAR3

Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0007186G-protein coupled receptor signaling pathway TAS7505609


HCAR3 for ontologies           About GeneDecksing



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

HCAR3 for compounds           About GeneDecksing

Browse Small Molecules at EMD Millipore
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Compounds for HCAR3 available from Tocris Bioscience    About this table
CompoundAction CAS #
AcifranHypolipidemic agent; agonist for the GPR109A (HM74A) and GPR109B receptors[72420-38-3]
IBC 293Selective agonist for the GPR109B (HM74) receptor. Antilipolytic[306935-41-1]
1 Novoseek chemical compound relationship for HCAR3 gene    About this table
Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
niacin 84.6 19 16386710 (4), 12563315 (3), 15580557 (2), 19237584 (2) (see all 7)

Search CenterWatch for drugs/clinical trials and news about HCAR3 

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
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Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for HCAR3 gene: 
NM_006018.2  

Unigene Cluster for HCAR3:

Hydroxycarboxylic acid receptor 3
Hs.458425  [show with all ESTs]
Unigene Representative Sequence: NM_006018
1 Ensembl transcript including schematic representation, and UCSC links where relevant:
ENST00000528880(uc001ucy.4)

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6 QIAGEN miScript miRNA Assays for microRNAs that regulate HCAR3:
hsa-miR-203 hsa-miR-141 hsa-miR-204 hsa-miR-200a hsa-miR-1208 hsa-miR-211
SwitchGear 3'UTR luciferase reporter plasmidHCAR3 3' UTR sequence
Inhib. RNA
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Additional cDNA sequence: 

AK290930.1 AK313212.1 BC038955.1 BC047891.1 D10923.1 

2 DOTS entries:

DT.410471  DT.100787462 

24/68 AceView cDNA sequences (see all 68):

AL545172 N62053 AL546894 NM_177551 AW081241 AW449521 BM008116 CD364466 
BM980688 CD364498 CD370595 AL698846 BM971764 AY148884 AL572062 AW264883 
BV205728 BE718299 BI820995 CB265428 BC063461 BU684103 BX330698 BX382582 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

HCAR3 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CTGGACCCCG

Microarray
RNAseq (Illumina Body Map)
(100×FPKM)½
SAGE (Serial Analysis of Gene Expression)

About this image
Genevestigator expression for HCAR3

SOURCE GeneReport for Unigene cluster: Hs.458425

UniProtKB/Swiss-Prot: HCAR3_HUMAN, P49019
Tissue specificity: Expression largely restricted to adipose tissue and spleen

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In Situ
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(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of chordates.

Orthologs for HCAR3 gene from 1/7 species (see all 7)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
zebrafish
(Danio rerio)
Actinopterygii gpr81-31 GPR81-like protein 3 52.61(n)
43.2(a)
  100148539  XM_001921202.3  XP_001921237.2 


ENSEMBL Gene Tree for HCAR3 (if available)
TreeFam Gene Tree for HCAR3 (if available) 

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
Paralogs for HCAR3 gene
HCAR12  OXER12  GPR312  HCAR22  
17 SIMAP similar genes for HCAR3 using alignment to 2 protein entries:     HCAR3_HUMAN (see all proteins):
HCAR2    HCAR1    OXER1    GPR31    CMKLR1    LPAR6
P2RY5    LPAR4    CYSLTR1    CYSLTR2    KPG_011    GPR35
GPR17    KPG_007    P2RY1    OXGR1    OPRM1

HCAR3 for paralogs           About GeneDecksing



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

10/122 NCBI SNPs in HCAR3 are shown (see all 122    About this table
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 12 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs557760991,2
--123198832(+) CAAGCA/GGCCGC 1 -- int12Minor allele frequency- G:0.25NA 4
rs22621941,2
C,F,A,--123198921(-) CCTGAA/CGTAAG 1 -- int13Minor allele frequency- C:0.33NA 6
rs22569361,2
C,F,A,--123198936(-) AAGGCA/GGGCAG 1 -- int15Minor allele frequency- G:0.30NA WA 10
rs6312561,2
C,F,A,H,--123199327(-) TACCAC/TTTCAA 1 -- ut313Minor allele frequency- T:0.33NA 6
rs1402888391,2
--123199342(+) TTTGGC/TAAGTA 1 -- ut310--------
rs1503627891,2
--123199392(+) CTCTAA/CCCGTG 1 -- ut310--------
rs1925529221,2
--123199393(+) TCTACC/GCGTGG 1 -- ut310--------
rs29367721,2
--123199394(-) CCCACA/GGGTAG 1 -- ut310--------
rs1495462731,2
--123199395(+) TACCCA/GTGGGG 1 -- ut310--------
rs1137405151,2
C,--123199410(+) GGCACG/ATTTCC 1 -- ut313Minor allele frequency- A:0.36WA EA 240

HapMap Linkage Disequilibrium report for HCAR3 (123199303 - 123201439 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
      Database of Genomic Variants (DGV) variations for HCAR3: --

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

HCAR3 for disorders           About GeneDecksing

OMIM gene information: 606039    OMIM disorders: --

4 diseases for HCAR3:    About MalaCards
distal hereditary motor neuropathy    distal hereditary motor neuropathy, type ii    neuropathy    schizophrenia


Export disorders for HCAR3 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
About This Section

PubMed articles for HCAR3 gene, integrated from 9 sources (see all 27):
(articles sorted by number of sources associating them with HCAR3)
    Utopia: connect your pdf to the dynamic
world of online information

  1. International union of basic and clinical pharmacology. LXXXII: nomenclature and classification of hydroxy-carboxylic acid receptors (GPR81, GPR109A, and GPR109B). (PubMed id 21454438)1, 2, 3 Offermanns S....Ijzerman A.P. (2011)
  2. Molecular cloning of cDNAs encoding a LD78 receptor and putative leukocyte chemotactic peptide receptors. (PubMed id 7505609)1, 2, 3 Nomura H.... Matsushima K. (1993)
  3. Molecular identification of high and low affinity receptors for nicotinic acid. (PubMed id 12522134)1, 2, 9 Wise A.... Pike N.B. (2003)
  4. Deorphanization of GPR109B as a receptor for the beta-oxidation intermediate 3-OH-octanoic acid and its role in the regulation of lipolysis. (PubMed id 19561068)1, 2 Ahmed K.... Offermanns S. (2009)
  5. Nicotinic acid receptor agonists. (PubMed id 18983141)1, 3 Boatman P.D....Semple G. (2008)
  6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
  7. Isolation and chromosomal localization of GPR31, a human gene encoding a putative G protein-coupled receptor. (PubMed id 9205127)1, 3 Zingoni A....Napolitano M. (1997)
  8. Aromatic D-amino acids act as chemoattractant factors for human leukocytes through a G protein-coupled receptor, GPR109B. (PubMed id 19237584)1, 9 Irukayama-Tomobe Y....Sakurai T. (2009)
  9. PUMA-G and HM74 are receptors for nicotinic acid and mediate its anti-lipolytic effect. (PubMed id 12563315)1, 9 Tunaru S....Offermanns S. (2003)
  10. Activated human hydroxy-carboxylic acid receptor-3 sig nals to MAP kinase cascades via the PLC-dependent PKC and MMP-mediated EGFR path ways. (PubMed id 22289163)1 Zhou Q....Zhou N.M. (2012)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 8843 HGNC: 16824 AceView: GPR109A Ensembl:ENSG00000255398 euGenes: HUgn8843
ECgene: HCAR3 H-InvDB: HCAR3

(According to HUGE)
About This Section
  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
NameDescription
PharmGKB entry for HCAR3 Pharmacogenomics, SNPs, Pathways
SeattleSNPshttp://pga.gs.washington.edu/data/gpr109b/

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
About This Section
Patent Information for HCAR3 gene:
Search GeneIP for patents involving HCAR3

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section

 
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GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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VWF
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von Willebrand factor
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