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HAS3 Gene

protein-coding   GIFtS: 56

GC16P067697
hyaluronan synthase 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 2.4.1.212 3
OTTHUMP00000174869 2
Descriptions
HA synthase 3 2, 3
Hyaluronate synthase 3 3
Hyaluronic acid synthase 3 2, 3
hyaluronan synthase 3 2
External Ids
HGNC: 48201
Entrez Gene: 30382
UniProtKB: O002193
Ensembl: ENSG000001030447
Search outside databases for aliases for HAS3 gene

Previous GC identifers: GC16P059825 GC16P069517 GC16P068875 GC16P068916

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for HAS3:
The protein encoded by this gene is involved in the synthesis of the unbranched glycosaminoglycan
hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. This
gene is a member of the NODC/HAS gene family. Compared to the proteins encoded by other members of
this gene family, this protein appears to be more of a regulator of hyaluronan synthesis. Two
transcript variants encoding different isoforms have been found for this gene. [provided by
RefSeq]

UniProtKB/Swiss-Prot: HAS3_HUMAN, O00219
Function: Plays a role in hyaluronan/hyaluronic acid (HA) synthesis

Gene Wiki entry for HAS3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the HAS3 gene  

Entrez Gene cytogenetic band: 16q22.1   Ensembl cytogenetic band:  16q22.1   HGNC cytogenetic band: 16q22.1

HAS3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16P067697:     (about GC identifiers)

Start:
67,697,661 bp from pter
End:
67,710,123 bp from pter
Size:
12,463 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000016.8  NT_010498.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: HAS3_HUMAN, O00219 (See protein sequence)
Recommended Name: Hyaluronan synthase 3  
Size: 553 amino acids; 62998 Da
Cofactor: Magnesium
Subcellular location: Membrane; Multi-pass membrane protein (Probable)
Secondary accessions: A8K5T5 Q9NYP0

REFSEQ proteins (2 alternative transcripts): 
NP_005320.2  NP_619515.1  


ENSEMBL proteins: 
ENSP00000304440 ENSP00000219322 ENSP00000377373 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (HAS3)
Human Recombinant Proteins from Abnova (HAS3)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005887 integral to plasma membrane TAS9169154
GO:0016020 membrane IEA--
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Antibodies for HAS3: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (HAS3), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (HAS3)
Novus Biologicals Antibody for HAS3

Assays for HAS3: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR001173 Glyco_trans_2


   GeneDecks  HAS3 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry O00219

ProtoNet protein and cluster: O00219

1 Blocks protein family: IPB001173 Glycosyl transferase

UniProtKB/Swiss-Prot: HAS3_HUMAN, O00219
Similarity: Belongs to the nodC/HAS family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (HAS3)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_005329

              Applied Biosystems Silencer® siRNAs for HAS3

              Sigma-Aldrich siRNA and siRNA Panels for HAS3  
                     Sigma-Aldrich shRNA for HAS3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for HAS3
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_005329
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_005329
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_005329 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_005329

UniProtKB/Swiss-Prot: HAS3_HUMAN, O00219
Function: Plays a role in hyaluronan/hyaluronic acid (HA) synthesis
Catalytic activity: UDP-alpha-N-acetyl-D-glucosamine +
beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP +
N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent
hyaluronan)
Catalytic activity: UDP-alpha-D-glucuronate +
N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP +
beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent
hyaluronan)
Enzyme Number (IUBMB): EC 2.4.1.212 

Genatlas biochemistry entry for HAS3:
hyaluronan synthase gene 3,63.3kDa

1 MGI mutant phenotype (inferred from 1 allele(MGI details for Has3):

respiratory system

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016757 transferase activity, transferring glycosyl groups IEA--
GO:0050501 hyaluronan synthase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

1 Invitrogen iPath™ Online BioAtlas - Pathway for HAS3 (Maps provided by GeneGo):
 Sphingolipid metabolism

   GeneDecks  HAS3 for the pathways selected above  
About GeneDecksing

UniProtKB/Swiss-Prot: HAS3_HUMAN, O00219
Pathway: Glycan biosynthesis; hyaluronan biosynthesis

 Gene Network CentralTM Interacting Genes and Proteins Network for  HAS3 


3 Interacting proteins for HAS3 (ENSP000003044403) via UniProtKB, MINT, and/or STRING

InteractantInteraction Details
GeneCardExternal ID(s)
CD44ENSP000002783853STRING (score=.75)
HYAL1ENSP000002660313STRING (score=.732)
HYAL2ENSP000003503873STRING (score=.708)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005975 carbohydrate metabolic process TAS9169154
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for HAS3
4 Novoseek chemical compound relationships for HAS3 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
glycosaminoglycan 75.89 2 12757764 (1)
n-acetylglucosamine 28.80 1 14976384 (1)
agarose 11.63 1 10772242 (1)
tyrosine 0.65 1 15905585 (1)
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (HAS3)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_005329

              Sigma-Aldrich siRNA and siRNA Panels for HAS3  
                     Sigma-Aldrich shRNA for HAS3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_005329  NM_138612  

REFSEQ mRNAs for HAS3 gene (2 alternative transcripts): 

NM_005329.2   NM_138612.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_005329  NM_138612  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_005329
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_005329
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_005329 

Additional cDNA sequence: 

AF232772.1 AF234839.1 AK291400.1 BC021853.1 FJ648810.1 FJ648811.1 

6 DOTS entries:

DT.110342  DT.97819483  DT.120681185  DT.99930199  DT.100001166  DT.100822682 

24/64 AceView cDNA sequences (see all 64 ):

NM_005329 BC021853 AA453764 CA487249 CN482851 AA493783 AW157739 BG284710 
AW294351 NM_138612 BX372373 CA439350 BE047664 AI338972 AA227919 BE217826 
AA359665 BX463935 AW071631 BM544036 AA287477 BX385324 AA227578 AW081535 

highest scoring ESTs for HAS3:

AI338972 BC021853 AA194894 AA195575 AA227578 AA227919 AA329123 AA334964 AA359665 AA493783 

Unigene Cluster for HAS3:

Hyaluronan synthase 3
Hs.592069  [show with all ESTs]
Unigene Representative Sequence: NM_005329


GeneLoc Exon Structure

3 Ensembl transcripts including schematic representations:
ENST00000306560  ENST00000219322  ENST00000393782  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

HAS3 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for HAS3

1 / 2 / 3

4 probe-sets matching HAS3 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

54604_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AI338972 0.80 1.00 0.91 1

223541_at2, 3 U133-B 1 1.00 1.00 -- -- AF232772 0.60 1.00 0.82 1

223541_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1552980_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AAGGCTGGAA

SOURCE GeneReport for Unigene cluster: Hs.592069
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for HAS3 gene from 5/9 species (see all 9 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
HAS31   -- hyaluronan synthase 3 92.77(n)
98.73(a)
489739  XM_546859.2  XP_546859.2 
chimpanzee
(Pan troglodytes)
HAS31   -- hyaluronan synthase 3 99.64(n)
100(a)
454195  XM_001168422.1  XP_001168422.1 
cow
(Bos taurus)
HAS31   -- hyaluronan synthase 3 93.13(n)
98.92(a)
286824  XM_616066.3  XP_616066.2 
rat
(Rattus norvegicus)
Has31   -- hyaluronan synthase 3 90.6(n)
97.65(a)
266805  NM_172319.1  NP_758822.1 
mouse
(Mus musculus)
Has31, 5 8 (53.30 cM)5
hyaluronan synthase 31, 5 90.17(n)1
97.11(a)1
151181  NM_008217.31  NP_032243.11 
 AI5954205  AK0285825  (see all 9)
About this table        Species with no ortholog for HAS3

ENSEMBL Gene Tree for HAS3
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for HAS3 gene
HAS12  HAS22  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/49 NCBI SNPs in HAS3 are shown (see all 49 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 27)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 16 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs37599831,2
A,C,F,H67698639(+) TCTGGC/TTGAGG 2 -- ng51 int12Minor allele frequency- T:0.16EA EU 1048
rs37599811,2
A,C,F67698189(+) CTCCCC/TTCCCC 2 -- ng51 int1 trp32Minor allele frequency- T:0.07NA 182
rs22322291,2
C,F,H67701317(+) GGACCG/ATGTGC 2 H/R mis1 ese35Minor allele frequency- A:0.01EU EA WA 540
rs37599821,2
A,C67698248(+) CCCCGC/TTGGGC 2 -- int1 ng510--------
rs80575131,2
F,H67708118(+) TTTGGG/CGAATG 2 -- ut31 int14Minor allele frequency- C:0.08EU EA WA 414
rs22322331,2
H67706674(+) GACATG/AGCCCC 2 -- ut31 int1 ese34Minor allele frequency- A:0.00EU EA WA 418
rs22322321,2
C,F,H,O67706598(+) ATGCTA/GTATCT 2 L/L syn1 int1 ese37Minor allele frequency- G:0.02EU EA WA NA 560
rs22322281,2
A,C,F,H67701078(+) GCCGCA/GTACCA 2 A/A syn19Minor allele frequency- G:0.42EA EU WA NA 2264
rs22322311,2
H67706241(+) CTCCTC/TTTCCT 2 L/L syn1 int1 trp34Minor allele frequency- T:0.00EU EA WA 406
rs37850791,2
A,C,F,H67702977(+) TGTACC/ACACCT 2 -- int18Minor allele frequency- A:0.13EU EA WA 840
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HapMap Linkage Disequilibrium images for HAS3 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 602428

10 Novoseek disease relationships for HAS3 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
colon carcinoma 41.04 7 14566823 (4), 19231585 (1)
mesothelioma 27.11 2 10794710 (1), 15731923 (1)
prostate cancer 23.75 3 11431361 (2), 16816377 (1)
tumors 21.96 8 15763928 (1), 17315194 (1), 19218337 (1), 14566823 (1)
metastasis 19.95 5 19218337 (1), 14566823 (1)
ovarian cancer 15.72 1 15375493 (1)
rheumatoid arthritis 13.37 1 15763928 (1)
primary tumor 1.83 1 14566823 (1)
necrosis 0.00 1 15763928 (1)
cancer 0.00 1 14566823 (1)
About this table

Human Genome Epidemiology Navigator: HAS3 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/70 PubMed articles for HAS3 gene (see all 70 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 3038 HGNC: 4820 AceView: HAS3 Ensembl:ENSG00000103044 euGenes: HUgn3038
ECgene: HAS3 H-InvDB: HAS3
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
GGDBhttp://riodb.ibase.aist.go.jp/rcmg/ggdb/
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for HAS3:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for HAS3-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for HAS3 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for HAS3 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Search Tocris compounds for HAS3




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for HAS3

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