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Aliases & Descriptions for HAS3
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases EC 2.4.1.212 3 OTTHUMP00000174869 2
Descriptions HA synthase 3 2 , 3 Hyaluronate synthase 3 3 Hyaluronic acid synthase 3 2 , 3 hyaluronan synthase 3 2
Search outside databases for aliases for HAS3 genePrevious GC identifers: GC16P059825 GC16P069517 GC16P068875 GC16P068916
Summaries for HAS3 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for HAS3 : The protein encoded by this gene is involved in the synthesis of the unbranched glycosaminoglycanhyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. Thisgene is a member of the NODC/HAS gene family. Compared to the proteins encoded by other members ofthis gene family, this protein appears to be more of a regulator of hyaluronan synthesis. Twotranscript variants encoding different isoforms have been found for this gene. [provided byRefSeq] UniProtKB/Swiss-Prot: HAS3_HUMAN, O00219 Function : Plays a role in hyaluronan/hyaluronic acid (HA) synthesis
Gene Wiki entry for HAS3
Genomic Location for HAS3
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the HAS3 gene Entrez Gene cytogenetic band: 16q22.1 Ensembl cytogenetic band: 16q22.1 HGNC cytogenetic band: 16q22.1 HAS3 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 16 GeneLoc Exon Structure
GeneLoc location for GC16P067697:
(about GC identifiers )
Start:
67,697,661 bp from pter
End:
67,710,123 bp from pter
Size:
12,463 bases
Orientation:
plus strand
RefSeq DNA sequence: NC_000016.8 NT_010498.15 Proteins for HAS3
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: HAS3_HUMAN, O00219 (See
protein sequence )Recommended Name: Hyaluronan synthase 3 Size : 553 amino acids; 62998 Da
Cofactor : Magnesium
Subcellular location : Membrane; Multi-pass membrane protein (Probable)
Secondary accessions : A8K5T5 Q9NYP0
REFSEQ proteins (2 alternative transcripts):
NP_005320.2 NP_619515.1 ENSEMBL proteins: ENSP00000304440 ENSP00000219322 ENSP00000377373 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 2 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for HAS3: Assays for HAS3:
Protein
Domains/ Families for HAS3(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry O00219 ProtoNet protein and cluster: O00219
1 Blocks protein family : IPB001173 Glycosyl transferase UniProtKB/Swiss-Prot: HAS3_HUMAN, O00219 Similarity : Belongs to the nodC/HAS family
Gene Function for HAS3
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
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Clones , and
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SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_005329 Applied Biosystems Silencer ® siRNAs for HAS3 Sigma-Aldrich siRNA and siRNA Panels for HAS3 Sigma-Aldrich shRNA for HAS3 Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_005329                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_005329                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_005329  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_005329 UniProtKB/Swiss-Prot: HAS3_HUMAN, O00219 Function : Plays a role in hyaluronan/hyaluronic acid (HA) synthesisCatalytic activity : UDP-alpha-N-acetyl-D-glucosamine +beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP +N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascenthyaluronan) Catalytic activity : UDP-alpha-D-glucuronate +N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP +beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascenthyaluronan) Enzyme Number (IUBMB): EC 2.4.1.212
Genatlas biochemistry entry for HAS3 :hyaluronan synthase gene 3,63.3kDa
1 MGI mutant phenotype (inferred from 1 allele ) (MGI details for Has3) :2 Gene Ontology (GO) molecular function terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0016757 transferase activity, transferring glycosyl groups
IEA -- GO:0050501 hyaluronan synthase activity
IEA --
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Pathways & Interactions for HAS3
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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UniProtKB/Swiss-Prot: HAS3_HUMAN, O00219 Pathway : Glycan biosynthesis; hyaluronan biosynthesis
Gene Network CentralTM Interacting Genes and Proteins Network for HAS3 3 Interacting proteins for HAS3 (ENSP00000304440 3 ) via UniProtKB, MINT, and/or STRING About this table 1 Gene Ontology (GO) biological process term (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0005975 carbohydrate metabolic process
TAS 9169154
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Drugs & Compounds for HAS3 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for HAS3 4 Novoseek chemical compound relationships for HAS3 gene
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Transcripts for HAS3(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_005329 Sigma-Aldrich siRNA and siRNA Panels for HAS3 Sigma-Aldrich shRNA for HAS3 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_005329 NM_138612
REFSEQ mRNAs for HAS3 gene (2 alternative transcripts): NM_005329.2 NM_138612.1
Applied Biosystems TaqMan ® Gene Expression Assays: NM_005329 NM_138612
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_005329                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_005329                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_005329  
Additional cDNA sequence: AF232772.1 AF234839.1 AK291400.1 BC021853.1 FJ648810.1 FJ648811.1
6 DOTS entries : DT.110342 DT.97819483 DT.120681185 DT.99930199 DT.100001166 DT.100822682
24/64 AceView cDNA sequences (see all 64
):NM_005329 BC021853 AA453764 CA487249 CN482851 AA493783 AW157739 BG284710 AW294351 NM_138612 BX372373 CA439350 BE047664 AI338972 AA227919 BE217826 AA359665 BX463935 AW071631 BM544036 AA287477 BX385324 AA227578 AW081535
highest scoring ESTs for HAS3 :AI338972 BC021853 AA194894 AA195575 AA227578 AA227919 AA329123 AA334964 AA359665 AA493783
Unigene Cluster for HAS3: Hyaluronan synthase 3 Hs.592069 [show with all ESTs ] Unigene Representative Sequence: NM_005329 GeneLoc Exon Structure 3 Ensembl transcripts including schematic representations : ENST00000306560
ENST00000219322
ENST00000393782
Expression for HAS3
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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HAS3 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for HAS3 1 / 2 / 3
4 probe-sets matching HAS3 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: AAGGCTGGAASOURCE GeneReport for Unigene cluster: Hs.592069
Orthologs for HAS3
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for HAS3 gene from 5/9 species (see all 9
)
About this table Species with no ortholog for HAS3 ENSEMBL Gene Tree for HAS3 Paralogs for HAS3 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for HAS3 gene HAS1 2 HAS2 2
SNPs/Variants for HAS3 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for HAS3 (up to first 250kb)
Disorders & Mutations for HAS3
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
Novoseek , PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 602428 10 Novoseek disease relationships for HAS3 gene
About this table Human Genome Epidemiology Navigator: HAS3 (1 document)
Medical News for HAS3 (Possibly Related Articles in
Doctor's Guide )
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--
Publications for HAS3 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/70 PubMed articles for HAS3 gene (see all 70
): Molecular cloning and characterization of a cDNA encoding the third putative mammalian hyaluronan synthase. (PubMed id 9083017) 1, 2, 3, 4 Spicer A.P....McDonald J.A. (1997) Chromosomal localization of the human and mouse hyaluronan synthase genes. (PubMed id 9169154) 1, 2, 3 Spicer A.P....McDonald J.A. (1997) Hyaluronan synthase-3 is upregulated in metastatic colon carcinoma cells and manipulation of expression alters matrix retention and cellular growth. (PubMed id 14566823) 1, 3 Bullard K.M....McCarthy J.B. (2003) Hyaluronan synthase 3 overexpression promotes the growth of TSU prostate cancer cells. (PubMed id 11431361) 1, 3 Liu N.... Zhang L. (2001) Hyaluronan synthase 3 regulates hyaluronan synthesis in cultured human keratinocytes. (PubMed id 11851874) 1, 3 Sayo T....Inoue S. (2002) Expression of human hyaluronan synthases in response to external stimuli. (PubMed id 10794710) 1, 3 Jacobson A....Heldin P. (2000) Further evidence for the association of MMP9 with nephropathy in type 2 diabetes and application of DNA pooling technology to candidate gene screening. (PubMed id 18587729) 1, 3 Nair S....Bowen T. (2008) Manipulation of hyaluronan synthase expression in prostate adenocarcinoma cells alters pericellular matrix retention and adhesion to bone marrow endothelial cells. (PubMed id 11790779) 1, 3 Simpson M.A....McCarthy J.B. (2002) Up-regulation of putative hyaluronan synthase mRNA by basic fibroblast growth factor and insulin-like growth factor-1 in human skin fibroblasts. (PubMed id 11378333) 1, 3 Kuroda K....Shinkai H. (2001) Inhibition of hyaluronan synthases decreases matrix metalloproteinase-7 (MMP-7) expression and activity. (PubMed id 19231585) 1, 3 Dunn K.M....McCarthy J.B. (2009)
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Genome Databases showing HAS3
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing HAS3
(According to HUGE )
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--
Specialized Databases showing HAS3 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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Name Description
GGDB http://riodb.ibase.aist.go.jp/rcmg/ggdb/
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-- Services for HAS3 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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Products for HAS3:
Recombinant Proteins (HAS3 ) Antibodies (HAS3 )
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