Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 
or upload a file of gene symbols


Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

HARS Gene

protein-coding   GIFtS: 70
GCID: GC05M140034

histidyl-tRNA synthetase
 Explore 20 diseases affiliated with
HARS via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for HARS    

Aliases
for HARS gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
Histidyl-TRNA Synthetase1 2 3     USH3B2
HRS2 3     Histidine Translase2
HisRS2 3     Histidine--TRNA Ligase, Cytoplasmic2
EC 6.1.1.213 8     

External Ids:    HGNC: 48161   Entrez Gene: 30352   Ensembl: ENSG000001704457   OMIM: 1428105   UniProtKB: P120813   

Export aliases for HARS gene to outside databases

Previous GC identifers: GC05M139869 GC05M140636 GC05M140036 GC05M140081 GC05M135198


Summaries
for HARS gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
Entrez Gene summary for HARS:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded
by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is
responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into
proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes
share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune
disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this
gene. (provided by RefSeq, Apr 2012)

Gene Wiki entry for HARS


Genomic Views
for HARS gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000005.9  NC_018916.1  NT_029289.11  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the HARS gene promoter:
         SRF   AREB6   PPAR-gamma1   AML1a   SRF (504 AA)   ARP-1   Nkx2-5   PPAR-gamma2   NF-E2 p45   YY1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: HARS promoter sequence
   Search SABiosciences Chromatin IP Primers for HARS

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HARS


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q31.3   Ensembl cytogenetic band:  5q31.3   HGNC cytogenetic band: 5q31.3

HARS Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
HARS gene location

GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M140034:  view genomic region     (about GC identifiers)

Start:
 140,052,758 bp from pter      End:
 140,071,609 bp from pter
Size:
 18,852 bases      Orientation:
 minus strand

Proteins
for HARS gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: SYHC_HUMAN, P12081 (See protein sequence)
Recommended Name: Histidine--tRNA ligase, cytoplasmic  
Size: 509 amino acids; 57411 Da
Subcellular location: Cytoplasm
Sequence caution: Sequence=CAA28956.1; Type=Frameshift; Positions=Several;
3 PDB 3D structures from and Proteopedia for HARS:
1X59 (3D)        4G84 (3D)        4G85 (3D)    
Secondary accessions: B4DY73
Alternative splicing: 2 isoforms:  P12081-1   P12081-2   

Explore the universe of human proteins at neXtProt for HARS: NX_P12081

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_P12081

    • 4/18 DME Specific Peptides for HARS (P12081) (see all 18)
     LKTPKGT  GLDYYTG  CFKRHGA  GGRYDGL 

    HARS Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (4 alternative transcripts): 
    NP_001244969.1  NP_001244970.1  NP_001244971.1  NP_002100.2  

    ENSEMBL proteins: 
     ENSP00000425634   ENSP00000387893   ENSP00000393244   ENSP00000430063   ENSP00000304668  
     ENSP00000411511   ENSP00000411085   ENSP00000425889   ENSP00000421576   ENSP00000413605  
    Reactome Protein details: P12081
    Human Recombinant Protein Products: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate: HARS
    OriGene Custom Protein Services for HARS 
    GenScript Custom Purified and Recombinant Proteins Services for HARS
    Novus Biologicals HARS Proteins
    Novus Biologicals HARS Lysate
    Browse Sino Biological Recombinant Proteins
    ProSpec Recombinant Protein for HARS
    Uscn Proteins for HARS

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm NAS--
    GO:0005829cytosol TAS--


    HARS for ontologies           About GeneDecksing



    HARS Antibody Products: 
    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    Browse OriGene Antibodies
    OriGene Custom Antibody Services for HARS 
    GenScript Custom Superior Antibodies Services for HARS
    Novus Biologicals HARS Antibodies
    Abcam antibodies for HARS 
    Uscn Antibodies for HARS
    ThermoFisher Antibodies for HARS

    Assay Products for HARS: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Immunoassay Development
    Browse OriGene Fluorogenic Cell Assay Kits
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for HARS
    Browse Enzo Life Sciences for kits & assays
    Uscn ELISAs and CLIAs for HARS

    Protein Domains /
    Families
    for HARS gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HARS for domains           About GeneDecksing

    5/7 InterPro domains/families (see all 7):
     IPR002314 aa-tRNA-synt_IIb_cons-dom
     IPR000738 WHEP-TRS
     IPR006195 aa-tRNA-synth_II
     IPR015807 His-tRNA-ligase
     IPR004154 Anticodon-bd

    Graphical View of Domain Structure for InterPro Entry P12081

    ProtoNet protein and cluster: P12081

    2 Blocks protein families:
    IPB000738 WHEP-TRS domain
    IPB004154 Anticodon binding domain


    UniProtKB/Swiss-Prot: SYHC_HUMAN, P12081
    Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family
    Similarity: Contains 1 WHEP-TRS domain


    Function
    for HARS gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: SYHC_HUMAN, P12081
    Catalytic activity: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

         Genatlas biochemistry entry for HARS:
    histidyl-tRNA synthetase,target autoantibodies in the immune disease polymyositis/dermatomyositis

    Enzyme Number (IUBMB): EC 6.1.1.211 2

    miRNA
    Products:            		miRTarBase miRNAs that target HARS:
    hsa-mir-16 (MIRT001460)

    OriGene 3'-UTR Clone: HARS
    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat HARS
    5 QIAGEN miScript miRNA Assays for microRNAs that regulate HARS:
    hsa-miR-19b hsa-miR-129-5p hsa-miR-518a-5p hsa-miR-26b* hsa-miR-19a
    SwitchGear 3'UTR luciferase reporter plasmid: HARS 3' UTR sequence
    Inhib. RNA
    Products:            		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for HARS (see all 7)
    OriGene shRNA RFP: HARS
    OriGene siRNA: HARS
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat HARS

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for HARS

    Clone
    Products:             		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HARS (see all 3)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HARS
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector: HARS (NM_002109)
    Sino Biological Human cDNA Clone for HARS
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for HARS
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HARS 

    Cell Line
    Products:             		GenScript Custom overexpressing Cell Line Services for HARS
    Search LifeMap BioReagents cell lines for HARS

    In Situ Assay
    Products:       
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HARS

    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004812aminoacyl-tRNA ligase activity ----
    GO:0004821histidine-tRNA ligase activity NAS1549469
    GO:0005524ATP binding NAS--


    HARS for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for HARS:
     Cell cycle / mitosis defect 


    Pathways & Interactions
    for HARS gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1tRNA Aminoacylation
    		tRNA Aminoacylation1.00
    		Aminoacyl-tRNA biosynthesis0.54
    		Cytosolic tRNA aminoacylation0.57
    2Generic Transcription Pathway
    		Gene Expression0.46

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    3        Reactome Pathways for HARS
        Cytosolic tRNA aminoacylation
    tRNA Aminoacylation
    Gene Expression


    1         Kegg Pathway  (Kegg details for HARS):
        Aminoacyl-tRNA biosynthesis


    HARS for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for HARS

    STRING Interaction Network Preview (showing 5 interactants - click image to see 18)

    5/117 Interacting proteins for HARS (P120812, 3 ENSP000003046684) via UniProtKB, MINT, STRING, and/or I2D (see all 117)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RPL5P467772, 3, ENSP000003593454MINT-6542674 I2D: score=2 STRING: ENSP00000359345
    UBCP0CG482, 3, ENSP000003448184MINT-6542674 I2D: score=2 STRING: ENSP00000344818
    TARSP266392, 3, ENSP000002651124MINT-6542674 I2D: score=2 STRING: ENSP00000265112
    TNIKQ9UKE53, ENSP000003995114I2D: score=2 STRING: ENSP00000399511
    THG1LQ9NWX63, ENSP000002311984I2D: score=1 STRING: ENSP00000231198
    About this table

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006412translation NAS7755634
    GO:0006418tRNA aminoacylation for protein translation TAS--
    GO:0006427histidyl-tRNA aminoacylation IEA--
    GO:0010467gene expression TAS--


    HARS for ontologies           About GeneDecksing



    Drugs & Compounds
    for HARS gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for HARS

    5 HMDB Compounds for HARS    About this table
    CompoundSynonyms CAS #PubMed Ids
    Adenosine monophosphate5'-AMP (see all 28)61-19-8--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    L-Histidine(S)-1H-Imidazole-4-alanine (see all 20)71-00-1--
    Phosphoric acidacide phosphorique (FRENCH) (see all 20)7664-38-2--
    Pyrophosphate(4-)Diphosphoric acid ion (see all 10)14000-31-8--

    1 DrugBank Compound for HARS    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    L-Histidine(S)-4-(2-Amino-2-carboxyethyl)imidazole (see all 7)71-00-1target--17150756 16741232

    Search CenterWatch for drugs/clinical trials and news about HARS / SYHC 

    Transcripts
    for HARS gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for HARS gene (4 alternative transcripts): 
    NM_001258040.1  NM_001258041.1  NM_001258042.1  NM_002109.4  

    Unigene Cluster for HARS:

    Histidyl-tRNA synthetase
    Hs.528050  [show with all ESTs]
    Unigene Representative Sequence: NM_002109
    14 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000504156(uc003lgv.3 uc011czm.2 uc003lgw.3 uc011czn.2 uc011czo.2 uc011czq.2)
    ENST00000457527 ENST00000431330(uc011czp.2) ENST00000504366(uc003lgu.3)
    ENST00000307633 ENST00000438307 ENST00000509087 ENST00000415192 ENST00000506579
    ENST00000507746 ENST00000512396 ENST00000518126 ENST00000502888 ENST00000448240


    miRNA
    Products:             		 OriGene 3'-UTR Clone: HARS
    Browse OriGene MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat HARS
    5 QIAGEN miScript miRNA Assays for microRNAs that regulate HARS:
    hsa-miR-19b hsa-miR-129-5p hsa-miR-518a-5p hsa-miR-26b* hsa-miR-19a
    SwitchGear 3'UTR luciferase reporter plasmid: HARS 3' UTR sequence
    Inhib. RNA
    Products:             		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for HARS (see all 7)
    OriGene shRNA RFP: HARS
    OriGene siRNA: HARS
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat HARS
    Clone
    Products:             		 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HARS (see all 3)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HARS
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector: HARS (NM_002109)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for HARS
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HARS 
    Primer
    Products:            		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HARS
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat HARS
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HARS
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HARS

    Additional cDNA sequence: 

    AK000498.1 AK094406.1 AK225776.1 AK293154.1 AK293531.1 AK295219.1 AK302295.1 AK303778.1 
    AK307669.1 AY995220.1 BC011807.2 BC080514.1 

    24/25 DOTS entries (see all 25):

    DT.452906  DT.97864368  DT.100813105  DT.95171401  DT.100783763  DT.91764618  DT.435033  DT.101966816 
    DT.100030876  DT.120887671  DT.120887591  DT.120887651  DT.120887685  DT.100030880  DT.120887619  DT.120887647 
    DT.120887550  DT.91719427  DT.95171393  DT.100769780  DT.100814557  DT.115301  DT.95120098  DT.95171385 

    24/386 AceView cDNA sequences (see all 386):

    BU538121 BC080514 BQ958600 BQ878943 AI583119 BQ428604 AU122521 CA425270 
    CR609422 CB148142 AW247134 BP373147 AA375856 BE464395 BE278945 CR595110 
    BU166382 BM825920 NM_002109 BE293680 BI488738 AA984229 BG177786 BQ961534 

    GeneLoc Exon Structure

    5/12 Alternative Splicing Database (ASD) splice patterns (SP) for HARS (see all 12)    About this scheme

    ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b · 5c · 5d ^ 6a · 6b · 6c · 6d ^ 7 ^ 8a · 8b · 8c ^ 9 ^ 10 ^ 11a ·
    SP1:                                                  -           -                 -     -                                                                 -   
    SP2:                                                  -     -     -     -     -     -     -                                                                     
    SP3:                                                  -           -           -     -     -                                                                     
    SP4:                                            -     -           -                 -     -                                                                     
    SP5:                                                  -     -     -     -     -     -     -     -     -     -     -                                             

    ExUns: 11b · 11c ^ 12 ^ 13a · 13b
    SP1:  -                           
    SP2:                              
    SP3:                              
    SP4:                              
    SP5:                              


    ECgene alternative splicing isoforms for HARS

    Expression
    for HARS gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    HARS expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TGTGGGGCTC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    HARS expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    2 LifeMap In Vivo Development Anatomical Compartments/Cells 
    Tissue Anatomical Compartment CellCategory (developmental path)
    BrainMedulla OblongataBrain
    HeartRight VentricleHeart
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See HARS Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for HARS

    SOURCE GeneReport for Unigene cluster: Hs.528050

    UniProtKB/Swiss-Prot: SYHC_HUMAN, P12081
    Tissue specificity: Brain, heart, liver and kidney

        SABiosciences Custom PCR Arrays for HARS
    Primer
    Products:    		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HARS
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat HARS
    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HARS
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HARS
    In Situ
    Assay Products:     
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HARS

    Orthologs
    for HARS gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the last universal common ancestor (LUCA).

    Orthologs for HARS gene from 10/43 species (see all 43)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    chicken
    (Gallus gallus)    		 Aves HARS1 histidyl-tRNA synthetase 77.15(n)
    83.77(a)    		   416132  NM_001006144.1  NP_001006144.1 
    lizard
    (Anolis carolinensis)    		 Reptilia --
    		 --
    		 81(a)
    		 1 → many
    		 4(77715768-77743628)
    African clawed frog
    (Xenopus laevis)    		 Amphibia BU904843.12   -- 73.61(n)    BU904843.1 
    zebrafish
    (Danio rerio)    		 Actinopterygii Dr.41612 Transcribed sequence with weak similarity to protein spP12081 (H.sapiens) SYH_HUMAN Histidyl-tRNA synthetase (Histidine--tRNA ligase) (HisRS) less 75.81(n)    57054842 
    fruit fly
    (Drosophila melanogaster)    		 Insecta CG63353
    Aats-his1    		 histidine-tRNA ligase3
    Histidyl-tRNA synthetase1    		 63(a)3
    62.58(n)1
    65.66(a)1    		   17C13
    328411  NM_133077.11  NP_573305.11 
    worm
    (Caenorhabditis elegans)    		 Secernentea hrs-13
    hars-11    		 histidyl-tRNA synthetase (cytoplasmic)3
    Protein HARS-11    		 53(a)3
    57.16(n)1
    56.21(a)1    		   IV(10859154-10861249)3
    1779781  NM_001028202.31  NP_001023373.11 
    baker's yeast
    (Saccharomyces cerevisiae)    		 Saccharomycetes HTS1(YPR033C)4
    HTS11    		 Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome less4
    Hts1p1    		 53.35(n)1
    53.35(a)1    		   16(639019-637379)4
    8561451, 4  NP_015358.11, 4 
    thale cress
    (Arabidopsis thaliana)    		 eudicotyledons AT3G027601 histidyl-tRNA synthetase 57.08(n)
    56.62(a)    		   820930  NM_111144.5  NP_186925.4 
    rice
    (Oryza sativa)    		 Liliopsida AK099789.12   -- 78.04(n)    AK099789.1 
    E. coli
    (Escherichia coli)    		 Gamma proteobacteria hisS6
    		 histidyl tRNA synthetase
    		 20(a)
    		 possible ortholog
    		 Chromosome(2637323-2638597)


    ENSEMBL Gene Tree for HARS (if available)
    TreeFam Gene Tree for HARS (if available) 

    Paralogs
    for HARS gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for HARS gene
    HARS22  
    3 SIMAP similar genes for HARS using alignment to 9 protein entries:     SYHC_HUMAN (see all proteins):
    HARSL    HARS2    HRS

    HARS for paralogs           About GeneDecksing



    Genomic Variants
    for HARS gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/361 NCBI SNPs in HARS are shown (see all 361)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 5 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs1120299881,2
    		--135198725(+) CACACG/ACAGAC 4 -- ds50011Minor allele frequency- A:0.50CSA 2
    rs775760831,2
    		C,F,--135198802(+) TGCGGG/AGCCCT 4 -- ut311Minor allele frequency- A:0.03WA 118
    rs10502221,2
    		C--135198811(-) CCCATC/TTGCAG 4 -- ut31 ese31Minor allele frequency- T:0.00NA 2
    rs1136271771,2
    		C,--135200099(+) CTTGGG/TCATAA 4 -- int10--------
    rs764961511,2
    		C,F,--135200209(+) AGTAAC/TTTCAA 4 -- int11Minor allele frequency- T:0.02WA 118
    rs737917471,2
    		C,--135200394(+) AAAAAT/ATTTTT 4 -- int11Minor allele frequency- A:0.50WA 2
    rs737917481,2
    		C,--135200886(+) AGTCAC/TGGTAG 4 -- int12Minor allele frequency- T:0.03WA 120
    rs775859831,2
    		--135201074(+) CTGGAA/TAATGC 4 -- int11Minor allele frequency- T:0.01NA 120
    rs113441571,2
    		C,--135201388(+) CCCTG-/CCCAAA 4 -- int10--------
    rs119545141,2
    		C,F,H,--135202016(+) CCTGGA/CCTCTA 4 -- int119Minor allele frequency- C:0.06NA NS EA 2046

    HapMap Linkage Disequilibrium report for HARS (140052758 - 140071609 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for HARS: --
    Human Gene Mutation Database (HGMD): HARS

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing HARS
    DNA2.0 Custom Variant and Variant Library Synthesis for HARS

    Disorders / Diseases
    for HARS gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    HARS for disorders           About GeneDecksing

    OMIM gene information: 142810    OMIM disorders: --

    UniProtKB/Swiss-Prot: SYHC_HUMAN, P12081
  • Defects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized
    • by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet
    syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous
    condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and
    differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2)
    and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular
    dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and
    loss of central visual acuity, usually by the second decade of life
  • Note=HARS mutations may be involved in peripheral neuropathy, a disease mainly characterized by distal motor
    • and sensory dysfunction. Inherited peripheral neuropathies are clinically and genetically heterogeneous with variable
    age of onset and reduced penetrance associated with specific loci. HARS mutations may directly predispose patients to
    peripheral neuropathy or may modify a peripheral neuropathy phenotype by contributing to the genetic and environmental
    load in a given patient (PubMed:22930593)

    20 diseases for HARS:    About MalaCards
    polymyositis    dermatomyositis    facial hemiatrophy    liver angiosarcoma
    interstitial lung disease    systemic scleroderma    myositis    scleroderma
    angiosarcoma    laryngitis    dermatitis    pneumonia
    schizophrenia    tuberculosis    malaria    carcinoma
    usher syndrome    usher syndrome type 3    charles bonnet syndrome    mycobacterium tuberculosis

    2 diseases from the University of Copenhagen DISEASES database for HARS:
    Androgen insensitivity syndrome     Generalized anxiety disorder

    2 Novoseek disease relationships for HARS gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    myositis 78.2 11 9287147 (4), 1688885 (2), 17665459 (1)
    polymyositis 65.8 1 12235211 (1)

    Human Genome Epidemiology (HuGE) Navigator: HARS (1 document)

    Export disorders for HARS gene to outside databases

    Publications
    for HARS gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for HARS gene, integrated from 9 sources (see all 47):
    (articles sorted by number of sources associating them with HARS)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. A novel gene oriented in a head-to-head configuration with the human histidyl-tRNA synthetase (HRS) gene encodes an mRNA that predicts a polypeptide homologous to HRS. (PubMed id 7755634)1, 2, 9 O'Hanlon T.P.... Miller F.W. (1995)
    2. Transcriptional analyses of the gene region that encodes human histidyl-tRNA synthetase: identification of a novel bidirectional regulatory element. (PubMed id 8406012)1, 2, 9 Tsui H.W.... Tsui F.W.L. (1993)
    3. Human histidyl-tRNA synthetase: recognition of amino acid signature regions in class 2a aminoacyl-tRNA synthetases. (PubMed id 1549469)1, 2, 9 Raben N.... Plotz P. (1992)
    4. Genetic mapping and exome sequencing identify variants associated with five novel diseases. (PubMed id 22279524)1, 2 Puffenberger E.G....Strauss K.A. (2012)
    5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    7. Isolation, structure and expression of mammalian genes for histidyl- tRNA synthetase. (PubMed id 3554142)1, 2 Tsui F.W.L. and Siminovitch L. (1987)
    8. Novel conformation of histidyl-transfer RNA synthetase in the lung: the target tissue in Jo-1 autoantibody-associated myositis. (PubMed id 17665459)1, 9 Levine S.M....Casciola-Rosen L.A. (2007)
    9. Cellular localization of the target structures recognized by the anti-Jo-1 antibody: immunofluorescence studies on cultured human myoblasts. (PubMed id 2023220)1, 9 Shi M.H....Rubin L.A. (1991)
    10. A loss-of-function variant in the human histidyl-tRNA synthetase (HARS) gene is neurotoxic in vivo. (PubMed id 22930593)2 Vester A.... Antonellis A. (2013)

    External Searches for HARS gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing HARS gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 3035 HGNC: 4816 AceView: HARS Ensembl:ENSG00000170445 euGenes: HUgn3035
    ECgene: HARS Kegg: 3035 H-InvDB: HARS

    Other Databases showing HARS gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing HARS gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for HARS Pharmacogenomics, SNPs, Pathways

    Intellectual Property
    for HARS gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for HARS gene:
    Search GeneIP for patents involving HARS

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    Products
    for HARS gene

    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
    About This Section

     
     EMD Millipore Custom Antibody & Bulk Services
     EMD Millipore Preclinical / Clinical Development Services
     EMD Millipore Immunoassay Services
     EMD Millipore Target Screening & Profiling Services

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Kinase Activity Assays/Reagents  
     Browse ELISpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Multiplex/Array Assay Kits/Reagents  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Phosphatase Activity Assays/Reagents   Browse Recombinant/Natural Proteins  
     Browse OriGene Antibodies   OriGene shRNA RFP for HARS  
     OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for HARS   OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HARS  
     OriGene Protein Over-expression Lysate for HARS   Browse OriGene Fluorogenic Cell Assay Kits  
     OriGene siRNA for HARS   OriGene 3'-UTR Clone for HARS  
     OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HARS   OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HARS  
     Browse OriGene GFP tagged cDNA clones in CMV expression vector   Browse OriGene MicroRNA Expression Plasmids  
     Browse OriGene basic RS shRNAs   Browse OriGene validated miRNA SYBR primer pairs  
     Browse OriGene full length recombinant human proteins expressed in human HEK293 cells   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for HARS   OriGene Custom Protein Services for HARS  
     OriGene Custom Immunoassay Development  

         		 
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat HARS
     QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing HARS
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HARS
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat HARS
     QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HARS
     QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HARS
     GenScript Custom Purified and Recombinant Proteins Services for HARS GenScript cDNA clones with any tag delivered in your preferred vector for HARS
     GenScript Custom Assay Services for HARS GenScript Custom Superior Antibodies Services for HARS
     GenScript Custom overexpressing Cell Line Services for HARS CloneReady with Over 120,000 Genes
     Gene Synthesis: Any Gene in Any Vector Vector-based siRNA and miRNA, Ready for Transfection
     Gene Mutant Library, Variants up to 10^11 Plasmid Preparation
     Custom Peptide Services
     Search for Antibodies & Assays

     Regulatory tfbs in HARS promoter
     Search Chromatin IP Primers for HARS
     RT2 qPCR Primer Assay in human, mouse, rat HARS
     GNC Network for HARS
     SABiosciences Custom PCR Arrays for HARS
     Search Tocris compounds for HARS
     Browse Sino Biological Proteins and Antibodies
     cDNA Clones for HARS
     Antibodies/Proteins Production Services
     Rabbit Monoclonal Antibody Platform
     Bulk Purchasing
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
     HARS antibodies
     HARS proteins
     HARS lysates
     Antibodies for HARS
     See all of Abcam's Antibodies, Kits and Proteins for HARS
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Recombinant Protein for HARS




     HARS Proteins, Antibodies, CLIAs, and ELISAs
     Search LifeMap BioReagents cell lines for HARS
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HARS
     SwitchGear 3'UTR luciferase reporter plasmids for HARS
     SwitchGear Promoter luciferase reporter plasmids for HARS
     ThermoFisher Antibodies for HARS
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HARS
           
    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 27 Apr 2013

    View Random Gene

    Category
    VWF
    (GIFTS: 73)
    von Willebrand factor
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 

    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Copyright © 1996-2013 , Weizmann Institute of Science. All Rights Reserved.
    Hot genes      Disease genes      HARS gene at Home site.
    hostname: 356980-web2.xennexinc.com index build: 100 solr: 1.4