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HAPLN2 Gene

protein-coding   GIFtS: 46

GC01P154855
hyaluronan and proteoglycan link protein 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
BRAL1 2, 3
Bral1 1
OTTHUMP00000031987 2
Descriptions
Brain link protein 1 3
brain link protein-1 2
hyaluronan and proteoglycan link protein 2 2
External Ids
HGNC: 174101
Entrez Gene: 604842
UniProtKB: Q9GZV73
Ensembl: ENSG000001327027
Search outside databases for aliases for HAPLN2 gene

Previous GC identifers: GC01P153806 GC01P153402

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: HPLN2_HUMAN, Q9GZV7
Function: Mediates a firm binding of versican V2 to hyaluronic acid. May play a pivotal role in the
formation of the hyaluronan-associated matrix in the central nervous system (CNS) which
facilitates neuronal conduction and general structural stabilization. Binds to hyaluronic acid (By
similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the HAPLN2 gene  

Entrez Gene cytogenetic band: 1q23.1   Ensembl cytogenetic band:  1q23.1   HGNC cytogenetic band: 1q23.1

HAPLN2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P154855:     (about GC identifiers)

Start:
154,855,710 bp from pter
End:
154,862,141 bp from pter
Size:
6,432 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000001.9  NT_004487.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: HPLN2_HUMAN, Q9GZV7 (See protein sequence)
Recommended Name: Hyaluronan and proteoglycan link protein 2 precursor  
Size: 340 amino acids; 37775 Da
Subcellular location: Secreted, extracellular space, extracellular matrix (By similarity)

REFSEQ proteins: NP_068589.1  

ENSEMBL proteins: 
ENSP00000255039 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Browse Abcam for Recombinant Proteins
Human Recombinant Proteins from Abnova (HAPLN2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005576 extracellular region IEA--
GO:0005578 proteinaceous extracellular matrix IEA--
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Antibodies for HAPLN2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for HAPLN2
Browse R&D Systems for Antibodies
Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Monoclonal and Polyclonal Antibodies from Abnova (HAPLN2)
Novus Biologicals Antibody for HAPLN2

Assays for HAPLN2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

5/6 InterPro domains/families (see all 6 ):
 IPR007110 Ig-like
 IPR003599 Ig_sub
 IPR016186 C-type_lectin-like
 IPR013783 Ig-like_fold
 IPR013106 Ig_V-set


   GeneDecks  HAPLN2 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9GZV7

ProtoNet protein and cluster: Q9GZV7

3 Blocks protein families:
IPB000538 Link domain
IPB003599 Immunoglobulin subtype
IPB013106 Immunoglobulin V-set


UniProtKB/Swiss-Prot: HPLN2_HUMAN, Q9GZV7
Similarity: Belongs to the HAPLN family
Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain
Similarity: Contains 2 Link domains

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (HAPLN2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (HAPLN2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_021817

              Applied Biosystems Silencer® siRNAs for HAPLN2

              Sigma-Aldrich siRNA for HAPLN2  
                     Sigma-Aldrich shRNA Panels and shRNA for HAPLN2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_021817
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_021817
                                 untagged cDNA clone in CMV expression vector: NM_021817 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_021817

UniProtKB/Swiss-Prot: HPLN2_HUMAN, Q9GZV7
Function: Mediates a firm binding of versican V2 to hyaluronic acid. May play a pivotal role in the
formation of the hyaluronan-associated matrix in the central nervous system (CNS) which
facilitates neuronal conduction and general structural stabilization. Binds to hyaluronic acid (By
similarity)

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005488 binding IEA--
GO:0005540 hyaluronic acid binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


  --

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007155 cell adhesion IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for HAPLN2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (HAPLN2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (HAPLN2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_021817

              Sigma-Aldrich siRNA for HAPLN2  
                     Sigma-Aldrich shRNA Panels and shRNA for HAPLN2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_021817  

REFSEQ mRNAs for HAPLN2 gene: 

NM_021817.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_021817  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_021817
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_021817
                                 untagged cDNA clone in CMV expression vector: NM_021817 

Additional cDNA sequence: 

AB049054.1 AK090712.1 AK094121.1 AK293797.1 AK294131.1 AK308805.1 AK308980.1 AK310758.1 
BC029864.1 

12 DOTS entries:

DT.40250642  DT.427521  DT.97817261  DT.121348924  DT.99998042  DT.121348922  DT.75148902  DT.95162717 
DT.100020273  DT.121348907  DT.121348908  DT.121348914 

24/47 AceView cDNA sequences (see all 47 ):

NM_021817 AI459802 CF457207 BM548424 BC029864 AK090712 BI489912 BM921096 
CB153631 BI757253 BM807774 AB049054 R88039 BF594173 BV206517 H43809 
BI824586 BI821339 BI757758 BU732720 AI460086 CB054443 BM709346 CF455747 

highest scoring ESTs for HAPLN2:

AI973128 AB049054 AI423465 AI459802 AI873583 AK090712 AW015789 BC029864 BE857145 BF594173 

Unigene Cluster for HAPLN2:

Hyaluronan and proteoglycan link protein 2
Hs.410719  [show with all ESTs]
Unigene Representative Sequence: AK094121


GeneLoc Exon Structure

5/15 Alternative Splicing Database (ASD) splice patterns (SP) for HAPLN2 (see all 15 )

ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g · 1h · 1i ^ 2a · 2b · 2c · 2d · 2e · 2f · 2g · 2h ^ 3a · 3b · 3c · 3d ^ 4a · 4b · 4c · 4d ^ 5a ·
SP1:                                                        -     -                                               -     -     -                                 
SP2:                                                        -     -                                               -     -     -                                 
SP3:                                                                                                              -     -     -                                 
SP4:                                                        -     -     -     -     -     -     -                 -     -     -                                 
SP5:                                                        -     -                                               -     -     -     -     -     -     -         

ExUns: 5b · 5c · 5d · 5e · 5f ^ 6a · 6b ^ 7 ^ 8
SP1:                                                      
SP2:                                -                     
SP3:                                                      
SP4:                                                      
SP5:                                                      

About this scheme

ECgene alternative splicing isoforms for HAPLN2

1 Ensembl transcript including schematic representation:
ENST00000255039  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

HAPLN2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for HAPLN2

1 / 2 / 3

3 probe-sets matching HAPLN2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
77456_at2, 3 U95-E 1 1.00 1.00 1.00 1.00 AI973128 1.00 1.00 1.00 1

220142_at2, 3 U133-A 1 1.00 1.00 -- -- NM_021817 0.60 1.00 0.82 1

220142_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  HAPLN2 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.410719

UniProtKB/Swiss-Prot: HPLN2_HUMAN, Q9GZV7
Tissue specificity: Expressed only in adult brain

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for HAPLN2 gene from 5/6 species (see all 6 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
HAPLN21   -- hyaluronan and proteoglycan link protein 2 88.42(n)
92.13(a)
490411  XM_547532.2  XP_547532.2 
cow
(Bos taurus)
HAPLN21   -- hyaluronan and proteoglycan link protein 2 91.25(n)
92.33(a)
514791  NM_001075684.1  NP_001069152.1 
rat
(Rattus norvegicus)
Hapln21   -- hyaluronan and proteoglycan link protein 2 88.1(n)
91.45(a)
64057  NM_022285.1  NP_071621.1 
mouse
(Mus musculus)
Hapln21, 5 3 (42.75 cM)5
hyaluronan and proteoglycan link protein 21, 5 87.51(n)1
92.04(a)1
739401  NM_022031.11  NP_071314.11 
 AB0490555  AK0150345  (see all 11)
chicken
(Gallus gallus)
HAPLN21   -- hyaluronan and proteoglycan link protein 2 70.65(n)
67.1(a)
425975  XM_423654.2  XP_423654.2 
About this table        Species with no ortholog for HAPLN2

ENSEMBL Gene Tree for HAPLN2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for HAPLN2 gene
HAPLN42  HAPLN12  HAPLN32  NCAN2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/72 NCBI SNPs in HAPLN2 are shown (see all 72 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 22)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs23657141,2
A,C,F,H,O154855373(+) CTTCTA/GTCCTC 1 -- ng5112Minor allele frequency- N:0.00EU EA WA NA 1056
rs46612061,2
C,F,H154862641(+) TTGCGG/TAAGGC 1 -- ng314Minor allele frequency- T:0.01EU EA WA 414
--
rs96623731,2
A,C154855103(+) ggcagA/Gagaat 1 -- ng510--------
--
rs75414621,2
A,C154854951(+) agcacG/Tttggg 1 -- ng510--------
--
rs75389611,2
A,C154854904(+) aaaaaA/TTTTTA 1 -- ng51 tfbs3 trp30--------
--
rs75415491,2
A,C154855063(+) ggtggC/Tgggtg 1 -- ng510--------
--
rs75391731,2
A,C154855112(+) atggcA/Gtgaac 1 -- ng510--------
--
rs75270801,2
A,C154854639(+) ttgaaC/Gccggg 1 -- ng510--------
rs11356461,2
A,F154862071(+) CGCCGA/GGGGGA 1 -- ut31 ese3 trp31Minor allele frequency- G:0.01MN 184
--
rs46381221,2
A154853856(+) cctccC/Tgggtt 1 -- ng510--------
About this table

HapMap Linkage Disequilibrium images for HAPLN2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

  --

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

8 PubMed articles for HAPLN2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 60484 HGNC: 17410 AceView: HAPLN2 Ensembl:ENSG00000132702 euGenes: HUgn60484
ECgene: HAPLN2 H-InvDB: HAPLN2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for HAPLN2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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 Predesigned and custom siRNAs for HAPLN2 Antibodies for HAPLN2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for HAPLN2 Browse small molecules at Sigma-Aldrich
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