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HAO2 Gene

protein-coding   GIFtS: 54

GC01P119623
hydroxyacid oxidase 2 (long chain)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 1.1.3.15 3
GIG16 1, 2
HAOX2 1, 2, 3, 5
OTTHUMP00000014486 2
OTTHUMP00000059527 2
Descriptions
(S)-2-hydroxy-acid oxidase, peroxisomal 2, 3
Long chain alpha-hydroxy acid oxidase 3
Long-chain L-2-hydroxy acid oxidase 2, 3
glycolate oxidase 2
growth-inhibiting protein 16 2
hydroxyacid oxidase 2 2
hydroxyacid oxidase 2 (long chain) 2
External Ids
HGNC: 48101
Entrez Gene: 511792
UniProtKB: Q9NYQ33
Ensembl: ENSG000001168827
Search outside databases for aliases for HAO2 gene

Previous GC identifers: GC01P120358 GC01P118749 GC01P119058 GC01P119257

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for HAO2:
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in
encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular
location of the encoded protein is the peroxisome. Specifically, this gene is expressed
predominantly in liver and kidney and has the highest activity toward the substrate
2-hydroxypalmitate. Two alternatively spliced variants encoding the same isoform have been
described. [provided by RefSeq]

UniProtKB/Swiss-Prot: HAOX2_HUMAN, Q9NYQ3
Function: Catalyzes the oxidation of L-alpha-hydroxy acids as well as, more slowly, that of
L-alpha-amino acids

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the HAO2 gene  

Entrez Gene cytogenetic band: 1p13.3-p13.1   Ensembl cytogenetic band:  1p12   HGNC cytogenetic band: 1p13.3-p13.1

HAO2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P119623:     (about GC identifiers)

Start:
119,712,925 bp from pter
End:
119,738,274 bp from pter
Size:
25,350 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000001.9  NT_019273.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: HAOX2_HUMAN, Q9NYQ3 (See protein sequence)
Recommended Name: Hydroxyacid oxidase 2  
Size: 351 amino acids; 38839 Da
Cofactor: FMN
Subunit: Homotetramer or homooctamer (By similarity)
Subcellular location: Peroxisome
Secondary accessions: Q9UJS6

REFSEQ proteins (2 alternative transcripts): 
NP_001005783.1  NP_057611.1  


ENSEMBL proteins: 
ENSP00000358428 ENSP00000354314 ENSP00000316339 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (HAO2)
Human Recombinant Proteins from Abnova (HAO2)
              OriGene Purified Recombinant Human Protein: HAO2 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005777 peroxisome TAS10777549
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Antibodies for HAO2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (HAO2), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (HAO2)
Novus Biologicals Antibody for HAO2

Assays for HAO2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5 InterPro domains/families:
 IPR013785 Aldolase_TIM
 IPR017934 FMN-dep_OHA_DH
 IPR012133 a-Hydoxy_acid_DH_FMN
 IPR008259 FMN_hydac_DH_AS
 IPR000262 FMN-dep_DH


   GeneDecks  HAO2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9NYQ3

ProtoNet protein and cluster: Q9NYQ3

1 Blocks protein family: IPB000262 FMN-dependent alpha-hydroxy acid dehydrogenase

UniProtKB/Swiss-Prot: HAOX2_HUMAN, Q9NYQ3
Similarity: Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family
Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (HAO2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001005783

              Applied Biosystems Silencer® siRNAs for HAO2

              Sigma-Aldrich siRNA for HAO2  
                     Sigma-Aldrich shRNA for HAO2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for HAO2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001005783
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001005783
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001005783 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001005783

UniProtKB/Swiss-Prot: HAOX2_HUMAN, Q9NYQ3
Function: Catalyzes the oxidation of L-alpha-hydroxy acids as well as, more slowly, that of
L-alpha-amino acids
Catalytic activity: (S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)
Enzyme Number (IUBMB): EC 1.1.3.15 

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003973 (S)-2-hydroxy-acid oxidase activity TAS10777549
GO:0009055 electron carrier activity IEA--
GO:0010181 FMN binding IEA--
GO:0016491 oxidoreductase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

1 Invitrogen iPath™ Online BioAtlas - Pathway for HAO2 (Maps provided by GeneGo):
 Alanine, serine, cysteine, threonine and glycine metabolism

   GeneDecks  HAO2 for the pathways selected above  
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  HAO2  (Your Favorite Gene powered by Ingenuity) 
 Glyoxylate and Dicarboxylate Metabolism

   GeneDecks  HAO2 for the pathways selected above  
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1 Kegg Pathway  (Kegg details for HAO2):
 hsa00630 Glyoxylate and dicarboxylate metabolism

   GeneDecks  HAO2 for the pathways selected above  
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4 Interacting proteins for HAO2 (ENSP000003584283) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
AGXTENSP000003026203STRING (score=.878)
HAO1ENSP000003680663STRING (score=.819)
AGXT2ENSP000002314203STRING (score=.8)
DAOENSP000002284763STRING (score=.8)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0001561 fatty acid alpha-oxidation TAS10777549
GO:0055114 oxidation reduction IEA--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for HAO2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (HAO2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001005783

              Sigma-Aldrich siRNA for HAO2  
                     Sigma-Aldrich shRNA for HAO2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001005783  NM_016527  

REFSEQ mRNAs for HAO2 gene (2 alternative transcripts): 

NM_001005783.1   NM_016527.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001005783  NM_016527  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001005783
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001005783
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001005783 

Additional cDNA sequence: 

AF203975.1 AF231917.1 AK298289.1 AY513277.1 BC020863.1 

6 DOTS entries:

DT.40123369  DT.100784731  DT.100019080  DT.91711673  DT.100784732  DT.95246230 

24/62 AceView cDNA sequences (see all 62 ):

AI733261 BE464431 BG564465 AW139221 AA969532 CB146914 CB162598 BG428569 
AI373640 AI793285 CB148815 CB147318 BG428162 CB161619 BE048784 BG402318 
CB148854 W78813 BG431374 CB146648 AA962446 AI636238 AI572851 CB121424 

highest scoring ESTs for HAO2:

AW024657 AW025423 AA701282 AA703164 AA919149 AF203975 AF231917 AI271956 AI476213 AI795958 

Unigene Cluster for HAO2:

Hydroxyacid oxidase 2 (long chain)
Hs.659767  [show with all ESTs]
Unigene Representative Sequence: AK298289


GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for HAO2

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7 ^ 8 ^ 9a · 9b · 9c
SP1:                                                                                                
SP2:                    -                                                                           
SP3:                    -                 -                                                         
SP4:                                                        -     -                                 

About this scheme

ECgene alternative splicing isoforms for HAO2

3 Ensembl transcripts including schematic representations:
ENST00000369420  ENST00000361035  ENST00000325945  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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HAO2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for HAO2

1 / 2 / 3

4 probe-sets matching HAO2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
90243_f_at2, 3 U95-D 1 1.00 1.00 0.99 1.11 AW025423 1.00 1.00 1.00 1
87921_f_at2, 3 U95-E 1 0.88 1.00 0.99 0.89 AW024657 1.00 1.00 1.00 1

220801_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_016527 0.60 1.00 0.82 1

220801_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  HAO2 for binary patterns associated with the probe-sets selected above  
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About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CCCTTCTTTG

SOURCE GeneReport for Unigene cluster: Hs.659767

UniProtKB/Swiss-Prot: HAOX2_HUMAN, Q9NYQ3
Tissue specificity: Liver and kidney

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for HAO2 gene from 5/14 species (see all 14 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
HAO21   -- hydroxyacid oxidase 2 (long chain) 85.75(n)
82.34(a)
475814  XM_533023.2  XP_533023.2 
cow
(Bos taurus)
HAO21   -- hydroxyacid oxidase 2 (long chain) 83.95(n)
82.91(a)
509481  NM_001035071.1  NP_001030243.1 
rat
(Rattus norvegicus)
Hao21   -- hydroxyacid oxidase 2 (long chain) 79.87(n)
74.07(a)
84029  NM_032082.1  NP_114471.1 
mouse
(Mus musculus)
Hao25
Hao31
3 (39.60 cM)5
hydroxyacid oxidase 25
hydroxyacid oxidase (glycolate oxidase) 31
78.63(n)1
71.51(a)1
561851  NM_019545.31  NP_062418.21 
 AF2729475  AI3254785  (see all 12)
chicken
(Gallus gallus)
HAO21   -- hydroxyacid oxidase 2 (long chain) 63.63(n)
60.11(a)
418311  XM_416535.2  XP_416535.2 
About this table        Species with no ortholog for HAO2

ENSEMBL Gene Tree for HAO2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for HAO2 gene
HAO12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/161 NCBI SNPs in HAO2 are shown (see all 161 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 78)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs37532621,2
A,C,F,H119711602(-) ggaacA/Ttggtg 2 -- ng5111Minor allele frequency- T:0.38EU EA WA NA 978
rs37532631,2
A,C,F,H119711372(-) ATTGCA/TCTGAG 2 -- ng5117Minor allele frequency- N:0.00EA EU WA NA 2952
rs37532641,2
A,C,F,H119711037(-) TGACCC/TAGTCT 2 -- ng51 tfbs38Minor allele frequency- T:0.43EA EU WA NA 2044
rs170235071,2
F,H119724770(+) CATGAC/TGTGGG 2 -- int1 ut51 ese37Minor allele frequency- T:0.03NA EU EA WA 550
rs66563601,2
F,H119712028(+) GCTCCG/ATGTGT 2 -- ng514Minor allele frequency- A:0.04EU EA WA 408
rs37532611,2
F,H119712012(-) AGGCCG/AAAATC 2 -- ng514Minor allele frequency- A:0.07EU EA WA 420
rs346382611,2
C,F119725274(+) CGCGAG/AAGCAG 2 K/E mis15Minor allele frequency- A:0.04NA EU EA WA 498
rs66616251,2
H119730867(+) TCATCC/ATGAAA 2 M/L mis14Minor allele frequency- A:0.00EU EA WA 404
rs38200311,2
H119711675(-) CCTCCC/GTTGCT 2 -- ng514Minor allele frequency- G:0.00EU EA WA 418
rs38189271,2
H119738323(+) ATGAAA/GTGACT 2 -- ng314Minor allele frequency- G:0.00EU EA WA 420
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HapMap Linkage Disequilibrium images for HAO2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 605176

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

4 PubMed articles for HAO2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 51179 HGNC: 4810 AceView: HAO2 Ensembl:ENSG00000116882 euGenes: HUgn51179
ECgene: HAO2 H-InvDB: HAO2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for HAO2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for HAO2-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
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 Predesigned and custom siRNAs for HAO2 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for HAO2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant Proteins
(HAO2)
 Antibodies (HAO2)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for HAO2




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for HAO2

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