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 | Services
| |
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Aliases &
Descriptions
for H3F3B
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases |
|---|
| H3.3A 3 | | H3.3B 1, 2, 3 | | H3F3 3 | | H3F3A 2 |
| | | Descriptions |
|---|
| H3 histone, family 3A 2 | | H3 histone, family 3B 2 | | H3 histone, family 3B (H3.3B) 2 |
|
| |
Search outside databases for aliases for H3F3B genePrevious GC identifers: GC17M073749 GC17M076798 GC17M074237 GC17M074371 GC17M074372 |
Summaries
for H3F3B(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for H3F3B:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the
chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3,
and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units,
called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and
functions in the compaction of chromatin into higher order structures. This gene contains introns
and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the
histone H3 family. [provided by RefSeq]
UniProtKB/Swiss-Prot: H33_HUMAN, P84243Function: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in
active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is
incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal
displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of
transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA
accessibility to the cellular machineries which require DNA as a template. Histones thereby play a
central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
DNA accessibility is regulated via a complex set of post-translational modifications of histones,
also called histone code, and nucleosome remodeling |
Genomic Location
for H3F3B
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences)
About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
 Transcription factor binding sites upstream to the H3F3B gene 
Entrez Gene cytogenetic band: 17q25 Ensembl cytogenetic band: 17q25.1 HGNC cytogenetic band: 17q25.1H3F3B Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 17 GeneLoc Exon Structure GeneLoc location for GC17M071284:
(about GC identifiers)
Start:
|
71,284,110 bp from pter |
End:
|
71,287,455 bp from pter |
Size:
|
3,346 bases |
Orientation:
|
minus strand |
RefSeq DNA sequence:- NC_000017.9 NT_010641.15
| Proteins
for H3F3B
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: H33_HUMAN, P84243 (See
protein sequence)Recommended Name: Histone H3.3 Size: 136 amino acids; 15328 Da
Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4
assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps
approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into
nucleosomes
Subcellular location: Nucleus
Developmental stage: Expressed throughout the cell cycle independently of DNA synthesis
Sequence caution: Sequence=CAH73371.1; Type=Erroneous gene model prediction;
Secondary accessions: P06351 P33155 Q5VV55 Q5VV56 Q66I33 Q9V3W4Post-translational modifications:
Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs
methylation at Arg-9 (H3R8sme2). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors
methylation at Arg-18 (H3R17me)1
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses
transcription1
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric
dimethylation at Arg-9 (H3R8sme2) by PRMT5 is linked to gene repression. Asymmetric dimethylation
at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5
methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their
transcription state and is enriched on inactive promoters, while it is absent on active promoters1
Specifically enriched in modifications associated with active chromatin such as methylation at
Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent
acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand
break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and
Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10
(H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent
phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me)
and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at
Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin1
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during
anaphase. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early
anaphase, probably DAPK3. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome
condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at
Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the
transcription of genes following external stimulation, like mitogens, stress, growth factors or UV
irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at
Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but
facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the
dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11
(H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation.
Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B
irradiation. Phosphorylation on Ser-32 is specific to regions bordering centromeres in metaphase
chromosomes1
Ubiquitinated (By similarity)1
REFSEQ proteins: NP_005315.1
ENSEMBL proteins: ENSP00000254810
Human Recombinant Proteins  OriGene Purified Recombinant Human Protein: H3F3B
4 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for H3F3B:
Assays for H3F3B: | Protein
Domains/
Families
for H3F3B(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry P84243
ProtoNet protein and cluster: P84243 UniProtKB/Swiss-Prot: H33_HUMAN, P84243Similarity: Belongs to the histone H3 family | Gene Function
for H3F3B
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005324
 Applied Biosystems Silencer® siRNAs for H3F3B
 Sigma-Aldrich siRNA for H3F3B
Sigma-Aldrich shRNA for H3F3B
Explore Sigma-Aldrich super-pooled esiRNAs
OriGene GFP tagged cDNA clone in CMV expression vector: NM_005324
Myc/DDK tagged cDNA clone in CMV expression vector: NM_005324
untagged cDNA clone in CMV expression vector: NM_005324
Primers:  Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
OriGene genome-wide validated SYBR primer pairs: NM_005324
UniProtKB/Swiss-Prot: H33_HUMAN, P84243Function: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in
active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is
incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal
displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of
transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA
accessibility to the cellular machineries which require DNA as a template. Histones thereby play a
central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
DNA accessibility is regulated via a complex set of post-translational modifications of histones,
also called histone code, and nucleosome remodelingGenatlas biochemistry entry for H3F3B:histone family 3,replacement subtype,replication independent protein,species H3.3B1 Gene Ontology (GO) molecular function term (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0003677 | DNA binding |
IEA | -- |
About this table | Pathways & Interactions
for H3F3B
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
3 Sigma-Aldrich "Your Favorite Gene" Pathways for H3F3B (Your Favorite Gene powered by Ingenuity)
1 Gene Ontology (GO) biological process term (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006334 | nucleosome assembly |
IEA | -- |
About this table
|
Drugs & Compounds
for H3F3B(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
 Browse Tocris compounds for H3F3B
2 PharmGKB drug compound relationships for H3F3B geneAbout this table
|
Transcripts
for H3F3B(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene,
Expression Assays from Applied Biosystems)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005324
Sigma-Aldrich siRNA for H3F3B
Sigma-Aldrich shRNA for H3F3B
Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer® siRNAs: NM_005324 REFSEQ mRNAs for H3F3B gene: NM_005324.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_005324
OriGene GFP tagged cDNA clone in CMV expression vector: NM_005324
Myc/DDK tagged cDNA clone in CMV expression vector: NM_005324
untagged cDNA clone in CMV expression vector: NM_005324
Additional cDNA sequence: AF218029.1 AK054591.1 AK130772.1 AK304607.1 AK312762.1 BC001124.2 BC006497.2 BC012813.2 BC017558.1 BC020466.1 BC108701.1 BX537379.1 BX647280.1 CR591387.1 CR591461.1 CR595062.1 CR595544.1 CR595774.1 CR597711.1 CR599200.1 CR602412.1 CR602581.1 CR602780.1 CR603175.1 CR603793.1 CR603889.1 CR604778.1 CR605171.1 CR606388.1 CR608885.1 CR610507.1 CR611244.1 CR613143.1 CR614120.1 CR615382.1 CR616111.1 CR617433.1 CR618076.1 CR618082.1 CR619729.1 CR621009.1 CR621264.1 CR622202.1 CR622564.1 CR622957.1 CR624690.1 CR625366.1 CR625547.1 24/85 DOTS entries (see all 85
): DT.91778033 DT.91778014 DT.91778022 DT.100706978 DT.91778028 DT.100706984 DT.100837098 DT.100707000 DT.120913319 DT.92325522 DT.100706983 DT.120913311 DT.102839701 DT.120913435 DT.100706990 DT.100706989 DT.87017903 DT.91986813 DT.100706982 DT.100058702 DT.100707002 DT.120913371 DT.40115786 DT.100706993 24/3236 AceView cDNA sequences (see all 3236
):CD515565 CR597711 AW439308 CB126004 BQ675146 BQ961539 CB121899 AI190042 BI333867 BG944771 BQ876814 AA600343 AW117666 AI375017 AA428357 AA182788 BM764324 AA916825 AI192216 BM762905 BF194757 AA700995 CA449664 N22138
 highest scoring ESTs for H3F3B:AA053483 AA056379 AA126392 AA129005 AA134903 AA143540 AA151436 AA152010 AA196866 AA205021 Unigene Cluster for H3F3B: H3 histone, family 3B (H3.3B) Hs.180877 [show with all ESTs]Unigene Representative Sequence: BX537379
GeneLoc Exon Structure
4 Alternative Splicing Database (ASD) splice patterns (SP) for H3F3B
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b | ^ | 5a | · | 5b | · | 5c |
|---|
|
| SP1: | | | | | - | | | | | | - | | | | | | | | |
| SP2: | | | | | | | | | | | - | | | | | | | | |
| SP3: | | | | | - | | | | | | - | | | | | | | | |
| SP4: | | | | | - | | | | | | | | | | | | | |
About this scheme
ECgene alternative splicing isoforms for H3F3B
1 Ensembl transcript including schematic representation: ENST00000254810
|
Expression
for H3F3B
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| H3F3B expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for H3F3B
1 / 2 / 3 20 probe-sets matching H3F3B gene Data from
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: --
SOURCE GeneReport for Unigene cluster: Hs.180877
Expression variation in blood from EXPOLDB for H3F3B |
Orthologs
for H3F3B
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for H3F3B gene from 5/10 species (see all 10
)
About this table Species with no ortholog for H3F3B
ENSEMBL Gene Tree for H3F3B | Paralogs
for H3F3B(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| Paralogs for H3F3B gene
- H3F3A1 2 LOC4409262
|
SNPs/Variants
for H3F3B(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for H3F3B (up to first 250kb)
|
Disorders & Mutations
for H3F3B
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 601058
1 PharmGKB disease relationship for H3F3B geneAbout this table
|
Medical News
for H3F3B(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for H3F3B (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/55 PubMed articles for H3F3B gene (see all 55
): |
Search for H3F3B
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing H3F3B
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing H3F3B
(According to HUGE)
About This Section
| -- |
Specialized Databases showing H3F3B(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| Wikipedia | http://en.wikipedia.org/wiki/Histone_H3 |
|
| | | About This Section
| --
| Services
for H3F3B(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section
| 
Products for H3F3B:
 | |
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 |
 |  |  | |
 | Antibodies & Assays for H3F3B (Histone H3) |
| | | | | Search Tocris compounds for H3F3B |
|
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Incremental update: 13 Oct 2009
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