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Aliases &
Descriptions
for H3F3A
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases |
|---|
| H3.3A 1, 2, 3 | | H3.3B 3 | | H3F3 2, 3, 5 | | MGC87782 2 | | MGC87783 2 | | OTTHUMP00000035618 2 | | OTTHUMP00000035619 2 | | OTTHUMP00000035621 2 |
| | | Descriptions |
|---|
| H3 histone, family 3A 2 |
|
| |
Search outside databases for aliases for H3F3A genePrevious GC identifers: GC01P224732 GC01P222067 GC01P222656 GC01P223225 GC01P222557 |
Summaries
for H3F3A(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for H3F3A:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the
chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3,
and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units,
called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and
functions in the compaction of chromatin into higher order structures. This gene contains introns
and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a
replication-independent member of the histone H3 family. [provided by RefSeq]
UniProtKB/Swiss-Prot: H33_HUMAN, P84243Function: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in
active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is
incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal
displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of
transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA
accessibility to the cellular machineries which require DNA as a template. Histones thereby play a
central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
DNA accessibility is regulated via a complex set of post-translational modifications of histones,
also called histone code, and nucleosome remodelingGene Wiki entry for H3F3A |
Genomic Location
for H3F3A
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences)
About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
 Transcription factor binding sites upstream to the H3F3A gene 
Entrez Gene cytogenetic band: 1q41 Ensembl cytogenetic band: 1q42.12 HGNC cytogenetic band: 1q42.12H3F3A Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 1 GeneLoc Exon Structure GeneLoc location for GC01P224316:
(about GC identifiers)
Start:
|
224,316,175 bp from pter |
End:
|
224,326,326 bp from pter |
Size:
|
10,152 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000001.9 NT_004559.13
| Proteins
for H3F3A
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: H33_HUMAN, P84243 (See
protein sequence)Recommended Name: Histone H3.3 Size: 136 amino acids; 15328 Da
Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4
assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps
approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into
nucleosomes
Subcellular location: Nucleus
Developmental stage: Expressed throughout the cell cycle independently of DNA synthesis
Sequence caution: Sequence=CAH73371.1; Type=Erroneous gene model prediction;
Secondary accessions: P06351 P33155 Q5VV55 Q5VV56 Q66I33 Q9V3W4Post-translational modifications:
Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs
methylation at Arg-9 (H3R8sme2). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors
methylation at Arg-18 (H3R17me)1
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses
transcription1
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric
dimethylation at Arg-9 (H3R8sme2) by PRMT5 is linked to gene repression. Asymmetric dimethylation
at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5
methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their
transcription state and is enriched on inactive promoters, while it is absent on active promoters1
Specifically enriched in modifications associated with active chromatin such as methylation at
Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent
acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand
break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and
Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10
(H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent
phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me)
and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at
Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin1
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during
anaphase. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early
anaphase, probably DAPK3. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome
condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at
Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the
transcription of genes following external stimulation, like mitogens, stress, growth factors or UV
irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at
Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but
facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the
dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11
(H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation.
Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B
irradiation. Phosphorylation on Ser-32 is specific to regions bordering centromeres in metaphase
chromosomes1
Ubiquitinated (By similarity)1
REFSEQ proteins: NP_002098.1
ENSEMBL proteins: ENSP00000355780 ENSP00000355781 ENSP00000355778 ENSP00000355779
Human Recombinant Proteins  OriGene Purified Recombinant Human Protein: H3F3A
Antibodies for H3F3A:
Assays for H3F3A: | Protein
Domains/
Families
for H3F3A(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry P84243
ProtoNet protein and cluster: P84243 UniProtKB/Swiss-Prot: H33_HUMAN, P84243Similarity: Belongs to the histone H3 family | Gene Function
for H3F3A
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002107
 Applied Biosystems Silencer® siRNAs for H3F3A
 Sigma-Aldrich siRNA for H3F3A
Sigma-Aldrich shRNA for H3F3A
Explore Sigma-Aldrich super-pooled esiRNAs
OriGene GFP tagged cDNA clone in CMV expression vector: NM_002107
Myc/DDK tagged cDNA clone in CMV expression vector: NM_002107
untagged cDNA clone in CMV expression vector: NM_002107
Primers:  Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
OriGene genome-wide validated SYBR primer pairs: NM_002107
UniProtKB/Swiss-Prot: H33_HUMAN, P84243Function: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in
active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is
incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal
displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of
transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA
accessibility to the cellular machineries which require DNA as a template. Histones thereby play a
central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
DNA accessibility is regulated via a complex set of post-translational modifications of histones,
also called histone code, and nucleosome remodelingGenatlas biochemistry entry for H3F3A:histone family 3,replacement subtype,replication independent protein,species H3.3A4 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for H3f3a):
| Pathways & Interactions
for H3F3A
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
3 Sigma-Aldrich "Your Favorite Gene" Pathways for H3F3A (Your Favorite Gene powered by Ingenuity)
5/29 Interacting proteins for H3F3A (ENSP000003557813) via UniProtKB, MINT, and/or STRING (see all 29
)About this table
|
Drugs & Compounds
for H3F3A(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
 Browse Tocris compounds for H3F3A
|
Transcripts
for H3F3A(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene,
Expression Assays from Applied Biosystems)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002107
Sigma-Aldrich siRNA for H3F3A
Sigma-Aldrich shRNA for H3F3A
Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer® siRNAs: NM_002107 REFSEQ mRNAs for H3F3A gene: NM_002107.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_002107
OriGene GFP tagged cDNA clone in CMV expression vector: NM_002107
Myc/DDK tagged cDNA clone in CMV expression vector: NM_002107
untagged cDNA clone in CMV expression vector: NM_002107
Additional cDNA sequence: AK293541.1 CR592641.1 CR594694.1 CR621187.1 24/89 DOTS entries (see all 89
): DT.95139642 DT.100695640 DT.100695654 DT.97864380 DT.100059951 DT.85101791 DT.100695644 DT.95223619 DT.120933375 DT.100046204 DT.120933330 DT.100862784 DT.87018230 DT.120933449 DT.91772344 DT.121236364 DT.92418544 DT.100034237 DT.100695647 DT.121461590 DT.120933504 DT.100864775 DT.120782523 DT.121461589 24/1292 AceView cDNA sequences (see all 1292
):AI335873 AA584020 BM756172 AW161005 BP358114 BP367228 BU679021 CB129484 N22116 AA327928 BC067757 AI439694 AL697957 BI833468 BF724477 CB156722 BG398578 AI273173 AI929536 CR594694 BM044822 CA866060 AA075161 BG481865
 highest scoring ESTs for H3F3A:AA079186 AA160506 AA176227 AA424089 AA449069 AA492234 AA668124 AA775360 AA935582 AI002440 Unigene Clusters for H3F3A: H3 histone, family 3A Hs.533624 [show with all ESTs], Hs.546259 [show with all ESTs]Unigene Representative Sequences: BM907805, CR621187
GeneLoc Exon Structure
4 Ensembl transcripts including schematic representations: ENST00000366815
ENST00000366816
ENST00000366813
ENST00000366814
|
Expression
for H3F3A
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| H3F3A expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for H3F3A
1 / 2 / 3 10 probe-sets matching H3F3A gene Data from
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: --
SOURCE GeneReport for Unigene clusters: Hs.533624 Hs.546259
Expression variation in blood from EXPOLDB for H3F3A |
Orthologs
for H3F3A
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for H3F3A gene from 5/10 species (see all 10
)
About this table Species with no ortholog for H3F3A
ENSEMBL Gene Tree for H3F3A | Paralogs
for H3F3A(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| Paralogs for H3F3A gene
- H3F3B1 2 LOC4409262
|
SNPs/Variants
for H3F3A(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for H3F3A (up to first 250kb)
|
Disorders & Mutations
for H3F3A
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 601128
|
Medical News
for H3F3A(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for H3F3A (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/59 PubMed articles for H3F3A gene (see all 59
):- Unusual structure, evolutionary conservation of non-coding sequences and numerous pseudogenes characterize the human H3.3 histone multigene family. (PubMed id 3031613)2, 3, 4 Wells D.... Kedes L. (1987)
- The human replacement histone H3.3B gene (H3F3B). (PubMed id 8586426)1, 3, 4 Albig W.... Doenecke D. (1995)
- Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification. (PubMed id 17189264)3, 4 Morris S.A.... Strahl B.D. (2007)
- Organismal differences in post-translational modifications in histones H3 and H4. (PubMed id 17194708)3, 4 Garcia B.A.... Hunt D.F. (2007)
- Histone H3.3 deposition at E2F-regulated genes is linked to transcription. (PubMed id 16258499)3, 4 Daury L.... Trouche D. (2006)
- Substrate and functional diversity of lysine acetylation revealed by a proteomics survey. (PubMed id 16916647)3, 4 Kim S.C.... Zhao Y. (2006)
- Expression patterns and post-translational modifications associated with mammalian histone H3 variants. (PubMed id 16267050)3, 4 Hake S.B.... Hunt D.F. (2006)
- Mass spectrometric characterization of human histone H3: a bird's eye view. (PubMed id 16457588)3, 4 Thomas C.E.... Mizzen C.A. (2006)
- The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)3, 4 Gregory S.G.... Bentley D.R. (2006)
- Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17. (PubMed id 16497732)3, 4 Miao F.... Natarajan R. (2006)
|
Search for H3F3A
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing H3F3A
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing H3F3A
(According to HUGE)
About This Section
| -- |
Specialized Databases showing H3F3A(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| Wikipedia | http://en.wikipedia.org/wiki/Histone_H3 |
|
| | | About This Section
| --
| Services
for H3F3A(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section
| 
Products for H3F3A:
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