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H2AFY2 Gene

protein-coding   GIFtS: 50

GC10P071482
H2A histone family, member Y2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
MACROH2A2 3
OTTHUMP00000019735 2
mH2A2 3
macroH2A2 1, 2
Descriptions
H2A histone family, member Y2 2
Histone macroH2A2 3
core histone macroH2A2.2 2
External Ids
HGNC: 144531
Entrez Gene: 555062
UniProtKB: Q9P0M63
Ensembl: ENSG000000992847
Search outside databases for aliases for H2AFY2 gene

Previous GC identifers: GC10P070623 GC10P070899 GC10P071704 GC10P071157

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: H2AW_HUMAN, Q9P0M6
Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it
represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA
accessibility to the cellular machineries which require DNA as a template. Histones thereby play a
central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
DNA accessibility is regulated via a complex set of post-translational modifications of histones,
also called histone code, and nucleosome remodeling. May be involved in stable X chromosome
inactivation

Gene Wiki entry for H2AFY2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the H2AFY2 gene  

Entrez Gene cytogenetic band: 10q22   Ensembl cytogenetic band:  10q22.1   HGNC cytogenetic band: 10q22.1

H2AFY2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P071482:     (about GC identifiers)

Start:
71,482,363 bp from pter
End:
71,542,046 bp from pter
Size:
59,684 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000010.9  NT_008583.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: H2AW_HUMAN, Q9P0M6 (See protein sequence)
Recommended Name: Core histone macro-H2A.2  
Size: 372 amino acids; 40058 Da
Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4
assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers
Subcellular location: Nucleus. Note=Enriched in inactive X chromosome chromatin and in
senescence-associated heterochromatin
Secondary accessions: Q5SQT2

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_061119.1  

    ENSEMBL proteins: 
    ENSP00000362352 ENSP00000378486 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Macro H2A.2)
    Human Recombinant Proteins from Abnova (H2AFY2)
                  OriGene Purified Recombinant Human Protein: H2AFY2 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000786 nucleosome IEA--
    GO:0001740 Barr body IDA11331621
    GO:0005634 nucleus IEA--
    GO:0005694 chromosome IEA--
    About this table

    Antibodies for H2AFY2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Macro H2A.2), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for H2AFY2

    Assays for H2AFY2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR002589 A1pp
     IPR007125 Histone_core_D
     IPR002119 Histone_H2A
     IPR009072 Histone-fold


       GeneDecks  H2AFY2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9P0M6

    ProtoNet protein and cluster: Q9P0M6

    2 Blocks protein families:
    IPB002119 Histone H2A
    IPB002589 Protein of unknown function Appr-1


    UniProtKB/Swiss-Prot: H2AW_HUMAN, Q9P0M6
    Similarity: Contains 1 histone H2A domain
    Similarity: Contains 1 Macro domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (H2AFY2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018649

                  Applied Biosystems Silencer® siRNAs for H2AFY2

                  Sigma-Aldrich siRNA for H2AFY2  
                         Sigma-Aldrich shRNA for H2AFY2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for H2AFY2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018649
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018649
                                     untagged cDNA clone in CMV expression vector: NM_018649 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_018649

    UniProtKB/Swiss-Prot: H2AW_HUMAN, Q9P0M6
    Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it
    represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA
    accessibility to the cellular machineries which require DNA as a template. Histones thereby play a
    central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
    DNA accessibility is regulated via a complex set of post-translational modifications of histones,
    also called histone code, and nucleosome remodeling. May be involved in stable X chromosome
    inactivation

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Kegg Pathway  (Kegg details for H2AFY2):
     hsa05322 Systemic lupus erythematosus

       GeneDecks  H2AFY2 for the pathways selected above  
    About GeneDecksing

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006334 nucleosome assembly IEA--
    GO:0007549 dosage compensation IDA11331621
    GO:0016568 chromatin modification IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for H2AFY2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (H2AFY2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018649

                  Sigma-Aldrich siRNA for H2AFY2  
                         Sigma-Aldrich shRNA for H2AFY2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_018649  

    REFSEQ mRNAs for H2AFY2 gene: 

    NM_018649.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_018649  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018649
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018649
                                     untagged cDNA clone in CMV expression vector: NM_018649 

    Additional cDNA sequence: 

    AF336304.1 AK022475.1 AK022776.1 AK131057.1 AL359572.1 BC016172.2 CR592495.1 CR601217.1 
    CR613322.1 CR613570.1 CR623698.1 

    8 DOTS entries:

    DT.416777  DT.91766758  DT.100714171  DT.102829851  DT.416778  DT.91766762  DT.95312865  DT.106028 

    24/207 AceView cDNA sequences (see all 207 ):

    BQ428558 BC016172 BG055758 BG913090 BM724974 BU741376 BM670635 BM711131 
    AA633441 BI767336 AI435068 BE047110 AA402908 BQ648649 BQ231153 AW136167 
    CN481762 BM682238 CR613570 BE464932 AK131057 CB241177 AA285026 CA390171 

    highest scoring ESTs for H2AFY2:

    AI022248 AA285016 AA285026 AA318946 AA565703 AA581324 AA633441 AA678734 AA702525 AA704931 

    Unigene Cluster for H2AFY2:

    H2A histone family, member Y2
    Hs.499953  [show with all ESTs]
    Unigene Representative Sequence: AL359572


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000373255  ENST00000395046  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    H2AFY2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for H2AFY2

    1 / 2 / 3

    3 probe-sets matching H2AFY2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    65695_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 AI022248 1.00 1.00 1.00 1

    218445_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018649 0.60 1.00 0.82 1

    218445_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TGTTTTCCTC

    SOURCE GeneReport for Unigene cluster: Hs.499953
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for H2AFY2 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LOC4890241   -- similar to Core histone macro-H2A.2 (Histone macroH2A2) more 92.83(n)
    99.19(a)
    489024  XM_856111.1  XP_861204.1 
    chimpanzee
    (Pan troglodytes)
    LOC4505091   -- similar to core histone macroH2A2.2 99.46(n)
    100(a)
    450509  XM_507834.2  XP_507834.2 
    cow
    (Bos taurus)
    H2AFY21   -- H2A histone family, member Y2 93.1(n)
    99.73(a)
    537167  NM_001076086.1  NP_001069554.1 
    rat
    (Rattus norvegicus)
    H2afy21   -- H2A histone family, member Y2 90.77(n)
    98.39(a)
    361844  XM_342138.3  XP_342139.2 
    mouse
    (Mus musculus)
    H2afy21, 5 105
    H2A histone family, member Y21, 5 90.23(n)1
    98.39(a)1
    4046341  NM_207000.21  NP_996883.11 
     AF3363055  AK0183555  (see all 20)
    About this table        Species with no ortholog for H2AFY2

    ENSEMBL Gene Tree for H2AFY2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for H2AFY2 gene
    H2AFV2  H2AFZ2  H2AFY2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/308 NCBI SNPs in H2AFY2 are shown (see all 308 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 164)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 10 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs109991081,2
    C,F,H71481777(+) GGAGCC/ACTGGC 1 -- ng515Minor allele frequency- A:0.12MN EA WA 652
    rs125701651,2
    C,F,H71481818(+) CTTCCA/GCTAGG 1 -- ng51 tfbs36Minor allele frequency- G:0.13MN EA EU WA 776
    --
    rs115542151,2
    H71521630(+) CCCCAC/ACCCCA 1 T/P mis1 ese34Minor allele frequency- A:0.00EU EA WA 392
    rs23946541,2
    A,C,F,H,O71540120(+) CACAAT/CGCTGA 1 -- int121Minor allele frequency- C:0.14NS EU EA WA NA 1504
    rs23109921,2
    A,C,F,H,O71493573(+) GCAGAT/CGGGAG 1 -- int112Minor allele frequency- C:0.30EA NA EU WA 994
    rs22716981,2
    A,C,F,H71538769(-) TGCCGC/TGGGTT 1 -- int19Minor allele frequency- N:0.00EA NA EU WA 2122
    rs79172901,2
    A,C,F,H71536167(+) TGCCAC/TGAACA 1 -- int112Minor allele frequency- N:0.00EU EA WA NA 1034
    rs79147871,2
    A,C,F,H71532660(+) TAGACA/GGAGAC 1 -- int18Minor allele frequency- G:0.36EU EA WA NA 778
    rs47469591,2
    A,C,F,H71520936(+) TCTGGA/TATTCC 1 -- int14Minor allele frequency- T:0.10EU EA WA 404
    rs108234771,2
    A,C,F,H71497070(+) TTCAGG/AGTTAG 1 -- int14Minor allele frequency- A:0.11EU EA WA 410
    About this table

    HapMap Linkage Disequilibrium images for H2AFY2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/11 PubMed articles for H2AFY2 gene (see all 11 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 55506 HGNC: 14453 AceView: H2AFY2 Ensembl:ENSG00000099284 euGenes: HUgn55506
    ECgene: H2AFY2 H-InvDB: H2AFY2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for H2AFY2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for H2AFY2-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for H2AFY2 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for H2AFY2 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Recombinant Proteins
    (Macro H2A.2)
     Antibodies (Macro H2A.2)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for H2AFY2




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Search Novus for antibodies for H2AFY2

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