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H1FX Gene

protein-coding   GIFtS: 56

GC03M130516
H1 histone family, member X
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
H1X 1, 2
MGC15959 1, 2
MGC8350 1, 2
Descriptions
H1 histone family, member X 2
External Ids
HGNC: 47221
Entrez Gene: 89712
UniProtKB: Q925223
Ensembl: ENSG000001848977
Search outside databases for aliases for H1FX gene

Previous GC identifers: GC00U990454 GC03U900028 GC03M130315 GC03M130354

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for H1FX:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the
chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around
a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The
chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA
between the nucleosomes to form higher order chromatin structures. This gene encodes a member of
the histone H1 family. [provided by RefSeq]

UniProtKB/Swiss-Prot: H1X_HUMAN, Q92522
Function: Histones H1 are necessary for the condensation of nucleosome chains into higher order
structures

Gene Wiki entry for H1FX

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the H1FX gene  

Entrez Gene cytogenetic band: 3q21.3   Ensembl cytogenetic band:  3q21.3   HGNC cytogenetic band: 3q21.3

H1FX Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M130516:     (about GC identifiers)

Start:
130,516,305 bp from pter
End:
130,517,807 bp from pter
Size:
1,503 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000003.10  NT_005612.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: H1X_HUMAN, Q92522 (See protein sequence)
Recommended Name: Histone H1x  
Size: 213 amino acids; 22487 Da
Subcellular location: Nucleus

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006017.1  

    ENSEMBL proteins: 
    ENSP00000329662 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Histone H1.X, Histone H1, Histone H1fx)
    Human Recombinant Proteins from Abnova (H1FX)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000786 nucleosome NAS--
    GO:0005634 nucleus IEA--
    GO:0005694 chromosome IEA--
    About this table

    Antibodies for H1FX: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for H1FX
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Histone H1.X, Histone H1, Histone H1fx), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (H1FX)
    Novus Biologicals Antibody for H1FX

    Assays for H1FX: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR005819 Histone_H5
     IPR011991 Wing_hlx_DNA_bd
     IPR005818 Histone_H1/H5


       GeneDecks  H1FX for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q92522

    ProtoNet protein and cluster: Q92522

    1 Blocks protein family: IPB005818 Histone H1/H5

    UniProtKB/Swiss-Prot: H1X_HUMAN, Q92522
    Similarity: Belongs to the histone H1/H5 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (H1FX)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (H1FX)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006026

                  Applied Biosystems Silencer® siRNAs for H1FX

                  Sigma-Aldrich siRNA for H1FX  
                         Sigma-Aldrich shRNA for H1FX  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for H1FX
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006026
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006026
                                     untagged cDNA clone in CMV expression vector: NM_006026 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006026

    UniProtKB/Swiss-Prot: H1X_HUMAN, Q92522
    Function: Histones H1 are necessary for the condensation of nucleosome chains into higher order
    structures

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding NAS--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    4 Invitrogen iPath™ Online BioAtlas - Pathways for H1FX (Maps provided by GeneGo):
     Cdc25 regulation of cell cycle
     CDC42 in cellular processes
     A2BR signaling via G-alpha-q: PRKCB/G/Q specific pathways (map 3a)
     A2B receptor: action via G-protein alpha s (map 1)

       GeneDecks  H1FX for the pathways selected above  
    About GeneDecksing

    3 Millipore Pathways for H1FX
     Histone modification
     Cell cycle Start of DNA replication in early S phase
     Cell cycle Chromosome condensation in prometaphase

       GeneDecks  H1FX for the pathways selected above  
    About GeneDecksing


    5/6 Interacting proteins for H1FX (Q925222 ENSP000003296623) via UniProtKB, MINT, and/or STRING (see all 6 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DFFBENSP000003674543STRING (score=.899)
    DPM1ENSP000003606403STRING (score=.899)
    ECE1ENSP000003640283STRING (score=.899)
    H1FOOENSP000003197993STRING (score=.899)
    HMGB2ENSP000002965033STRING (score=.899)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006334 nucleosome assembly NAS--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for H1FX

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (H1FX)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (H1FX)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006026

                  Sigma-Aldrich siRNA for H1FX  
                         Sigma-Aldrich shRNA for H1FX  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006026  

    REFSEQ mRNAs for H1FX gene: 

    NM_006026.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006026  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006026
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006026
                                     untagged cDNA clone in CMV expression vector: NM_006026 

    Additional cDNA sequence: 

    BC000426.2 BC010435.1 D64142.1 

    16 DOTS entries:

    DT.87018044  DT.95264824  DT.95163620  DT.120864679  DT.100038883  DT.100795368  DT.100795367  DT.120864671 
    DT.120864663  DT.92467341  DT.101979486  DT.95163621  DT.97860663  DT.100795374  DT.120864662  DT.95264825 

    2 AceView cDNA sequences:

    AI283383 BM728289 

    highest scoring ESTs for H1FX:

    D64142 AJ705273 BF761738 AA236008 AA281262 AA394181 AA424222 AA468585 AA483295 AA515473 

    Unigene Cluster for H1FX:

    H1 histone family, member X
    Hs.75307  [show with all ESTs]
    Unigene Representative Sequence: BC000426


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000333762  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    H1FX expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for H1FX

    1 / 2 / 3

    5 probe-sets matching H1FX gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    319_g_at2, 3 U95-A 1 1.00 1.00 0.92 1.13 D64142 1.00 0.90 0.95 1
    318_at2, 3 U95-A 1 1.00 1.00 0.98 0.80 D64142 1.00 0.90 0.95 1

    64291_at2, 3 U95-C 1 1.00 1.00 0.98 1.06 AI269126 0.40 1.00 0.76 1

    204805_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006026 0.60 1.00 0.82 1

    204805_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  H1FX for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGCCTTTTTT

    SOURCE GeneReport for Unigene cluster: Hs.75307

    Expression variation in blood from EXPOLDB for H1FX

    UniProtKB/Swiss-Prot: H1X_HUMAN, Q92522
    Tissue specificity: Expressed ubiquitously

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for H1FX gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LOC4846271   -- similar to H1 histone family, member X 91.55(n)
    89.2(a)
    484627  XM_541741.2  XP_541741.1 
    chimpanzee
    (Pan troglodytes)
    LOC4709211   -- similar to histone H1x 99.18(n)
    99.02(a)
    470921  XM_526304.2  XP_526304.2 
    cow
    (Bos taurus)
    LOC5119011   -- similar to H1 histone family, member X 89.2(n)
    91.08(a)
    511901  XM_589328.3  XP_589328.3 
    rat
    (Rattus norvegicus)
    H1fx1   -- H1 histone family, member X 80.38(n)
    76.56(a)
    500252  XM_575600.2  XP_575600.1 
    mouse
    (Mus musculus)
    H1fx1, 5 65
    H1 histone family, member X1, 5 82.27(n)1
    80.32(a)1
    2435291  NM_198622.11  NP_941024.11 
     BC0487095 
    About this table        Species with no ortholog for H1FX

    ENSEMBL Gene Tree for H1FX
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for H1FX gene
    H1F02  H1FOO2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/27 NCBI SNPs in H1FX are shown (see all 27 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 22)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 3 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs48091,2
    A,C,F,H130516547(-) AACGGC/TCGTCG 1 -- ut315Minor allele frequency- T:0.08MN EU EA WA 602
    rs48531,2
    A,C,F,H130516426(-) TTCCCA/GGCTCC 1 -- ut314Minor allele frequency- G:0.25EU EA WA 410
    rs40764371,2
    A,C,F,H130518659(+) CTGGGT/CGCACC 1 -- ng314Minor allele frequency- C:0.20EU EA WA 418
    rs40765851,2
    C,F,H130519511(-) GGTCCT/CTCTTC 1 -- ng314Minor allele frequency- C:0.09EU EA WA 418
    rs67959951,2
    A,F,H130517448(+) AGAGGA/GTTGGT 1 -- ut51 tfbs33Minor allele frequency- G:0.28EA WA 294
    rs103451,2
    A,H130516746(-) TTTTTG/TTTTTT 1 -- ut315Minor allele frequency- T:0.00MN EU EA WA 602
    rs40731541,2
    A,F130518175(-) CTGGGC/TTGCCG 1 -- ng314Minor allele frequency- T:0.26NA EA 362
    --
    rs143231,2
    F130517721(-) GCTCGG/ACCTAG 1 -- ut511Minor allele frequency- A:0.17MN 184
    rs84991,2
    A130516456(-) GCGCGC/TGGGGA 1 -- ut310--------
    rs47111,2
    F130516379(-) GCTCCG/CGGCTC 1 -- ut311Minor allele frequency- C:0.01MN 184
    About this table

    HapMap Linkage Disequilibrium images for H1FX (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 602785

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/16 PubMed articles for H1FX gene (see all 16 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 8971 HGNC: 4722 AceView: H1FX Ensembl:ENSG00000184897 euGenes: HUgn8971
    ECgene: H1FX H-InvDB: H1FX
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for H1FX:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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     Predesigned and custom siRNAs for H1FX Antibodies for H1FX
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for H1FX Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
    (Histone H1.X, Histone H1, Histone H1fx)
     Antibodies (Histone H1.X, Histone H1, Histone H1fx)
     Search OriGene for H1FX
     Search Tocris compounds for H1FX




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for H1FX

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