GZMK Gene
protein-coding GIFtS: 52
GCID: GC05P054320
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granzyme K (granzyme 3; tryptase II)(Previous names: granzyme K (serine protease, granzyme 3; tryptase II) )
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Aliases for GZMK gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
|---|
| Granzyme K (Granzyme 3; Tryptase II)1 2 | | Granzyme K2 | | TRYP21 2 3 | | Granzyme-33 | | Granzyme K (Serine Protease, Granzyme 3; Tryptase II)1 2 | | Tryptase II2 | | NK-Tryp-22 3 | | EC 3.4.21.-3 | | NK-Tryptase-21 | | Fragmentin-33 | | PRSS1 | | Granzyme-33 | | Fragmentin-33 | | EC 3.4.218 | | Granzyme 32 | | EC 3.4.21.48 |
Export aliases for GZMK gene to outside databasesPrevious GC identifers: GC05P054483 GC05P055748 GC05P054290 GC05P054335 GC05P054355 GC05P051293 |
Summaries for GZMK gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| Entrez Gene summary for GZMK: This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxiclymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize,bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The proteindescribed here lacks consensus sequences for N-glycosylation present in other granzymes. (provided by RefSeq, Jul2008) Gene Wiki entry for GZMK
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Genomic Views for GZMK gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000005.9 NC_018916.1 NT_006713.15
Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the GZMK gene promoter: Max1 Elk-1 Nkx2-5 NF-kappaB SRY POU2F1 POU2F1a NF-kappaB1 c-Myc Other transcription factors
Search SABiosciences Chromatin IP Primers for GZMK
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GZMK |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 5q11.2 Ensembl cytogenetic band: 5q11.2 HGNC cytogenetic band: 5q11.2GZMK Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 5 GeneLoc Exon Structure GeneLoc location for GC05P054320: view genomic region
(about GC identifiers)
Start:
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54,320,081 bp from pter |
End:
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54,330,398 bp from pter |
Size:
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10,318 bases |
Orientation:
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plus strand |
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Proteins for GZMK gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: GRAK_HUMAN, P49863 (See
protein sequence)Recommended Name: Granzyme K precursor Size: 264 amino acids; 28882 Da
Subcellular location: Secreted. Cytoplasmic granule
2 PDB 3D structures from and Proteopedia for GZMK:1MZA (3D)
  1MZD (3D)
 
Secondary accessions: B2R563Explore the universe of human proteins at neXtProt for GZMK: NX_P49863
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_P49863 4/6 DME Specific Peptides for GZMK (P49863) (see all 6)
GZMK Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_002095.1 ENSEMBL proteins: ENSP00000231009 Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005576 | extracellular region |
IEA | -- |
GZMK for ontologies About GeneDecksing
GZMK Antibody Products: Assay Products for GZMK: |
Protein
Domains / Families for GZMK gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
GZMK for domains About GeneDecksing
4 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry P49863ProtoNet protein and cluster: P49863 2 Blocks protein families: IPB001254 Serine protease IPB001314 Chymotrypsin serine protease family (S1) signature
UniProtKB/Swiss-Prot: GRAK_HUMAN, P49863Similarity: Belongs to the peptidase S1 family. Granzyme subfamilySimilarity: Contains 1 peptidase S1 domain |
Function for GZMK gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Enzyme Numbers (IUBMB): EC 3.4.21.42 EC 3.4.212 EC 3.4.21.-1
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for GZMK (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GZMK OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: GZMK (NM_002104) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for GZMK | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GZMK  |
In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GZMK |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004252 | serine-type endopeptidase activity |
IEA | -- | | GO:0008236 | serine-type peptidase activity |
TAS | 8656064 |
GZMK for ontologies About GeneDecksing
|
Pathways & Interactions for GZMK gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
| Unified GeneCards pathways  About this table  See pathways by source
| Super-pathway | contained gene-specific pathways |
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| 1 | Granzyme Pathway | |
1 Downloadable PowerPoint Slide of QIAGEN Pathway Central Maps for GZMK
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for GZMK
STRING Interaction
Network Preview (showing 2 interactants - click image to see more details)
 5/7 Interacting proteins for GZMK (P498633 ENSP000002310094) via UniProtKB, MINT, STRING, and/or I2D (see all 7)About this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006508 | proteolysis |
IEA | -- |
GZMK for ontologies About GeneDecksing
|
Drugs & Compounds for GZMK gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
GZMK for compounds About GeneDecksing
Browse Tocris compounds for GZMK 3 Novoseek chemical compound relationships for GZMK gene About this table
Search CenterWatch for drugs/clinical trials and news about GZMK / GRAK 
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Transcripts for GZMK gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for GZMK gene: NM_002104.2 Unigene Cluster for GZMK: Granzyme K (granzyme 3; tryptase II) Hs.277937 [show with all ESTs]Unigene Representative Sequence: BM9189272 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000231009(uc003jpl.1) ENST00000512089
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for GZMK (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GZMK OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: GZMK (NM_002104) | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for GZMK | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GZMK  |
Additional cDNA sequence: AK312074.1 BC035802.1 U26174.1 U35237.1 1 DOTS entry: DT.310659 24 AceView cDNA sequences: AA743283 NM_002104 U35237 U26174 AW183169 BC035802 BI908502 CD687238 AU184897 CD702487 AA947915 BX091409 CB987798 BI837702 BM918927 AA005382 AI445141 AA970854 AA699799 CB958972 CD705456 CD702028 AA935709 AA004281 GeneLoc Exon Structure
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Expression for GZMK gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| GZMK expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: --
About this image See GZMK Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for GZMK
SOURCE GeneReport for Unigene cluster: Hs.277937 UniProtKB/Swiss-Prot: GRAK_HUMAN, P49863Tissue specificity: Expressed in lung, spleen, thymus and peripheral blood leukocytes SABiosciences Custom PCR Arrays for GZMK
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for GZMK Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat GZMK | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat GZMK | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GZMK | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GZMK |
Orthologs for GZMK gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for GZMK gene from 3/14 species (see all 14) About this table
ENSEMBL Gene Tree for GZMK (if available) TreeFam Gene Tree for GZMK (if available)  |
Paralogs for GZMK gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| Paralogs for GZMK gene
- PRTN32 GZMH2 PRSS572 CTSG2 CFD2 ELANE2 CMA12 AZU12
- GZMM2 GZMB2 GZMA2
18/103 SIMAP similar genes for GZMK using alignment to 1 protein entry: GRAK_HUMAN(see all similar genes):GZMA CFD DF GZMH TMPRSS15 KLK9 CELA1 KLK11 KLK14 KLK8 PLAU CTRL CTSG GZMM KLK4 PRSS2 TMPRSS11F TRY8
GZMK for paralogs About GeneDecksing
|
Genomic Variants for GZMK gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 5 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
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HapMap Linkage Disequilibrium report for GZMK (54320081 - 54330398 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV) variations for GZMK: --  | SABiosciences Cancer Mutation PCR Assays |
|  | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing GZMK |
|
Disorders
/ Diseases for GZMK gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
GZMK for disorders About GeneDecksing
OMIM gene information: 600784
OMIM disorders: --
13 diseases for GZMK: About MalaCardslarge granular lymphocyte leukemia t-cell large granular lymphocyte leukemia pityriasis rosea arts syndrome epicondylitis lymphocytic leukemia acute lymphoblastic leukemia lymphoblastic leukemia allergic asthma multiple sclerosis leukemia asthma melanoma 1 disease from the University of Copenhagen DISEASES database for GZMK:Arts syndrome 2 Novoseek disease relationships for GZMK gene About this table
| Disease |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| melanoma |
13.5 |
1 |
16596996 (1) |
| tumors |
0 |
1 |
19201868 (1) |
Export disorders for GZMK gene to outside databases
|
Publications for GZMK gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for GZMK gene, integrated from 9 sources (see all 44): (articles sorted by number of sources associating them with GZMK) | |  | Utopia: connect your pdf to the dynamic world of online information |
- Cloning of cDNA for human granzyme 3. (PubMed id 7758581)1, 2, 3, 9 Przetak M.M.... Schmidt B.F. (1995)
- The 2.2-A crystal structure of human pro-granzyme K reveals a rigid zymogen with unusual features. (PubMed id 12384499)1, 2, 9 Hink-Schauer C.... Jenne D.E. (2002)
- Cloning and expression of a second human natural killer cell granule tryptase, HNK-Tryp-2/granzyme 3. (PubMed id 8656064)1, 2, 9 Sayers T.J.... Smyth M.J. (1996)
- Purification of three cytotoxic lymphocyte granule serine proteases that induce apoptosis through distinct substrate and target cell interactions. (PubMed id 1460416)1, 2, 9 Shi L.... Greenberg A.H. (1992)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Characterization of three serine esterases isolated from human IL-2 activated killer cells. (PubMed id 3262682)1, 2 Hameed A.... Podack E.R. (1988)
- Ignition of p53 bomb sensitizes tumor cells to granzyme K-mediated cytolysis. (PubMed id 19201868)1, 9 Hua G....Fan Z. (2009)
- Granzyme K directly processes bid to release cytochrome c and endonuclease G leading to mitochondria-dependent cell death. (PubMed id 17308307)1, 9 Zhao T....Fan Z. (2007)
- Altered levels and molecular forms of granzyme k in plasma from septic patients. (PubMed id 17438453)1, 9 Rucevic M....Lim Y.P. (2007)
- Granzyme K displays highly restricted substrate specificity that only partially overlaps with granzyme A. (PubMed id 19059912)1, 9 Bovenschen N....Kummer J.A. (2009)
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External Searches for GZMK gene
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
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Genome Databases showing GZMK gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing GZMK gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing GZMK gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| Name | Description |
| PharmGKB entry for GZMK | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for GZMK gene: Search GeneIP for patents involving GZMK
GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products for GZMK gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
|
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 | |
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| | | | Browse OriGene Antibodies | | OriGene shRNA RFP for GZMK | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for GZMK | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for GZMK | | OriGene Protein Over-expression Lysate for GZMK | | Browse OriGene Fluorogenic Cell Assay Kits | | OriGene siRNA for GZMK | | OriGene 3'-UTR Clone for GZMK | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GZMK | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for GZMK | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | OriGene Custom Antibody Services for GZMK | | OriGene Custom Protein Services for GZMK | | OriGene Custom Immunoassay Development | | |
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| | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat GZMK | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing GZMK | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GZMK | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat GZMK | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GZMK | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat GZMK |
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| | | Search Tocris compounds for GZMK |
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 | | GZMK Proteins, Antibodies, CLIAs, and ELISAs |
| | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GZMK |
|  |  |  | | | ThermoFisher Antibody for GZMK |
| | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GZMK |
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