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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

GSX2 Gene

protein-coding   GIFtS: 43
GCID: GC04P054966

GS homeobox 2

 Explore 3 diseases affiliated with
GSX2 via our new
 Human Malady Compendium 
Biological research products
for GSX2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
GS Homeobox 21 2
Homeobox Protein GSH-22 3
GSH22 3
Gsh21

External Ids:    HGNC: 249591   Entrez Gene: 1708252   Ensembl: ENSG000001806137   UniProtKB: Q9BZM33   

Export aliases for GSX2 gene to outside databases

Previous GC identifers: GC04P054661 GC04P050913


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: GSX2_HUMAN, Q9BZM3
Function: Probable transcription factor that binds to the DNA sequence 5'-CNAATTAG-3' (By similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000004.11  NC_018915.1  NT_022853.15  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the GSX2 gene promoter:
         AREB6   PPAR-gamma1   POU2F1   POU2F1a   Nkx2-2   AP-2beta   ZID   PPAR-gamma2   AP-2gamma   AP-2alphaA   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidGSX2 promoter sequence
   Search SABiosciences Chromatin IP Primers for GSX2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GSX2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q12   Ensembl cytogenetic band:  4q12   HGNC cytogenetic band: 4q12

GSX2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GSX2 gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P054966:  view genomic region     (about GC identifiers)

Start:
54,965,690 bp from pter      End:
54,968,672 bp from pter
Size:
2,983 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: GSX2_HUMAN, Q9BZM3 (See protein sequence)
Recommended Name: GS homeobox 2  
Size: 304 amino acids; 32031 Da
Subcellular location: Nucleus (By similarity)

Explore the universe of human proteins at neXtProt for GSX2: NX_Q9BZM3

GSX2 Protein expression data from MOPED and PaxDb:    About this image 

Estimated protein expression log10 (pmol).

REFSEQ proteins: NP_573574.1  
ENSEMBL proteins: 
 ENSP00000319118   ENSP00000422213  

Human Recombinant Protein Products: 
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Uscn Proteins for GSX2

Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005634nucleus IEA--


GSX2 for ontologies           About GeneDecksing



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Uscn ELISAs and CLIAs for GSX2


(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

GSX2 for domains           About GeneDecksing

4 InterPro domains/families:
 IPR020479 Homeobox_metazoa
 IPR017970 Homeobox_CS
 IPR001356 Homeodomain
 IPR009057 Homeodomain-like

Graphical View of Domain Structure for InterPro Entry Q9BZM3

ProtoNet protein and cluster: Q9BZM3

1 Blocks protein family: IPB000047 Lambda and other repressor helix-turn-helix signature

UniProtKB/Swiss-Prot: GSX2_HUMAN, Q9BZM3
Similarity: Belongs to the Antp homeobox family
Similarity: Contains 1 homeobox DNA-binding domain


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

     UniProtKB/Swiss-Prot: GSX2_HUMAN, Q9BZM3
Function: Probable transcription factor that binds to the DNA sequence 5'-CNAATTAG-3' (By similarity)

miRNA
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Inhib. RNA
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Gene Editing
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In Situ Assay
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Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GSX2

Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0003677DNA binding ----
GO:0003700sequence-specific DNA binding transcription factor activity IEA--
GO:0043565sequence-specific DNA binding IEA--


GSX2 for ontologies           About GeneDecksing


Animal Models:
     Mouse knock-outs for GSX2: Gsx2tm1Ssp Gsx2tm2.2Kc
     8 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Gsx2):
 behavior/neurological  cellular  endocrine/exocrine gland  homeostasis/metabolism  mortality/aging 
 nervous system  normal  respiratory system 

GSX2 for phenotypes           About GeneDecksing


(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section



Interactions:

    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for GSX2

STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

2 Interacting proteins for GSX2 (Q9BZM33 ENSP000003191184) via UniProtKB, MINT, STRING, and/or I2D
InteractantInteraction Details
GeneCardExternal ID(s)
ABL1P005193, ENSP000003614234I2D: score=1 STRING: ENSP00000361423
NKX2-1P436993I2D: score=1 
About this table

Gene Ontology (GO): 5/14 biological process terms (GO ID links to tree view) (see all 14):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0002087regulation of respiratory gaseous exchange by neurological system process IEA--
GO:0006351transcription, DNA-dependent IEA--
GO:0007275multicellular organismal development ----
GO:0007389pattern specification process ----
GO:0007417central nervous system development ----


GSX2 for ontologies           About GeneDecksing



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section
Browse Small Molecules at EMD Millipore
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for GSX2
Search CenterWatch for drugs/clinical trials and news about GSX2 

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for GSX2 gene: 
NM_133267.2  

Unigene Cluster for GSX2:

GS homeobox 2
Hs.371899  [show with all ESTs]
Unigene Representative Sequence: AB028838
4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000507839 ENST00000326902(uc010igp.1) ENST00000503800 ENST00000548609


miRNA
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Inhib. RNA
Products:
     
Browse for Gene Knock-down Tools from EMD Millipore
OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for GSX2 (see all 7)
OriGene shRNA RFP: GSX2
OriGene siRNA: GSX2
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat GSX2
Clone
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Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GSX2 
Primer
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  QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat GSX2
  QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GSX2

Additional cDNA sequence: 

AB028838.1 BC075089.2 BC075090.2 EU596451.1 

2 DOTS entries:

DT.109238  DT.40295422 

24/36 AceView cDNA sequences (see all 36):

BX113485 AI360912 CB054287 AA485025 AI357521 AA744807 AI356125 NM_133267 
BX096763 BC075089 CB054288 BE551998 BF508282 AB028838 AI971447 AA485198 
AI678083 AW204644 AI422187 AA485095 BC075090 AI937017 AI934486 AI797226 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

GSX2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TACCTGTCTC

Microarray
RNAseq (Illumina Body Map)
(100×FPKM)½
SAGE (Serial Analysis of Gene Expression)

About this image

GSX2 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
4 LifeMap In Vivo Development Anatomical Compartments/Cells 
Tissue Anatomical Compartment CellCategory (developmental path)
BrainCerebral CortexBrain
BrainThalamusBrain
Neural TubeDiencephalic Ventricular ZoneNeural Tube
Neural TubeTelencephalonNeural Tube
Expression: Positive    Negative     Selective marker
Experimental details: Curated     Microarrays     In-situ hybridization
Stem Cell Differentiation: 1 LifeMap Cell 
NameCategory
Lateral Ganglionic Eminence progenitors (Generation of forebr...)

See GSX2 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for GSX2

SOURCE GeneReport for Unigene cluster: Hs.371899
    SABiosciences Custom PCR Arrays for GSX2
Primer
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QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GSX2
In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GSX2

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for GSX2 gene from 4/14 species (see all 14)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
chicken
(Gallus gallus)
Aves LOC4299941 GS homeobox 2-like 76.86(n)
79.62(a)
  429994  XM_003641207.1  XP_003641255.1 
lizard
(Anolis carolinensis)
Reptilia GSX26
--
62(a)
1 ↔ 1
5(103977912-103983837)
zebrafish
(Danio rerio)
Actinopterygii gsx21 GS homeobox 2 70.26(n)
74.69(a)
  561076  NM_001025512.2  NP_001020683.1 
fruit fly
(Drosophila melanogaster)
Insecta ind6
intermedia...
24(a)
1 → many
3L(15035894-15037024)


ENSEMBL Gene Tree for GSX2 (if available)
TreeFam Gene Tree for GSX2 (if available) 

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
Paralogs for GSX2 gene
GSX12  HOXA72  HOXA12  HOXD12  HOXA22  HOXB32  HOXC62  HOXB52  
HOXC52  HOXB42  HOXA52  HOXA42  HOXD32  HOXA62  HOXC82  HOXA32  
HOXD82  HOXB12  HOXB62  HOXB82  HOXD42  HOXB22  HOXB72  PDX12  
HOXC42  
7 SIMAP similar genes for GSX2 using alignment to 3 protein entries:     GSX2_HUMAN (see all proteins):
HOXC13    HOX B6    MEOX1    HOXA4    HOXB1    NKX2-5
GSX1

GSX2 for paralogs           About GeneDecksing



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
SABiosciences Cancer Mutation PCR Assays
QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing GSX2
DNA2.0 Custom Variant and Variant Library Synthesis for GSX2

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

GSX2 for disorders           About GeneDecksing

3 diseases for GSX2:    About MalaCards
myeloid leukemia    leukemia    neuronitis


Export disorders for GSX2 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
About This Section

PubMed articles for GSX2 gene, integrated from 9 sources (see all 14):
(articles sorted by number of sources associating them with GSX2)
    Utopia: connect your pdf to the dynamic
world of online information

  1. Evidence for position effects as a variant ETV6-mediated leukemogenic mechanism in myeloid leukemias with a t(4;12)(q11-q12;p13) or t(5;12)(q31;p13). (PubMed id 11861295)1, 3, 9 Cools J....Marynen P. (2002)
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
  3. Heterozygous truncating mutation in the human homeobox gene GSH2 has no discernable phenotypic effect. (PubMed id 12205114)1, 3 Dauwerse J.G....Peters D.J. (2002)
  4. The homeobox gene Gsx2 regulates the self-renewal and differentiation of neural stem cells and the cell fate of postnatal progenitors. (PubMed id 22242181)1 Mendez-Gomez H.R. and Vicario-Abejon C. (2012)
  5. MicroRNA-9 regulates neurogenesis in mouse telencepha lon by targeting multiple transcription factors. (PubMed id 21368052)1 Shibata M....Aizawa S. (2011)
  6. Distinct temporal requirements for the homeobox gene Gsx2 in specifying striatal and olfactory bulb neuronal fates. (PubMed id 19709628)1 Waclaw R.R....Campbell K. (2009)
  7. Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening. (PubMed id 17474147)1 Wu C....Li S.S. (2007)
  8. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)2 Hillier L.W....Wilson R.K. (2005)
  9. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
  10. Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )2 Aoki H.... Hino O. (2000)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 170825 HGNC: 24959 AceView: GSH-2 Ensembl:ENSG00000180613 euGenes: HUgn170825
ECgene: GSX2 H-InvDB: GSX2

(According to HUGE)
About This Section
  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
NameDescription
PharmGKB entry for GSX2 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
About This Section
Patent Information for GSX2 gene:
Search GeneIP for patents involving GSX2

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section

 Browse Kits and Assays available from EMD Millipore
 EMD Millipore Mono- and Polyclonal Antibodies for the study of GSX2
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GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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VWF
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von Willebrand factor
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