Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 
or upload a file of gene symbols


Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

GRK5 Gene

protein-coding   GIFtS: 66
GCID: GC10P120957

G protein-coupled receptor kinase 5


(Previous symbol: GPRK5)
 Explore 11 diseases affiliated with
GRK5 via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for GRK5    

Aliases
for GRK5 gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
G Protein-Coupled Receptor Kinase 51 2
GPRK51 2 3 5
G Protein-Coupled Receptor Kinase GRK52 3
EC 2.7.11.163 8
FP20252
EC 2.7.118

External Ids:    HGNC: 45441   Entrez Gene: 28692   Ensembl: ENSG000001988737   OMIM: 6008705   UniProtKB: P349473   

Export aliases for GRK5 gene to outside databases

Previous GC identifers: GC10P120631 GC10P114596


Summaries
for GRK5 gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
Entrez Gene summary for GRK5:
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of
the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus
initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear
leukocytes (PMNs). (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: GRK5_HUMAN, P34947
Function: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of
G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor
desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of
GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4
subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates:
Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits
G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of
myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription.
Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13
regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non
G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro)

Gene Wiki entry for GRK5


Genomic Views
for GRK5 gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000010.10  NC_018921.1  NT_030059.13  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the GRK5 gene promoter:
         AML1a   AP-2gamma   Gfi-1   Meis-1b   YY1   CREB   deltaCREB   IRF-7A   Meis-1a   Meis-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: GRK5 promoter sequence
   Search SABiosciences Chromatin IP Primers for GRK5

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GRK5


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q26.11   Ensembl cytogenetic band:  10q26.11   HGNC cytogenetic band: 10q24-qter

GRK5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GRK5 gene location

GeneLoc information about chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P120957:  view genomic region     (about GC identifiers)

Start:
 120,967,101 bp from pter      End:
 121,215,131 bp from pter
Size:
 248,031 bases      Orientation:
 plus strand

Proteins
for GRK5 gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: GRK5_HUMAN, P34947 (See protein sequence)
Recommended Name: G protein-coupled receptor kinase 5  
Size: 590 amino acids; 67787 Da
Subunit: Interacts with ST13 (via the C-terminus 303-319 AA). Interacts with TP53/p53. Interacts with HTR4 (via
C-terminus 330-346 AA); this interaction is promoted by 5-HT (serotonin). Interacts with HDAC5 (By similarity)
Subcellular location: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Note=Predominantly localized at
the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is
regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion
Secondary accessions: D3DRD0 Q5T059

Explore the universe of human proteins at neXtProt for GRK5: NX_P34947

Post-translational modifications:

  • Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_P34947

    • 4/26 DME Specific Peptides for GRK5 (P34947) (see all 26)
     MNGGDLK  LNEKQIL  PPFVPDP  LQWKWLE 

    GRK5 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_005299.1  
    ENSEMBL proteins: 
     ENSP00000358102   ENSP00000376609   ENSP00000358104  
    Reactome Protein details: P34947
    Human Recombinant Protein Products: 
    EMD Millipore Purified and/or Recombinant GRK5 Protein
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for GRK5
    OriGene Purified Protein: GRK5
    OriGene Protein Over-expression Lysate: GRK5
    OriGene Custom Protein Services for GRK5 
    GenScript Custom Purified and Recombinant Proteins Services for GRK5
    Novus Biologicals GRK5 Proteins
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for GRK5

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005625soluble fraction ----
    GO:0005634nucleus IEA--
    GO:0005737cytoplasm IEA--
    GO:0005886plasma membrane IDA17986524


    GRK5 for ontologies           About GeneDecksing



    GRK5 Antibody Products: 
    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    R&D Systems Antibodies for GRK5
    OriGene Antibodies: GRK5
    OriGene Custom Antibody Services for GRK5 
    GenScript Custom Superior Antibodies Services for GRK5
    Novus Biologicals GRK5 Antibodies
    Abcam antibodies for GRK5 
    Uscn Antibodies for GRK5
    ThermoFisher Antibody for GRK5

    Assay Products for GRK5: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Immunoassay Development
    Browse OriGene Fluorogenic Cell Assay Kits
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for GRK5
    Browse Enzo Life Sciences for kits & assays
    Uscn ELISAs and CLIAs for GRK5

    Protein Domains /
    Families
    for GRK5 gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    GRK5 for domains           About GeneDecksing

    5/8 InterPro domains/families (see all 8):
     IPR017441 Protein_kinase_ATP_BS
     IPR000239 GPCR_kinase
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR011009 Kinase-like_dom
     IPR000719 Prot_kinase_cat_dom

    Graphical View of Domain Structure for InterPro Entry P34947

    ProtoNet protein and cluster: P34947

    3 Blocks protein families:
    IPB000239 GPCR kinase signature
    IPB000342 Regulator of G-protein signalling (RGS) protein signature
    IPB000961 Protein kinase C-terminal domain


    UniProtKB/Swiss-Prot: GRK5_HUMAN, P34947
    Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily
    Similarity: Contains 1 AGC-kinase C-terminal domain
    Similarity: Contains 1 protein kinase domain
    Similarity: Contains 1 RGS domain


    Function
    for GRK5 gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: GRK5_HUMAN, P34947
    Function: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of
    G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor
    desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of
    GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4
    subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates:
    Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits
    G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of
    myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription.
    Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13
    regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non
    G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro)
    Catalytic activity: ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate
    Enzyme regulation: Inhibited by calmodulin with an IC(50) of 50 nM. Calmodulin inhibits GRK5 association with receptor
    and phospholipid
    Induction: Overexpressed during heart failure

    Enzyme Numbers (IUBMB): EC 2.7.11.161 2 EC 2.7.112

    miRNA
    Products:            		 OriGene 3'-UTR Clone: GRK5
    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat GRK5
    8 QIAGEN miScript miRNA Assays for microRNAs that regulate GRK5:
    hsa-miR-539 hsa-miR-106a* hsa-miR-1304 hsa-miR-3168 hsa-miR-135b hsa-miR-1299 hsa-miR-4277 hsa-miR-135a
    SwitchGear 3'UTR luciferase reporter plasmid: GRK5 3' UTR sequence
    Inhib. RNA
    Products:            		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for GRK5 (see all 4)
    OriGene shRNA RFP: GRK5
    OriGene siRNA: GRK5
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat GRK5

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for GRK5

    Clone
    Products:             		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for GRK5 (see all 3)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GRK5 (see all 2)
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector: GRK5 (NM_005308)
    Sino Biological Human cDNA Clone for GRK5
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for GRK5
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GRK5 

    Cell Line
    Products:             		GenScript Custom overexpressing Cell Line Services for GRK5
    Search LifeMap BioReagents cell lines for GRK5

    In Situ Assay
    Products:       
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GRK5

    Gene Ontology (GO): 5/8 molecular function terms (GO ID links to tree view) (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004674protein serine/threonine kinase activity IDA--
    GO:0004703G-protein coupled receptor kinase activity IEA--
    GO:0004872receptor activity ----
    GO:0005080protein kinase C binding TAS9813065
    GO:0005515protein binding IPI17986524


    GRK5 for ontologies           About GeneDecksing


    3 GenomeRNAi human phenotypes for GRK5:
     Decreased Wnt reporter activit  Increased cell death HMECs cel  Increased mitotic index 

    Animal Models:
         Mouse knock-out Grk5tm1Rjl for GRK5
         9 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Grk5):
     behavior/neurological  digestive/alimentary  endocrine/exocrine gland  homeostasis/metabolism  integument 
     muscle  nervous system  normal  respiratory system 

    GRK5 for phenotypes           About GeneDecksing


    Pathways & Interactions
    for GRK5 gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/8 super-pathways (see all 8) About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Signaling by GPCR
    		Signaling by GPCR1.00
    		Signal Transduction0.56
    		GPCR downstream signaling0.89
    2G alpha (q) signalling events
    		G alpha (q) signalling events1.00
    		Gastrin-CREB signalling pathway via PKC and MAPK0.90
    3Development Angiotensin signaling via beta-Arrestin
    		Development Angiotensin signaling via beta-Arrestin1.00
    		Development_Angiotensin signaling via beta-Arrestin0.96
    4Calcium Regulation in the Cardiac Cell
    		Calcium Regulation in the Cardiac Cell1.00
    		Myometrial Relaxation and Contraction Pathways0.43
    5Chemokine Signaling
    		Chemokine Signaling1.00
    		Chemokine signaling pathway0.33

    Pathway sources
    See GeneCards unified pathways
    Show all pathways

    1 EMD Millipore Pathway for GRK5
        Development Angiotensin signaling via beta-Arrestin

    2 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for GRK5
        Chemokine Signaling
    Colorectal Cancer Metastasis

    1 GeneGo (Thomson Reuters) Pathway for GRK5
        Development Angiotensin signaling via beta-Arrestin

    3 BioSystems Pathways for GRK5 
        Calcium Regulation in the Cardiac Cell
    Myometrial Relaxation and Contraction Pathways
    Alpha-synuclein signaling

    5        Reactome Pathways for GRK5
        GPCR downstream signaling
    Gastrin-CREB signalling pathway via PKC and MAPK
    Signal Transduction
    G alpha (q) signalling events
    Signaling by GPCR


    2         Kegg Pathways  (Kegg details for GRK5):
        Chemokine signaling pathway
    Endocytosis


    GRK5 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for GRK5

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/157 Interacting proteins for GRK5 (P349472, 3 ENSP000003766094) via UniProtKB, MINT, STRING, and/or I2D (see all 157)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ARRB1P494072, ENSP000003771414MINT-7943519 MINT-7943553 MINT-7943535 MINT-7943476 MINT-7943501 STRING: ENSP00000377141
    CALM1P621583, ENSP000003494674I2D: score=1 STRING: ENSP00000349467
    CALM2P621583I2D: score=1 
    CALM3P621583I2D: score=1 
    CAV1Q031353, ENSP000003391914I2D: score=3 STRING: ENSP00000339191
    About this table

    Gene Ontology (GO): 5/10 biological process terms (GO ID links to tree view) (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006915apoptotic process IEA--
    GO:0007165signal transduction ----
    GO:0007188adenylate cyclase-modulating G-protein coupled receptor signaling pathway TAS8626574
    GO:0007217tachykinin receptor signaling pathway IDA17986524
    GO:0008277regulation of G-protein coupled receptor protein signaling pathway TAS8288567


    GRK5 for ontologies           About GeneDecksing



    Drugs & Compounds
    for GRK5 gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    GRK5 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for GRK5
    10/15 Novoseek chemical compound relationships for GRK5 gene (see all 15)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    pip2 48.6 6 8798768 (4), 10818226 (1)
    threonine 46.5 1 19661922 (1)
    serine 37.6 1 19661922 (1)
    dextran sulfate 31.4 3 8288567 (1), 8077221 (1)
    isoproterenol 28.5 1 18034461 (1)
    leukotriene b4 17 1 12077128 (1)
    phospholipid 10.2 7 8144599 (3), 9218466 (1), 9813065 (1)
    phosphoinositide 6.89 1 10861009 (1)
    atp 0 1 8288567 (1)
    sucrose 0 1 8170959 (1)

    1 PharmGKB related drug/compound annotation for GRK5 gene
    Drug/compound PharmGKB Annotation
    Beta Blocking AgentsCA  
    About this table

    Search CenterWatch for drugs/clinical trials and news about GRK5 

    Transcripts
    for GRK5 gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for GRK5 gene: 
    NM_005308.2  

    Unigene Clusters for GRK5:

    G protein-coupled receptor kinase 5
    Hs.524625  [show with all ESTs], Hs.736460  [show with all ESTs]
    Unigene Representative Sequences: NM_005308, AK097099
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000369106 ENST00000392870(uc001led.3 uc009xzh.3 uc010qta.1)
    ENST00000473264 ENST00000369108

    miRNA
    Products:             		 OriGene 3'-UTR Clone: GRK5
    Browse OriGene MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat GRK5
    8 QIAGEN miScript miRNA Assays for microRNAs that regulate GRK5:
    hsa-miR-539 hsa-miR-106a* hsa-miR-1304 hsa-miR-3168 hsa-miR-135b hsa-miR-1299 hsa-miR-4277 hsa-miR-135a
    SwitchGear 3'UTR luciferase reporter plasmid: GRK5 3' UTR sequence
    Inhib. RNA
    Products:             		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for GRK5 (see all 4)
    OriGene shRNA RFP: GRK5
    OriGene siRNA: GRK5
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat GRK5
    Clone
    Products:             		 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for GRK5 (see all 3)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GRK5 (see all 2)
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector: GRK5 (NM_005308)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for GRK5
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GRK5 
    Primer
    Products:            		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for GRK5
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat GRK5
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat GRK5
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GRK5

    Additional cDNA sequence: AK097099.1 

    9 DOTS entries:

    DT.40110887  DT.102819582  DT.92010080  DT.95370282  DT.91640334  DT.121250959  DT.102833238  DT.95204296 
    DT.97844869 

    24/153 AceView cDNA sequences (see all 153):

    BQ066445 AI358974 BF195014 AA059451 AW206339 AI200481 BU146436 BU526699 
    W77864 BQ069941 BQ918795 BE328060 BQ894797 BQ061150 BM849661 BU681200 
    BM719846 BQ071007 AA059452 BP345276 BQ061253 BQ065277 BM709198 AA121357 

    GeneLoc Exon Structure


    Expression
    for GRK5 gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    GRK5 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CACGTCCTCC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    GRK5 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 3 LifeMap Cells 
    NameCategory
    PureStem™ mesenchymal progenitor 7PEND24 (Embryonic Progenitor Cell)Cartilage, Neural Crest
    PureStem™ progenitor EN27 (Embryonic Progenitor Cell)
    Posterior foregut-like cells (A scalable, suspensi...)
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See GRK5 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for GRK5

    SOURCE GeneReport for Unigene clusters: Hs.524625 Hs.736460

    UniProtKB/Swiss-Prot: GRK5_HUMAN, P34947
    Tissue specificity: Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney

        SABiosciences Custom PCR Arrays for GRK5
    Primer
    Products:    		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for GRK5
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat GRK5
    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat GRK5
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GRK5
    In Situ
    Assay Products:     
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GRK5

    Orthologs
    for GRK5 gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of eukaryotes.

    Orthologs for GRK5 gene from 6/23 species (see all 23)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    chicken
    (Gallus gallus)    		 Aves GRK51 G protein-coupled receptor kinase 5 80.45(n)
    91.02(a)    		   423929  XM_421789.2  XP_421789.2 
    lizard
    (Anolis carolinensis)    		 Reptilia GRK56
    --
    		 --
    		 89(a)
    72(a)
    		 1 ↔ 1
    possible ortholog
    		 GL343399.1(818907-882165)
    GL343406.1(705345-734627)
    zebrafish
    (Danio rerio)    		 Actinopterygii BQ419577.12   -- 75.25(n)    BQ419577.1 
    fruit fly
    (Drosophila melanogaster)    		 Insecta Gprk21 G protein-coupled receptor kinase 2 63.16(n)
    63.77(a)    		   49045  NM_057519.3  NP_476867.1 
    worm
    (Caenorhabditis elegans)    		 Secernentea grk-11 Protein GRK-1 57.77(n)
    59.86(a)    		   181212  NM_077275.3  NP_509676.1 
    rice
    (Oryza sativa)    		 Liliopsida Os.475052 Oryza sativa (japonica cultivar-group) cDNA cloneJ more 75.01(n)    AK065005.1 


    ENSEMBL Gene Tree for GRK5 (if available)
    TreeFam Gene Tree for GRK5 (if available) 

    Paralogs
    for GRK5 gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for GRK5 gene
    GRK72  ADRBK22  GRK12  ADRBK12  GRK42  GRK62  
    18/58 SIMAP similar genes for GRK5 using alignment to 4 protein entries:     GRK5_HUMAN (see all proteins) (see all similar genes):
    GRK6    GRK4    GRK1    GRK7    MAP3K11    PRKD3
    MAPK8    MARK4    PRKCH    PRKCZ    RPS6KA5    ADRBK1
    CDK7    PDPK2    PRKY    PkB-like 1    SGK3    ADRBK2

    GRK5 for paralogs           About GeneDecksing



    Genomic Variants
    for GRK5 gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    UniProtKB/Swiss-Prot: GRK5_HUMAN, P34947
    Polymorphism: Variant Leu-41 variant is rare in European-Americans individuals but common in African-Americans
    individuals (40% of the African-American individuals studied carry at least one allele). Variant leu-41 is associated
    with decreased mortality in African-Americans with heart failure or cardiac ischemia


    10/4465 NCBI SNPs in GRK5 are shown (see all 4465)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 10 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs1412672351,2
    		--120965255(+) CCCGGC/GCCAAG 1 -- us2k10--------
    rs1822881861,2
    		--120965296(+) GGCATA/TCAAAA 1 -- us2k10--------
    rs1392221281,2
    		C,--120965304(+) AAAAT-/TTTC  
                TTTCT     		1 -- us2k10--------
    rs2009668501,2
    		--120965309(+) TTTCT-/TTCTTTTT 1 -- us2k10--------
    rs2016061851,2
    		--120965311(+) TCTTT-/CTTTTT 1 -- us2k10--------
    rs111988211,2
    		C,F,--120965341(+) NNNNAA/GACTTC 1 -- us2k15Minor allele frequency- G:0.11WA CSA NA EA 362
    rs795243471,2
    		C,F,--120965385(+) ACCAGC/TGTGGT 1 -- us2k11Minor allele frequency- T:0.07EA 120
    rs1869072311,2
    		--120965615(+) GATAAA/GTGAGG 1 -- us2k10--------
    rs801179721,2
    		F,--120965616(+) ATAAAT/CGAGGC 1 -- us2k11Minor allele frequency- C:0.07EA 120
    rs765870771,2
    		F,--120965821(+) GACTTC/GCTGGC 1 -- us2k11Minor allele frequency- G:0.07EA 120

    HapMap Linkage Disequilibrium report for GRK5 (120967101 - 121215131 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 3 variations for GRK5
         1 CNV: 101058
         2 Indels: 61067 41828
    Human Gene Mutation Database (HGMD): GRK5

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing GRK5
    DNA2.0 Custom Variant and Variant Library Synthesis for GRK5

    Disorders / Diseases
    for GRK5 gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    GRK5 for disorders           About GeneDecksing

    OMIM gene information: 600870    OMIM disorders: --

    11 diseases for GRK5:    About MalaCards
    anorexia nervosa    neurodegenerative disease    huntington's disease    rheumatoid arthritis
    parkinson's disease    leiomyoma    alzheimer's disease    colon cancer
    arthritis    sepsis    colorectal cancer

    4 Novoseek disease relationships for GRK5 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    heart failure 33.8 2 20443857 (1), 18425130 (1)
    heart failure congestive 12.1 1 10448093 (1)
    huntington disease 9.95 1 8415712 (1)
    neurodegenerative diseases 0 1 17146290 (1)

    Human Genome Epidemiology (HuGE) Navigator: GRK5 (17 documents)

    Export disorders for GRK5 gene to outside databases

    Publications
    for GRK5 gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for GRK5 gene, integrated from 9 sources (see all 159):
    (articles sorted by number of sources associating them with GRK5)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. Cloning and expression of GRK5: a member of the G protein-coupled receptor kinase family. (PubMed id 7685906)1, 2, 3, 9 Kunapuli P. and Benovic J.L. (1993)
    2. G protein-coupled receptor kinase 5 contains a DNA-binding nuclear localization sequence. (PubMed id 15542828)1, 2, 9 Johnson L.R....Pitcher J.A. (2004)
    3. A predicted amphipathic helix mediates plasma membrane localization of GRK5. (PubMed id 14976207)1, 2, 9 Thiyagarajan M.M....Wedegaertner P.B. (2004)
    4. Phospholipid-stimulated autophosphorylation activates the G protein-coupled receptor kinase GRK5. (PubMed id 8144599)1, 2, 9 Kunapuli P....Benovic J.L. (1994)
    5. Role for the regulator of G-protein signaling homolog y domain of G protein-coupled receptor kinases 5 and 6 in beta 2-adrenergic rec eptor and rhodopsin phosphorylation. (PubMed id 20038610)1, 2, 9 Baameur F....Clark R.B. (2010)
    6. Beta-arrestin1 phosphorylation by GRK5 regulates G pr otein-independent 5-HT4 receptor signalling. (PubMed id 19661922)1, 2, 9 Barthet G....Dumuis A. (2009)
    7. A GRK5 polymorphism that inhibits beta-adrenergic receptor signaling is protective in heart failure. (PubMed id 18425130)1, 2, 9 Liggett S.B....Dorn G.W. (2008)
    8. G protein-coupled receptor kinase 5 phosphorylation of Hip regulates internalization of the chemokine receptor CXCR4. (PubMed id 21728385)1, 2 Barker B.L. and Benovic J.L. (2011)
    9. G-protein-coupled receptor kinase 5 phosphorylates p5 3 and inhibits DNA damage-induced apoptosis. (PubMed id 20124405)1, 2 Chen X....Ma L. (2010)
    10. G Protein-coupled receptor kinases phosphorylate LRP6 in the Wnt pathway. (PubMed id 19801552)1, 2 Chen M....Chen W. (2009)

    External Searches for GRK5 gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing GRK5 gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 2869 HGNC: 4544 AceView: GRK5 Ensembl:ENSG00000198873 euGenes: HUgn2869
    ECgene: GRK5 Kegg: 2869 H-InvDB: GRK5

    Other Databases showing GRK5 gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing GRK5 gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for GRK5 Pharmacogenomics, SNPs, Pathways

    Intellectual Property
    for GRK5 gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for GRK5 gene:
    Search GeneIP for patents involving GRK5

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    Products
    for GRK5 gene

    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
    About This Section

     Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
     Browse for Gene Knock-down Tools from EMD Millipore
     EMD Millipore Purified and/or Recombinant GRK5 Protein
     Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
     Browse Kits and Assays available from EMD Millipore
     Browse Small Molecules at EMD Millipore
     
     EMD Millipore Custom Antibody & Bulk Services
     EMD Millipore Preclinical / Clinical Development Services
     EMD Millipore Immunoassay Services
     EMD Millipore Target Screening & Profiling Services

      
     Antibodies for GRK5 (GRK5)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Kinase Activity Assays/Reagents  
     Browse ELISpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Multiplex/Array Assay Kits/Reagents  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Phosphatase Activity Assays/Reagents   Browse Recombinant/Natural Proteins  
     OriGene Antibodies for GRK5   OriGene shRNA RFP for GRK5  
     OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for GRK5   OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for GRK5  
     OriGene Protein Over-expression Lysate for GRK5   Browse OriGene Fluorogenic Cell Assay Kits  
     OriGene siRNA for GRK5   OriGene 3'-UTR Clone for GRK5  
     OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GRK5   OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for GRK5  
     Browse OriGene GFP tagged cDNA clones in CMV expression vector   Browse OriGene MicroRNA Expression Plasmids  
     Browse OriGene basic RS shRNAs   Browse OriGene validated miRNA SYBR primer pairs  
     OriGene Purified Protein for GRK5   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for GRK5   OriGene Custom Protein Services for GRK5  
     OriGene Custom Immunoassay Development  

         		 
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat GRK5
     QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing GRK5
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GRK5
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat GRK5
     QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GRK5
     QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat GRK5
     GenScript Custom Purified and Recombinant Proteins Services for GRK5 GenScript cDNA clones with any tag delivered in your preferred vector for GRK5
     GenScript Custom Assay Services for GRK5 GenScript Custom Superior Antibodies Services for GRK5
     GenScript Custom overexpressing Cell Line Services for GRK5 CloneReady with Over 120,000 Genes
     Gene Synthesis: Any Gene in Any Vector Vector-based siRNA and miRNA, Ready for Transfection
     Gene Mutant Library, Variants up to 10^11 Plasmid Preparation
     Custom Peptide Services
     Search for Antibodies & Assays

     Regulatory tfbs in GRK5 promoter
     Search Chromatin IP Primers for GRK5
     RT2 qPCR Primer Assay in human, mouse, rat GRK5
     GNC Network for GRK5
     SABiosciences PCR Arrays including human, mouse, rat GRK5
     Search Tocris compounds for GRK5
     Browse Sino Biological Proteins and Antibodies
     cDNA Clones for GRK5
     Antibodies/Proteins Production Services
     Rabbit Monoclonal Antibody Platform
     Bulk Purchasing
     Proteins for GRK5
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
     GRK5 antibodies
     GRK5 proteins
     Antibodies for GRK5
     See all of Abcam's Antibodies, Kits and Proteins for GRK5
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins




     GRK5 Proteins, Antibodies, CLIAs, and ELISAs
     Search LifeMap BioReagents cell lines for GRK5
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GRK5
     SwitchGear 3'UTR luciferase reporter plasmids for GRK5
     SwitchGear Promoter luciferase reporter plasmids for GRK5
     ThermoFisher Antibody for GRK5
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GRK5
           
    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

    View Random Gene

    Category
    VWF
    (GIFTS: 73)
    von Willebrand factor
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 

    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Copyright © 1996-2013 , Weizmann Institute of Science. All Rights Reserved.
    Hot genes      Disease genes      GRK5 gene at Home site.
    hostname: 356977-web1.xennexinc.com index build: 100 solr: 1.4