Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



GRK5 Gene

protein-coding   GIFtS: 69
GCID: GC10P120957

G Protein-Coupled Receptor Kinase 5


(Previous symbol: GPRK5)
  Try our

disease database 

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
G Protein-Coupled Receptor Kinase 51 2
GPRK51 2 3 5
G Protein-Coupled Receptor Kinase GRK52 3
EC 2.7.11.163 8
FP20252
EC 2.7.118

External Ids:    HGNC: 45441   Entrez Gene: 28692   Ensembl: ENSG000001988737   OMIM: 6008705   UniProtKB: P349473   

Export aliases for GRK5 gene to outside databases

Previous GC identifers: GC10P120631 GC10P114596


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for GRK5 Gene:
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily
of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled
receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of
polymorphonuclear leukocytes (PMNs). (provided by RefSeq, Jul 2008)

GeneCards Summary for GRK5 Gene:
GRK5 (G protein-coupled receptor kinase 5) is a protein-coding gene. GO annotations related to this gene include protein kinase C binding and protein serine/threonine kinase activity. An important paralog of this gene is AKT1.

UniProtKB/Swiss-Prot: GRK5_HUMAN, P34947
Function: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of
G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor
desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety
of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4
subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates:
Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits
G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor
of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription.
Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of
ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non
G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro)

Gene Wiki entry for GRK5 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000010.10  NT_030059.14  NC_018921.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the GRK5 gene promoter:
         AML1a   AP-2gamma   Gfi-1   Meis-1b   YY1   CREB   deltaCREB   IRF-7A   Meis-1a   Meis-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidGRK5 promoter sequence
   Search Chromatin IP Primers for GRK5

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat GRK5


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q26.11   Ensembl cytogenetic band:  10q26.11   HGNC cytogenetic band: 10q26.11

GRK5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GRK5 gene location

GeneLoc information about chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P120957:  view genomic region     (about GC identifiers)

Start:
120,967,101 bp from pter      End:
121,215,131 bp from pter
Size:
248,031 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: GRK5_HUMAN, P34947 (See protein sequence)
Recommended Name: G protein-coupled receptor kinase 5  
Size: 590 amino acids; 67787 Da
Subunit: Interacts with ST13 (via the C-terminus 303-319 AA). Interacts with TP53/p53. Interacts with HTR4 (via
C-terminus 330-346 AA); this interaction is promoted by 5-HT (serotonin). Interacts with HDAC5 (By similarity)
Secondary accessions: D3DRD0 Q5T059

Explore the universe of human proteins at neXtProt for GRK5: NX_P34947

Explore proteomics data for GRK5 at MOPED

Post-translational modifications: 

  • Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity1
  • Ubiquitination2 at Lys194
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for GRK5 (P34947) (see all 26)
     MNGGDLK  LNEKQIL  PPFVPDP  LQWKWLE 


    See GRK5 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_005299.1  
    ENSEMBL proteins: 
     ENSP00000376609   ENSP00000358104  
    Reactome Protein details: P34947

    GRK5 Human Recombinant Protein Products:

    EMD Millipore Purified and/or Recombinant GRK5 Protein
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for GRK5
    OriGene Purified Protein for GRK5
    OriGene Protein Over-expression Lysate for GRK5
    OriGene MassSpec for GRK5
    OriGene Custom Protein Services for GRK5
    GenScript Custom Purified and Recombinant Proteins Services for GRK5
    Novus Biologicals GRK5 Proteins
    Sino Biological Recombinant Protein for GRK5
    Sino Biological Cell Lysate for GRK5
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for GRK5

     
    Search eBioscience for Proteins for GRK5 

    GRK5 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    R&D Systems Antibodies for GRK5
    OriGene Antibodies for GRK5
    OriGene Custom Antibody Services for GRK5
    Novus Biologicals GRK5 Antibodies
    Abcam antibodies for GRK5
    Cloud-Clone Corp. Antibodies for GRK5
    ThermoFisher Antibody for GRK5
    LSBio Antibodies in human, mouse, rat for GRK5

    GRK5 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for GRK5
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for GRK5
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for GRK5
    Cloud-Clone Corp. CLIAs for GRK5
    Search eBioscience for ELISAs for GRK5 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    IUPHAR Guide to PHARMACOLOGY protein family classification: G protein-coupled receptor kinase 5
    G protein coupled receptor kinases
    GRK subfamily

    Selected InterPro protein domains (see all 8):
     IPR017441 Protein_kinase_ATP_BS
     IPR000239 GPCR_kinase
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR011009 Kinase-like_dom
     IPR000719 Prot_kinase_dom

    Graphical View of Domain Structure for InterPro Entry P34947

    ProtoNet protein and cluster: P34947

    3 Blocks protein domains:
    IPB000239 GPCR kinase signature
    IPB000342 Regulator of G-protein signalling (RGS) protein signature
    IPB000961 Protein kinase C-terminal domain


    UniProtKB/Swiss-Prot: GRK5_HUMAN, P34947
    Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily
    Similarity: Contains 1 AGC-kinase C-terminal domain
    Similarity: Contains 1 protein kinase domain
    Similarity: Contains 1 RGS domain


    Find genes that share domains with GRK5           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: GRK5_HUMAN, P34947
    Function: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of
    G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor
    desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety
    of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4
    subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates:
    Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits
    G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor
    of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription.
    Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of
    ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non
    G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro)
    Catalytic activity: ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate
    Enzyme regulation: Inhibited by calmodulin with an IC(50) of 50 nM. Calmodulin inhibits GRK5 association with
    receptor and phospholipid
    Induction: Overexpressed during heart failure

         Enzyme Numbers (IUBMB): EC 2.7.11.161 2 EC 2.7.112

         Gene Ontology (GO): Selected molecular function terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity ----
    GO:0004674protein serine/threonine kinase activity IDA--
    GO:0004703G-protein coupled receptor kinase activity IEA--
    GO:0004713protein tyrosine kinase activity ----
    GO:0005080protein kinase C binding TAS9813065
         
    Find genes that share ontologies with GRK5           About GenesLikeMe


    Phenotypes:
         3 GenomeRNAi human phenotypes for GRK5:
     Decreased Wnt reporter activit  Increased cell death HMECs cel  Increased mitotic index 

         9 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Grk5):
     behavior/neurological  digestive/alimentary  endocrine/exocrine gland  homeostasis/metabolism  integument 
     muscle  nervous system  normal  respiratory system 

    Find genes that share phenotypes with GRK5           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Grk5tm1Rjl for GRK5

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for GRK5
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for GRK5

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for GRK5
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for GRK5

    miRNA
    Products:
        
    miRTarBase miRNAs that target GRK5:
    hsa-mir-941 (MIRT036566)

    Block miRNA regulation of human, mouse, rat GRK5 using miScript Target Protectors
    8 qRT-PCR Assays for microRNAs that regulate GRK5:
    hsa-miR-539 hsa-miR-106a* hsa-miR-1304 hsa-miR-3168 hsa-miR-135b hsa-miR-1299 hsa-miR-4277 hsa-miR-135a
    SwitchGear 3'UTR luciferase reporter plasmidGRK5 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for GRK5
    Predesigned siRNA for gene silencing in human, mouse, rat GRK5

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for GRK5

    Clone
    Products:
         
    OriGene clones in human, mouse for GRK5 (see all 6)
    OriGene ORF clones in mouse, rat for GRK5
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: GRK5 (NM_005308)
    Sino Biological Human cDNA Clone for GRK5
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for GRK5
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GRK5
    Addgene plasmids for GRK5 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for GRK5
    Browse ESI BIO Cell Lines and PureStem Progenitors for GRK5 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GRK5


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    GRK5_HUMAN, P34947: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Note=Predominantly localized
    at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus
    localization is regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    plasma membrane5
    cytosol3
    cytoskeleton1
    endosome1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IEA--
    GO:0005737cytoplasm IEA--
    GO:0005886plasma membrane IDA14976207

    Find genes that share ontologies with GRK5           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for GRK5 About   (see all 9)  
    See pathways by source

    SuperPathContained pathways About
    1Chemokine Signaling
    Chemokine signaling pathway0.33
    Chemokine Signaling0.33
    2Signaling by GPCR
    Signaling by GPCR0.90
    Signal Transduction0.58
    GPCR downstream signaling0.90
    3Gastrin-CREB signalling pathway via PKC and MAPK
    Gastrin-CREB signalling pathway via PKC and MAPK0.90
    G alpha (q) signalling events0.90
    4Myometrial Relaxation and Contraction Pathways
    Myometrial Relaxation and Contraction Pathways0.43
    Calcium Regulation in the Cardiac Cell0.43
    5Circadian entrainment
    Morphine addiction0.53


    Find genes that share SuperPaths with GRK5           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    2 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for GRK5
        Chemokine Signaling
    Colorectal Cancer Metastasis

    1 GeneGo (Thomson Reuters) Pathway for GRK5
        Development Angiotensin signaling via beta-Arrestin

    3 BioSystems Pathways for GRK5
        Myometrial Relaxation and Contraction Pathways
    Calcium Regulation in the Cardiac Cell
    Alpha-synuclein signaling


    1 Reactome Pathway for GRK5
        G alpha (q) signalling events


    3 Kegg Pathways  (Kegg details for GRK5):
        Chemokine signaling pathway
    Endocytosis
    Morphine addiction

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for GRK5
    Interactions:

        GeneGlobe Interaction Network for GRK5

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for GRK5 (P349471, 2, 3 ENSP000003766094) via UniProtKB, MINT, STRING, and/or I2D (see all 162)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    Htr4P972881, 2EBI-7149314,EBI-7149283 MINT-7943386 MINT-7943438 MINT-7943519 MINT-7943403 MINT-7943553 MINT-7943457 MINT-7943535 MINT-7943476 MINT-7943501
    ARRB1P494072, ENSP000003771414MINT-7943519 MINT-7943553 MINT-7943535 MINT-7943476 MINT-7943501 STRING: ENSP00000377141
    CALM1P621583, ENSP000003494674I2D: score=1 STRING: ENSP00000349467
    CALM2P621583I2D: score=1 
    CALM3P621583I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006468protein phosphorylation ----
    GO:0006915apoptotic process IEA--
    GO:0007165signal transduction ----
    GO:0007188adenylate cyclase-modulating G-protein coupled receptor signaling pathway TAS8626574
    GO:0007217tachykinin receptor signaling pathway IDA17986524

    Find genes that share ontologies with GRK5           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for GRK5

    Selected Novoseek inferred chemical compound relationships for GRK5 gene (see all 15)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    pip2 48.6 6 8798768 (4), 10818226 (1)
    threonine 46.5 1 19661922 (1)
    serine 37.6 1 19661922 (1)
    dextran sulfate 31.4 3 8288567 (1), 8077221 (1)
    isoproterenol 28.5 1 18034461 (1)
    leukotriene b4 17 1 12077128 (1)
    phospholipid 10.2 7 8144599 (3), 9218466 (1), 9813065 (1)
    phosphoinositide 6.89 1 10861009 (1)
    atp 0 1 8288567 (1)
    sucrose 0 1 8170959 (1)

    3 PharmGKB related drug/compound annotations for GRK5 gene    About this table
    Drug/compound PharmGKB Annotation
    Beta Blocking AgentsCA  
    citalopramCA  
    escitalopramCA  



    Find genes that share compounds with GRK5           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for GRK5 gene: 
    NM_005308.2  

    Unigene Cluster for GRK5:

    G protein-coupled receptor kinase 5
    Hs.524625  [show with all ESTs]
    Unigene Representative Sequence: NM_005308
    3 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000392870(uc001led.3 uc009xzh.3 uc010qta.1) ENST00000473264
    ENST00000369108
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat GRK5 using miScript Target Protectors
    8 qRT-PCR Assays for microRNAs that regulate GRK5:
    hsa-miR-539 hsa-miR-106a* hsa-miR-1304 hsa-miR-3168 hsa-miR-135b hsa-miR-1299 hsa-miR-4277 hsa-miR-135a
    SwitchGear 3'UTR luciferase reporter plasmidGRK5 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for GRK5
    Predesigned siRNA for gene silencing in human, mouse, rat GRK5
    Clone
    Products:
         
    OriGene clones in human, mouse for GRK5 (see all 6)
    OriGene ORF clones in mouse, rat for GRK5
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: GRK5 (NM_005308)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for GRK5
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GRK5
    Addgene plasmids for GRK5 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for GRK5
    OriGene qSTAR qPCR primer pairs in human, mouse for GRK5
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat GRK5
      QuantiTect SYBR Green Assays in human, mouse, rat GRK5
      QuantiFast Probe-based Assays in human, mouse, rat GRK5

    Additional mRNA sequence: 

    AF370369.1 AK298261.1 AK301450.1 AK309761.1 AK313012.1 BC018116.1 BC064506.1 BC114448.1 
    BC127602.1 L15388.1 

    8 DOTS entries:

    DT.40110887  DT.102819582  DT.95370282  DT.91640334  DT.121250959  DT.102833238  DT.95204296  DT.97844869 

    Selected AceView cDNA sequences (see all 153):

    AI358974 AI200481 AW206339 BM927442 BQ061150 W77864 BU527897 BQ071007 
    AA059452 BU146436 AI348152 BQ061253 AA059451 BF195014 BQ186585 BM719846 
    BP345276 BQ066445 BU526699 BQ882260 BQ065277 NM_005308 BU681200 BQ071108 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    GRK5 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CACGTCCTCC
    GRK5 Expression
    About this image


    GRK5 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 5) fully expand
     
     Lung (Respiratory System)
             Alveolar type II cells
     
     Testis (Reproductive System)
             Testicular Interstitial Cells Testis Interstitium
     
     NULL (Uncategorized)
             Endoderm progenitor-like cells
     
     Lymph (Hematopoietic System)
     
     Spleen (Hematopoietic System)
    GRK5 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    GRK5 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.524625

    UniProtKB/Swiss-Prot: GRK5_HUMAN, P34947
    Tissue specificity: Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney

        Custom PCR Arrays for GRK5
    Primer
    Products:
    OriGene qPCR primer pairs and template standards for GRK5
    OriGene qSTAR qPCR primer pairs in human, mouse for GRK5
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat GRK5
    QuantiTect SYBR Green Assays in human, mouse, rat GRK5
    QuantiFast Probe-based Assays in human, mouse, rat GRK5
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GRK5

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for GRK5 gene from Selected species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Grk51 , 5 G protein-coupled receptor kinase 51, 5 88.31(n)1
    95.25(a)1
      19 (56.52 cM)5
    147731  NM_018869.31  NP_061357.31 
     608897495 
    chicken
    (Gallus gallus)
    Aves GRK51 G protein-coupled receptor kinase 5 80.45(n)
    91.02(a)
      423929  XM_421789.3  XP_421789.2 
    lizard
    (Anolis carolinensis)
    Reptilia GRK56
    G protein-coupled receptor kinase 5
    91(a)
    1 ↔ 1
    GL343399.1(562688-882165)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia LOC1004973441 G protein-coupled receptor kinase 5-like 77.85(n)
    85.76(a)
      100497344  XM_002940348.2  XP_002940394.1 
    zebrafish
    (Danio rerio)
    Actinopterygii BQ419577.12   -- 75.25(n)    BQ419577.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Gprk21 G protein-coupled receptor kinase 2 63.9(n)
    65.05(a)
      49045  NM_057519.4  NP_476867.1 
    worm
    (Caenorhabditis elegans)
    Secernentea grk-11 grk-1 57.49(n)
    59.6(a)
      181212  NM_077275.4  NP_509676.1 
    rice
    (Oryza sativa)
    Liliopsida Os.475052 Oryza sativa (japonica cultivar-group) cDNA cloneJ more 75.01(n)    AK065005.1 


    ENSEMBL Gene Tree for GRK5 (if available)
    TreeFam Gene Tree for GRK5 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for GRK5 gene
    AKT12  GRK72  RPS6KB12  GRK12  SGK32  GRK42  GRK62  C8orf44-SGK32  
    SGK22  ADRBK22  AKT32  SGK12  RPS6KB22  ADRBK12  AKT22  
    Selected SIMAP similar genes for GRK5 using alignment to 4 protein entries:     GRK5_HUMAN (see all proteins) (see all similar genes):
    GRK6    GRK4    GRK1    GRK7    MAP3K11    PRKD3
    MAPK8    MARK4    PRKCH    PRKCZ    RPS6KA5    ADRBK1
    CDK7    PDPK2    PRKY    PkB-like 1    SGK3    ADRBK2

    Find genes that share paralogs with GRK5           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Polymorphic Variants from UniProtKB/Swiss-Prot
    GRK5_HUMAN, P34947: Variant Leu-41 variant is rare in European-Americans individuals but common in African-Americans
    individuals (40% of the African-American individuals studied carry at least one allele). Variant leu-41 is
    associated with decreased mortality in African-Americans with heart failure or cardiac ischemia


    Selected SNPs for GRK5 (see all 5120)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 10 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0405224
    A lung neuroendocrine carcinoma sample4--see VAR_0405222 D E mis40--------
    rs353332461,2
    C--114682646(+) AAAAA-/AAA/AA
    AA
    /AAAAA
    GTCAA
    2 -- int1 cds11NA 2
    rs1412672351,2
    --120965255(+) CCCGGC/GCCAAG 1 -- us2k10--------
    rs1822881861,2
    --120965296(+) GGCATA/TCAAAA 1 -- us2k10--------
    rs1392221281,2
    C--120965304(+) AAAAT-/TTTC  
            
    TTTCT
    1 -- us2k10--------
    rs2009668501,2
    --120965309(+) TTTCT-/TTCTTTTT 1 -- us2k10--------
    rs2016061851,2
    --120965311(+) TCTTT-/CTTTTT 1 -- us2k10--------
    rs111988211,2
    C,F--120965341(+) AAACAA/GACTTC 1 -- us2k15Minor allele frequency- G:0.11WA CSA NA EA 362
    rs795243471,2
    C,F--120965385(+) ACCAGC/TGTGGT 1 -- us2k11Minor allele frequency- T:0.07EA 120
    rs1869072311,2
    --120965615(+) GATAAA/GTGAGG 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for GRK5 (120967101 - 121215131 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for GRK5 (see all 30):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2671031CNV Deletion23128226
    esv4668CNV Deletion18987735
    dgv122e201CNV Deletion23290073
    esv2741851CNV Deletion23290073
    esv1002455CNV Deletion20482838
    esv2741840CNV Deletion23290073
    esv2741672CNV Deletion23290073
    esv2741650CNV Deletion23290073
    esv2670333CNV Deletion23128226
    dgv123e201CNV Deletion23290073

    Human Gene Mutation Database (HGMD): GRK5
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing GRK5
    DNA2.0 Custom Variant and Variant Library Synthesis for GRK5

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 600870    OMIM disorders: --


    Find genes that share disorders with GRK5           About GenesLikeMe

    4 Novoseek inferred disease relationships for GRK5 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    heart failure 33.8 2 20443857 (1), 18425130 (1)
    heart failure congestive 12.1 1 10448093 (1)
    huntington disease 9.95 1 8415712 (1)
    neurodegenerative diseases 0 1 17146290 (1)

    Genetic Association Database (GAD): GRK5
    Human Genome Epidemiology (HuGE) Navigator: GRK5 (17 documents)

    Export disorders for GRK5 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for GRK5 gene, integrated from 10 sources (see all 168):
    (articles sorted by number of sources associating them with GRK5)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Cloning and expression of GRK5: a member of the G protein-coupled receptor kinase family. (PubMed id 7685906)1, 2, 3, 9 Kunapuli P. and Benovic J.L. (Proc. Natl. Acad. Sci. U.S.A. 1993)
    2. A GRK5 polymorphism that inhibits beta-adrenergic receptor signaling is protective in heart failure. (PubMed id 18425130)1, 2, 4, 9 Liggett S.B.... Dorn G.W. II (Nat. Med. 2008)
    3. G protein-coupled receptor kinase 5 contains a DNA-binding nuclear localization sequence. (PubMed id 15542828)1, 2, 9 Johnson L.R.... Pitcher J.A. (Mol. Cell. Biol. 2004)
    4. A predicted amphipathic helix mediates plasma membrane localization of GRK5. (PubMed id 14976207)1, 2, 9 Thiyagarajan M.M.... Wedegaertner P.B. (J. Biol. Chem. 2004)
    5. Phospholipid-stimulated autophosphorylation activates the G protein- coupled receptor kinase GRK5. (PubMed id 8144599)1, 2, 9 Kunapuli P.... Benovic J.L. (J. Biol. Chem. 1994)
    6. Role for the regulator of G-protein signaling homology domain of G protein-coupled receptor kinases 5 and 6 in beta 2-adrenergic receptor and rhodopsin phosphorylation. (PubMed id 20038610)1, 2, 9 Baameur F.... Clark R.B. (Mol. Pharmacol. 2010)
    7. Beta-arrestin1 phosphorylation by GRK5 regulates G protein- independent 5-HT4 receptor signalling. (PubMed id 19661922)1, 2, 9 Barthet G....Dumuis A. (EMBO J. 2009)
    8. L41Q polymorphism of the G protein coupled receptor kinase 5 is associated with left ventricular apical ballooning syndrome. (PubMed id 20023040)1, 4, 9 Spinelli L....Trimarco B. (Eur. J. Heart Fail. 2010)
    9. Clinical and genetic modifiers of long-term survival in heart failure. (PubMed id 19628119)1, 4, 9 Cresci S....Dorn G.W. (J. Am. Coll. Cardiol. 2009)
    10. G protein-coupled receptor kinase 5 phosphorylation of Hip regulates internalization of the chemokine receptor CXCR4. (PubMed id 21728385)1, 2 Barker B.L. and Benovic J.L. (Biochemistry 2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 2869 HGNC: 4544 AceView: GRK5 Ensembl:ENSG00000198873 euGenes: HUgn2869
    ECgene: GRK5 Kegg: 2869 H-InvDB: GRK5

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for GRK5 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for GRK5 gene:
    Search GeneIP for patents involving GRK5

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
     EMD Millipore Purified and/or Recombinant GRK5 Protein
     Browse Small Molecules at EMD Millipore
     Browse Kits and Assays available from EMD Millipore
     
     EMD Millipore genomic analysis products

      
     Antibodies for GRK5 (GRK5)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for GRK5   OriGene RNAi products in human, mouse, rat for GRK5  
     OriGene qPCR primer pairs and template standards for GRK5   OriGene Protein Over-expression Lysate for GRK5  
     OriGene MassSpec something-or-other for GRK5   OriGene clones in human, mouse for GRK5  
     OriGene qSTAR qPCR primer pairs in human, mouse for GRK5   OriGene Purified Protein for GRK5  
     OriGene ORF clones in mouse, rat for GRK5   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for GRK5   OriGene Custom Protein Services for GRK5  

     
     
     Block miRNA regulation of human, mouse, rat GRK5 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing GRK5
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat GRK5 Predesigned siRNA for gene silencing in human, mouse, rat GRK5
     QuantiFast Probe-based Assays in human, mouse, rat GRK5 QuantiTect SYBR Green Assays in human, mouse, rat GRK5
     Custom PCR Arrays for GRK5 Search Chromatin IP Primers for GRK5
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat GRK5  GeneGlobe Interaction Network for GRK5
     Regulatory tfbs in GRK5 promoter
     GenScript Custom Purified and Recombinant Proteins Services for GRK5 GenScript cDNA clones with any tag delivered in your preferred vector for GRK5
     GenScript Custom Assay Services for GRK5 GenScript Custom overexpressing Cell Line Services for GRK5
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Search Tocris compounds for GRK5
     Proteins for GRK5
     Cell Lysates for GRK5
     cDNA Clones for GRK5
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Proteins for GRK5
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     GRK5 antibodies
     GRK5 proteins
     Antibodies for GRK5
     See all of Abcam's Antibodies, Kits and Proteins for GRK5
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for GRK5
     Antibodies for GRK5
     ELISAs for GRK5
     CLIAs for GRK5



     Browse ESI BIO Cell Lines and PureStem Progenitors for GRK5
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GRK5
     SwitchGear 3'UTR luciferase reporter plasmids for GRK5
     SwitchGear Promoter luciferase reporter plasmids for GRK5
     ThermoFisher Antibody for GRK5
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GRK5
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for GRK5
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for GRK5
     LSBio Antibodies in human, mouse, rat for GRK5
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
     Addgene plasmids for GRK5
      Search eBioscience for proteins for GRK5
      Search eBioscience for elisas for GRK5
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      GRK5 gene at Home site.
    Version: 3.12.224 13 Oct 2014
    hostname: 356980-web2.xennexinc.com index build: 128 solr: 1.4