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GREB1 Gene

protein-coding   GIFtS: 43

GC02P011591
GREB1 protein
symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
KIAA0575 2, 3
OTTHUMP00000180581 2
OTTHUMP00000180583 2
Descriptions
GREB1 protein 2
Gene regulated in breast cancer 1 protein 3
gene regulated by estrogen in breast cancer protein 2
External Ids
Entrez Gene: 96872
UniProtKB: Q4ZG553
Ensembl: ENSG000001962087
Search outside databases for aliases for GREB1 gene

Previous GC identifers: GC02P011537 GC02P011725 GC02P011695 GC02P011624

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for GREB1:
This gene is an estrogen-responsive gene that is an early response gene in the estrogen
receptor-regulated pathway. It is thought to play an important role in hormone-responsive tissues
and cancer. Three alternatively spliced transcript variants encoding distinct isoforms have been
found for this gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: GREB1_HUMAN, Q4ZG55
Function: Unknown. Primary target for estrogen receptor regulation. Acts as a regulator of
hormone-dependent cancer growth in breast and prostate cancers

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the GREB1 gene  

Entrez Gene cytogenetic band: 2p25.1   Ensembl cytogenetic band:  2p25.1   

GREB1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P011591:     (about GC identifiers)

Start:
11,591,693 bp from pter
End:
11,700,365 bp from pter
Size:
108,673 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_005334.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: GREB1_HUMAN, Q4ZG55 (See protein sequence)
Recommended Name: Protein GREB1  
Size: 1949 amino acids; 216467 Da
Subcellular location: Membrane; Single-pass membrane protein (Potential)
Secondary accessions: A6NHD0 A6NKN0 O60321 Q7Z5S2 Q9H2Q6 Q9H2Q7 Q9H2Q8
Alternative splicing: 4 isoforms:  Q4ZG55-1   Q4ZG55-2   Q4ZG55-3   Q4ZG55-4   

REFSEQ proteins (3 alternative transcripts): 
NP_055483.2  NP_149081.1  NP_683701.2  


ENSEMBL proteins: 
ENSP00000374473 ENSP00000374475 ENSP00000379429 ENSP00000370892 ENSP00000263834 ENSP00000379432 
ENSP00000370896 ENSP00000374474 ENSP00000370894 ENSP00000234142 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (GREB1)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
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Antibodies for GREB1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (GREB1), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for GREB1

Assays for GREB1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ProtoNet protein and cluster: Q4ZG55

UniProtKB/Swiss-Prot: GREB1_HUMAN, Q4ZG55
Similarity: Belongs to the GREB1 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (GREB1)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_148903

              Applied Biosystems Silencer® siRNAs for GREB1

              Sigma-Aldrich siRNA for GREB1  
                     Sigma-Aldrich shRNA for GREB1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_014668
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_014668
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_014668 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_014668

UniProtKB/Swiss-Prot: GREB1_HUMAN, Q4ZG55
Function: Unknown. Primary target for estrogen receptor regulation. Acts as a regulator of
hormone-dependent cancer growth in breast and prostate cancers
Induction: Estrogen-responsive; regulated by estrogen receptors. Regulated by androgens. The
regulatory region of the gene contains 3 estrogen-responsive elements

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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 Gene Network CentralTM Interacting Genes and Proteins Network for  GREB1 


1 Interacting protein for GREB1 (ENSP000002341423) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
ESR1ENSP000002062493STRING (score=.968)
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for GREB1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (GREB1)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_148903

              Sigma-Aldrich siRNA for GREB1  
                     Sigma-Aldrich shRNA for GREB1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_014668  NM_033090  NM_148903  

REFSEQ mRNAs for GREB1 gene (3 alternative transcripts): 

NM_014668.3   NM_033090.2   NM_148903.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_014668  NM_033090  NM_148903  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_014668
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_014668
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_014668 

Additional cDNA sequence: 

AB011147.1 AF245388.1 AF245389.1 AF245390.1 AK056886.1 AK126460.1 BC034455.1 BC054502.1 
BC071853.1 

13 DOTS entries:

DT.111665  DT.91662353  DT.40206004  DT.100008627  DT.92034971  DT.95292353  DT.100737547  DT.101962569 
DT.86846430  DT.86857727  DT.95313309  DT.121031998  DT.91921337 

24/65 AceView cDNA sequences (see all 65 ):

BC034455 AK056886 NM_014668 AA348259 NM_033090 AI678417 NM_148903 BC071853 
BG151550 R50088 CD625896 AB011147 AF245389 AF245388 BF513255 BU934253 
BX641647 BU784607 BX282687 BU955811 BX641662 BF196386 AF245390 BM271782 

highest scoring ESTs for GREB1:

AB011147 AA348259 AA348260 AI678417 AI870551 AW576820 BC054502 BU784607 BU786335 BU941900 

Unigene Cluster for GREB1:

GREB1 protein
Hs.467733  [show with all ESTs]
Unigene Representative Sequence: NM_014668


GeneLoc Exon Structure

10 Ensembl transcripts including schematic representations:
ENST00000389823  ENST00000389825  ENST00000396123  ENST00000381483  ENST00000263834  ENST00000396126  
ENST00000381486  ENST00000389824  ENST00000381484  ENST00000234142  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

GREB1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for GREB1

1 / 2 / 3

7 probe-sets matching GREB1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
38875_r_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AB011147 1.00 1.00 1.00 1

210855_at2, 3 U133-A 1 1.00 1.00 -- -- AF245390 0.60 0.75 0.68 1

205862_at2, 3 U133-A 1 1.00 1.00 -- -- NM_014668 0.60 1.00 0.82 1

210562_at2, 3 U133-A 1 1.00 1.00 -- -- AF245389 0.60 0.75 0.68 1

210855_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

205862_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

210562_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  GREB1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.467733

Expression variation in blood from EXPOLDB for GREB1

UniProtKB/Swiss-Prot: GREB1_HUMAN, Q4ZG55
Tissue specificity: Expressed in proliferating prostatic tissue and prostate cancer

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for GREB1 gene from 5/8 species (see all 8 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
LOC6100071   -- similar to GREB1 protein isoform a 88.59(n)
92.19(a)
610007  XM_847384.1  XP_852477.1 
chimpanzee
(Pan troglodytes)
LOC4590341   -- GREB1 protein 98.08(n)
98.1(a)
459034  XM_515304.2  XP_515304.2 
cow
(Bos taurus)
LOC5082041   -- similar to GREB1 protein 87.59(n)
91.49(a)
508204  XM_584947.3  XP_584947.3 
mouse
(Mus musculus)
Greb11, 5 125
gene regulated by estrogen in breast cancer protein1, 5 85.25(n)1
88.93(a)1
2685271  NM_015764.21  NP_056579.11 
 AB0932485  AF1804705  (see all 22)
chicken
(Gallus gallus)
LOC4219441   -- similar to GREB1 protein 72.08(n)
79.79(a)
421944  XM_419956.2  XP_419956.2 
About this table        Species with no ortholog for GREB1

ENSEMBL Gene Tree for GREB1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for GREB1 gene
KIAA17722  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/745 NCBI SNPs in GREB1 are shown (see all 745 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 232)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 2 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs23580401,2
A,C,F,H,O11649225(+) AGCAAG/TCTGAT 2 -- int1 ut3111Minor allele frequency- T:0.39EU EA WA NA 978
rs28843741,2
A,C,F,H,O11649297(+) CTATAC/TGTCAA 2 -- ut31 int1 ese318Minor allele frequency- T:0.39EU EA WA NA 1656
rs46697521,2
A,C,F,H11645439(+) TGTAGC/GTAAAG 3 -- int1 ut317Minor allele frequency- G:0.38EU EA WA NA 558
rs46697511,2
A,C,F,H11624144(+) GGGGGT/CCAAGT 3 A/V mis18Minor allele frequency- C:0.41EU EA WA 838
rs10363931,2
A,C,F,H11700757(-) TGGAGT/CATGGG 1 -- ng317Minor allele frequency- C:0.23EU EA WA NA 522
rs67283651,2
A,C,F,H11591268(+) CAAGCC/AGGTGA 1 -- ng514Minor allele frequency- A:0.07EU EA WA 416
--
rs67448171,2
A,C,F,H11643359(+) CCTGCG/ATGCCG 3 M/V mis1 ese34Minor allele frequency- A:0.10EU EA WA 412
rs116850321,2
A,C,F,H11591746(+) AATGGA/CAGAAG 1 -- ut51 trp310Minor allele frequency- C:0.41EU EA WA 1006
rs109297571,2
A,C,F,H11620112(+) CCCAAA/CCCCTT 3 N/T mis1 ese38Minor allele frequency- C:0.45EU EA WA 834
rs18655741,2
A,C,F,H11598889(-) GTCTAC/TAAGAG 3 -- int1 ng514Minor allele frequency- T:0.30EU EA WA 418
About this table

HapMap Linkage Disequilibrium images for GREB1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 611736

Human Genome Epidemiology Navigator: GREB1 (2 documents)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/15 PubMed articles for GREB1 gene (see all 15 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 9687 AceView: GREB1 Ensembl:ENSG00000196208 euGenes: HUgn9687
(According to HUGE)
About This Section

HUGE: KIAA0575
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for GREB1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for GREB1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for GREB1 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for GREB1 Browse small molecules at Sigma-Aldrich
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 Search Novus for antibodies for GREB1

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