Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



GPX4 Gene

protein-coding   GIFtS: 72
GCID: GC19P001103

Glutathione Peroxidase 4

(Previous name: glutathione peroxidase 4 (phospholipid hydroperoxidase))
Microbiology & Infectious Diseases Congress
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Glutathione Peroxidase 41 2 3     MCSP2
Phospholipid Hydroperoxidase1 2     Phospholipid Hydroperoxide Glutathione Peroxidase, Mitochondrial2
GPx-42 3     snGPx2
GSHPx-42 3     snPHGPx2
PHGPx2 3     Sperm Nucleus Glutathione Peroxidase2
EC 1.11.1.123 8     EC 1.11.18
Glutathione Peroxidase 4 (Phospholipid Hydroperoxidase)1     

External Ids:    HGNC: 45561   Entrez Gene: 28792   Ensembl: ENSG000001674687   OMIM: 1383225   UniProtKB: P369693   

Export aliases for GPX4 gene to outside databases

Previous GC identifers: GC19P001181 GC19P001043 GC19P001054 GC19P000878


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for GPX4 Gene:
This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the
reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions
in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a
selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been
identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a
structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces
proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription
initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally
verified. Alternative splicing results in multiple transcript variants. (provided by RefSeq, Jan 2014)

GeneCards Summary for GPX4 Gene:
GPX4 (glutathione peroxidase 4) is a protein-coding gene. Diseases associated with GPX4 include spermatic cord torsion, and keshan disease. GO annotations related to this gene include phospholipid-hydroperoxide glutathione peroxidase activity and glutathione peroxidase activity. An important paralog of this gene is GPX6.

UniProtKB/Swiss-Prot: GPX4_HUMAN, P36969
Function: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development
and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid
hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity)

Gene Wiki entry for GPX4 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000019.10  NC_018930.2  NT_011295.12  
Regulatory elements:
   Regulatory transcription factor binding sites in the GPX4 gene promoter:
         p53   Sp1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidGPX4 promoter sequence
   Search Chromatin IP Primers for GPX4

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat GPX4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19p13.3   Ensembl cytogenetic band:  19p13.3   HGNC cytogenetic band: 19p13.3

GPX4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GPX4 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P001103:  view genomic region     (about GC identifiers)

Start:
1,103,936 bp from pter      End:
1,106,787 bp from pter
Size:
2,852 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: GPX4_HUMAN, P36969 (See protein sequence)
Recommended Name: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial precursor  
Size: 197 amino acids; 22175 Da
Subunit: Monomer. Has a tendency to form higher mass oligomers
2 PDB 3D structures from and Proteopedia for GPX4:
2GS3 (3D)        2OBI (3D)    
Secondary accessions: O43381 Q6PJ59 Q9UPK2
Alternative initiation: 2 isoforms:  P36969-1   P36969-2   

Explore the universe of human proteins at neXtProt for GPX4: NX_P36969

Explore proteomics data for GPX4 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys162, Lys167
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for GPX4 (P36969) (see all 10)
     VIEKDLP  FPCNQFG  FDMFSKI  LAFPCNQF 


    See GPX4 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001034936.1  NP_001034937.1  NP_002076.2  

    ENSEMBL proteins: 
     ENSP00000346103   ENSP00000466872   ENSP00000465828   ENSP00000464989   ENSP00000473614  
     ENSP00000468349   ENSP00000467900  
    Reactome Protein details: P36969

    GPX4 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for GPX4
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for GPX4
    OriGene Custom MassSpec
    OriGene Custom Protein Services for GPX4
    GenScript Custom Purified and Recombinant Proteins Services for GPX4
    Novus Biologicals GPX4 Protein
    Novus Biologicals GPX4 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for GPX4

    GPX4 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of GPX4
    R&D Systems Antibodies for GPX4 (Glutathione Peroxidase 4/GPX4)
    OriGene Antibodies for GPX4
    OriGene Custom Antibody Services for GPX4
    Novus Biologicals GPX4 Antibodies
    Abcam antibodies for GPX4
    Cloud-Clone Corp. Antibodies for GPX4
    ThermoFisher Antibody for GPX4
    LSBio Antibodies in human, mouse, rat for GPX4

    GPX4 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for GPX4
    R&D Systems DNA Damage/Repair Kits & Reagents for GPX4 (Glutathione Peroxidase 4/GPX4)
    GenScript Custom Assay Services for GPX4
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for GPX4
    Cloud-Clone Corp. CLIAs for GPX4


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    2 InterPro protein domains:
     IPR012336 Thioredoxin-like_fold
     IPR000889 Glutathione_peroxidase

    Graphical View of Domain Structure for InterPro Entry P36969

    ProtoNet protein and cluster: P36969

    1 Blocks protein domain: IPB000889 Glutathione peroxidase

    UniProtKB/Swiss-Prot: GPX4_HUMAN, P36969
    Similarity: Belongs to the glutathione peroxidase family


    GPX4 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: GPX4_HUMAN, P36969
    Function: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development
    and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid
    hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity)
    Catalytic activity: 2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H(2)O

         Genatlas biochemistry entry for GPX4:
    glutathione peroxidase 4,expressed in testis,catabolic pathway of activated oxygen species,free radical
    detoxification

         Enzyme Numbers (IUBMB): EC 1.11.1.121 2 EC 1.11.12

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004602glutathione peroxidase activity TAS9705830
    GO:0008430selenium binding IEA--
    GO:0043295glutathione binding IEA--
    GO:0047066phospholipid-hydroperoxide glutathione peroxidase activity IEA--
         
    GPX4 for ontologies           About GeneDecksing


    Phenotypes:
         2 GenomeRNAi human phenotypes for GPX4:
     Synthetic lethal with Ras  Wnt reporter downregulated 

         9 MGI mutant phenotypes (inferred from 8 alleles(MGI details for Gpx4):
     behavior/neurological  cellular  embryogenesis  growth/size/body  liver/biliary system 
     mortality/aging  nervous system  normal  reproductive system 

    GPX4 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for GPX4: Gpx4tm1Yana Gpx4tm2.1Marc Gpx4tm1Marc Gpx4tm1Prol

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for GPX4
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for GPX4

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for GPX4
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for GPX4

    miRNA
    Products:
        
    miRTarBase miRNAs that target GPX4:
    hsa-mir-124-3p (MIRT046107), hsa-mir-26b-5p (MIRT029349)

    Block miRNA regulation of human, mouse, rat GPX4 using miScript Target Protectors
    3 qRT-PCR Assays for microRNAs that regulate GPX4:
    hsa-miR-188-3p hsa-miR-346 hsa-miR-3667-3p
    SwitchGear 3'UTR luciferase reporter plasmidGPX4 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for GPX4
    Predesigned siRNA for gene silencing in human, mouse, rat GPX4

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for GPX4

    Clone
    Products:
         
    OriGene clones in human, mouse for GPX4 (see all 20)
    OriGene ORF clones in mouse, rat for GPX4
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): GPX4 (NM_001039848)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for GPX4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GPX4

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for GPX4
    Browse ESI BIO Cell Lines and PureStem Progenitors for GPX4 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPX4


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    GPX4_HUMAN, P36969: Isoform Mitochondrial: Mitochondrion
    GPX4_HUMAN, P36969: Isoform Cytoplasmic: Cytoplasm
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    mitochondrion5
    cytosol4
    extracellular3
    plasma membrane3
    cytoskeleton2
    endoplasmic reticulum2
    nucleus2
    peroxisome2
    vacuole2
    lysosome1

    Gene Ontology (GO): Selected cellular component terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus ----
    GO:0005635nuclear envelope IEA--
    GO:0005739mitochondrion TAS9705830
    GO:0005743mitochondrial inner membrane IEA--
    GO:0005829cytosol TAS--

    GPX4 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for GPX4 About   (see all 7)                                                                                              See pathways by source

    SuperPathContained pathways About
    1Glutathione metabolism
    Glutathione metabolism0.65
    Glutathione metabolism0.45
    Glutathione metabolism0.65
    glutathione redox reactions I0.00
    2Arachidonic acid metabolism
    Arachidonic acid metabolism0.40
    Synthesis of 5-eicosatetraenoic acids0.00
    HETE and HPETE biosynthesis and metabolism0.33
    Synthesis of 15-eicosatetraenoic acid derivatives0.00
    Synthesis of 12-eicosatetraenoic acid derivatives0.00
    3Metabolism
    Metabolism0.38
    Metabolism of lipids and lipoproteins0.37
    4Folate Metabolism
    Folate Metabolism0.54
    5Biological oxidations
    metapathway biotransformation0.42

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    2 GeneGo (Thomson Reuters) Pathways for GPX4
        HETE and HPETE biosynthesis and metabolism
    Glutathione metabolism

    Selected BioSystems Pathways for GPX4 (see all 6)
        Selenium Metabolism and Selenoproteins
    Folate Metabolism
    Selenium Pathway
    Glutathione metabolism
    metapathway biotransformation

    3 Reactome Pathways for GPX4
        Synthesis of 15-eicosatetraenoic acid derivatives
    Synthesis of 12-eicosatetraenoic acid derivatives
    Synthesis of 5-eicosatetraenoic acids


    1 Kegg Pathway  (Kegg details for GPX4):
        Glutathione metabolism


    GPX4 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including GPX4: 
              Oxidative Stress in human mouse rat
              Nitric Oxide Signaling Pathway in human mouse rat
              Molecular Toxicology PathwayFinder 384HT in human mouse rat
              Drug Metabolism in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for GPX4

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for GPX4 (P369693 ENSP000003461034) via UniProtKB, MINT, STRING, and/or I2D (see all 92)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    OTUD5Q96G743, ENSP000001560844I2D: score=2 STRING: ENSP00000156084
    PRDX6P300413, ENSP000003420264I2D: score=1 STRING: ENSP00000342026
    PRDX2P321193, ENSP000003015224I2D: score=1 STRING: ENSP00000301522
    CRNKL1Q9BZJ03I2D: score=6 
    SLC7A9P822513I2D: score=4 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 14):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006325chromatin organization IEA--
    GO:0006644phospholipid metabolic process TAS8039723
    GO:0006749glutathione metabolic process IEA--
    GO:0006979response to oxidative stress ----
    GO:0007275multicellular organismal development IEA--

    GPX4 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for GPX4

    Selected HMDB Compounds for GPX4 (see all 11)    About this table
    CompoundSynonyms CAS #PubMed Ids
    15(S)-HETE15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (see all 17)54845-95-3--
    15(S)-HPETE(5Z,8Z,11Z,13E)-(15S)-15-Hydroperoxyicosa-5,8,11,13-tetraenoate (see all 8)70981-96-3--
    5(S)-Hydroperoxyeicosatetraenoic acid5-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid (see all 6)71774-08-8--
    5-HETE5(S)-HETE (see all 14)70608-72-9--
    5-HPETE5(S)-HPETE (see all 7)74581-83-2--
    Glutathione5-L-Glutamyl-L-cysteinylglycine (see all 32)70-18-8--
    Hydrogen peroxideHydrogen peroxide (see all 44)7722-84-1--
    Oxidized glutathioneGSSG (see all 4)27025-41-8--
    Seleniumselenium (see all 5)7782-49-2--
    Selenocysteine(2R)-2-amino-3-selanylpropanoate (see all 8)3614-08-2--

    1 DrugBank Compound for GPX4    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Glutathione5-L-Glutamyl-L-cysteinylglycine (see all 5)70-18-8targetcofactor17516241 17431126 17404033 17503194 17562293

    Selected Novoseek inferred chemical compound relationships for GPX4 gene (see all 51)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    malic acid, diethyl ester 87.2 4 10506188 (1), 9442035 (1)
    selenocysteine 83.1 6 16764674 (1), 17634480 (1), 1778506 (1), 9370288 (1) (see all 5)
    selenium 82.2 74 17052796 (4), 14642406 (3), 9914487 (3), 9285063 (2) (see all 36)
    cholesterol hydroperoxide 79.2 2 8679629 (1)
    selenite 73.1 12 17150329 (2), 17052796 (1), 12431787 (1), 14642406 (1) (see all 7)
    lipid hydroperoxide 71.6 11 15256721 (3), 19769463 (2), 15577264 (2), 10560610 (1)
    linoleic acid hydroperoxide 71.2 2 7851631 (2)
    selenophosphate 69.2 2 9359853 (1)
    12-hpete 68.7 6 17997296 (1), 11115402 (1)
    13-hydroperoxyoctadecadienoic acid 65.7 2 12218361 (1)



    GPX4 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for GPX4 gene (3 alternative transcripts): 
    NM_001039847.2  NM_001039848.2  NM_002085.4  

    Unigene Cluster for GPX4:

    Glutathione peroxidase 4
    Hs.433951  [show with all ESTs]
    Unigene Representative Sequence: BG115861
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000354171(uc021umf.1 uc021umg.1 uc021umh.1) ENST00000589115
    ENST00000589115 ENST00000589115 ENST00000593032 ENST00000588919 ENST00000588919
    ENST00000585362 ENST00000587648 ENST00000592940 ENST00000585480 ENST00000587932

    Congresses - knowledge worth sharing:
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat GPX4 using miScript Target Protectors
    3 qRT-PCR Assays for microRNAs that regulate GPX4:
    hsa-miR-188-3p hsa-miR-346 hsa-miR-3667-3p
    SwitchGear 3'UTR luciferase reporter plasmidGPX4 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for GPX4
    Predesigned siRNA for gene silencing in human, mouse, rat GPX4
    Clone
    Products:
         
    OriGene clones in human, mouse for GPX4 (see all 20)
    OriGene ORF clones in mouse, rat for GPX4
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): GPX4 (NM_001039848)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for GPX4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GPX4
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for GPX4
    OriGene qSTAR qPCR primer pairs in human, mouse for GPX4
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat GPX4
      QuantiTect SYBR Green Assays in human, mouse, rat GPX4
      QuantiFast Probe-based Assays in human, mouse, rat GPX4

    Additional mRNA sequence: 

    AK225859.1 BC010157.2 BC011836.2 BC021567.1 BC022071.1 BC032695.1 BC039849.1 BC046163.1 
    BC058329.1 X71973.1 

    Selected DOTS entries (see all 25):

    DT.100638890  DT.455124  DT.95105474  DT.100867363  DT.100688494  DT.70103777  DT.95171936  DT.100867364 
    DT.91766252  DT.95171922  DT.97862488  DT.100848200  DT.86847533  DT.100045145  DT.121442151  DT.91766251 
    DT.95171920  DT.100637854  DT.91651583  DT.91766253  DT.91766256  DT.95171921  DT.95208957  DT.97862487 

    Selected AceView cDNA sequences (see all 870):

    BQ949677 BU736383 BQ671005 BP871059 BM694334 BQ575399 BU527251 AI372846 
    BP337414 BI831112 BC039849 BC021567 CA419186 BU163677 BG476519 BU541572 
    BQ574850 BI831783 BU845044 AA398796 BQ680655 BQ690090 BQ050795 H27624 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for GPX4 (see all 7)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b · 7c ^ 8a · 8b · 8c
    SP1:                    -     -                                   -           -                                 
    SP2:                    -     -                                   -           -                 -               
    SP3:                    -     -                                               -                                 
    SP4:                    -     -                                   -     -     -                                 
    SP5:                                                              -                                             


    ECgene alternative splicing isoforms for GPX4

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    GPX4 expression in normal human tissues (normalized intensities)      GPX4 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GCCTGCTGGG
    GPX4 Expression
    About this image


    GPX4 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 19) fully expand
     
     Brain (Nervous System)
             Cerebral Cortex
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Colon (Gastrointestinal Tract)
     
     Uterus (Reproductive System)
     
     Thyroid (Endocrine System)
    GPX4 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    GPX4 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.433951

    UniProtKB/Swiss-Prot: GPX4_HUMAN, P36969
    Tissue specificity: Present primarily in testis

        Pathway & Disease-focused RT2 Profiler PCR Arrays including GPX4: 
              Oxidative Stress in human mouse rat
              Nitric Oxide Signaling Pathway in human mouse rat
              Molecular Toxicology PathwayFinder 384HT in human mouse rat
              Drug Metabolism in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for GPX4
    OriGene qSTAR qPCR primer pairs in human, mouse for GPX4
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat GPX4
    QuantiTect SYBR Green Assays in human, mouse, rat GPX4
    QuantiFast Probe-based Assays in human, mouse, rat GPX4
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPX4

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for GPX4 gene from Selected species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Gpx41 , 5 glutathione peroxidase 41, 5 83.42(n)1
    88.11(a)1
      10 (39.72 cM)5
    6252491  NM_001037741.21  NP_001032830.21 
     800471665 
    lizard
    (Anolis carolinensis)
    Reptilia GPX46
    glutathione peroxidase 4
    74(a)
    1 ↔ 1
    AAWZ02041081(2345-4258)
    African clawed frog
    (Xenopus laevis)
    Amphibia 480278212   -- 74.4(n)    48027821 
    zebrafish
    (Danio rerio)
    Actinopterygii gpx4b2 glutathione peroxidase 4b 75.25(n)   352929  AY216591.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG120133 glutathione peroxidase 44(a)   63D1   --
    worm
    (Caenorhabditis elegans)
    Secernentea F26E4.123
    T09A12.21
    glutathione peroxidase3
    T09A12.21
    48(a)
    (best of 3)3
    50.1(n)1
    45.4(a)1
      I(9788403-9789394)3
    1883131  NM_001028197.41  NP_001023368.11 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes GPX21 GPX2 50.54(n)
    43.14(a)
      852546   NP_009803.3 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons GPX71 GPX7 54.47(n)
    51.63(a)
      829316  NM_119337.2  NP_194915.2 
    rice
    (Oryza sativa)
    Liliopsida Os.48332 Oryza sativa (japonica cultivar-group) cDNA clone0 more 77.85(n)    AK105165.1 


    ENSEMBL Gene Tree for GPX4 (if available)
    TreeFam Gene Tree for GPX4 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for GPX4 gene
    GPX62  GPX52  GPX22  GPX72  GPX12  GPX32  GPX82  
    3 SIMAP similar genes for GPX4 using alignment to 8 protein entries:     GPX4_HUMAN (see all proteins):
    GPX7    GPX8    GPX2

    GPX4 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for GPX4 (see all 215)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1496025181,2
    --1101969(+) CTGGGC/TGGGAG 2 -- us2k10--------
    rs7572281,2
    C,F,O,A,H--1101992(+) AACAGG/ACTTAT 2 -- us2k139Minor allele frequency- A:0.42NA EA MN NS WA CSA 5094
    rs354066801,2
    C,F--1102013(+) GATTCG/AGCCAG 2 -- us2k18Minor allele frequency- A:0.03MN NA WA 530
    rs1444165101,2
    --1102054(+) CTGCCA/GCCAAA 2 -- us2k10--------
    rs359668551,2
    C,F--1102082(+) GTGCTG/ACATCT 2 -- us2k18Minor allele frequency- A:0.03MN NA WA 530
    rs7572291,2
    C,F,A,H--1102114(+) GTGGCC/GGGGGC 2 -- us2k117Minor allele frequency- G:0.43NS MN WA NA CSA EA 913
    rs348947241,2
    F--1102118(+) CSGGGG/ACAGGC 2 -- us2k15Minor allele frequency- A:0.01MN 408
    rs7572301,2
    C,F,A,H--1102131(+) ATAGCG/ACCCCC 2 -- us2k117Minor allele frequency- A:0.43NS MN WA NA CSA EA 925
    rs45881101,2
    C,F,A,H--1102139(+) CCCCAG/AGTCAA 2 -- us2k117Minor allele frequency- A:0.43NS MN WA NA CSA EA 933
    rs37461661,2
    C,F,A,H--1102175(-) ACTGAA/GTATTT 2 -- us2k118Minor allele frequency- G:0.43NS MN WA NA CSA EA 939

    HapMap Linkage Disequilibrium report for GPX4 (1103936 - 1106787 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for GPX4 (see all 26):    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv470102CNV Loss18288195
    dgv3579n71CNV Loss21882294
    nsv910292CNV Loss21882294
    dgv3555n71CNV Loss21882294
    dgv3574n71CNV Loss21882294
    dgv3552n71CNV Loss21882294
    dgv3584n71CNV Loss21882294
    dgv3575n71CNV Loss21882294
    nsv828366CNV Loss20364138
    dgv3581n71CNV Loss21882294

    Human Gene Mutation Database (HGMD): GPX4
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing GPX4
    DNA2.0 Custom Variant and Variant Library Synthesis for GPX4

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 138322    OMIM disorders: --

    Selected diseases for GPX4 (see all 48):    About MalaCards
    spermatic cord torsion    keshan disease    glanders    toxic encephalopathy
    cerebral palsy    phenylketonuria    mastitis    infertility
    male infertility    acute pancreatitis    lung cancer susceptibility    brain disease
    lung adenocarcinoma    fanconi's anemia    burkitt's lymphoma    hypercholesterolemia
    essential hypertension    hyperglycemia    down syndrome    vascular disease

    12 diseases from the University of Copenhagen DISEASES database for GPX4:
    Vascular disease     Diabetes mellitus     Toxic encephalopathy     Heart disease
    Keshan disease     Cancer     Neurodegenerative disease     Brain disease
    Hyperglycemia     Kidney failure     Lung disease     Liver disease

    GPX4 for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    Selected Novoseek inferred disease relationships for GPX4 gene (see all 12)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    selenium deficiency 76.5 3 10464096 (1), 17937612 (1), 9914487 (1)
    infertility male 59.9 7 20104259 (1), 12193409 (1), 19783653 (1), 17937612 (1) (see all 5)
    leukemia l1210 51.9 4 7779866 (1), 8342949 (1)
    cell damage 19.7 4 11295525 (2), 9285063 (1)
    infertility 19.7 7 12606444 (1), 15577264 (1), 17937612 (1), 12695510 (1) (see all 5)
    carcinoma squamous cell 10.6 9 10428483 (2), 12958179 (2), 12218361 (1), 10882192 (1) (see all 5)
    cancer 8.42 14 17431126 (3), 15280629 (1), 11295525 (1), 14695942 (1) (see all 7)
    leukemia 0 3 16223606 (1), 1394132 (1)
    necrosis 0 3 17688422 (1)
    inflammation 0 5 16223606 (1), 12490284 (1), 15577264 (1), 12521597 (1) (see all 5)

    Genetic Association Database (GAD): GPX4
    Human Genome Epidemiology (HuGE) Navigator: GPX4 (31 documents)

    Export disorders for GPX4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for GPX4 gene, integrated from 10 sources (see all 234):
    (articles sorted by number of sources associating them with GPX4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Genetic variations of gpx-4 and male infertility in humans. (PubMed id 12606444)1, 2, 4, 9 Maiorino M.... Flohe L. (Biol. Reprod. 2003)
    2. Cloning and sequencing of the cDNA encoding a human testis phospholipid hydroperoxide glutathione peroxidase. (PubMed id 8039723)1, 2, 3 Esworthy R.S.... Chu F.-F. (Gene 1994)
    3. Phospholipid hydroperoxide glutathione peroxidase (PHGPx) expression is downregulated in poorly differentiated breast invasive ductal carcinoma. (PubMed id 17516241)1, 7, 9 Cejas P....GonzA!lez-BarA^n M. (Free Radic. Res. 2007)
    4. Structural basis for catalytic activity and enzyme polymerization of phospholipid hydroperoxide glutathione peroxidase-4 (GPx4). (PubMed id 17630701)1, 2, 9 Scheerer P....Kuhn H. (Biochemistry 2007)
    5. Structural organization of the human selenium-dependent phospholipid hydroperoxide glutathione peroxidase gene (GPX4): chromosomal localization to 19p13.3. (PubMed id 9705830)1, 2, 9 Kelner M.J. and Montoya M.A. (Biochem. Biophys. Res. Commun. 1998)
    6. Genetic variants in selenoprotein genes increase risk of colorectal cancer. (PubMed id 20378690)1, 4, 9 MAcplan C....Hesketh J.E. (Carcinogenesis 2010)
    7. Common germline genetic variation in antioxidant defense genes and survival after diagnosis of breast cancer. (PubMed id 17634480)1, 4, 9 Udler M....Pharoah P. (J. Clin. Oncol. 2007)
    8. Dual function of the selenoprotein PHGPx during sperm maturation. (PubMed id 10464096)1, 3, 9 Ursini F....FlohAc L. (Science 1999)
    9. The human glutathione peroxidase genes GPX2, GPX3, and GPX4 map to chromosomes 14, 5, and 19, respectively. (PubMed id 8287691)1, 3, 9 Chu F.F. (Cytogenet. Cell Genet. 1994)
    10. Simultaneous genotyping of 11 non-synonymous SNPs in the 4 glutathione peroxidase genes using the multiplex single base extension method. (PubMed id 19161995)1, 4, 9 Iida R....Yasuda T. ( international journal of clinical chemistry 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 2879 HGNC: 4556 AceView: GPX4 Ensembl:ENSG00000167468 euGenes: HUgn2879
    ECgene: GPX4 Kegg: 2879 H-InvDB: GPX4

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for GPX4 Pharmacogenomics, SNPs, Pathways
    NIEHS-SNPshttp://egp.gs.washington.edu/data/gpx4/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for GPX4 gene:
    Search GeneIP for patents involving GPX4

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     Browse Small Molecules at EMD Millipore
     Browse Purified and Recombinant Proteins at EMD Millipore
     Browse Kits and Assays available from EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of GPX4
     
     EMD Millipore genomic analysis products

      
     Antibodies for GPX4 (Glutathione Peroxidase 4/GPX4)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     DNA Damage/Repair Kits/Reagents for GPX4 (Glutathione Peroxidase 4/GPX4)   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for GPX4   OriGene RNAi products in human, mouse, rat for GPX4  
     OriGene qPCR primer pairs and template standards for GPX4   OriGene Protein Over-expression Lysate for GPX4  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for GPX4  
     OriGene qSTAR qPCR primer pairs in human, mouse for GPX4   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for GPX4   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for GPX4   OriGene Custom Protein Services for GPX4  

     
     
     Block miRNA regulation of human, mouse, rat GPX4 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing GPX4
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat GPX4 Predesigned siRNA for gene silencing in human, mouse, rat GPX4
     QuantiFast Probe-based Assays in human, mouse, rat GPX4 QuantiTect SYBR Green Assays in human, mouse, rat GPX4
     PCR Arrays including human, mouse, rat GPX4 Search Chromatin IP Primers for GPX4
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat GPX4  GeneGlobe Interaction Network for GPX4
     Regulatory tfbs in GPX4 promoter
     GenScript Custom Purified and Recombinant Proteins Services for GPX4 GenScript cDNA clones with any tag delivered in your preferred vector for GPX4
     GenScript Custom Assay Services for GPX4 GenScript Custom overexpressing Cell Line Services for GPX4
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Search Tocris compounds for GPX4
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Proteins for GPX4
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     GPX4 antibodies
     GPX4 proteins
     GPX4 lysates
     Antibodies for GPX4
     See all of Abcam's Antibodies, Kits and Proteins for GPX4
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for GPX4
     Antibodies for GPX4
     ELISAs for GPX4
     CLIAs for GPX4



     Browse ESI BIO Cell Lines and PureStem Progenitors for GPX4
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPX4
     SwitchGear 3'UTR luciferase reporter plasmids for GPX4
     SwitchGear Promoter luciferase reporter plasmids for GPX4
     ThermoFisher Antibody for GPX4
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GPX4
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for GPX4
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for GPX4
     LSBio Antibodies in human, mouse, rat for GPX4
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      GPX4 gene at Home site.
    Version: 3.12.136 22 July 2014
    hostname: 356980-web2.xennexinc.com index build: 126 solr: 1.4