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GPR85 Gene

protein-coding   GIFtS: 53

GC07M112507
G protein-coupled receptor 85
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FLJ50993 2
FLJ51159 2
FLJ99433 2
SREB 2
SREB2 1, 2, 3, 5
Descriptions
G protein-coupled receptor 85 2
Super conserved receptor expressed in brain 2 2, 3
seven transmembrane helix receptor 2
External Ids
HGNC: 45361
Entrez Gene: 543292
UniProtKB: P608933
Ensembl: ENSG000001646047
Search outside databases for aliases for GPR85 gene

Previous GC identifers: GC07M111205 GC07M112267 GC07M112280 GC07M112314

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for GPR85:
Members of the G protein-coupled receptor (GPCR) family, such as GPR85, have a similar structure
characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli, such as
neurotransmitters, hormones, or light, induces an intracellular signaling cascade mediated by
heterotrimeric GTP-binding proteins, or G proteins (Matsumoto et al., 2000 [PubMed
10833454]).[supplied by OMIM]

UniProtKB/Swiss-Prot: GPR85_HUMAN, P60893
Function: Orphan receptor

Gene Wiki entry for GPR85

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the GPR85 gene  

Entrez Gene cytogenetic band: 7q31   Ensembl cytogenetic band:  7q31.1   HGNC cytogenetic band: 7q31

GPR85 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M112507:     (about GC identifiers)

Start:
112,507,704 bp from pter
End:
112,515,069 bp from pter
Size:
7,366 bases
Orientation:
minus strand

1 alternative location:
Chr7-,CRA_TCAG 112,088,951-112,096,319     
RefSeq DNA sequence:
NC_000007.12  NT_007933.14  NT_079596.2  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: GPR85_HUMAN, P60893 (See protein sequence)
Recommended Name: Probable G-protein coupled receptor 85  
Size: 370 amino acids; 41995 Da
Subcellular location: Cell membrane; Multi-pass membrane protein (By similarity)
Secondary accessions: Q9JHI6 Q9NPD1

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (4 alternative transcripts): 
    NP_001139737.1  NP_001139738.1  NP_001139739.1  NP_061843.3  


    ENSEMBL proteins: 
    ENSP00000297146 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (GPCR GPR85)
    Human Recombinant Proteins from Abnova (GPR85)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886 plasma membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for GPR85: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
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    Antibodies from Abcam (GPCR GPR85), each with their AbpromiseSM
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    Novus Biologicals Antibodies for GPR85

    Assays for GPR85: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR017452 GPCR_Rhodpsn_supfam
     IPR000276 7TM_GPCR_Rhodpsn


       GeneDecks  GPR85 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P60893

    ProtoNet protein and cluster: P60893

    UniProtKB/Swiss-Prot: GPR85_HUMAN, P60893
    Similarity: Belongs to the G-protein coupled receptor 1 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (GPR85)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (GPR85)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018970

                  Applied Biosystems Silencer® siRNAs for GPR85

                  Sigma-Aldrich siRNA and siRNA Panels for GPR85  
                         Sigma-Aldrich shRNA Panels and shRNA for GPR85  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for GPR85
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018970
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018970
                                     untagged cDNA clone in CMV expression vector: NM_018970 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_018970

    UniProtKB/Swiss-Prot: GPR85_HUMAN, P60893
    Function: Orphan receptor

    3 MGI mutant phenotypes (inferred from 1 allele(MGI details for Gpr85):

    behavior/neurologicalgrowth/sizenervous system

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004872 receptor activity IEA--
    GO:0004930 G-protein coupled receptor activity TAS10833454
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/37 Interacting proteins for GPR85 (ENSP000002971463) via UniProtKB, MINT, and/or STRING (see all 37 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SCAPENSP000002655653STRING (score=.993)
    SREBF1ENSP000003480693STRING (score=.992)
    INSIG1ENSP000003400103STRING (score=.984)
    MED15ENSP000002632053STRING (score=.972)
    INSENSP000003707313STRING (score=.97)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007165 signal transduction TAS10833454
    GO:0007186 G-protein coupled receptor protein signaling pathway IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for GPR85

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (GPR85)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (GPR85)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018970

                  Sigma-Aldrich siRNA and siRNA Panels for GPR85  
                         Sigma-Aldrich shRNA Panels and shRNA for GPR85  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_018970  

    REFSEQ mRNAs for GPR85 gene (4 alternative transcripts): 

    NM_001146265.1   NM_001146266.1   NM_001146267.1   NM_018970.6   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_018970  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018970
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018970
                                     untagged cDNA clone in CMV expression vector: NM_018970 

    Additional cDNA sequence: 

    AB040800.1 AF250237.2 AK297464.1 AK302769.1 AK309392.1 AK312587.1 AL161959.1 BC030577.1 
    CR609708.1 

    5 DOTS entries:

    DT.70100926  DT.307158  DT.121106438  DT.121106435  DT.95146749 

    24/34 AceView cDNA sequences (see all 34 ):

    CR609708 BI915894 AW513647 AL540052 BG719824 NM_018970 AL161959 T06908 
    AW148538 AI673342 AI910962 AI631153 BC030577 BQ771598 BX453573 AF250237 
    AB040800 CD246165 AA732890 BX431250 AI200800 AL540051 AA883367 N62306 

    highest scoring ESTs for GPR85:

    AI631153 AF250237 AI200800 AI673342 AI910962 AL540052 BC030577 BG719824 AA332611 AA732890 

    Unigene Cluster for GPR85:

    G protein-coupled receptor 85
    Hs.152009  [show with all ESTs]
    Unigene Representative Sequence: NM_018970


    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for GPR85 (see all 6 )

    ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4a · 4b · 4c · 4d · 4e · 4f
    SP1:              -     -                                             
    SP2:              -     -                                             
    SP3:                    -                                             
    SP4:                    -                                             
    SP5:                    -     -                                       

    About this scheme

    ECgene alternative splicing isoforms for GPR85

    1 Ensembl transcript including schematic representation:
    ENST00000297146  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    GPR85 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for GPR85

    1 / 2 / 3

    5 probe-sets matching GPR85 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    83293_at2, 3 U95-E 1 0.94 1.00 1.00 1.00 AI631153 1.00 1.00 1.00 1

    219898_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018970 0.60 1.00 0.82 1

    234303_s_at2, 3 U133-B 1 1.00 1.00 -- -- AL161959 0.60 1.00 0.82 1

    219898_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    234303_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GTTTGAAACT

    SOURCE GeneReport for Unigene cluster: Hs.152009

    UniProtKB/Swiss-Prot: GPR85_HUMAN, P60893
    Tissue specificity: Highly expressed in brain and testis. Lower levels in small intestine, placenta
    and spleen. In brain regions, detected in all regions tested, but somewhat lower levels in the
    corpus callosum, medulla and spinal cord

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for GPR85 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    GPR851   -- G protein-coupled receptor 85 97.21(n)
    100(a)
    482410  XM_539527.2  XP_539527.2 
    chimpanzee
    (Pan troglodytes)
    GPR851   -- G protein-coupled receptor 85 99.73(n)
    100(a)
    463664  XM_519318.2  XP_519318.2 
    rat
    (Rattus norvegicus)
    Gpr851   -- G protein-coupled receptor 85 95.59(n)
    100(a)
    64020  NM_022254.3  NP_071590.1 
    mouse
    (Mus musculus)
    Gpr851, 5 65
    G protein-coupled receptor 851, 5 94.95(n)1
    100(a)1
    644501  NM_145066.41  NP_659503.11 
     AF2544165  AI8369985  (see all 11)
    chicken
    (Gallus gallus)
    GPR851   -- G protein-coupled receptor 85 91.17(n)
    99.73(a)
    417775  XM_416019.2  XP_416019.2 
    About this table        Species with no ortholog for GPR85

    ENSEMBL Gene Tree for GPR85
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for GPR85 gene
    GPR1732  GPR272  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/56 NCBI SNPs in GPR85 are shown (see all 56 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 72)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 7 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs21409131,2
    A,C,F,H,O112515289(+) TTTTTA/CGAAAA 1 -- ng3112Minor allele frequency- C:0.09EU EA WA NA 2396
    rs15901541,2
    A,C,F,H112516418(+) CTTTCA/CAAATT 1 -- ng3111Minor allele frequency- C:0.26NA EU EA WA 968
    rs15750121,2
    A,C,F,H112512031(+) ATAAGG/TATACA 1 -- ut51 ese313Minor allele frequency- T:0.19NS MN EU EA WA NA 948
    rs15816881,2
    A,C,F,H112510329(+) CAATAT/CACTGC 1 -- ut3113Minor allele frequency- C:0.12MN EU EA WA NA 2596
    rs15750131,2
    A,C,F,H112516146(+) GCTTAG/ATTGGT 1 -- ng317Minor allele frequency- A:0.25EU EA WA NA 558
    rs8702661,2
    C,F,H112510757(-) TGCAAT/AAGTTC 1 -- ut3116Minor allele frequency- A:0.04MN EU EA WA NA 1572
    rs22933691,2
    C,F,H112508060(-) TTTGCT/GTGGAT 1 -- ut31 ese38Minor allele frequency- G:0.14EA NA 1970
    rs127058461,2
    C,F,H112516243(+) ATACAA/GCAAAA 1 -- ng314Minor allele frequency- G:0.18EU EA WA 420
    rs22558011,2
    A,C,F112509116(+) AATAAA/GCAAAA 1 -- ut31 ese33Minor allele frequency- G:0.18NA 118
    rs22558111,2
    C,F112509432(+) GGAATA/GTAGTA 1 -- ut31 ese33Minor allele frequency- G:0.23NA 134
    About this table

    HapMap Linkage Disequilibrium images for GPR85 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 605188

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/16 PubMed articles for GPR85 gene (see all 16 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 54329 HGNC: 4536 AceView: GPR85 Ensembl:ENSG00000164604 euGenes: HUgn54329
    ECgene: GPR85 H-InvDB: GPR85
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for GPR85:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for GPR85 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for GPR85 Browse small molecules at Sigma-Aldrich
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     Antibodies for GPR85

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