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GPR82 Gene

protein-coding   GIFtS: 49
GCID: GC0XP041583

G Protein-Coupled Receptor 82

  See related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
G Protein-Coupled Receptor 821 2
Probable G-Protein Coupled Receptor 822

External Ids:    HGNC: 45331   Entrez Gene: 271972   Ensembl: ENSG000001716577   OMIM: 3007485   UniProtKB: Q96P673   

Export aliases for GPR82 gene to outside databases

Previous GC identifers: GC0XP039612 GC0XP039832 GC0XP040428 GC0XP040614 GC0XP041339 GC0XP041468 GC0XP039314


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for GPR82 Gene:
The protein encoded by this gene is an orphan G protein-coupled receptor of unknown function. The encoded protein
is a member of a family of proteins that contain seven transmembrane domains and transduce extracellular signals
through heterotrimeric G proteins. (provided by RefSeq, Sep 2011)

GeneCards Summary for GPR82 Gene:
GPR82 (G protein-coupled receptor 82) is a protein-coding gene. Diseases associated with GPR82 include mental retardation. GO annotations related to this gene include G-protein coupled receptor activity. An important paralog of this gene is P2RY13.

UniProtKB/Swiss-Prot: GPR82_HUMAN, Q96P67
Function: Orphan receptor

Gene Wiki entry for GPR82 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000023.11  NC_018934.2  NT_079573.5  
Regulatory elements:
   Regulatory transcription factor binding sites in the GPR82 gene promoter:
         GR   AML1a   AP-1   POU3F2 (N-Oct-5b)   POU3F2 (N-Oct-5a)   POU3F2   Evi-1   E47   Hand1   Meis-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidGPR82 promoter sequence
   Search Chromatin IP Primers for GPR82

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat GPR82


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xp11.4   Ensembl cytogenetic band:  Xp11.4   HGNC cytogenetic band: Xp11.4

GPR82 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GPR82 gene location

GeneLoc information about chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP041583:  view genomic region     (about GC identifiers)

Start:
41,583,408 bp from pter      End:
41,589,388 bp from pter
Size:
5,981 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: GPR82_HUMAN, Q96P67 (See protein sequence)
Recommended Name: Probable G-protein coupled receptor 82  
Size: 336 amino acids; 38409 Da
Secondary accessions: Q5VT13

Explore the universe of human proteins at neXtProt for GPR82: NX_Q96P67

Explore proteomics data for GPR82 at MOPED

Post-translational modifications: 

  • Glycosylation2 at Asn3, Asn4
  • Modification sites at PhosphoSitePlus

  • See GPR82 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_543007.1  
    ENSEMBL proteins: 
     ENSP00000303549  

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    GPCRAO: GPCR / Class A : Orphans

    IUPHAR Guide to PHARMACOLOGY protein family classification: GPR82
    Class A Orphans

    2 InterPro protein domains:
     IPR017452 GPCR_Rhodpsn_7TM
     IPR000276 GPCR_Rhodpsn

    Graphical View of Domain Structure for InterPro Entry Q96P67

    ProtoNet protein and cluster: Q96P67

    UniProtKB/Swiss-Prot: GPR82_HUMAN, Q96P67
    Similarity: Belongs to the G-protein coupled receptor 1 family


    GPR82 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: GPR82_HUMAN, Q96P67
    Function: Orphan receptor

         Gene Ontology (GO): 1 molecular function term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004930G-protein coupled receptor activity IEA--
         
    GPR82 for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for GPR82:
     Increased gamma-H2AX phosphory 

         1 MGI mutant phenotype (inferred from 1 allele(MGI details for Gpr82):
     normal 

    GPR82 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Gpr82tm1Lex for GPR82

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for GPR82
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    miRTarBase miRNAs that target GPR82:
    hsa-mir-335-5p (MIRT017157)

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    Selected qRT-PCR Assays for microRNAs that regulate GPR82 (see all 13):
    hsa-miR-579 hsa-miR-1290 hsa-miR-1283 hsa-miR-876-5p hsa-miR-340 hsa-miR-548aa hsa-miR-888* hsa-miR-664
    SwitchGear 3'UTR luciferase reporter plasmidGPR82 3' UTR sequence
    Inhib. RNA
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    GPR82_HUMAN, Q96P67: Cell membrane; Multi-pass membrane protein
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    plasma membrane5
    endoplasmic reticulum1

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886plasma membrane IEA--
    GO:0016021integral component of membrane IEA--

    GPR82 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Pathway & Disease-focused RT2 Profiler PCR Array including GPR82: 
              G Protein Coupled Receptors 384HT in human mouse rat

    Interactions:

        Search GeneGlobe Interaction Network for GPR82

    Gene Ontology (GO): 1 biological process term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007186G-protein coupled receptor signaling pathway ----

    GPR82 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for GPR82



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for GPR82 gene: 
    NM_080817.4  

    Unigene Cluster for GPR82:

    G protein-coupled receptor 82
    Hs.567457  [show with all ESTs]
    Unigene Representative Sequence: NM_080817
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000302548(uc004dfu.1 uc004dft.3) ENST00000497180
    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate GPR82 (see all 13):
    hsa-miR-579 hsa-miR-1290 hsa-miR-1283 hsa-miR-876-5p hsa-miR-340 hsa-miR-548aa hsa-miR-888* hsa-miR-664
    SwitchGear 3'UTR luciferase reporter plasmidGPR82 3' UTR sequence
    Inhib. RNA
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      QuantiTect SYBR Green Assays in human, mouse, rat GPR82
      QuantiFast Probe-based Assays in human, mouse, rat GPR82

    Additional mRNA sequence: 

    AJ319076.1 AK122626.1 AK313702.1 BC070180.1 

    3 DOTS entries:

    DT.100666997  DT.100000981  DT.40118022 

    14 AceView cDNA sequences:

    AJ319076 CB985246 BX503542 AA923209 AW768359 NM_080817 AL832460 BX438968 
    BC070180 AK122626 AA282067 AI819140 CB955669 BF664534 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    GPR82 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TCACAGCTTG
    GPR82 Expression
    About this image

    GPR82 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    GPR82 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.567457
        Pathway & Disease-focused RT2 Profiler PCR Array including GPR82: 
              G Protein Coupled Receptors 384HT in human mouse rat

    Primer
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPR82

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for GPR82 gene from Selected species (see all 11)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Gpr821 , 5 G protein-coupled receptor 821, 5 86.34(n)1
    82.57(a)1
      X (8.59 cM)5
    3192001  NM_175669.31  NP_783600.11 
     136613635 
    chicken
    (Gallus gallus)
    Aves GPR821 G protein-coupled receptor 82 60.06(n)
    48.17(a)
      101750076  XM_004934603.1  XP_004934660.1 
    lizard
    (Anolis carolinensis)
    Reptilia --
    Uncharacterized protein
    18(a)
    many ↔ many
    GL343202.1(2086373-2088440)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia gpr821 G protein-coupled receptor 82 53.84(n)
    40.3(a)
      101735236  XM_004911775.1  XP_004911832.1 
    zebrafish
    (Danio rerio)
    Actinopterygii LOC1018835021 probable G-protein coupled receptor 82-like 47.54(n)
    35.42(a)
      101883502  XM_005167792.1  XP_005167849.1 


    ENSEMBL Gene Tree for GPR82 (if available)
    TreeFam Gene Tree for GPR82 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for GPR82 gene
    P2RY132  GPR1712  GPR872  GPR342  P2RY142  P2RY122  
    1 SIMAP similar gene for GPR82 using alignment to 1 protein entry:     GPR82_HUMAN:
    GPCR

    GPR82 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    1 SNPs for GPR82    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr X posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1939211041,2
    --untested141505299(+) ATGGGG/TAGTTG 2 G V mis10--------

    HapMap Linkage Disequilibrium report for GPR82 (41583408 - 41589388 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for GPR82:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv33720CNV Loss17666407
    nsv523134CNV Gain19592680

    Locus Specific Mutation Databases (LSDB): GPR82

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing GPR82
    DNA2.0 Custom Variant and Variant Library Synthesis for GPR82

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 300748    OMIM disorders: --

    1 disease for GPR82:    
    About MalaCards
    mental retardation


    GPR82 for disorders           About GeneDecksing


    Export disorders for GPR82 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for GPR82 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with GPR82)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Discovery and mapping of ten novel G protein-coupled receptor genes. (PubMed id 11574155)1, 2, 3 Lee D.K.... O'Dowd B.F. (Gene 2001)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    3. Identification of G protein-coupled receptor genes from the human genome sequence. (PubMed id 12044878)1, 2 Takeda S.... Mitaku S. (FEBS Lett. 2002)
    4. A systematic, large-scale resequencing screen of X-chromosome coding exons in mental retardation. (PubMed id 19377476)1 Tarpey P.S.... Stratton M.R. (Nat. Genet. 2009)
    5. The DNA sequence of the human X chromosome. (PubMed id 15772651)2 Ross M.T.... Bentley D.R. (Nature 2005)
    6. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (Nat. Genet. 2004)
    7. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 27197 HGNC: 4533 AceView: GPR82 Ensembl:ENSG00000171657 euGenes: HUgn27197
    ECgene: GPR82 H-InvDB: GPR82

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for GPR82 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for GPR82 gene:
    Search GeneIP for patents involving GPR82

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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