GPR65 Gene
protein-coding GIFtS: 52
GCID: GC14P088471
|
|
G protein-coupled receptor 65
| |
Aliases
for GPR65 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| G Protein-Coupled Receptor 651 2 | | TDAG81 2 3 5 | | HTDAG81 | | G-Protein Coupled Receptor 652 3 | | T-Cell Death-Associated Gene 8 Protein2 3 | | Psychosine Receptor2 |
Export aliases for GPR65 gene to outside databasesPrevious GC identifers: GC14P085976 GC14P082288 GC14P086461 GC14P087541 GC14P068641 |
Summaries
for GPR65 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: PSYR_HUMAN, Q8IYL9Function: Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a rolein activation-induced cell death or differentiation of T-cells
Gene Wiki entry for GPR65
|
Genomic Views
for GPR65 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000014.8 NC_018925.1 NT_026437.12
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the GPR65 gene promoter:
Max1 ER-alpha C/EBPbeta NF-1 FOXO3 C/EBPalpha FOXO3b FAC1 FOXO3a c-Myc
Other transcription factors
Search SABiosciences Chromatin IP Primers for GPR65
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GPR65 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 14q31-q32.1 Ensembl cytogenetic band: 14q31.3 HGNC cytogenetic band: 14q31-q32.1GPR65 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 14 GeneLoc Exon Structure GeneLoc location for GC14P088471: view genomic region
(about GC identifiers)
Start:
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88,471,468 bp from pter |
End:
|
88,481,155 bp from pter |
Size:
|
9,688 bases |
Orientation:
|
plus strand |
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Proteins
for GPR65 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: PSYR_HUMAN, Q8IYL9 (See
protein sequence)Recommended Name: Psychosine receptor Size: 337 amino acids; 39333 Da
Subcellular location: Cell membrane; Multi-pass membrane protein (By similarity)
Secondary accessions: O75819Explore the universe of human proteins at neXtProt for GPR65: NX_Q8IYL9
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8IYL9
GPR65 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_003599.2
ENSEMBL proteins: ENSP00000267549 Reactome Protein details: Q8IYL9
Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
GPR65 for ontologies About GeneDecksing
GPR65 Antibody Products:
Assay Products for GPR65: |
Protein
Domains /
Families
for GPR65 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
GPR65 for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q8IYL9ProtoNet protein and cluster: Q8IYL9 1 Blocks protein family: IPB005464 Psychosine receptor signature
UniProtKB/Swiss-Prot: PSYR_HUMAN, Q8IYL9Similarity: Belongs to the G-protein coupled receptor 1 family |
Function
for GPR65 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: PSYR_HUMAN, Q8IYL9Function: Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a rolein activation-induced cell death or differentiation of T-cells Genatlas biochemistry entry for GPR65:G protein coupled receptor 65,predominantly expressed in lymphoid tissues,G protein coupled receptor superfamily
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GPR65
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPR65 |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004930 | G-protein coupled receptor activity |
IDA | 15618224 |
GPR65 for ontologies About GeneDecksing
Animal Models:
1 MGI mutant phenotype (inferred from 2 alleles ) (MGI details for Gpr65):
GPR65 for phenotypes About GeneDecksing
|
Pathways & Interactions
for GPR65 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Class A/1 (Rhodopsin-like receptors) | | | 2 | Signaling by GPCR | | | 3 | G alpha (q) signalling events | |
Pathway sources
See GeneCards unified pathways
Show all pathways
1  BioSystems Pathway for GPR65
5/7 
Reactome Pathways for GPR65 (see all 7)
GPR65 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for GPR65
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/123 Interacting proteins for GPR65 (ENSP000002675494) via UniProtKB, MINT, STRING, and/or I2D (see all 123)About this table
Gene Ontology (GO): 5/9 biological process terms (GO ID links to tree view) (see all 9): About this table
GPR65 for ontologies About GeneDecksing
|
Drugs & Compounds
for GPR65 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
GPR65 for compounds About GeneDecksing
 Browse Tocris compounds for GPR65
2 Novoseek chemical compound relationships for GPR65 gene About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| psychosine |
84.4 |
1 |
11309421 (1) |
| lysophosphatidylcholine |
60.4 |
2 |
15280385 (1) |
Search CenterWatch for drugs/clinical trials and news about GPR65 / PSYR
|
Transcripts
for GPR65 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for GPR65 gene: NM_003608.3 Unigene Cluster for GPR65: G protein-coupled receptor 65 Hs.443243 [show with all ESTs]Unigene Representative Sequence: NM_0036081 Ensembl transcript including schematic representation, and UCSC links where relevant: ENST00000267549(uc001xvv.3)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for GPR65 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GPR65
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: GPR65 (NM_003608) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for GPR65 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GPR65 |
Additional cDNA sequence: AK313797.1 BC035633.1 BC071715.1 EU883574.1 3 DOTS entries: DT.205257 DT.40228234 DT.95270486 24/33 AceView cDNA sequences (see all 33): BE326632 BI767444 CD246175 BI823897 AI445416 BI819962 BI824101 AA298823 BI836708 BC071715 BC035633 BI518633 AI376916 BI909137 CD522351 AI017452 BX093977 NM_003608 AI707599 CB266201 BI908385 AV716718 AI344626 BI764698
GeneLoc Exon Structure
|
Expression
for GPR65 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| GPR65 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TGAGGGGACT
About this image
See GPR65 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for GPR65
SOURCE GeneReport for Unigene cluster: Hs.443243
UniProtKB/Swiss-Prot: PSYR_HUMAN, Q8IYL9Tissue specificity: Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta andperipheral blood leukocytes
SABiosciences Expression via Pathway-Focused PCR Array including GPR65:
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for GPR65
Browse OriGene validated miRNA SYBR primer pairs
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Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPR65 |
Orthologs
for GPR65 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for GPR65 gene from 3/10 species (see all 10) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
GPR651 |
G protein-coupled receptor 65 |
63.26(n)
57.43(a) |
|
423395 XM_421305.3 XP_421305.1 |
lizard
(Anolis carolinensis) |
Reptilia |
GPR656 |
-- |
46(a) |
1 ↔ 1 |
1(13960651-13961727) |
zebrafish
(Danio rerio) |
Actinopterygii |
si:ch211-51o12.56 |
si:ch211-51o12.5 |
31(a) |
1 ↔ 1 |
17(2588745-2595004) |
ENSEMBL Gene Tree for GPR65 (if available)
TreeFam Gene Tree for GPR65 (if available) |
Paralogs
for GPR65 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for GPR65 gene
- GPR42 FFAR32 GPR682 P2RY82 F2RL12 FFAR22 F2RL22 GPR1322
- FFAR12 GPR422 F2RL32 F2R2
12 SIMAP similar genes for GPR65 using alignment to 2 protein entries: PSYR_HUMAN (see all proteins):GPR68 GPR4 LPAR4 GPR17 LPAR6 P2RY5
CCR4 P2RY1 PTAFR GPR132 BDKRB2 G2A
GPR65 for paralogs About GeneDecksing
|
Genomic Variants
for GPR65 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 14 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for GPR65 (88471468 - 88481155 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for GPR65: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing GPR65 |
|
Disorders
/ Diseases
for GPR65 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
GPR65 for disorders About GeneDecksing
OMIM gene information: 604620
OMIM disorders: --
3  diseases for GPR65: About MalaCardskrabbe disease crohn's disease peritonitis
1 disease from the University of Copenhagen DISEASES database for GPR65:Krabbe disease 3 Novoseek disease relationships for GPR65 gene About this table
Export disorders for GPR65 gene to outside databases
|
Publications
for GPR65 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for GPR65 gene, integrated from 9 sources (see all 22):
(articles sorted by number of sources associating them with GPR65) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Cloning, characterization, and mapping of human homolog of mouse T- cell death-associated gene. (PubMed id 9655242)1, 2, 3, 9 Kyaw H.... Li Y. (1998)
- Identification of a molecular target of psychosine and its role in globoid cell formation. (PubMed id 11309421)1, 2, 9 Im D.-S.... Lynch K.R. (2001)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- The DNA sequence and analysis of human chromosome 14. (PubMed id 12508121)1, 2 Heilig R.... Weissenbach J. (2003)
- Identification of T cell death-associated gene 8 (TDAG8) as a novel acid sensing G-protein-coupled receptor. (PubMed id 15618224)1, 9 Ishii S....Shimizu T. (2005)
- G protein-coupled receptors GPR4 and TDAG8 are oncogenic and overexpressed in human cancers. (PubMed id 15221007)1, 9 Sin W.C....Yang J. (2004)
- Differential proton sensitivity of related G protein-coupled receptors T cell death-associated gene 8 and G2A expressed in immune cells. (PubMed id 15665078)1, 9 Radu C.G....Witte O.N. (2005)
- Acidosis promotes Bcl-2 family-mediated evasion of apo ptosis: involvement of acid-sensing G protein-coupled receptor Gpr65 signaling t o Mek/Erk. (PubMed id 22685289)1 Ryder C....Distelhorst C.W. (2012)
- Activation of T cell death-associated gene 8 regulates the cytokine production of T cells and macrophages in vitro. (PubMed id 22445881)1 Onozawa Y....Oda T. (2012)
- Genetic risk and a primary role for cell-mediated immu ne mechanisms in multiple sclerosis. (PubMed id 21833088)1 ....Compston A. (2011)
|
External Searches for GPR65 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing GPR65 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing GPR65 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing GPR65 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for GPR65 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for GPR65 gene:
Search GeneIP for patents involving GPR65
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for GPR65 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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