GPR18 Gene
protein-coding GIFtS: 53
GCID: GC13M099906
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G protein-coupled receptor 18
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Aliases
for GPR18 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| G Protein-Coupled Receptor 181 2 | | NAGly Receptor2 3 | | N-Arachidonyl Glycine Receptor2 | | GPCRW3 | | G-Protein Coupled Receptor 183 |
Export aliases for GPR18 gene to outside databasesPrevious GC identifers: GC13M098266 GC13M094294 GC13M098743 GC13M097604 GC13M098704 GC13M080501 |
Summaries
for GPR18 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: GPR18_HUMAN, Q14330Function: Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibitadenylyl cyclase. May contribute to regulation of the immune system
Gene Wiki entry for GPR18
|
Genomic Views
for GPR18 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000013.10 NC_018924.1 NT_009952.14
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the GPR18 gene promoter:
AhR Elk-1 AML1a p53 C/EBPalpha Arnt RORalpha1 PPAR-gamma1 SEF-1 (1) PPAR-gamma2
Other transcription factors
Search SABiosciences Chromatin IP Primers for GPR18
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GPR18 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 13q32 Ensembl cytogenetic band: 13q32.3 HGNC cytogenetic band: 13q32GPR18 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 13 GeneLoc Exon Structure GeneLoc location for GC13M099906: view genomic region
(about GC identifiers)
Start:
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99,906,967 bp from pter |
End:
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99,913,998 bp from pter |
Size:
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7,032 bases |
Orientation:
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minus strand |
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Proteins
for GPR18 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: GPR18_HUMAN, Q14330 (See
protein sequence)Recommended Name: N-arachidonyl glycine receptor Size: 331 amino acids; 38134 Da
Subcellular location: Cell membrane; Multi-pass membrane protein
Sequence caution: Sequence=AAG44671.1; Type=Frameshift; Positions=78, 81, 129;
Secondary accessions: Q6GTM3 Q96HI6 Q9H2L2Explore the universe of human proteins at neXtProt for GPR18: NX_Q14330
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q14330
GPR18 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001091670.1 NP_005283.1
ENSEMBL proteins: ENSP00000343428 ENSP00000401611 ENSP00000380613 ENSP00000380610 Reactome Protein details: Q14330
Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
GPR18 for ontologies About GeneDecksing
GPR18 Antibody Products:
Assay Products for GPR18: |
Protein
Domains /
Families
for GPR18 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
GPR18 for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q14330ProtoNet protein and cluster: Q14330 UniProtKB/Swiss-Prot: GPR18_HUMAN, Q14330Similarity: Belongs to the G-protein coupled receptor 1 family |
Function
for GPR18 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: GPR18_HUMAN, Q14330Function: Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibitadenylyl cyclase. May contribute to regulation of the immune system Genatlas biochemistry entry for GPR18:G protein coupled receptor 18,abundantly expressed in testis and spleen
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GPR18 (see all 2)
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPR18 |
Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004872 | receptor activity |
-- | -- | | GO:0004930 | G-protein coupled receptor activity |
NAS | 9205118 | | GO:0045028 | G-protein coupled purinergic nucleotide receptor activity |
IEA | -- |
GPR18 for ontologies About GeneDecksing
|
Pathways & Interactions
for GPR18 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Class A/1 (Rhodopsin-like receptors) | | | 2 | Signaling by GPCR | | | 3 | GPCRs, Other | |
Pathway sources
See GeneCards unified pathways
Show all pathways
2  BioSystems Pathways for GPR18
5/6 
Reactome Pathways for GPR18 (see all 6)
GPR18 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for GPR18
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/143 Interacting proteins for GPR18 (ENSP000003434284) via UniProtKB, MINT, STRING, and/or I2D (see all 143)About this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0007186 | G-protein coupled receptor signaling pathway |
NAS | 9205118 |
GPR18 for ontologies About GeneDecksing
|
Drugs & Compounds
for GPR18 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for GPR18
1 HMDB Compound for GPR18 About this table
1 DrugBank Compound for GPR18 About this table
Search CenterWatch for drugs/clinical trials and news about GPR18
|
Transcripts
for GPR18 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for GPR18 gene (2 alternative transcripts): NM_001098200.1 NM_005292.3 Unigene Cluster for GPR18: G protein-coupled receptor 18 Hs.741589 [show with all ESTs]Unigene Representative Sequence: BC0669274 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000340807(uc010afv.3) ENST00000416594 ENST00000397473(uc001voe.4)
ENST00000397470
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for GPR18 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GPR18 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: GPR18 (NM_004951) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for GPR18 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GPR18 |
Additional cDNA sequence: AF261135.1 AF339781.1 BC008569.1 BC050646.1 BC066927.1 BT009907.1 JQ308176.1 4 DOTS entries: DT.40117945 DT.95157497 DT.86857234 DT.75119902 24/34 AceView cDNA sequences (see all 34): BI828900 AA258898 BC008569 AF339781 BX102267 BC050646 BC066927 NM_005292 BF794361 AW574811 AW968257 CN835171 BU182977 BI770379 AA705077 BT009907 AA258793 AW403055 AW406896 BF796745 AW574862 AF261135 AI208357 BG501756
GeneLoc Exon Structure
2 Alternative Splicing Database (ASD) splice patterns (SP) for GPR18 About this scheme
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3a | · | 3b |
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| SP1: | | | | | | | | | | | |
| SP2: | | | | | - | | | | | |
ECgene alternative splicing isoforms for GPR18
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Expression
for GPR18 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| GPR18 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CGCAGAAAAA
About this image
See GPR18 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for GPR18
SOURCE GeneReport for Unigene cluster: Hs.741589
UniProtKB/Swiss-Prot: GPR18_HUMAN, Q14330Tissue specificity: Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well asCD19-positive B-cells
SABiosciences Expression via Pathway-Focused PCR Array including GPR18:
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for GPR18
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat GPR18 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat GPR18 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GPR18 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPR18 |
Orthologs
for GPR18 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for GPR18 gene from 4/10 species (see all 10) About this table
ENSEMBL Gene Tree for GPR18 (if available)
TreeFam Gene Tree for GPR18 (if available) |
Paralogs
for GPR18 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for GPR18 gene
- GPR1412
18/33 SIMAP similar genes for GPR18 using alignment to 2 protein entries: GPR18_HUMAN (see all proteins)
(see all similar genes):F2RL1 AGTR1 F2RL2 OPRK1 GPR1 P2RY6
C3AR1 CCR3 CCR5 CX3CR1 CCR7 P2RY14
CCR2 C5AR1 GALR1 CCRL1 RXFP3 GPR17
GPR18 for paralogs About GeneDecksing
|
Genomic Variants
for GPR18 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 13 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for GPR18 (99906967 - 99913998 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for GPR18: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing GPR18 |
|
Disorders
/ Diseases
for GPR18 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
GPR18 for disorders About GeneDecksing
OMIM gene information: 602042
OMIM disorders: --
4  diseases for GPR18: About MalaCardsbipolar disorder peritonitis melanoma schizophrenia
Export disorders for GPR18 gene to outside databases
|
Publications
for GPR18 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for GPR18 gene, integrated from 9 sources (see all 11):
(articles sorted by number of sources associating them with GPR18) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Cloning and chromosomal localization of a gene (GPR18) encoding a novel seven transmembrane receptor highly expressed in spleen and testis. (PubMed id 9205118)1, 2, 3 Gantz I.... Yamada T. (1997)
- Identification of N-arachidonylglycine as the endogenous ligand for orphan G-protein-coupled receptor GPR18. (PubMed id 16844083)1, 2 Kohno M....Yasukawa M. (2006)
- The DNA sequence and analysis of human chromosome 13. (PubMed id 15057823)1, 2 Dunham A.... Ross M.T. (2004)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Quantitative expression profiling of G-protein-couple d receptors (GPCRs) in metastatic melanoma: the constitutively active orphan GP CR GPR18 as novel drug target. (PubMed id 20880198)1 Qin Y....Osanto S. (2011)
- EBI2, GPR18 and GPR17--three structurally related, bu t biologically distinct 7TM receptors. (PubMed id 21261596)1 NA...Rosenkilde M.M. (2011)
- Dissociation of heterotrimeric g proteins in cells. (PubMed id 18577758)1 Lambert N.A. (2008)
- Drugs, their targets and the nature and number of dru g targets. (PubMed id 17016423)7 Imming P....Meyer A. (2006)
- How many drug targets are there? (PubMed id 17139284)7 Overington J.P....Hopkins A.L. (2006)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
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External Searches for GPR18 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing GPR18 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing GPR18 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing GPR18 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for GPR18 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for GPR18 gene:
Search GeneIP for patents involving GPR18
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for GPR18 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat GPR18 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing GPR18 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GPR18 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat GPR18 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GPR18 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat GPR18 |
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 | | | GPR18 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPR18 |
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| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GPR18 |
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