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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

GPR114 Gene

protein-coding   GIFtS: 48
GCID: GC16P057576

G Protein-Coupled Receptor 114

  Search for GPR114
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
G Protein-Coupled Receptor 1141 2
PGR272 3
G-Protein Coupled Receptor PGR272 3
G Protein-Coupled Receptor PGR272
G-Protein Coupled Receptor 1142
Probable G-Protein Coupled Receptor 1142

External Ids:    HGNC: 190101   Entrez Gene: 2211882   Ensembl: ENSG000001596187   UniProtKB: Q8IZF43   

Export aliases for GPR114 gene to outside databases

Previous GC identifers: GC16P057636 GC16P057311 GC16P057351 GC16P056134 GC16P043444


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

GeneCards Summary for GPR114 Gene: 
GPR114 (G protein-coupled receptor 114) is a protein-coding gene. GO annotations related to this gene include G-protein coupled receptor activity. An important paralog of this gene is GPR97.

UniProtKB/Swiss-Prot: GP114_HUMAN, Q8IZF4
Function: Orphan receptor

Gene Wiki entry for GPR114 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000016.9  NT_010498.15  NC_018927.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the GPR114 gene promoter:
         c-Fos   Spz1   AP-1   MIF-1   AP-2gamma   AP-2beta   SEF-1 (1)   c-Jun   Pax-4a   AP-2alphaA   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidGPR114 promoter sequence
   Search SABiosciences Chromatin IP Primers for GPR114

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GPR114


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q21   Ensembl cytogenetic band:  16q21   HGNC cytogenetic band: 16q13

GPR114 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GPR114 gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16P057576:  view genomic region     (about GC identifiers)

Start:
57,576,333 bp from pter      End:
57,625,593 bp from pter
Size:
49,261 bases      Orientation:
plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: GP114_HUMAN, Q8IZF4 (See protein sequence)
Recommended Name: Probable G-protein coupled receptor 114 precursor  
Size: 528 amino acids; 59000 Da
Subcellular location: Cell membrane; Multi-pass membrane protein
Sequence caution: Sequence=AAN46670.1; Type=Erroneous gene model prediction; Sequence=BAD18748.1; Type=Erroneous
initiation;
Secondary accessions: B3KXZ5 Q6ZMH7 Q6ZML4 Q86SL8 Q8IZ14

Explore the universe of human proteins at neXtProt for GPR114: NX_Q8IZF4

Explore proteomics data for GPR114 at MOPED 

GPR114 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

GPR114 Protein Expression

REFSEQ proteins: NP_722579.1  
ENSEMBL proteins: 
 ENSP00000290823   ENSP00000342981  

Human Recombinant Protein Products for GPR114: 
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Browse ProSpec Recombinant Proteins
Cloud-Clone Corp. Proteins for GPR114 

Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005886plasma membrane IEA--
GO:0016020membrane ----
GO:0016021integral to membrane IEA--

GPR114 for ontologies           About GeneDecksing



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Cloud-Clone Corp. CLIAs for GPR114


(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section
HGNC Gene Families: 
GPCRBO: GPCR / Class B : Orphans

IUPHAR Guide to PHARMACOLOGY protein family classification: GPR114 
Adhesion Class GPCRs

4 InterPro protein domains:
 IPR003910 GPCR_2_orphan_rcpt_GPR56
 IPR000832 GPCR_2_secretin-like
 IPR017981 GPCR_2-like
 IPR000203 GPS_dom

Graphical View of Domain Structure for InterPro Entry Q8IZF4

ProtoNet protein and cluster: Q8IZF4

3 Blocks protein domains:
IPB000203 GPS domain
IPB000832 G-protein coupled receptors family 2 (secretin-like)
IPB003910 GPR56 orphan receptor signature


UniProtKB/Swiss-Prot: GP114_HUMAN, Q8IZF4
Similarity: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily
Similarity: Contains 1 GPS domain


GPR114 for domains           About GeneDecksing


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
About This Section

Molecular Function:

     UniProtKB/Swiss-Prot Summary: GP114_HUMAN, Q8IZF4
Function: Orphan receptor

     Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0004888transmembrane signaling receptor activity ----
GO:0004930G-protein coupled receptor activity IEA--
     
GPR114 for ontologies           About GeneDecksing


Phenotypes:
     1 GenomeRNAi human phenotype for GPR114:
 Increased HPV18 LCR reporter a 

     1 MGI phenotypic allele for Gpr114 (no phenotypes)

GPR114 for phenotypes           About GeneDecksing

Animal Models:
   inGenious Targeting Laboratory - Custom generated mouse model solutions for GPR114 
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miRNA
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SwitchGear 3'UTR luciferase reporter plasmidGPR114 3' UTR sequence
Inhib. RNA
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In Situ Assay
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Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPR114


(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
About This Section




Interactions:

    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for GPR114

Gene Ontology (GO): 3 biological process terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0007166cell surface receptor signaling pathway ----
GO:0007186G-protein coupled receptor signaling pathway TAS15203201
GO:0007218neuropeptide signaling pathway IEA--

GPR114 for ontologies           About GeneDecksing



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section
Browse Small Molecules at EMD Millipore
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for GPR114 (GP114)

Search CenterWatch for drugs/clinical trials and news about GPR114 / GP114

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for GPR114 gene: 
NM_153837.1  

Unigene Cluster for GPR114:

G protein-coupled receptor 114
Hs.187884  [show with all ESTs]
Unigene Representative Sequence: AK160368
6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000349457(uc002elx.4 uc010vhr.2) ENST00000564983 ENST00000394361
ENST00000564607 ENST00000340339(uc002ely.3) ENST00000569839
miRNA
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4 QIAGEN miScript miRNA Assays for microRNAs that regulate GPR114:
hsa-miR-125a-5p hsa-miR-149 hsa-miR-125b hsa-miR-4319
SwitchGear 3'UTR luciferase reporter plasmidGPR114 3' UTR sequence
Inhib. RNA
Products:
     
Browse for Gene Knock-down Tools from EMD Millipore
OriGene RNAi products in human, mouse, rat for GPR114
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat GPR114
Clone
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Sirion Biotech Customized lentivirus for stable overexpression of GPR114 
                     Customized lentivirus expression plasmids for stable overexpression of GPR114 
Primer
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OriGene qPCR primer pairs and template standards for GPR114
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  QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat GPR114
  QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GPR114

Additional mRNA sequence: 

AK128298.1 AK160368.1 AK172765.1 AK292783.1 AK301357.1 AK303661.1 AY140956.1 AY255609.1 
AY358447.1 BC032401.1 

5 DOTS entries:

DT.200337  DT.40268865  DT.101980593  DT.70101965  DT.95204004 

24/34 AceView cDNA sequences (see all 34):

AA338682 BF001154 AY255609 CK825716 BC032401 AA865753 NM_153837 BG757374 
AA338603 AW402064 AY358447 CA443499 AK128298 AW139813 AY140956 AI355086 
CK825717 AI138727 AL704772 AK160368 AK172765 AI088225 BI518500 BM193863 

GeneLoc Exon Structure

5/6 Alternative Splicing Database (ASD) splice patterns (SP) for GPR114 (see all 6)    About this scheme

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15
SP1:              -                       -                                                                           
SP2:                                      -                                                                           
SP3:              -                       -                                                                           
SP4:                                      -                                                     -     -     -         
SP5:              -                                                                                                   


ECgene alternative splicing isoforms for GPR114

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

GPR114 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: CACCAGCTGG
GPR114 Expression
About this image


See GPR114 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for GPR114

SOURCE GeneReport for Unigene cluster: Hs.187884
    SABiosciences Expression via Pathway-Focused PCR Array including GPR114: 
          G Protein Coupled Receptors 384HT in human mouse rat

Primer
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In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GPR114

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of chordates.

Orthologs for GPR114 gene from 3/9 species (see all 9)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Gpr1141 , 5 G protein-coupled receptor 1141, 5 75.48(n)1
70.88(a)1
  8 (47.03 cM)5
3820451  NM_001145972.11  NP_001139444.11 
 949236945 
chicken
(Gallus gallus)
Aves GPR1141 G protein-coupled receptor 114 61.44(n)
52.37(a)
  415634  XM_414000.3  XP_414000.3 
zebrafish
(Danio rerio)
Actinopterygii gpr566
G protein-coupled receptor 56
19(a)
1 → many
7(30006746-30032840)


ENSEMBL Gene Tree for GPR114 (if available)
TreeFam Gene Tree for GPR114 (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
Paralogs for GPR114 gene
GPR972  CD972  ELTD12  LPHN22  LPHN12  EMR12  LPHN32  EMR22  
GPR1332  GPR1122  GPR1442  GPR562  EMR32  GPR1262  GPR642  
6 SIMAP similar genes for GPR114 using alignment to 1 protein entry:     GP114_HUMAN:
GPR126    GPR64    KPG_006    GPR97    GPR112    GPR56

GPR114 for paralogs           About GeneDecksing



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

10/867 SNPs in GPR114 are shown (see all 867)    About this table     
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 16 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs1174366071,2
F--57574602(+) GCAGGG/AAGGTG 1 -- us2k11Minor allele frequency- A:0.01NA 120
rs1429443541,2
--57574744(+) GTTGCC/TCAAGC 1 -- us2k10--------
rs1510793061,2
--57574790(+) CTCCAC/TCTCCC 1 -- us2k10--------
rs597619471,2
C,F--57574828(+) CTCCCG/AAGTAC 1 -- us2k12Minor allele frequency- A:0.12CSA WA 120
rs1407794781,2
--57574834(+) AGTACA/CTAGAA 1 -- us2k10--------
rs1442794331,2
--57574908(+) ATGTTA/GCCCAG 1 -- us2k10--------
rs80446421,2
C--57574943(+) AGTAAT/ACTGCC 1 -- us2k13Minor allele frequency- A:0.16NA EA 242
rs735450751,2
C,F--57574961(+) ACCTCC/ACAAAG 1 -- us2k14Minor allele frequency- A:0.24WA CSA 124
rs47848231,2
C,F,A--57575075(+) GTAACA/GGGCAC 1 -- us2k1 tfbs320Minor allele frequency- G:0.40NA EA WA CSA 880
rs713871731,2
C,F--57575129(+) AGACCC/AAGGCT 1 -- us2k12Minor allele frequency- A:0.50NA 4

HapMap Linkage Disequilibrium report for GPR114 (57576333 - 57625593 bp)

Structural Variations
     Database of Genomic Variants (DGV) 6 variations for GPR114:    About this table     
Variant IDTypeSubtypePubMed ID
esv2667803CNV Deletion23128226
nsv906728CNV Loss21882294
nsv906727CNV Loss21882294
nsv833245CNV Loss17160897
esv33007CNV Loss17666407
nsv906725CNV Loss21882294

SABiosciences Cancer Mutation PCR Assays
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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

GPR114 for disorders           About MalaCards

GPR114 for disorders           About GeneDecksing

Genetic Association Database (GAD): GPR114
Human Genome Epidemiology (HuGE) Navigator: GPR114 (1 document)

Export disorders for GPR114 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
About This Section

PubMed articles for GPR114 gene integrated from 9 sources:
(articles sorted by number of sources associating them with GPR114)
    Utopia: connect your pdf to the dynamic
world of online information

  1. Novel human G protein-coupled receptors with long N-terminals containing GPS domains and Ser/Thr-rich regions. (PubMed id 12435584)1, 2, 3 Fredriksson R.... Schioeth H.B. (2002)
  2. Genome-wide association study of vitamin D concentrat ions in Hispanic Americans: the IRAS family study. (PubMed id 20600896)1, 4 Engelman C.D....Norris J.M. (2010)
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
  5. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)1, 2 Clark H.F.... Gray A.M. (2003)
  6. The G protein-coupled receptor repertoires of human and mouse. (PubMed id 12679517)1, 2 Vassilatis D.K.... Gaitanaris G.A. (2003)
  7. The human and mouse repertoire of the adhesion family of G-protein- coupled receptors. (PubMed id 15203201)1 Bjarnadottir T.K.... Schioeth H.B. (2004)
  8. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 221188 HGNC: 19010 AceView: GPR114 Ensembl:ENSG00000159618 euGenes: HUgn221188
ECgene: GPR114 H-InvDB: GPR114

(According to HUGE)
About This Section
  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
NameDescription
PharmGKB entry for GPR114 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
About This Section
Patent Information for GPR114 gene:
Search GeneIP for patents involving GPR114

GeneCards and IP:
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(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 3 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

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