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GPBAR1 Gene

protein-coding   GIFtS: 51

GC02P218832
G protein-coupled bile acid receptor 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
BG37 1, 2, 3, 5
GPCR 1, 2
GPCR19 1, 2
GPR131 1, 2
M-BAR 1, 2, 3
MGC40597 1, 2
TGR5 1, 2, 3
hBG37 3
hGPCR19 3
Descriptions
G protein-coupled bile acid receptor 1 2
G-protein coupled bile acid receptor BG37 2
Membrane-type receptor for bile acids 2, 3
membrane bile acid receptor 2
External Ids
HGNC: 196801
Entrez Gene: 1513062
UniProtKB: Q8TDU63
Ensembl: ENSG000001799217
Search outside databases for aliases for GPBAR1 gene

Previous GC identifers: GC02P217847 GC02P219090 GC02P219328 GC02P218949

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for GPBAR1:
This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme
functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with
bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling
pathway, and internalization of the receptor. The receptor is implicated in the suppression of
macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing
results in multiple transcript variants encoding the same protein. [provided by RefSeq]

UniProtKB/Swiss-Prot: GPBAR_HUMAN, Q8TDU6
Function: Receptor for bile acid. Bile acid-binding induces its internalization, activation of
extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the
suppression of macrophage functions by bile acids

Gene Wiki entry for GPBAR1 (G_protein-coupled_bile_acid_receptor)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the GPBAR1 gene  

Entrez Gene cytogenetic band: 2q35   Ensembl cytogenetic band:  2q35   HGNC cytogenetic band: 2q35

GPBAR1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P218832:     (about GC identifiers)

Start:
218,833,983 bp from pter
End:
218,836,826 bp from pter
Size:
2,844 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_005403.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: GPBAR_HUMAN, Q8TDU6 (See protein sequence)
Recommended Name: G-protein coupled bile acid receptor 1  
Size: 330 amino acids; 35248 Da
Subcellular location: Cell membrane; Multi-pass membrane protein

REFSEQ proteins (3 alternative transcripts): 
NP_001070659.1  NP_001070662.1  NP_733800.1  

Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (GPCR TGR5)
Human Recombinant Proteins from Abnova (GPBAR1)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005886 plasma membrane IEA--
GO:0016021 integral to membrane IEA--
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Antibodies for GPBAR1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for GPBAR1
R&D Systems Antibodies for GPBAR1
Antibodies from Abcam (GPCR TGR5), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Novus Biologicals Antibodies for GPBAR1

Assays for GPBAR1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

2 InterPro domains/families:
 IPR017452 GPCR_Rhodpsn_supfam
 IPR000276 7TM_GPCR_Rhodpsn


   GeneDecks  GPBAR1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q8TDU6

ProtoNet protein and cluster: Q8TDU6

UniProtKB/Swiss-Prot: GPBAR_HUMAN, Q8TDU6
Similarity: Belongs to the G-protein coupled receptor 1 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (GPBAR1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (GPBAR1)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_170699

              Applied Biosystems Silencer® siRNAs for GPBAR1

              Sigma-Aldrich siRNA and siRNA Panels for GPBAR1  
                     Sigma-Aldrich shRNA Panels and shRNA for GPBAR1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for GPBAR1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001077191
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001077191
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_001077191 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_170699

UniProtKB/Swiss-Prot: GPBAR_HUMAN, Q8TDU6
Function: Receptor for bile acid. Bile acid-binding induces its internalization, activation of
extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the
suppression of macrophage functions by bile acids

5 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Gpbar1):

adipose tissueendocrine/exocrine glandgrowth/sizehomeostasis/metabolismliver/biliary system

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004872 receptor activity IEA--
GO:0004930 G-protein coupled receptor activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


 Gene Network CentralTM Interacting Genes and Proteins Network for  GPBAR1 

  --

2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007165 signal transduction IEA--
GO:0007186 G-protein coupled receptor protein signaling pathway IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for GPBAR1
8 Novoseek chemical compound relationships for GPBAR1 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
tyrosine 53.37 19 11502566 (1), 16620963 (1), 17274683 (1), 15456867 (1) (see all 19)
threonine 44.19 5 17995450 (1), 11399057 (1), 15375171 (1), 11641411 (1) (see all 5)
sphingosine 33.16 2 16164409 (1), 14623109 (1)
deoxycholic acid 22.22 2 17214962 (2)
lysine 4.56 1 15713849 (1)
phospholipid 0.00 2 15110780 (1), 18931035 (1)
lipid 0.00 3 15556605 (1), 17070497 (1), 19418628 (1)
calcium 0.00 11 19035847 (4), 15598880 (1), 16110482 (1), 8734454 (1) (see all 5)
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (GPBAR1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (GPBAR1)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_170699

              Sigma-Aldrich siRNA and siRNA Panels for GPBAR1  
                     Sigma-Aldrich shRNA Panels and shRNA for GPBAR1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001077191  NM_001077194  NM_170699  

REFSEQ mRNAs for GPBAR1 gene (3 alternative transcripts): 

NM_001077191.1   NM_001077194.1   NM_170699.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001077191  NM_001077194  NM_170699  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001077191
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001077191
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_001077191 

Additional cDNA sequence: 

AB086170.1 AB089307.1 AK122658.1 AK122660.1 BC033625.1 CR604439.1 CR625402.1 

6 DOTS entries:

DT.95261319  DT.100721867  DT.100747103  DT.101981636  DT.100747104  DT.100768004 

24/32 AceView cDNA sequences (see all 32 ):

CR625402 CR604439 AA595679 BX369250 NM_170699 CD370746 AB086170 BI834364 
AK122660 AK122658 BX366596 BC033625 BI199349 BX365038 BX094903 BF312517 
BG698138 BI833418 AB089307 BI522077 BF207290 BX366595 AI391439 AA100813 

highest scoring ESTs for GPBAR1:

AA595679 AB086170 AB089307 AK122658 AK122660 BC033625 BE819289 BF207290 BF312517 BI199349 

Unigene Cluster for GPBAR1:

G protein-coupled bile acid receptor 1
Hs.160954  [show with all ESTs]
Unigene Representative Sequence: NM_001077191


GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for GPBAR1

ExUns: 1 ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b · 4c
SP1:        -     -     -     -     -               
SP2:                                                
SP3:                          -     -               
SP4:                          -                     

About this scheme

ECgene alternative splicing isoforms for GPBAR1

1 Ensembl transcript including schematic representation:
ENST00000354867  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

GPBAR1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for GPBAR1

1 / 2 / 3

2 probe-sets matching GPBAR1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

81914_at2, 3 U95-D 1 1.00 1.00 1.00 1.00 AI391439 0.60 1.00 0.82 1

1552501_a_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: GGGCTGGGCG

SOURCE GeneReport for Unigene cluster: Hs.160954

UniProtKB/Swiss-Prot: GPBAR_HUMAN, Q8TDU6
Tissue specificity: Ubiquitously expressed. Expressed at higher level in spleen and placenta.
Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach,
duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum
and liver, but not in esophagus and rectum

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for GPBAR1 gene from 5/6 species (see all 6 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
GPBAR11   -- G protein-coupled bile acid receptor 1 85.86(n)
88.48(a)
488521  XM_545641.2  XP_545641.1 
chimpanzee
(Pan troglodytes)
GPBAR11   -- G protein-coupled bile acid receptor 1 99.29(n)
99.39(a)
741111  XM_001155488.1  XP_001155488.1 
cow
(Bos taurus)
GPBAR11   -- G protein-coupled bile acid receptor 1 86.42(n)
87.23(a)
317756  NM_175049.2  NP_778219.1 
rat
(Rattus norvegicus)
Gpbar11   -- G protein-coupled bile acid receptor 1 81.97(n)
82.37(a)
338443  NM_177936.1  NP_808797.1 
mouse
(Mus musculus)
Gpbar11, 5 1 (40.40 cM)5
G protein-coupled bile acid receptor 11, 5 83.13(n)1
83.84(a)1
2272891  NM_174985.11  NP_778150.11 
 AB0861715  AB0893085  (see all 7)
About this table        Species with no ortholog for GPBAR1

ENSEMBL Gene Tree for GPBAR1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/21 NCBI SNPs in GPBAR1 are shown (see all 21 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 37)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 2 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs37318591,2
C,F,H218832467(+) TGCCCA/GACCAG 3 -- ng51 ese38Minor allele frequency- G:0.46EA NA EU WA 2050
rs102036421,2
C,F,H218832014(+) ATTCCG/ATGCCA 3 -- ng514Minor allele frequency- A:0.11EU EA WA 382
rs115548251,2
C218834054(+) CCATGC/TACTTG 3 -- ut510--------
rs15678691,2
A,C218834980(-) TGAACA/GTGGAG 3 -- int10--------
rs37959811,2
H218834334(+) TTTGTC/GGCTGT 3 -- int14Minor allele frequency- G:0.00EU EA WA 418
--
rs58386881,2
--218835896(+) CATTG-/CCCACA 3 F/F fra10--------
--
rs115548231,2
--218834034(+) GTCCCC/TACTGG 3 -- ut510--------
--
rs130033341,2
--218833166(+) AACTAA/TGGGCC 3 -- ng510--------
--
rs605104021,2
--218833403(+) ACCACA/GACCTT 3 -- ng510--------
--
rs101902921,2
--218835107(+) CTGCTC/TACTGA 3 -- ut51 int10--------
About this table

HapMap Linkage Disequilibrium images for GPBAR1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 610147

4 Novoseek disease relationships for GPBAR1 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
albinism ocular 54.11 1 10471510 (1)
tumors 0.95 1 17178880 (1)
cancer 0.00 4 17178880 (3), 19361994 (1)
neurodegenerative diseases 0.00 1 16805430 (1)
About this table

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/171 PubMed articles for GPBAR1 gene (see all 171 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 151306 HGNC: 19680 AceView: GPBAR1 Ensembl:ENSG00000179921 euGenes: HUgn151306
ECgene: GPBAR1 H-InvDB: GPBAR1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for GPBAR1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Predesigned and custom siRNAs for GPBAR1 Antibodies for GPBAR1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for GPBAR1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Search Tocris compounds for GPBAR1




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 Antibodies for GPBAR1

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