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GOT1 Gene

protein-coding GIFtS: 66
GCID: GC10M101146

Glutamic-Oxaloacetic Transaminase 1, Soluble

(Previous names: glutamic-oxaloacetic transaminase 1, soluble (aspartate...)

See GOT1-related diseases
at

MalaCards

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
About This Section

Aliases
Glutamic-Oxaloacetic Transaminase 1, Soluble¹ ²		cCAT² ³
Aspartate Aminotransferase 1¹ ²		EC 2.6.1.1³ ⁸
Aspartate Transaminase 1¹ ²		ASTQTL1² ⁵
Cysteine Aminotransferase, Cytoplasmic² ³		Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1)¹
Cysteine Transaminase, Cytoplasmic² ³		GIG18²
Glutamate Oxaloacetate Transaminase 1² ³		Aspartate Aminotransferase, Cytoplasmic²
Transaminase A² ³		Growth-Inhibiting Protein 18²
cAspAT² ³		EC 2.6.1.3³

External Ids:	HGNC: 4432¹	Entrez Gene: 2805²	Ensembl: ENSG00000120053⁷	OMIM: 138180⁵	UniProtKB: P17174³

Export aliases for GOT1 gene to outside databases

Previous GC identifers: GC10M100049 GC10M100390 GC10M101287 GC10M100821 GC10M094783

(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for GOT1 Gene:

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and

mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and

tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. (provided by RefSeq, Jul

2008)

GeneCards Summary for GOT1 Gene:

GOT1 (glutamic-oxaloacetic transaminase 1, soluble) is a protein-coding gene. Diseases associated with GOT1 include aspartate aminotransferase, serum level of, qtl1, and exanthem. GO annotations related to this gene include pyridoxal phosphate binding and carboxylic acid binding. An important paralog of this gene is GOT2.

UniProtKB/Swiss-Prot: AATC_HUMAN, P17174

Function: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate,

the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate

in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during

development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of

mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the

action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and

neuroprotectant in the brain

Gene Wiki entry for GOT1 Gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence at NCBI GenBank:

NC_000010.10 NC_018921.2 NT_030059.14

Regulatory elements:

Regulatory transcription factor binding sites in the GOT1 gene promoter:
STAT1 STAT1beta Egr-4 STAT1alpha AREB6 Egr-2 PPAR-gamma1 FOXO1a PPAR-gamma2 FOXO1
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: GOT1 promoter sequence

Search Chromatin IP Primers for GOT1

Epigenetics:

DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat GOT1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q24.1-q25.1 Ensembl cytogenetic band: 10q24.2 HGNC cytogenetic band: 10q24.1-q25.1

GOT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GOT1 gene location

GeneLoc information about chromosome 10 GeneLoc Exon Structure

GeneLoc location for GC10M101146: view genomic region (about GC identifiers)

Start:	101,156,627 bp from pter	End:	101,190,530 bp from pter
Size:	33,904 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, ²neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, antibodies-online, and/or GeneTex,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
About This Section

UniProtKB/Swiss-Prot: AATC_HUMAN, P17174 (See protein sequence)

Recommended Name: Aspartate aminotransferase, cytoplasmic

Size: 413 amino acids; 46248 Da

Cofactor: Pyridoxal phosphate

Subunit: Homodimer

Miscellaneous: In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes

Miscellaneous: Aspartate aminotransferase activity found to be increased in cerebral spinal fluid (CSF) of

patients with Alzheimer disease (PubMed:16039064). Fetal serum levels of the enzyme in the umbilical artery and

vein are found to be significantly higher than maternal serum levels (PubMed:22633534)

1 PDB 3D structure from and Proteopedia for GOT1:

3II0 (3D)

Secondary accessions: B2R6R7 Q5VW80

Explore the universe of human proteins at neXtProt for GOT1: NX_P17174

Explore proteomics data for GOT1 at MOPED

Post-translational modifications:

Modification sites at neXtProt

Modification sites at PhosphoSitePlus

Selected DME Specific Peptides for GOT1 (P17174) (see all 21)

DPTPEQW

EKRVGGV

CAHNPTG

AGFKDIR

See GOT1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins: NP_002070.1

ENSEMBL proteins:

ENSP00000359539 ENSP00000445578

Reactome Protein details: P17174

GOT1 Human Recombinant Protein Products:

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	Novus Biologicals GOT1 Proteins Novus Biologicals GOT1 Lysates
	Sino Biological Recombinant Protein for GOT1 Browse Sino Biological Cell Lysates
	ProSpec Recombinant Protein for GOT1
	Browse Proteins at Cloud-Clone Corp.
	Search eBioscience for Proteins for GOT1
	Search GeneTex for Proteins for GOT1
	antibodies-online proteins for GOT1 (29 products)
	antibodies-online peptides for GOT1

GOT1 Antibody Products:

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	antibodies-online antibodies for GOT1 (69 products)

GOT1 Assay Products:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Assay Services for GOT1
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for GOT1
	Browse Enzo Life Sciences for kits & assays
	Browse ELISAs at Cloud-Clone Corp. Browse CLIAs at Cloud-Clone Corp.
	Search eBioscience for ELISAs for GOT1
	antibodies-online kits for GOT1 (44 products)

(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GenesLikeMe)
About This Section

5 InterPro protein domains:

IPR004838 NHTrfase_class1_PyrdxlP-BS

IPR004839 Aminotransferase_I/II

IPR015424 PyrdxlP-dep_Trfase

IPR000796 Asp_trans

IPR015421 PyrdxlP-dep_Trfase_major_sub1

Graphical View of Domain Structure for InterPro Entry P17174

ProtoNet protein and cluster: P17174

3 Blocks protein domains:

IPB000796 Aspartate aminotransferase signature

IPB004838 Aminotransferase

IPB004839 Aminotransferase

UniProtKB/Swiss-Prot: AATC_HUMAN, P17174

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family

Find genes that share domains with GOT1 About GenesLikeMe

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene, Sets of similar genes according to GenesLikeMe)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: AATC_HUMAN, P17174

Function: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate,

the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate

in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during

development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of

mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the

action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and

neuroprotectant in the brain

Catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

Catalytic activity: L-cysteine + 2-oxoglutarate = mercaptopyruvate + L-glutamate

Genatlas biochemistry entry for GOT1:

glutamic-oxaloacetic transaminase 1,soluble (aspartate aminotransferase 1)

Enzyme Numbers (IUBMB): EC 2.6.1.1¹ ² EC 2.6.1.3¹

Gene Ontology (GO): Selected molecular function terms (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004069	L-aspartate:2-oxoglutarate aminotransferase activity	TAS	--
GO:0004609	phosphatidylserine decarboxylase activity	IEA	--
GO:0008483	transaminase activity	TAS	--
GO:0030170	pyridoxal phosphate binding	IEA	--
GO:0031406	carboxylic acid binding	IEA	--

Find genes that share ontologies with GOT1 About GenesLikeMe

Phenotypes:
2 GenomeRNAi human phenotypes for GOT1:

Elongated cells

Upregulation of Wnt/beta-caten

Animal Models:

genOway: Develop your customized and physiologically relevant rodent model for GOT1

miRNA Products:		miRTarBase miRNAs that target GOT1: hsa-mir-769-5p (MIRT039193)

		Block miRNA regulation of human, mouse, rat GOT1 using miScript Target Protectors
		8 qRT-PCR Assays for microRNAs that regulate GOT1:
		hsa-miR-876-3p hsa-miR-9 hsa-miR-3122 hsa-miR-1276 hsa-miR-3913-5p hsa-miR-3151 hsa-miR-323-5p hsa-miR-1207-5p

		SwitchGear 3'UTR luciferase reporter plasmid: GOT1 3' UTR sequence

Inhib. RNA Products:		OriGene RNAi products in human, mouse, rat for GOT1
		Predesigned siRNA for gene silencing in human, mouse, rat GOT1

Gene Editing
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DNA2.0 Custom Protein Engineering Service for GOT1

Clone Products:		OriGene clones in human, mouse for GOT1 (see all 7) OriGene ORF clones in mouse, rat for GOT1 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: GOT1 (NM_002079)
		Sino Biological Human cDNA Clone for GOT1
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for GOT1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GOT1
		Addgene plasmids for GOT1

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for GOT1
		Browse ESI BIO Cell Lines and PureStem Progenitors for GOT1

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GOT1

(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene, Sets of similar genes according to GenesLikeMe)
About This Section

Subcellular locations from UniProtKB/Swiss-Prot

AATC_HUMAN, P17174: Cytoplasm

Subcellular locations from COMPARTMENTS:

Compartment	Confidence
cytosol	4
extracellular	3
mitochondrion	3
lysosome	2
nucleus	2
peroxisome	2
plasma membrane	2
vacuole	2
cytoskeleton	1
endoplasmic reticulum	1

About this image

Gene Ontology (GO): 5 cellular component terms: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005737	cytoplasm	IDA	7060339
GO:0005764	lysosome	IEA	--
GO:0005829	cytosol	TAS	--
GO:0043679	axon terminus	IEA	--
GO:0070062	extracellular vesicular exosome	IDA	19056867

Find genes that share ontologies with GOT1 About GenesLikeMe

(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GenesLikeMe, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene, Sets of similar genes according to GenesLikeMe)
About This Section

SuperPaths for GOT1 About (see all 14)

See pathways by source

Powered
by

SuperPath

Contained pathways About

Phenylalanine metabolism

Phenylalanine metabolism	0.36	Phenylalanine, tyrosine and tryptophan biosynthesis	0.00
Tyrosine metabolism	0.36	phenylalanine degradation IV	0.00
phenylalanine utilization	0.33

Glucose metabolism

	Glucose metabolism	0.44			malate-aspartate shuttle	0.00
	Gluconeogenesis	0.44

Metabolism

Metabolism

0.38

Metabolic pathways

0.38

Sulfur amino acid metabolism

Sulfur amino acid metabolism

0.35

Cysteine and methionine metabolism

0.35

Valproic Acid Pathway, Pharmacodynamics

aspartate biosynthesis

0.00

Valproic Acid Pathway, Pharmacodynamics

Find genes that share SuperPaths with GOT1 About GenesLikeMe

Pathways by source See SuperPaths
Show all pathways

Selected BioSystems Pathways for GOT1 (see all 9)

	Trans-sulfuration pathway
	malate-aspartate shuttle
	Alanine and aspartate metabolism
	Glycolysis and Gluconeogenesis
	phenylalanine degradation IV

3 Reactome Pathways for GOT1

	Gluconeogenesis
	Methionine salvage pathway
	Amino acid synthesis and interconversion (transamination)

1 PharmGKB Pathway for GOT1

Valproic Acid Pathway, Pharmacodynamics

Selected Kegg Pathways (Kegg details for GOT1) (see all 10):

	Alanine, aspartate and glutamate metabolism
	Cysteine and methionine metabolism
	Arginine and proline metabolism
	Tyrosine metabolism
	Phenylalanine metabolism

Pathway & Disease-focused RT² Profiler PCR Array including GOT1:

Amino Acid Metabolism II in human mouse rat

Interactions:

Search GeneGlobe Interaction Network for GOT1

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

Selected Interacting proteins for GOT1 (P17174^{2, 3} ENSP00000359539⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 237)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
HIST1H4A	P62805³	I2D: score=1
HIST1H4B	P62805³	I2D: score=1
HIST1H4C	P62805³	I2D: score=1
HIST1H4D	P62805³	I2D: score=1
HIST1H4E	P62805³	I2D: score=1

About this table

Gene Ontology (GO): Selected biological process terms (see all 24): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000096	sulfur amino acid metabolic process	TAS	--
GO:0005975	carbohydrate metabolic process	TAS	--
GO:0006006	glucose metabolic process	TAS	--
GO:0006094	gluconeogenesis	TAS	--
GO:0006103	2-oxoglutarate metabolic process	ISS	--

Find genes that share ontologies with GOT1 About GenesLikeMe

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, Sets of similar genes according to GenesLikeMe)
About This Section

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse compounds at ApexBio

Browse Tocris compounds for GOT1 (AATC)

Selected HMDB Compounds for GOT1 (see all 18) About this table

Compound	Synonyms	CAS #	PubMed Ids
3-Mercaptopyruvic acid	3-Mercaptopyruvate (see all 13)	2464-23-5	--
3-Sulfinoalanine	3-Sulphino-L-alanine (see all 9)	1115-65-7	--
3-Sulfinylpyruvic acid	3-Sulfinopyruvate (see all 5)	--	--
3-Sulfopyruvic acid	3-sulfopyruvate (see all 8)	98022-26-5	--
4-Hydroxy-2-oxoglutaric acid	(+/-)-2-hydroxy-4-oxopentanedioate (see all 9)	1187-99-1	--
4-Hydroxy-L-glutamic acid	4-hydroxy-L-glutamate (see all 7)	2485-33-8	--
4-Hydroxyphenylpyruvic acid	(p-Hydroxyphenyl)pyruvate (see all 25)	156-39-8	--
Cysteic acid	2-Amino-3-sulfopropionate (see all 19)	498-40-8	--
Famotidine	Amfamox (see all 83)	76824-35-6	--
L-Aspartic acid	(+)-Aspartate (see all 41)	56-84-8	--

5 DrugBank Compounds for GOT1 About this table

Compound	Synonyms	CAS #	Type	Actions	PubMed Ids
L-Aspartic Acid	(+)-Aspartic acid (see all 23)	56-84-8	target	--	17244260 17328883 17168267 17442055 17545671
Pyridoxal Phosphate	Codecarboxylase (see all 9)	54-47-7	target	activator	12167474 11752352 16925884 11340119 11888303
L-Cysteine	(R)-2-Amino-3-mercaptopropanoic acid (see all 13)	52-90-4	target	--	17139284 17016423
L-Glutamic Acid	(2S)-2-Aminopentanedioic acid (see all 15)	56-86-0	target	--	17444813
Maleic Acid	--	110-16-7	target	--	10592235

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
About This Section

REFSEQ mRNAs for GOT1 gene:

NM_002079.2

Unigene Cluster for GOT1:

Glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)

Hs.500756 [show with all ESTs]

Unigene Representative Sequence: NM_002079

4 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000370508(uc001kpr.3 uc009xwi.2) ENST00000489349 ENST00000471741

ENST00000543866

miRNA Products:		Block miRNA regulation of human, mouse, rat GOT1 using miScript Target Protectors
		8 qRT-PCR Assays for microRNAs that regulate GOT1:
		hsa-miR-876-3p hsa-miR-9 hsa-miR-3122 hsa-miR-1276 hsa-miR-3913-5p hsa-miR-3151 hsa-miR-323-5p hsa-miR-1207-5p

		SwitchGear 3'UTR luciferase reporter plasmid: GOT1 3' UTR sequence

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		GenScript: all cDNA clones in your preferred vector: GOT1 (NM_002079)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for GOT1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GOT1
		Addgene plasmids for GOT1

Primer Products:		OriGene qPCR primer pairs and template standards for GOT1 OriGene qSTAR qPCR primer pairs in human, mouse for GOT1
		Pre-validated RT² qPCR Primer Assay in human, mouse, rat GOT1
		QuantiTect SYBR Green Assays in human, mouse, rat GOT1
		QuantiFast Probe-based Assays in human, mouse, rat GOT1

Additional mRNA sequence:

AF052153.1 AK293483.1 AK301916.1 AK309174.1 AK312684.1 AY513279.1 BC000498.2 M37400.1

18 DOTS entries:

DT.95161440 DT.100880095 DT.450537 DT.100767584 DT.100880100 DT.121276203 DT.95161437 DT.100880101

DT.91766831 DT.91766816 DT.102823882 DT.121276217 DT.86843273 DT.91766828 DT.95161431 DT.97854688

DT.100743022 DT.101979725

Selected AceView cDNA sequences (see all 584):

R38726 BU557951 H98877 BM477387 AV651308 BQ574228 BM980073 F02675

CR625110 CR606661 CR621846 BQ427619 AW409837 AI365638 BU848920 BI833571

CR598904 BU615309 W87692 F10117 AW135113 AF052153 CR615083 BI912612

GeneLoc Exon Structure

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GenesLikeMe, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

GOT1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>^2/3
CGAP TAG: CACGGACACG

About this image

GOT1 expression in embryonic tissues and stem cells About this table

Data from LifeMap, the Embryonic Development and Stem Cells Database
selected tissues (see all 6) fully expand


Brain (Nervous System) fully expand to see all 5 entries
Cerebral Cortex

Neural Tube (Nervous System) fully expand to see all 3 entries
Metencephalon

Thymus (Hematopoietic System)
Thymus

Gut Tube (Gastrointestinal Tract)
Foregut

Liver (Hepatobiliary System)
Periportal Hepatocytes Liver Lobule

GOT1 Protein expression data from MOPED¹, PaxDb² and MaxQB³:

About this image

GOT1 Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.500756

Pathway & Disease-focused RT² Profiler PCR Array including GOT1:

Amino Acid Metabolism II in human mouse rat

Primer Products:		OriGene qPCR primer pairs and template standards for GOT1 OriGene qSTAR qPCR primer pairs in human, mouse for GOT1
		Pre-validated RT² qPCR Primer Assay in human, mouse, rat GOT1
		QuantiTect SYBR Green Assays in human, mouse, rat GOT1
		QuantiFast Probe-based Assays in human, mouse, rat GOT1

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GOT1

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of eukaryotes.

Orthologs for GOT1 gene from Selected species (see all 26) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Got1^{1 , 5}	glutamate oxaloacetate transaminase 1, soluble^{1, 5}	87.09(n)¹ 90.8(a)¹		19 (36.67 cM)⁵ 14718¹ NM_010324.2¹ NP_034454.2¹ 43499752⁵
chicken (Gallus gallus)	Aves	GOT1¹	glutamic-oxaloacetic transaminase 1, soluble (aspartate more	76.12(n) 80.44(a)		396261 NM_205321.1 NP_990652.1
lizard (Anolis carolinensis)	Reptilia	GOT1⁶	glutamic-oxaloacetic transaminase 1, soluble	81(a)	1 ↔ 1	3(38473516-38490723)
African clawed frog (Xenopus laevis)	Amphibia	xr406-prov²	Aspartate aminotransferase	75.29(n)		BC045269.1
zebrafish (Danio rerio)	Actinopterygii	Dr.28236²	Danio rerio cDNA clone MGC55996 IMAGE3819704, complete more	75.9(n)		BC047800.1
fruit fly (Drosophila melanogaster)	Insecta	CG8430³ Got1¹	aspartate aminotransferase³ Glutamate oxaloacetate transaminase 1¹	56(a)³ 59.27(n)¹ 57.11(a)¹		36782¹ NM_166148.1¹ NP_725534.1¹
worm (Caenorhabditis elegans)	Secernentea	T01C8.5³ got-1.2¹	aminotransferase³ got-1.2¹	53(a) (best of 3)³ 58.5(n)¹ 54.46(a)¹		X(16822325-16823912)³ 181726¹ NM_078308.5¹ NP_510709.1¹
baker's yeast (Saccharomyces cerevisiae)	Saccharomycetes	AAT2(YLR027C)⁴ AAT2¹	Cytosolic aspartate aminotransferase, involved in nitrogen more⁴ AAT2¹	51.8(n)¹ 48.12(a)¹		12(198085-196829)⁴ 850714^{1, 4} NP_013127.2^{1, 4}
thale cress (Arabidopsis thaliana)	eudicotyledons	ASP3¹	ASP3	55.61(n) 50.62(a)		831024 NM_121190.2 NP_196713.1
rice (Oryza sativa)	Liliopsida	Os01g0760600¹	Os01g0760600	55.94(n) 51.35(a)		4325621 NM_001050852.1 NP_001044317.1

ENSEMBL Gene Tree for GOT1 (if available)
TreeFam Gene Tree for GOT1 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68,Sets of similar genes according to GenesLikeMe)
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Paralogs for GOT1 gene

GOT2² GOT1L1²

3 SIMAP similar genes for GOT1 using alignment to 2 protein entries: AATC_HUMAN (see all proteins):

GIG18 GOT2 GOT1L1

Find genes that share paralogs with GOT1 About GenesLikeMe

1 Pseudogenes.org Pseudogene for GOT1
PGOHUM00000249433

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, ⁴UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Polymorphic Variants from UniProtKB/Swiss-Prot

AATC_HUMAN, P17174: Genetic variations in GOT1 are associated with low serum aspartate aminotransferase and define the

aspartate aminotransferase serum level quantitative trait locus 1 (ASTQTL1) [MIM:614419]

SNP ID	Valid	Clinical significance	Chr 10 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs199689809^1,2	C	--	101158427(+)	`AAAAA-/AGAATT`	1	--	int¹		0	--	--	--	--
rs5787336^1,2	C	--	101166940(+)	`CATTAGT/-GTGTG`	1	--	int¹		1		CSA	2
rs10668892^1,2	C	--	101167543(+)	`TATTTATTT/- TACTT`	1	--	int¹		1		NA	2
rs67270882^1,2	C	--	101177817(+)	`AAAAAT/AATATA`	1	--	int¹		1		NA	2
rs11394746^1,2	C	--	101178251(+)	`TTTTT-/T/TT CTTTT`	1	--	int¹ trp³		1		NA	2
rs149422963^1,2		--	101518023(+)	`GAATTA/GACTTT`	1	--	ds500¹		0	--	--	--	--
rs116276397^1,2	C,F	--	101518056(+)	`ATAGAC/TCTCTT`	1	--	ds500¹		1		WA	118
rs115451683^1,2	C,F	--	101518071(+)	`TAAGTA/GACATC`	1	--	ds500¹		1		WA	118
rs191576766^1,2		--	101518114(+)	`GGAGCA/GGCCCT`	1	--	ds500¹		0	--	--	--	--
rs12217584^1,2	F,A,H	--	101518204(+)	`CTGATG/TTTCTA`	1	--	ds500¹		4		NS EA	414

HapMap Linkage Disequilibrium report for GOT1 (101156627 - 101190530 bp)

Structural Variations
Database of Genomic Variants (DGV) variations for GOT1: --
Human Gene Mutation Database (HGMD): GOT1

Site Specific Mutation Identification with PCR Assays

SeqTarget long-range PCR primers for resequencing GOT1

DNA2.0 Custom Variant and Variant Library Synthesis for GOT1

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB, Sets of similar genes according to GenesLikeMe)
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OMIM gene information: 138180
OMIM disorders: 614419
5 diseases for GOT1:
About MalaCards

aspartate aminotransferase, serum level of, qtl1 exanthem gallbladder disease reye syndrome

acute pyelonephritis

Selected inferred disease relationships from the University of Copenhagen DISEASES database for GOT1 (see all 24):

Liver disease Fatty liver disease Hepatitis B Hepatitis C

Cholestasis Vascular disease Thrombocytopenia Hepatocellular carcinoma

Autoimmune hepatitis Cholangitis Diarrhea Myocardial infarction

Substance abuse Hepatitis A Leukopenia Diabetes mellitus

Bilirubin metabolic disorder Anemia Myopathy Exanthem

Find genes that share disorders with GOT1 About GenesLikeMe

Genetic Association Database (GAD): GOT1
Human Genome Epidemiology (HuGE) Navigator: GOT1 (3 documents)

Export disorders for GOT1 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for GOT1 gene, integrated from 10 sources (see all 82):
(articles sorted by number of sources associating them with GOT1)

Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection. (PubMed id 1974457)^{1, 2, 3} Bousquet-Lemercier B.... Barouki R. (Biochemistry 1990)
Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels. (PubMed id 21900944)^{1, 2} Shen H.... Gong D.W. (J. Hum. Genet. 2011)
Human variation in alcohol response is influenced by variation in neuronal signaling genes. (PubMed id 20201926)^{1, 4} Joslyn G....White R.L. (Alcohol. Clin. Exp. Res. 2010)
The DNA sequence and comparative analysis of human chromosome 10. (PubMed id 15164054)^{1, 2} Deloukas P.... Rogers J. (Nature 2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S.... Malek J. (Genome Res. 2004)
The amino acid sequence of cytosolic aspartate aminotransferase from human liver. (PubMed id 2241899)^{1, 2} Doyle J.M.... Doonan S. (Biochem. J. 1990)
Genetic homogeneity, high-resolution mapping, and mutation analysis of the urofacial (Ochoa) syndrome and exclusion of the glutamate oxaloacetate transaminase gene (GOT1) in the critical region as the disease gene. (PubMed id 10360399)^{1, 9} Wang C.Y....She J.X. (Am. J. Med. Genet. 1999)
Genome-wide association study of liver enzymes in korean children. (PubMed id 24124411)¹ Park T.J....Lee J.Y. (amp 2013)
Independent and supra-additive effects of alcohol consumption, cigarette smoking, and metabolic syndrome on the elevation of serum liver enzyme levels. (PubMed id 23667618)¹ Park E.Y....Shin H.R. (PLoS ONE 2013)
Elevated aspartate and alanine aminotransferase levels and natural death among patients with methamphetamine dependence. (PubMed id 22242166)¹ Kuo C.J....Chen W.J. (PLoS ONE 2012)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 2805	HGNC: 4432	AceView: GOT1	Ensembl:ENSG00000120053	euGenes: HUgn2805
ECgene: GOT1	Kegg: 2805	H-InvDB: GOT1

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for GOT1	Pharmacogenomics, SNPs, Pathways

Intellectual Property for GOT1 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for GOT1 gene:
Search GeneIP for patents involving GOT1

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, GeneTex, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from genOway)
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GOT1 Gene

Glutamic-Oxaloacetic Transaminase 1, Soluble

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GOT1 Gene

Glutamic-Oxaloacetic Transaminase 1, Soluble

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