Free for academic non-profit institutions. Other users need a Commercial license

Aliases for GNB2L1 Gene

Aliases for GNB2L1 Gene

  • Guanine Nucleotide Binding Protein (G Protein), Beta Polypeptide 2-Like 1 2 3
  • Guanine Nucleotide-Binding Protein Subunit Beta-Like Protein 12.3 3 4
  • Cell Proliferation-Inducing Gene 21 Protein 3 4
  • Receptor Of Activated Protein Kinase C 1 3 4
  • Human Lung Cancer Oncogene 7 Protein 3 4
  • Receptor For Activated C Kinase 1 2 3
  • HLC-7 3 4
  • RACK1 3 4
  • Protein Homologous To Chicken B Complex Protein, Guanine Nucleotide Binding 3
  • Guanine Nucleotide Binding Protein Beta Polypeptide 2-Like 1 3
  • Receptor For Activated C Kinase 4
  • Proliferation-Inducing Gene 21 3
  • Lung Cancer Oncogene 7 3
  • Gnb2-Rs1 3
  • H12.3 3
  • PIG21 3

External Ids for GNB2L1 Gene

Previous GeneCards Identifiers for GNB2L1 Gene

  • GC05M180713
  • GC05P181694
  • GC05M180773
  • GC05M180596
  • GC05M180663
  • GC05M175390

Summaries for GNB2L1 Gene

GeneCards Summary for GNB2L1 Gene

GNB2L1 (Guanine Nucleotide Binding Protein (G Protein), Beta Polypeptide 2-Like 1) is a Protein Coding gene. Diseases associated with GNB2L1 include hay-wells syndrome and wells syndrome. Among its related pathways are Signaling by GPCR and ERK Signaling. GO annotations related to this gene include poly(A) RNA binding and receptor binding. An important paralog of this gene is WDR31.

UniProtKB/Swiss-Prot for GNB2L1 Gene

  • Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed:19674157). Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells. Enhances phosphorylation of HIV-1 Nef by PKCs. Promotes migration of breast carcinoma cells by binding to and activating RHOA.

Gene Wiki entry for GNB2L1 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for GNB2L1 Gene

Genomics for GNB2L1 Gene

Regulatory Elements for GNB2L1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for GNB2L1 Gene

Chromosome:
5
Start:
181,236,909 bp from pter
End:
181,248,096 bp from pter
Size:
11,188 bases
Orientation:
Minus strand

Genomic View for GNB2L1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for GNB2L1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for GNB2L1 Gene

Proteins for GNB2L1 Gene

  • Protein details for GNB2L1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P63244-GBLP_HUMAN
    Recommended name:
    Guanine nucleotide-binding protein subunit beta-2-like 1
    Protein Accession:
    P63244
    Secondary Accessions:
    • B3KTJ0
    • D3DWS0
    • P25388
    • P99049
    • Q53HU2
    • Q5J8M6
    • Q5VLR4
    • Q6FH47

    Protein attributes for GNB2L1 Gene

    Size:
    317 amino acids
    Molecular mass:
    35077 Da
    Quaternary structure:
    • Interacts with CPNE3 (PubMed:20010870). May interact with ABCB4 (PubMed:19674157). Component of the small (40S) ribosomal subunit (By similarity). Interacts with the 80S ribosome. Exists as a monomer and also forms oligomers. Binds SLC9A3R1. Forms a ternary complex with TRIM63 and PRKCE. Interacts with HABP4, KRT1 and OTUB1. Interacts with SRC (via SH2 domain); the interaction is enhanced by tyrosine phosphorylation of GNB2L1/RACK1. Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and PRKCA. Interacts with AR. Interacts with IGF1R but not with INSR. Interacts with ADAM12. Interacts with CLEC1B (via N-terminal region) and with HIF1A; the interaction promotes their degradation. Interacts with RHOA; this enhances RHOA activation and promotes cell migration. Interacts with CHRM2; the interaction regulates CHRM2 internalization. Interacts with TRPM6 (via kinase domain). Interacts with PTK2/FAK1; required for PTK2/FAK1 phosphorylation and dephosphorylation. Interacts with FLT1. Interacts with TBXA2R isoform 2. Interacts with HRAS. Interacts with LARP4B. Interacts with PKD2L1. Interacts with Y.pseudotuberculosis yopK. Interacts with a number of viral proteins including Epstein-Barr virus BZLF1 and HIV-1 Nef; interaction with Nef increases Nef phosphorylation by PKC.
    SequenceCaution:
    • Sequence=AAO21313.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=AAR24619.1; Type=Frameshift; Positions=94; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for GNB2L1 Gene

neXtProt entry for GNB2L1 Gene

Proteomics data for GNB2L1 Gene at MOPED

Post-translational modifications for GNB2L1 Gene

  • Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC.
  • Ubiquitination at Lys 12, Lys 38, Lys 44, Lys 106, Lys 130, Lys 139, Lys 172, Lys 175, Lys 183, Lys 185, Lys 212, Lys 257, Lys 264, and Lys 271
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for GNB2L1 (RACK1)

No data available for DME Specific Peptides for GNB2L1 Gene

Domains & Families for GNB2L1 Gene

Suggested Antigen Peptide Sequences for GNB2L1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P63244

UniProtKB/Swiss-Prot:

GBLP_HUMAN :
  • Belongs to the WD repeat G protein beta family.
  • Contains 7 WD repeats.
Family:
  • Belongs to the WD repeat G protein beta family.
Similarity:
  • Contains 7 WD repeats.
genes like me logo Genes that share domains with GNB2L1: view

No data available for Gene Families for GNB2L1 Gene

Function for GNB2L1 Gene

Molecular function for GNB2L1 Gene

GENATLAS Biochemistry:
guanine nucleotide binding protein (G protein),beta 2 like 1,involved in membrane aanchorage of protein kinase C,homolog of a beta subunit implicated in membrane anchorage of the beta adrenergic receptor
UniProtKB/Swiss-Prot Function:
Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed:19674157). Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells. Enhances phosphorylation of HIV-1 Nef by PKCs. Promotes migration of breast carcinoma cells by binding to and activating RHOA.

Gene Ontology (GO) - Molecular Function for GNB2L1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005080 protein kinase C binding IDA 11279199
GO:0005102 receptor binding NAS 12809483
GO:0005515 protein binding IPI 10849009
GO:0008200 ion channel inhibitor activity ISS --
GO:0008656 cysteine-type endopeptidase activator activity involved in apoptotic process IMP 19767770
genes like me logo Genes that share ontologies with GNB2L1: view
genes like me logo Genes that share phenotypes with GNB2L1: view

Animal Model Products

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for GNB2L1

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targets and HOMER Transcription for GNB2L1 Gene

Localization for GNB2L1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for GNB2L1 Gene

Cell membrane; Peripheral membrane protein. Cytoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Nucleus. Perikaryon. Cell projection, dendrite. Cell projection, phagocytic cup. Note=Recruited to the plasma membrane through interaction with KRT1 which binds to membrane-bound ITGB1. Also associated with the membrane in oncogene-transformed cells. PKC activation induces translocation from the perinuclear region to the cell periphery. In the brain, detected mainly in cell bodies and dendrites with little expression in axonal fibers or nuclei. Localized to phagocytic cups following infection by Y.pestis.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for GNB2L1 Gene COMPARTMENTS Subcellular localization image for GNB2L1 gene
Compartment Confidence
extracellular 5
nucleus 5
plasma membrane 5
cytosol 4
mitochondrion 4
cytoskeleton 3

Gene Ontology (GO) - Cellular Components for GNB2L1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001891 phagocytic cup IDA 21347310
GO:0005634 nucleus IDA 10849009
GO:0005737 cytoplasm IDA 10849009
GO:0005739 mitochondrion IMP 19767770
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with GNB2L1: view

Pathways & Interactions for GNB2L1 Gene

genes like me logo Genes that share pathways with GNB2L1: view

SIGNOR curated interactions for GNB2L1 Gene

Activates:
Is activated by:

Gene Ontology (GO) - Biological Process for GNB2L1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001649 osteoblast differentiation IEA --
GO:0001934 positive regulation of protein phosphorylation IDA 11312657
GO:0006919 activation of cysteine-type endopeptidase activity involved in apoptotic process IMP 19767770
GO:0007049 cell cycle IEA --
GO:0007166 cell surface receptor signaling pathway TAS --
genes like me logo Genes that share ontologies with GNB2L1: view

Drugs & Compounds for GNB2L1 Gene

(9) Drugs for GNB2L1 Gene - From: NovoSeek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Ethanol Approved Pharma 1708
Phosphatidylserine Approved Nutra 0
farnesyl diphosphate Experimental Pharma Antagonist 0
NMDA Pharma Full agonist, Agonist 0
alanine Nutra 97

(9) Additional Compounds for GNB2L1 Gene - From: NovoSeek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
17-(allylamino)-17-demethoxygeldanamycin
adenylate
geranylgeranyl pyrophosphate
glutamate
inositol 1,4,5 trisphosphate
genes like me logo Genes that share compounds with GNB2L1: view

Transcripts for GNB2L1 Gene

Unigene Clusters for GNB2L1 Gene

Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for GNB2L1

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for GNB2L1 Gene

No ASD Table

Relevant External Links for GNB2L1 Gene

GeneLoc Exon Structure for
GNB2L1
ECgene alternative splicing isoforms for
GNB2L1

Expression for GNB2L1 Gene

mRNA expression in normal human tissues for GNB2L1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for GNB2L1 Gene



SOURCE GeneReport for Unigene cluster for GNB2L1 Gene Hs.5662

mRNA Expression by UniProt/SwissProt for GNB2L1 Gene

P63244-GBLP_HUMAN
Tissue specificity: In the liver, expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. Up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue.
genes like me logo Genes that share expression patterns with GNB2L1: view

Protein tissue co-expression partners for GNB2L1 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues and Protein differential expression in normal tissues for GNB2L1 Gene

Orthologs for GNB2L1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for GNB2L1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia GNB2L1 35
  • 92.22 (n)
  • 100 (a)
GNB2L1 36
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia GNB2L1 35
  • 90.12 (n)
  • 100 (a)
GNB2L1 36
  • 100 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Gnb2l1 35
  • 90.85 (n)
  • 100 (a)
Gnb2l1 16
Gnb2l1 36
  • 100 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia GNB2L1 35
  • 99.47 (n)
  • 100 (a)
GNB2L1 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Gnb2l1 35
  • 90.96 (n)
  • 100 (a)
oppossum
(Monodelphis domestica)
Mammalia GNB2L1 36
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia GNB2L1 36
  • 100 (a)
OneToOne
chicken
(Gallus gallus)
Aves GNB2L1 35
  • 85.38 (n)
  • 100 (a)
GNB2L1 36
  • 100 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia GNB2L1 36
  • 100 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia gnb2l1 35
  • 80.97 (n)
  • 96.21 (a)
Str.6701 35
African clawed frog
(Xenopus laevis)
Amphibia gnb2l1-prov 35
zebrafish
(Danio rerio)
Actinopterygii gnb2l1 35
  • 83.81 (n)
  • 95.58 (a)
gnb2l1 36
  • 96 (a)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.1296 35
fruit fly
(Drosophila melanogaster)
Insecta Rack1 37
  • 77 (a)
Rack1 35
  • 72.51 (n)
  • 77.39 (a)
Rack1 36
  • 76 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP010173 35
  • 70.09 (n)
  • 74.36 (a)
worm
(Caenorhabditis elegans)
Secernentea K04D7.1 37
  • 69 (a)
rack-1 35
  • 68.05 (n)
  • 73.57 (a)
rack-1 36
  • 68 (a)
OneToOne
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AFR199C 35
  • 59.07 (n)
  • 57.33 (a)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0E12277g 35
  • 55.74 (n)
  • 53.44 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ASC1 35
  • 54.83 (n)
  • 55.05 (a)
ASC1 36
  • 51 (a)
OneToMany
ASC1 38
thale cress
(Arabidopsis thaliana)
eudicotyledons RACK1C_AT 35
  • 61.97 (n)
  • 68.59 (a)
rice
(Oryza sativa)
Liliopsida Os05g0552300 35
  • 64.83 (n)
  • 67.31 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU05810 35
  • 68.7 (n)
  • 71.47 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes cpc2 35
  • 63.47 (n)
  • 64.22 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.4423 36
  • 73 (a)
OneToOne
Species with no ortholog for GNB2L1:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for GNB2L1 Gene

ENSEMBL:
Gene Tree for GNB2L1 (if available)
TreeFam:
Gene Tree for GNB2L1 (if available)

Paralogs for GNB2L1 Gene

Paralogs for GNB2L1 Gene

Pseudogenes.org Pseudogenes for GNB2L1 Gene

genes like me logo Genes that share paralogs with GNB2L1: view

Variants for GNB2L1 Gene

Structural Variations from Database of Genomic Variants (DGV) for GNB2L1 Gene

Variant ID Type Subtype PubMed ID
nsv428133 CNV Gain 18775914
nsv883305 CNV Gain 21882294
nsv830567 CNV Loss 17160897
dgv6408n71 CNV Loss 21882294
nsv517697 CNV Loss 19592680
dgv6409n71 CNV Loss 21882294
nsv462591 CNV Loss 19166990

Variation tolerance for GNB2L1 Gene

Residual Variation Intolerance Score: 28.85% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.48; 28.89% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for GNB2L1 Gene

HapMap Linkage Disequilibrium report
GNB2L1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations from dbSNP and Humsavar for GNB2L1 Gene

Disorders for GNB2L1 Gene

MalaCards: The human disease database

(3) MalaCards diseases for GNB2L1 Gene - From: GeneCards

Disorder Aliases PubMed IDs
hay-wells syndrome
  • aec syndrome
wells syndrome
  • bullous cellulitis with eosinophilia
hiv-1
  • aids, slow progression to
- elite association

Relevant External Links for GNB2L1

Genetic Association Database (GAD)
GNB2L1
Human Genome Epidemiology (HuGE) Navigator
GNB2L1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
GNB2L1
genes like me logo Genes that share disorders with GNB2L1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for GNB2L1 Gene

Publications for GNB2L1 Gene

  1. Cloning of an intracellular receptor for protein kinase C: a homolog of the beta subunit of G proteins. (PMID: 8302854) Ron D. … Mochly-Rosen D. (Proc. Natl. Acad. Sci. U.S.A. 1994) 2 23 67
  2. Depletion of WRN protein causes RACK1 to activate several protein kinase C isoforms. (PMID: 19966859) Massip L. … Lebel M. (Oncogene 2010) 23 67
  3. Expression of RACK1 is a novel biomarker in pulmonary adenocarcinomas. (PMID: 19892429) Nagashio R. … Okayasu I. (Lung Cancer 2010) 23 67
  4. Functional mapping of the promoter region of the GNB2L1 human gene coding for RACK1 scaffold protein. (PMID: 19000751) Del Vecchio I. … Racchi M. (Gene 2009) 23 67
  5. Phosphorylation of RACK1 on tyrosine 52 by c-Abl is required for insulin-like growth factor I-mediated regulation of focal adhesion kinase. (PMID: 19423701) Kiely P.A. … O'Connor R. (J. Biol. Chem. 2009) 23 67

Products for GNB2L1 Gene

Sources for GNB2L1 Gene

Back to Top

Content