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Aliases for GNAT3 Gene

Aliases for GNAT3 Gene

  • Guanine Nucleotide Binding Protein, Alpha Transducing 3 2 3
  • Gustducin Alpha-3 Chain 3 4
  • Guanine Nucleotide-Binding Protein G(T) Subunit Alpha-3 3
  • GDCA 3

External Ids for GNAT3 Gene

Previous GeneCards Identifiers for GNAT3 Gene

  • GC07M079700
  • GC07M079732
  • GC07M079925
  • GC07M080087
  • GC07M074691

Summaries for GNAT3 Gene

GeneCards Summary for GNAT3 Gene

GNAT3 (Guanine Nucleotide Binding Protein, Alpha Transducing 3) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Signaling by GPCR. GO annotations related to this gene include GTP binding and GTPase activity. An important paralog of this gene is GNAQ.

UniProtKB/Swiss-Prot for GNAT3 Gene

  • Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.

Tocris Summary for GNAT3 Gene

  • Heterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G protein contains an alpha-, beta- and gamma-subunit and is bound to GDP in the 'off' state. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins; Galphas and Galphai bind directly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease in cAMP levels respectively.

Gene Wiki entry for GNAT3 Gene

No data available for Entrez Gene Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for GNAT3 Gene

Genomics for GNAT3 Gene

Regulatory Elements for GNAT3 Gene

Genomic Location for GNAT3 Gene

Start:
80,458,671 bp from pter
End:
80,512,020 bp from pter
Size:
53,350 bases
Orientation:
Minus strand

Genomic View for GNAT3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for GNAT3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for GNAT3 Gene

Proteins for GNAT3 Gene

  • Protein details for GNAT3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    A8MTJ3-GNAT3_HUMAN
    Recommended name:
    Guanine nucleotide-binding protein G(t) subunit alpha-3
    Protein Accession:
    A8MTJ3
    Secondary Accessions:
    • A4D1B2
    • A4D1B3
    • B9EJG5

    Protein attributes for GNAT3 Gene

    Size:
    354 amino acids
    Molecular mass:
    40357 Da
    Quaternary structure:
    • G proteins are composed of 3 units; alpha, beta and gamma, respectively GNAT3, GNB1 and GNG13 for Gustducin heterotrimer for bitter taste transduction. The alpha chain contains the guanine nucleotide binding site. Gustducin heterotrimer may also be composed of GNAT3, GNB3 and GNG13
    SequenceCaution:
    • Sequence=EAL24192.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=EAL24193.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

neXtProt entry for GNAT3 Gene

Proteomics data for GNAT3 Gene at MOPED

Post-translational modifications for GNAT3 Gene

  • Potential N-myristoylation may anchor alpha-subunit to the inner surface of plasma membrane.
  • Ubiquitination at Lys46
  • Modification sites at PhosphoSitePlus

Other Protein References for GNAT3 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for GNAT3 Gene

Domains for GNAT3 Gene

Protein Domains for GNAT3 Gene

Suggested Antigen Peptide Sequences for GNAT3 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

A8MTJ3

UniProtKB/Swiss-Prot:

GNAT3_HUMAN :
  • A8MTJ3
Family:
  • Belongs to the G-alpha family. G(i/o/t/z) subfamily.
genes like me logo Genes that share domains with GNAT3: view

No data available for Gene Families for GNAT3 Gene

Function for GNAT3 Gene

Molecular function for GNAT3 Gene

UniProtKB/Swiss-Prot Function: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.

Gene Ontology (GO) - Molecular Function for GNAT3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001664 G-protein coupled receptor binding IBA --
GO:0003924 GTPase activity IBA --
GO:0004871 signal transducer activity --
GO:0005525 GTP binding IEA --
GO:0008020 G-protein coupled photoreceptor activity IBA --
genes like me logo Genes that share ontologies with GNAT3: view

Phenotypes for GNAT3 Gene

genes like me logo Genes that share phenotypes with GNAT3: view

Animal Models for GNAT3 Gene

MGI Knock Outs for GNAT3:

Animal Model Products

CRISPR Products

No data available for Enzyme Numbers (IUBMB) , miRNA , Transcription Factor Targeting and HOMER Transcription for GNAT3 Gene

Localization for GNAT3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for GNAT3 Gene

Cytoplasm. Note=Dual ditribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for GNAT3 Gene COMPARTMENTS Subcellular localization image for GNAT3 gene
Compartment Confidence
cytosol 3
plasma membrane 3
mitochondrion 2
cytoskeleton 1
extracellular 1
golgi apparatus 1
nucleus 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for GNAT3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001669 acrosomal vesicle IEA --
GO:0001750 photoreceptor outer segment IBA --
GO:0001917 photoreceptor inner segment IBA --
GO:0005737 cytoplasm --
GO:0005834 heterotrimeric G-protein complex IBA --
genes like me logo Genes that share ontologies with GNAT3: view

Pathways for GNAT3 Gene

genes like me logo Genes that share pathways with GNAT3: view

Pathways by source for GNAT3 Gene

Gene Ontology (GO) - Biological Process for GNAT3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001580 detection of chemical stimulus involved in sensory perception of bitter taste IBA --
GO:0006184 obsolete GTP catabolic process --
GO:0007165 signal transduction --
GO:0007186 G-protein coupled receptor signaling pathway --
GO:0007188 adenylate cyclase-modulating G-protein coupled receptor signaling pathway IBA --
genes like me logo Genes that share ontologies with GNAT3: view

Compounds for GNAT3 Gene

(5) Tocris Compounds for GNAT3 Gene

Compound Action Cas Number
8-Bromo-cGMP, sodium salt cGMP analog; activates PKG [51116-01-9]
CCG 50014 Potent and selective inhibitor of regulator of G-protein signaling 4 (RGS4) protein [883050-24-6]
Gallein Inhibitor of betagamma signaling [2103-64-2]
Pertussis Toxin Catalyzes ADP-ribosylation of Gi, Go and Gt [70323-44-3]
SCH 202676 hydrobromide Inhibitor of ligand binding to G-protein-coupled receptors [265980-25-4]

(2) HMDB Compounds for GNAT3 Gene

Compound Synonyms Cas Number PubMed IDs
Calcium
  • Ca
7440-70-2
Cyclic AMP
  • Cyclic AMP
60-92-4
genes like me logo Genes that share compounds with GNAT3: view

Transcripts for GNAT3 Gene

mRNA/cDNA for GNAT3 Gene

(2) Additional mRNA sequences :
(4) REFSEQ mRNAs :
(1) Selected AceView cDNA sequences:
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for GNAT3 Gene

Guanine nucleotide binding protein, alpha transducing 3:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for GNAT3

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for GNAT3 Gene

No ASD Table

Relevant External Links for GNAT3 Gene

GeneLoc Exon Structure for
GNAT3
ECgene alternative splicing isoforms for
GNAT3

Expression for GNAT3 Gene

mRNA expression in normal human tissues for GNAT3 Gene

mRNA differential expression in normal tissues according to GTEx for GNAT3 Gene

This gene is overexpressed in Small Intestine - Terminal Ileum (13.7), Adipose - Visceral (Omentum) (12.5), Testis (10.6), and Thyroid (6.1).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for GNAT3 Gene

SOURCE GeneReport for Unigene cluster for GNAT3 Gene Hs.335049

mRNA Expression by UniProt/SwissProt for GNAT3 Gene

A8MTJ3-GNAT3_HUMAN
Tissue specificity: Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and foliate papillae of the tongue at protein level. Expressed in enteroendocrine L cells of the gut. Detected also in spermatozoa.
genes like me logo Genes that share expressions with GNAT3: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery for GNAT3 Gene

Orthologs for GNAT3 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for GNAT3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia GNAT3 35
  • 99.72 (n)
  • 100 (a)
GNAT3 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia GNAT3 35
  • 93.03 (n)
  • 96.33 (a)
GNAT3 36
  • 89 (a)
OneToOne
dog
(Canis familiaris)
Mammalia GNAT3 35
  • 92.18 (n)
  • 96.89 (a)
GNAT3 36
  • 97 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Gnat3 35
  • 88.04 (n)
  • 95.48 (a)
Gnat3 16
Gnat3 36
  • 95 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia GNAT3 36
  • 94 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia GNAT3 36
  • 88 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Gnat3 35
  • 88.42 (n)
  • 95.76 (a)
chicken
(Gallus gallus)
Aves GNAT3 35
  • 78.63 (n)
  • 81.92 (a)
GNAT3 36
  • 82 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia GNAT3 36
  • 85 (a)
OneToOne
zebrafish
(Danio rerio)
Actinopterygii gnao1b 36
  • 60 (a)
ManyToMany
fruit fly
(Drosophila melanogaster)
Insecta G-oalpha47A 36
  • 58 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea goa-1 36
  • 56 (a)
OneToMany
thale cress
(Arabidopsis thaliana)
eudicotyledons GP ALPHA 1 35
  • 51.34 (n)
  • 39.37 (a)
rice
(Oryza sativa)
Liliopsida Os05g0333200 35
  • 52.96 (n)
  • 41.42 (a)
Species with no ortholog for GNAT3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for GNAT3 Gene

ENSEMBL:
Gene Tree for GNAT3 (if available)
TreeFam:
Gene Tree for GNAT3 (if available)

Paralogs for GNAT3 Gene

Paralogs for GNAT3 Gene

Selected SIMAP similar genes for GNAT3 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with GNAT3: view

Variants for GNAT3 Gene

Sequence variations from dbSNP and Humsavar for GNAT3 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type MAF
rs799927 -- 80,465,291(+) AGATC(A/G)TGACT intron-variant
rs799928 -- 80,466,773(+) ACTCA(A/G)TGGCT intron-variant
rs799929 -- 80,468,260(+) ATCAC(A/G)TGAGA intron-variant
rs799930 -- 80,469,171(+) AAAAC(C/T)GTAGT intron-variant
rs799931 -- 80,470,038(+) GTTTT(C/T)AGAAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for GNAT3 Gene

Variant ID Type Subtype PubMed ID
nsv888512 CNV Loss 21882294
nsv831042 CNV Gain 17160897
nsv888523 CNV Loss 21882294
nsv888524 CNV Gain 21882294
nsv831043 CNV Loss 17160897
nsv464604 CNV Loss 19166990
esv2734733 CNV Deletion 23290073
esv2734734 CNV Deletion 23290073
esv2734735 CNV Deletion 23290073
esv2734736 CNV Deletion 23290073
esv3363 CNV Deletion 18987735
nsv365325 CNV Loss 16902084

Relevant External Links for GNAT3 Gene

HapMap Linkage Disequilibrium report
GNAT3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for GNAT3 Gene

Disorders for GNAT3 Gene

Relevant External Links for GNAT3

Genetic Association Database (GAD)
GNAT3
Human Genome Epidemiology (HuGE) Navigator
GNAT3
genes like me logo Genes that share disorders with GNAT3: view

No data available for UniProtKB/Swiss-Prot for GNAT3 Gene

Publications for GNAT3 Gene

  1. Colocalization of the alpha-subunit of gustducin with PYY and GLP-1 in L cells of human colon. (PMID: 16728727) Rozengurt N. … Rozengurt E. (Am. J. Physiol. 2006) 3 4 23
  2. Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1. (PMID: 17724330) Jang H.-J. … Egan J.M. (Proc. Natl. Acad. Sci. U.S.A. 2007) 3 4 23
  3. Human taste cells express the G protein alpha-gustducin and neuron- specific enolase. (PMID: 8015379) Takami S. … Getchell M.L. (Brain Res. Mol. Brain Res. 1994) 3 4
  4. Ggamma13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium. (PMID: 10570481) Huang L. … Margolskee R.F. (Nat. Neurosci. 1999) 3 23
  5. Human receptors for sweet and umami taste. (PMID: 11917125) Li X. … Adler E. (Proc. Natl. Acad. Sci. U.S.A. 2002) 3 4

Products for GNAT3 Gene

Sources for GNAT3 Gene

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