GNAI2 Gene
protein-coding GIFtS : 70 GCID: GC03 P050263
guanine nucleotide binding protein (G protein), alpha inhibiting... GNAI2B )
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Aliasesfor GNAI2 gene
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc ,
7 Ensembl ,
8 DME ,
9 miRBase ,
and/or 10 fRNAdb )About This Section Aliases Guanine Nucleotide Binding Protein (G Protein), Alpha Inhibiting ActivityPolypeptide 2 1 2 H_LUCA16.12 GNAI2B1 2 3 5 GTP-Binding Regulatory Protein Gi Alpha-2 Chain2 GIP1 2 5 Guanine Nucleotide-Binding Protein G(I) Subunit Alpha-22 Adenylate Cyclase-Inhibiting G Alpha Protein2 3 Guanine Nucleotide-Binding Protein G(I), Alpha-2 Subunit2 H_LUCA15.12
Export aliases for GNAI2 gene to outside databases Previous GC identifers: GC03M049690 GC03M049860 GC03P050118 GC03P050223 GC03P050232 GC03P050239
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Summariesfor GNAI2 gene (According to Entrez Gene ,
Tocris Bioscience ,
Wikipedia's
Gene Wiki ,
PharmGKB ,UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
About This Section Entrez Gene summary for GNAI2 : The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only two are known so far. (provided by RefSeq, Oct 2009) UniProtKB/Swiss-Prot: GNAI2_HUMAN, P04899 Function : Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in varioustransmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division Function : Isoform sGi2: Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as anintracellular pool summary
for GNAI2 : Heterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G proteincontains an alpha-, beta- and gamma-subunit and is bound to GDP in the 'off' state. Ligand-receptor bindingresults in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation ofsecond messenger signalling cascades. There are several types of Galpha proteins; Galphas and Galphai binddirectly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease incAMP levels respectively. Gene Wiki entry for GNAI2
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Genomic Viewsfor GNAI2 gene
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 37) ,miRBase ,UCSC (hg19) and
Ensembl (release 69) ,
Regulatory elements and Epigenetics data according to
QIAGEN ,
SABiosciences , and/or
SwitchGear Genomics )About This Section RefSeq DNA sequence: NC_000003.11 NC_018914.1 NT_022517.18 Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the GNAI2 gene promoter:SRF CHOP-10 GR Sp1 SRF (504 AA) GR-alpha C/EBPalpha Other transcription factors Search SABiosciences Chromatin IP Primers for GNAI2 Epigenetics: QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human mouse rat
Genomic Location: Genomic View : UCSC Golden Path with GeneCards custom track Entrez Gene cytogenetic band: 3p21.31 Ensembl cytogenetic band: 3p21.31 HGNC cytogenetic band: 3p21.31 GNAI2 Gene in genomic location: bands according to Ensembl, locations according to
Entrez Gene Ensembl GeneLoc information about chromosome 3 GeneLoc Exon Structure
GeneLoc location for GC03P050263: view genomic region
(about GC identifiers )
Start: 50,263,724 bp from pter End:
50,296,787 bp from pter
Size: 33,064 bases Orientation:
plus strand
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Proteinsfor GNAI2 gene
(According to
1 UniProtKB ,
HORDE ,
neXtProt ,
Ensembl ,
and/or Reactome ,
Modification sites according to 2 PhosphoSitePlus ,
Specific Peptides from DME ,
Protein expression images according to data from SPIRE MOPED and PaxDb ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from
EMD Millipore ,
R&D Systems ,
GenScript ,
Enzo Life Sciences ,
OriGene ,
Novus Biologicals ,
Sino Biological ,
ProSpec , and/or
Uscn ,
EMD Millipore ,
R&D Systems ,
OriGene ,
GenScript ,
Cell Signaling Technology ,
Enzo Life Sciences , and/or
Uscn ,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene ,
Antibodies by
EMD Millipore ,
R&D Systems ,
GenScript ,
Cell Signaling Technology ,
OriGene ,
Novus Biologicals ,
Thermo Fisher Scientific ,
Abcam , and/or
Uscn )
About This Section UniProtKB/Swiss-Prot: GNAI2_HUMAN, P04899 See
protein sequence )Recommended Name: Size : 355 amino acids; 40451 DaSubunit : G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotidebinding site. Interacts with GPSM1. Interacts with RGS12 and RGS14 (By similarity). Interacts with UNC5B
Subcellular location : Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Cell membrane. Note=Localizes in the centrosomesof interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody
Secondary accessions : B4DYA0 Q6B6N3 Q8IZ71Alternative splicing : 4 isoforms : P04899-1 P04899-2 P04899-3 P04899-4 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for GNAI2: NX_P04899 Post-translational modifications:
View modification sites using PhosphoSitePlus 2 View neXtProt modification sites for NX_P04899 GNAI2 Protein expression data from MOPED and PaxDb : About this image REFSEQ proteins (2 alternative transcripts): NP_001159897.1 NP_002061.1 ENSEMBL proteins: ENSP00000406871 ENSP00000406065 ENSP00000312999 ENSP00000394321 ENSP00000395736 ENSP00000406369 ENSP00000266027 ENSP00000444360 Reactome Protein details: P04899 Human Recombinant Protein Products: Gene Ontology (GO): 5/10 cellular component terms (GO ID links to tree view) (see all 10 ): About this table
About GeneDecksing GNAI2 Antibody Products: Assay Products for GNAI2:
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Protein
Domains /for GNAI2 gene (According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
About This Section About GeneDecksing 3 InterPro domains/families :
Graphical View of Domain Structure for InterPro Entry P04899 ProtoNet protein and cluster: P04899
2 Blocks protein families : IPB001019 IPB001408 UniProtKB/Swiss-Prot: GNAI2_HUMAN, P04899 Similarity : Belongs to the G-alpha family. G(i/o/t/z) subfamily
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Functionfor GNAI2 gene
(According to 1 UniProtKB ,
Genatlas ,
LifeMap Discovery™ ,
IUBMB , and/or
2 DME ,
Human phenotypes from GenomeRNAi ,
Animal models from MGI Mar 06 2013,
SABiosciences ,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene ,
RNAi from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
microRNA from QIAGEN ,
Gene Editing from DNA2.0 ,
Clones from EMD Millipore ,
OriGene ,
SwitchGear Genomics ,
GenScript ,
Sino Biological ,
DNA2.0 ,
and Vector BioLabs ,
Cell Lines from GenScript ,
LifeMap BioReagents ,
In Situ Hybridization Assays from Advanced Cell Diagnostics ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene .)
About This Section Function Summary: UniProtKB/Swiss-Prot: GNAI2_HUMAN, P04899 Function : Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in varioustransmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division Function : Isoform sGi2: Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as anintracellular pool
Genatlas biochemistry entry for GNAI2 guanine nucleotide binding protein (G protein),alpha inhibiting activity,polypeptide 2 Clone Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human mouse rat see all 4 )human mouse rat see all 3 )OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector (see all 2 ): GNAI2 (NM_002070 ) Browse Sino Biological Human cDNA Clones DNA2.0 Custom Codon Optimized Gene
Synthesis Service for GNAI2 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat GNAI2
In Situ AssayAdvanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GNAI2
Gene Ontology (GO): 5/8 molecular function terms (GO ID links to tree view) (see all 8 ): About this table
About GeneDecksing Animal Models: Gnai2 tm1Uru Gnai2 tm1Lbi Gnai2 tm1Rmm 13 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Gnai2) :About GeneDecksing
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Pathways & Interactionsfor GNAI2 gene
(Pathways according to
EMD Millipore ,
R&D Systems ,
Cell Signaling Technology ,
KEGG ,
PharmGKB ,
BioSystems ,
Reactome ,
Tocris Bioscience ,
GeneGo (Thomson Reuters) ,
QIAGEN ,
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Interaction Networks according to
SABiosciences ,
and/or STRING ,
Interactions according to 1 UniProtKB ,
2 MINT ,
3 I2D , and/or
4 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene) .
About This Section Unified GeneCards pathways - 5/78 super-pathways (see all 78 ) About this table See pathways by source Super-pathway contained gene-specific pathways 1 Development Angiotensin activation of ERK 2 Development_Dopamine D2 receptor transactivation of EGFR 3 Opioid Signalling 4 G Protein Signaling Pathways 5 G-Beta Gamma Signaling
Pathway sources See GeneCards unified pathways Show all pathways 5/23 (see all 23 )5/57 (see all 57 )5/25 (see all 25 )5/13 (see all 13 ) 5/24 (see all 24 )3 GNAI2 5/14 (Kegg details for GNAI2) (see all 14 ):About GeneDecksing Interactions: SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for GNAI2 STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)5/351 Interacting proteins for GNAI2 (P04899 1 , 2 , 3 ENSP00000312999 4 via UniProtKB, MINT, STRING , and/or I2D (see all 351 )About this table Gene Ontology (GO): 5/21 biological process terms (GO ID links to tree view) (see all 21 ): About this table
About GeneDecksing
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Drugs & Compoundsfor GNAI2 gene (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
EMD Millipore , Tocris Bioscience
HMDB ,
BitterDB , and/or
Novoseek , and Drugs according to
DrugBank ,
Enzo Life Sciences , and/or
PharmGKB , with drugs/clinical trials/news
search links to CenterWatch )
About This Section Compounds for GNAI2 available from Tocris Bioscience About this table Compound Action
CAS
# Mastoparan X G protein activator peptide [72093-22-2] Mastoparan Activates Gi and Go [72093-21-1] Pertussis Toxin Catalyzes ADP-ribosylation of Gi, Go and Gt [70323-44-3] Melittin Inhibits Gs and stimulates Gi activity [20449-79-0] NF 023 Selective, competitive P2X antagonist [104869-31-0]
3 HMDB Compounds for GNAI2 About this table GNAI2
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Transcriptsfor GNAI2 gene (Secondary structures according to
fRNAdb ,Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank , Entrez Gene ,
DOTS (version 10), and/or
AceView ,
transcript ids from Ensembl
with links to UCSC , GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,EMD Millipore ,
OriGene ,
QIAGEN ,
shRNA from
OriGene ,
microRNA from QIAGEN ,
OriGene ,
SwitchGear Genomics ,
GenScript ,
DNA2.0 ,
Vector BioLabs ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN
)About This Section REFSEQ mRNAs for GNAI2 gene (2 alternative transcripts): NM_001166425.1 NM_002070.2 Unigene Cluster for GNAI2:
Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 Hs.77269 [show with all ESTs ] Unigene Representative Sequence: AK126708 13 Ensembl transcripts including schematic representations, and UCSC links where relevant : ENST00000422163 (uc003cyo.1 uc003cyp.1 ) ENST00000446079 ENST00000491100 ENST00000480090 ENST00000313601 (uc003cyq.1 uc011bdn.2 ) ENST00000441156 (uc010hlg.1 )ENST00000440628 ENST00000451956 ENST00000490122 (uc003cyr.1 ) ENST00000468422 ENST00000492383 ENST00000266027 ENST00000536647 Clone OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human mouse rat see all 4 )human mouse rat see all 3 )OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector (see all 2 ): GNAI2 (NM_002070 ) DNA2.0 Custom Codon Optimized Gene
Synthesis Service for GNAI2 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat GNAI2
Additional cDNA sequence: AF493906.1 AK055574.1 AK096299.1 AK096595.1 AK126708.1 AK131062.1 AK302330.1 AK304473.1 AY555145.1 AY677118.1 BC012138.1 BC014627.2 BC016995.1 CR456783.1 J03004.1 X04828.1
24/42 DOTS entries (see all 42 ): DT.100680604 DT.97768608
DT.75112974 DT.99968212 DT.91784324 DT.97860778 DT.100680607 DT.100057602 DT.95357437 DT.97800888 DT.100059846 DT.100862119 DT.91784320 DT.100059845 DT.120873963 DT.100680601 DT.95214674 DT.100648169 DT.100680617 DT.120873971 DT.95185336 DT.120874020 DT.100042317 DT.100859294 24/1325 AceView cDNA sequences (see all 1325 ):
BQ680845 CR606737 AF493906 BU730850 BI032154 BM663450 BE741703 BQ895963 CD515989 AI066694 BQ009020 BU689061 BM708555 BU153196 AK096595 BQ893578 BQ927770 BE302918 AI654458 BQ899266 BU158574 CA393116 BG165758 BQ676408 GeneLoc Exon Structure 5/12 Alternative Splicing Database (ASD) splice patterns (SP) for GNAI2 (see all 12 ) About this scheme ExUns: 1a · 1b ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14a · 14b ^ 15a · 15b · 15c · 15d · 15e · SP1 : - - - - - - - - - - SP2 : - - - - - - SP3 : - - - - - - - - - - - SP4 : - - - - - - - - - SP5 : - - - - - - -
ExUns: 15f SP1 : SP2 : SP3 : SP4 : SP5 :
ECgene alternative splicing isoforms for GNAI2
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Expression for GNAI2 gene
(RNA expression data according to
H-InvDB ,
NONCODE ,
miRBase , and
RNAdb ,
Expression images according to data from
BioGPS ,
Illumina Human BodyMap , and
CGAP
SAGE ,
Sets of similar genes according to GeneDecks ,
in vivo and in vitro expression data from LifeMap Discovery™ ,
plus additional links to
Genevestigator , and/or
SOURCE , and/or
BioGPS , and/or
UniProtKB ,SABiosciences ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN ,
In Situ Hybridization Assays from Advanced Cell Diagnostics )
About This Section GNAI2 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this image BioGPS CGAP TAG: TTTTATGGAA
About this image GNAI2 expression in embryonic tissues and stem cells About this table See GNAI2 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for GNAI2 SOURCE GeneReport for Unigene cluster: Hs.77269 SABiosciences Expression via Pathway-Focused PCR Arrays including GNAI2 : Primer OriGene genome-wide validated SYBR primer pairs in human mouse rat Browse OriGene validated miRNA SYBR primer pairs SABiosciences RT2 qPCR Primer Assay in human mouse rat QIAGEN QuantiTect SYBR Green Assays in human mouse rat QIAGEN QuantiFast Probe-based Assays in human mouse rat In Situ Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GNAI2
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Orthologsfor GNAI2 gene
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
,
5 MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase ,
and/or
6 Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam )
About This Section Orthologs for GNAI2 gene from 9/31 species (see all 31 ) About this table
ENSEMBL Gene Tree for GNAI2 (if available)TreeFam Gene Tree for GNAI2 (if available)
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Paralogsfor GNAI2 gene (Paralogs according to
1 HomoloGene ,2 Ensembl , and 3 SIMAP , Pseudogenes according to 4 Pseudogene.org Build 68)About This Section Paralogs for GNAI2 gene GNAQ 2 GNA14 2 GNA11 2 GNAI3 2 GNAT2 2 GNA13 2 GNAT3 2 GNAT1 2 GNA15 2 GNA12 2 GNAZ 2 GNAI1 2 GNAO1 2 18 SIMAP similar genes for GNAI2 using alignment to 8 protein entries: GNAI2_HUMAN (see all proteins ):GNAI3 GNAI1 GNAT3 GNAT2 DKFZp686O0962 GNAT1 GNAZ GNAO1 GNA11 GNA14 GNAQ GNA12 GNA13 GNAL GNA15 GNAS GSA WUGSC About GeneDecksing 2 Pseudogenes.org Pseudogenes for GNAI2 PGOHUM00000238547 PGOHUM00000239685
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Genomic Variantsfor GNAI2 gene (SNPs/Variants according to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE ,
UniProtKB , and
DNA2.0 ,
Linkage Disequilibrium by HapMap ,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants , Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB) , Blood group antigen gene mutations by BGMUT ,
Resequencing Primers from QIAGEN ,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences )
About This Section Genomic Data Transcription Related Data Allele Frequencies SNP ID Valid Clinical Chr 3 pos Sequence # AA Type More # Allele Pop Total More
summary
for 
Expression by the Database of Embryonic development, Stem cell research, and
Regenerative medicine 
Anatomical Compartment 
Negative
Selective marker
Curated
Microarrays