Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



GFI1 Gene

protein-coding   GIFtS: 65
GCID: GC01M092940

Growth Factor Independent 1 Transcription Repressor

(Previous name: growth factor independent 1)
(Previous symbol: ZNF163)
  See GFI1-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Growth Factor Independent 1 Transcription Repressor1 2     Growth Factor Independent 11
ZNF1631 2 3 5     GFI-12
Growth Factor Independent Protein 12 3     GFI1A2
Zinc Finger Protein 1632 3     Growth Factor Independence-12
SCN22 5     Zinc Finger Protein Gfi-12

External Ids:    HGNC: 42371   Entrez Gene: 26722   Ensembl: ENSG000001626767   OMIM: 6008715   UniProtKB: Q996843   

Export aliases for GFI1 gene to outside databases

Previous GC identifers: GC01M093408 GC01M092149 GC01M092277 GC01M092412 GC01M092652 GC01M092712 GC01M091061


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for GFI1 Gene:
This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays
a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a
complex along with other cofactors to control histone modifications that lead to silencing of the target gene
promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant
nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause
predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein,
have been identified for this gene. (provided by RefSeq, Jul 2008)

GeneCards Summary for GFI1 Gene:
GFI1 (growth factor independent 1 transcription repressor) is a protein-coding gene. Diseases associated with GFI1 include severe congenital neutropenia, and neutropenia. GO annotations related to this gene include transcription regulatory region DNA binding. An important paralog of this gene is GFI1B.

UniProtKB/Swiss-Prot: GFI1_HUMAN, Q99684
Function: Transcription repressor essential for hematopoiesis. Functions in a cell-context and
development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes.
Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC
complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in
multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid
cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of
CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR)
inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth.
Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of
GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells

Gene Wiki entry for GFI1 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000001.10  NT_032977.10  NC_018912.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the GFI1 gene promoter:
         AML1a   STAT3   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): GFI1 promoter sequence
   Search Chromatin IP Primers for GFI1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat GFI1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p22   Ensembl cytogenetic band:  1p22.1   HGNC cytogenetic band: 1p22

GFI1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GFI1 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M092940:  view genomic region     (about GC identifiers)

Start:
92,940,318 bp from pter      End:
92,952,433 bp from pter
Size:
12,116 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: GFI1_HUMAN, Q99684 (See protein sequence)
Recommended Name: Zinc finger protein Gfi-1  
Size: 422 amino acids; 45297 Da
Subunit: Interacts with U2AF1L4. Component of RCOR-GFI-KDM1A-HDAC complexes. Interacts directly with RCOR1, KDM1A
and HDAC2 (By similarity). Also interacts with HDAC1. Interacts (via the zinc-finger domain) with ARIH2; the
interaction prevents GFI1 ubiquitination and proteasomal degradation. Interacts with PIAS3; the interaction
relieves the inhibitory effect of PIAS3 on STAT3-mediated transcriptional activity. Forms a complex with EHMT2
and HDAC1 to promote 'Lys-9' dimethylation of H3 (H3K9Me2) and repress expression of target genes. Interacts
directly with EHMT2. Component of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly with AJUBA (via ITS
LIM domains); the interaction results in the HDAC-dependent corepression of a subset of GFI1 target genes and,
occurs independently of the SNAG domain. Interacts with SPI1; the interaction inhibits SPI1 transcriptional
activity and represses SPI1-regulated macrophage-specific genes required for proper granulocyte development.
Interacts with RUNX1T1; the interaction represses HDAC-mediated transcriptional activity. Interacts with RELA;
the interaction occurs on liposaccharide (LPS) stimulation and controls RELA DNA binding activity and regulates
endotoxin-mediated TOLL-like receptor inflammatory response. Interacts (via the C-terminal zinc fingers) with
ZBTB17; the interaction results in the recruitment of GFI1 to the CDKN1A/p21 and CDKN1B promoters and repression
of transcription
Secondary accessions: Q8N564

Explore the universe of human proteins at neXtProt for GFI1: NX_Q99684

Explore proteomics data for GFI1 at MOPED

Post-translational modifications: 

  • Ubiquitinated. Ubiquitination and degradation by the proteasome is inhibited by the ubiquitin ligase, ARIH21
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See GFI1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001120687.1  NP_001120688.1  NP_005254.2  

    ENSEMBL proteins: 
     ENSP00000359357   ENSP00000294702   ENSP00000399719  

    GFI1 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for GFI1
    OriGene Protein Over-expression Lysate for GFI1
    OriGene Custom MassSpec
    OriGene Custom Protein Services for GFI1
    GenScript Custom Purified and Recombinant Proteins Services for GFI1
    Novus Biologicals GFI1 Protein
    Novus Biologicals GFI1 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for GFI1

     
    Search eBioscience for Proteins for GFI1 

     
    antibodies-online proteins for GFI1 (5 products) 

     
    Search antibodies-online for peptides for GFI1

    GFI1 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of GFI1
    R&D Systems Antibodies for GFI1 (GFI-1)
    OriGene Antibodies for GFI1
    OriGene Custom Antibody Services for GFI1
    Novus Biologicals GFI1 Antibodies
    Search for Antibodies for GFI1 at Abcam
    Cloud-Clone Corp. Antibodies for GFI1
    ThermoFisher Antibodies for GFI1
    antibodies-online antibodies for GFI1 (46 products) 

    GFI1 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for GFI1
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for GFI1
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for GFI1
    Cloud-Clone Corp. CLIAs for GFI1
    Search eBioscience for ELISAs for GFI1 
    antibodies-online kits for GFI1 (8 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    ZNF: Zinc fingers, C2H2-type

    4 InterPro protein domains:
     IPR015880 Znf_C2H2-like
     IPR013087 Znf_C2H2/integrase_DNA-bd
     IPR007087 Znf_C2H2
     IPR001878 Znf_CCHC

    Graphical View of Domain Structure for InterPro Entry Q99684

    ProtoNet protein and cluster: Q99684

    1 Blocks protein domain: IPB007086 C2H2-type zinc finger signature

    UniProtKB/Swiss-Prot: GFI1_HUMAN, Q99684
    Domain: Zinc fingers 3, 4 and 5 are required for DNA-binding and for interaction with SPI1
    Domain: The SNAG domain of GFIs is required for nuclear location and for interaction with some corepressors (By
    similarity)
    Similarity: Contains 6 C2H2-type zinc fingers


    Find genes that share domains with GFI1           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: GFI1_HUMAN, Q99684
    Function: Transcription repressor essential for hematopoiesis. Functions in a cell-context and
    development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes.
    Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC
    complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in
    multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid
    cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of
    CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR)
    inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth.
    Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of
    GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells
    Induction: Down-regulated by TGFB1

         Genatlas biochemistry entry for GFI1:
    growth factor independence 1 (independent from IL2 for growth),expressed in bone marrow,associated with a
    constitutional translocation from a patient with stage 4S neuroblastoma

         Gene Ontology (GO): 5 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001078RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription IDA19506020
    GO:0005515protein binding IPI11060035
    GO:0008270zinc ion binding IEA--
    GO:0044212transcription regulatory region DNA binding IDA15947108
    GO:0046872metal ion binding ----
         
    Find genes that share ontologies with GFI1           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for GFI1:
     Decreased viability with pacli 

         8 MGI mutant phenotypes (inferred from 11 alleles(MGI details for Gfi1):
     behavior/neurological  cellular  growth/size/body  hearing/vestibular/ear  hematopoietic system 
     immune system  mortality/aging  nervous system 

    Find genes that share phenotypes with GFI1           About GenesLikeMe

    Animal Models:
         MGI mouse knock-outs for GFI1: Gfi1tm1Sho Gfi1tm1.1Wep Gfi1tm1Tmo

       genOway: Develop your customized and physiologically relevant rodent model for GFI1

    Transcription Factor Targeting: 
    Selected GeneGlobe predicted Target genes for GFI1 (see all 414):
    ABCC12,  ACAD9,  ACADS,  ACOT8,  ACOX1,  ADCK1,  ADO,  ADORA2B,  AGFG2,  AGPAT4

    miRNA
    Products:
        
    Block miRNA regulation of human, mouse, rat GFI1 using miScript Target Protectors
    8 qRT-PCR Assays for microRNAs that regulate GFI1:
    hsa-miR-142-3p hsa-miR-495 hsa-miR-429 hsa-miR-4255 hsa-miR-3924 hsa-miR-877* hsa-miR-200c hsa-miR-200b
    SwitchGear 3'UTR luciferase reporter plasmidGFI1 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for GFI1
    Predesigned siRNA for gene silencing in human, mouse, rat GFI1

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for GFI1

    Clone
    Products:
         
    OriGene clones in human, mouse for GFI1 (see all 17)
    OriGene ORF clones in mouse, rat for GFI1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): GFI1 (NM_001127216)
    Sino Biological Human cDNA Clone for GFI1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for GFI1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GFI1
    Addgene plasmids for GFI1 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for GFI1
    Browse ESI BIO Cell Lines and PureStem Progenitors for GFI1 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GFI1


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    GFI1_HUMAN, Q99684: Nucleus. Note=Colocalizes with PIAS3 and RUNX1T1 in nuclear dots
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA12874834
    GO:0016363nuclear matrix IDA12874834
    GO:0016604nuclear body IDA11060035
    GO:0017053transcriptional repressor complex IDA12874834

    Find genes that share ontologies with GFI1           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for GFI1 About    
    See pathways by source

    SuperPathContained pathways About
    1Validated targets of C-MYC transcriptional repression
    Validated targets of C-MYC transcriptional repression


    1 BioSystems Pathway for GFI1
        Validated targets of C-MYC transcriptional repression



        Pathway & Disease-focused RT2 Profiler PCR Arrays including GFI1: 
              Th1/Th2 Response in human mouse rat
              T Helper Cell Differentiation in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for GFI1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for GFI1 (Q996841, 2, 3 ENSP000002947024) via UniProtKB, MINT, STRING, and/or I2D (see all 55)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ENSG00000206376Q96KQ71, 3EBI-949368,EBI-744366 I2D: score=2 
    ENSG00000232045Q96KQ71, 3EBI-949368,EBI-744366 I2D: score=2 
    ENSG00000236759Q96KQ71, 3EBI-949368,EBI-744366 I2D: score=2 
    ENSG00000238134Q96KQ71, 3EBI-949368,EBI-744366 I2D: score=2 
    PRDM5Q9NQX11, 3, ENSP000002648084EBI-949368,EBI-4292031 I2D: score=2 STRING: ENSP00000264808
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 18):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000083regulation of transcription involved in G1/S transition of mitotic cell cycle TAS8441411
    GO:0000122negative regulation of transcription from RNA polymerase II promoter IDA15947108
    GO:0006351transcription, DNA-templated ----
    GO:0007638mechanosensory behavior IEA--
    GO:0009996negative regulation of cell fate specification IEA--

    Find genes that share ontologies with GFI1           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for GFI1

    3 Novoseek inferred chemical compound relationships for GFI1 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    zinc 48.8 9 18805794 (1), 12579345 (1), 11246463 (1), 7789186 (1) (see all 6)
    calcitriol 21.4 1 17207994 (1)
    oligonucleotide 18.9 7 8754800 (1), 15254021 (1), 11503967 (1)



    Find genes that share compounds with GFI1           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for GFI1 gene (3 alternative transcripts): 
    NM_001127215.1  NM_001127216.1  NM_005263.3  

    Unigene Cluster for GFI1:

    Growth factor independent 1 transcription repressor
    Hs.73172  [show with all ESTs]
    Unigene Representative Sequence: AK123009
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000370332(uc001dou.4) ENST00000294702(uc001dow.4) ENST00000483490
    ENST00000427103(uc001dov.4)
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat GFI1 using miScript Target Protectors
    8 qRT-PCR Assays for microRNAs that regulate GFI1:
    hsa-miR-142-3p hsa-miR-495 hsa-miR-429 hsa-miR-4255 hsa-miR-3924 hsa-miR-877* hsa-miR-200c hsa-miR-200b
    SwitchGear 3'UTR luciferase reporter plasmidGFI1 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for GFI1
    Predesigned siRNA for gene silencing in human, mouse, rat GFI1
    Clone
    Products:
         
    OriGene clones in human, mouse for GFI1 (see all 17)
    OriGene ORF clones in mouse, rat for GFI1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): GFI1 (NM_001127216)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for GFI1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GFI1
    Addgene plasmids for GFI1 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for GFI1
    OriGene qSTAR qPCR primer pairs in human, mouse for GFI1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat GFI1
      QuantiTect SYBR Green Assays in human, mouse, rat GFI1
      QuantiFast Probe-based Assays in human, mouse, rat GFI1

    Additional mRNA sequence: 

    AB209606.1 AK123009.1 AK313876.1 BC032751.1 BC074866.2 BC074867.2 U67369.1 

    5 DOTS entries:

    DT.409203  DT.101958211  DT.99935049  DT.95364989  DT.121331690 

    Selected AceView cDNA sequences (see all 34):

    BC074867 BC074866 BX103897 AA418008 AW338888 AA932727 AW515390 BG150508 
    AW338887 BF445059 BE669987 U67369 AK123009 BM920225 BX436829 NM_005263 
    BC032751 BE670371 CD520401 AA418106 BF432914 BE045631 BM479949 BM463411 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    GFI1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TGTACCATAG
    GFI1 Expression
    About this image


    GFI1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 8) fully expand
     
     Blood (Cardiovascular System)    fully expand to see all 9 entries
             Common Lymphoid Progenitor Cells Hematopoietic Bone Marrow
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Hippocampus
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Liver (Hepatobiliary System)    fully expand to see all 2 entries
             Hematopoietic Stem Cells Liver Bud
     
     Bone (Muscoskeletal System)    fully expand to see all 2 entries
             Bone Marrow
    GFI1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    GFI1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.73172
        Pathway & Disease-focused RT2 Profiler PCR Arrays including GFI1: 
              Th1/Th2 Response in human mouse rat
              T Helper Cell Differentiation in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for GFI1
    OriGene qSTAR qPCR primer pairs in human, mouse for GFI1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat GFI1
    QuantiTect SYBR Green Assays in human, mouse, rat GFI1
    QuantiFast Probe-based Assays in human, mouse, rat GFI1
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GFI1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for GFI1 gene from Selected species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Gfi11 , 5 growth factor independent 11, 5 84.76(n)1
    87.68(a)1
      5 (52.23 cM)5
    145811  NM_010278.21  NP_034408.11 
     1077166575 
    chicken
    (Gallus gallus)
    Aves GFI11 growth factor independent 1 transcription repressor 73.91(n)
    79.8(a)
      429088  XM_426644.4  XP_426644.3 
    lizard
    (Anolis carolinensis)
    Reptilia GFI16
    growth factor independent 1 transcription represso...
    76(a)
    1 ↔ 1
    4(93699778-93722805)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia gfi11 growth factor independent 1 transcription repressor 69.03(n)
    74.8(a)
      100491774  XM_002933803.2  XP_002933849.1 
    zebrafish
    (Danio rerio)
    Actinopterygii gfi1ab1 growth factor independent 1ab 64.87(n)
    70.26(a)
      798697  NM_201338.1  NP_958495.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Ly3 photoreceptor morphogenesis (sensu
    Drosophila) more
    86(a)   3 70A8   --
    worm
    (Caenorhabditis elegans)
    Secernentea pag-33 Zinc finger, C2H2 type (5 domains) 76(a)   X(14986422-14989743)   --


    ENSEMBL Gene Tree for GFI1 (if available)
    TreeFam Gene Tree for GFI1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for GFI1 gene
    GFI1B2  ZNF82  FEZF12  FEZF22  
    Selected SIMAP similar genes for GFI1 using alignment to 2 protein entries:     GFI1_HUMAN (see all proteins) (see all similar genes):
    GFI1B    ZNF78L1    ZNF818P    DKFZp686O111    ZNF55    ZNF667
    ZNF    ZFS-5    DKFZp686M04222    ZNF235    ZNF253    ZNF493
    ZNF726    DKFZp434J0650    ZKSCAN4    ZNF135    ZNF211    ZNF22

    Find genes that share paralogs with GFI1           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for GFI1 (see all 303)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs289363821,2,,4
    C,FDominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA)4 pathogenic192962973(-) TGGGAA/GGGGTT 6 K R mis11Minor allele frequency- G:0.00NA 4550
    rs289363811,2,,4
    CNeutropenia, severe congenital 2, autosomal dominant (SCN2)4 pathogenic192963036(-) CTCCAA/GCCTCA 6 N S mis1 ese30--------
    rs358964851,2
    C--92944351(+) GAGAG-/A/G   
       AGAGA
    TGCAG
    6 -- int1 cds11NA 2
    rs1427570101,2
    C--92947516(+) GCACA-/C/CACACAC/C
    GCGCACACACACAC
    ACACT
    6 -- int1 cds10--------
    rs1500370861,2
    --92952080(+) ACCCG-/CCCCCC 2 -- int1 us2k10--------
    rs118051551,2
    C,H--92952218(+) CCCGCA/GGGCCC 2 -- int1 us2k10--------
    rs666352551,2
    C--92952218(+) CCCGC-/AGGCCC 2 -- int1 us2k11Minor allele frequency- A:0.50CSA 2
    rs57761461,2
    C,F--92953144(+) GTTATG/-GGGGG 2 -- us2k1 trp32Minor allele frequency- -:0.50NA CSA 4
    rs772260441,2
    F--92961237(+) ATGATG/ATGTTG 3 -- ds50011Minor allele frequency- A:0.04WA 118
    rs583941611,2
    C,F--92961285(+) AGACCT/CGTGAT 3 -- ds50014Minor allele frequency- C:0.14NA CSA WA 242

    HapMap Linkage Disequilibrium report for GFI1 (92940318 - 92952433 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for GFI1:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2714684CNV Deletion23290073
    nsv523354CNV Gain19592680

    Human Gene Mutation Database (HGMD): GFI1
    Locus Specific Mutation Databases (LSDB): GFI1

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing GFI1
    DNA2.0 Custom Variant and Variant Library Synthesis for GFI1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 600871   
    OMIM disorders: 613107  607847  
    UniProtKB/Swiss-Prot: GFI1_HUMAN, Q99684
  • Neutropenia, severe congenital 2, autosomal dominant (SCN2) [MIM:613107]: A disorder of hematopoiesis
    characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute
    neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Note=The disease is caused
    by mutations affecting the gene represented in this entry
  • Dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]: Relatively mild form
    of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients. Note=The disease is
    caused by mutations affecting the gene represented in this entry

  • 9 diseases for GFI1:    
    About MalaCards
    severe congenital neutropenia    neutropenia    severe congenital neutropenia, autosomal dominant, 2    tetraploidy
    idiopathic neutropenia    reticular dysgenesis    neutropenia, severe congenital 1, autosomal dominant    splenic marginal zone lymphoma
    myeloproliferative disorder

    1 disease from the University of Copenhagen DISEASES database for GFI1:
    Neutropenia

    Find genes that share disorders with GFI1           About GenesLikeMe

    7 Novoseek inferred disease relationships for GFI1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    neutropenia, severe congenital 90.9 9 16500901 (2), 19775295 (2), 16716599 (1), 19346496 (1) (see all 5)
    neutropenia 58.5 13 18328744 (2), 17636019 (2), 18334177 (1), 20008220 (1) (see all 9)
    leukemia 8.33 1 16716599 (1)
    tumors 0 5 16716599 (2), 11246463 (1), 17978002 (1), 8887656 (1)
    prostate cancer 0 7 17207994 (3), 15947108 (2)
    lymphoma 0 1 15231650 (1)
    cancer 0 1 12525640 (1)

    Genetic Association Database (GAD): GFI1
    Human Genome Epidemiology (HuGE) Navigator: GFI1 (3 documents)
    Tumor Gene Database (TGDB): GFI1

    Export disorders for GFI1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for GFI1 gene, integrated from 10 sources (see all 96):
    (articles sorted by number of sources associating them with GFI1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Gfi1 ubiquitination and proteasomal degradation is inhibited by the ubiquitin ligase Triad1. (PubMed id 17646546)1, 2, 9 Marteijn J.A.... Van der Reijden B.A. (Blood 2007)
    2. Gfi1 coordinates epigenetic repression of p21Cip/WAF1 by recruitment of histone lysine methyltransferase G9a and histone deacetylase 1. (PubMed id 16287849)1, 2, 9 Duan Z.... Horwitz M. (Mol. Cell. Biol. 2005)
    3. Gfi-1 represses CDKN2B encoding p15INK4B through interaction with Miz-1. (PubMed id 19164764)1, 2, 9 Basu S.... Dong F. (Proc. Natl. Acad. Sci. U.S.A. 2009)
    4. Ajuba functions as a histone deacetylase-dependent co-repressor for autoregulation of the growth factor-independent-1 transcription factor. (PubMed id 18805794)1, 2, 9 Montoya-Durango D.E.... Grimes H.L. (J. Biol. Chem. 2008)
    5. Gfi-1 attaches to the nuclear matrix, associates with ETO (MTG8) and histone deacetylase proteins, and represses transcription using a TSA- sensitive mechanism. (PubMed id 12874834)1, 2, 9 McGhee L.... Meyers S. (J. Cell. Biochem. 2003)
    6. Gfi-1 encodes a nuclear zinc finger protein that binds DNA and functions as a transcriptional repressor. (PubMed id 8754800)1, 2, 9 Zweidler-Mckay P.A.... Tsichlis P.N. (Mol. Cell. Biol. 1996)
    7. A variant allele of Growth Factor Independence 1 (GFI1) is associated with acute myeloid leukemia. (PubMed id 20075157)1, 4, 9 Khandanpour C....MAPrAPy T. (Blood 2010)
    8. Chromosomal localization of a gene, GF1, encoding a novel zinc finger protein reveals a new syntenic region between man and rodents. (PubMed id 7789186)1, 3, 9 Bell D.W....Testa J.R. (Cytogenet. Cell Genet. 1995)
    9. Loss of function genetic screens reveal MTGR1 as an intracellular repressor of beta1 integrin-dependent neurite outgrowth. (PubMed id 19026687)1, 2, 9 Ossovskaya V.S.... Basbaum A.I. (J. Neurosci. Methods 2009)
    10. Zinc finger protein Gfi1 controls the endotoxin-mediated Toll-like receptor inflammatory response by antagonizing NF-kappaB p65. (PubMed id 20547752)1, 2 Sharif-Askari E....Moroy T. (Mol. Cell. Biol. 2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 2672 HGNC: 4237 AceView: GFI1 Ensembl:ENSG00000162676 euGenes: HUgn2672
    ECgene: GFI1 H-InvDB: GFI1

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for GFI1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for GFI1 Genetics and Cytogenetics in Oncology and Haematology
    GFI1basehttp://bioinf.uta.fi/GFI1base/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for GFI1 gene:
    Search GeneIP for patents involving GFI1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from genOway)
    About This Section

    TryGeneCards Plus

     Browse Small Molecules at EMD Millipore
     Browse Purified and Recombinant Proteins at EMD Millipore
     Browse Kits and Assays available from EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of GFI1
     
     EMD Millipore genomic analysis products

      
     Antibodies for GFI1 (GFI-1)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for GFI1   OriGene RNAi products in human, mouse, rat for GFI1  
     OriGene qPCR primer pairs and template standards for GFI1   OriGene Protein Over-expression Lysate for GFI1  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for GFI1  
     OriGene qSTAR qPCR primer pairs in human, mouse for GFI1   OriGene Purified Protein for GFI1  
     OriGene ORF clones in mouse, rat for GFI1   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for GFI1   OriGene Custom Protein Services for GFI1  

     
     
     Block miRNA regulation of human, mouse, rat GFI1 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing GFI1
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat GFI1 Predesigned siRNA for gene silencing in human, mouse, rat GFI1
     QuantiFast Probe-based Assays in human, mouse, rat GFI1 QuantiTect SYBR Green Assays in human, mouse, rat GFI1
     PCR Arrays including human, mouse, rat GFI1 Search Chromatin IP Primers for GFI1
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat GFI1  GeneGlobe Interaction Network for GFI1
     Regulatory tfbs in GFI1 promoter
     GenScript Custom Purified and Recombinant Proteins Services for GFI1 GenScript cDNA clones with any tag delivered in your preferred vector for GFI1
     GenScript Custom Assay Services for GFI1 GenScript Custom overexpressing Cell Line Services for GFI1
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Search Tocris compounds for GFI1
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     cDNA Clones for GFI1
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     GFI1 antibodies
     GFI1 proteins
     GFI1 lysates
     Search for Antibodies for GFI1 at Abcam
     See all of Abcam's Antibodies, Kits and Proteins for GFI1
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for GFI1
     Antibodies for GFI1
     ELISAs for GFI1
     CLIAs for GFI1



     Browse ESI BIO Cell Lines and PureStem Progenitors for GFI1
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for GFI1
     SwitchGear 3'UTR luciferase reporter plasmids for GFI1
     SwitchGear Promoter luciferase reporter plasmids for GFI1
     ThermoFisher Antibodies for GFI1
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GFI1
     Browse compounds at ApexBio
     Addgene plasmids for GFI1
      Search eBioscience for proteins for GFI1
      Search eBioscience for elisas for GFI1
      eBioscience FlowRNA Probe Sets
     genOway: Develop your customized and physiologically relevant rodent model for GFI1
     antibodies-online antibodies for GFI1 (46 products)
     antibodies-online kits for GFI1 (8 products)
      Search antibodies-online for peptides for GFI1
     antibodies-online proteins for GFI1 (5 products)
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014 , 7 Oct 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      GFI1 gene at Home site.
    Version: 3.12.248 16 Nov 2014
    hostname: 356977-web1.xennexinc.com index build: 128 solr: 1.4