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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

GFI1 Gene

protein-coding GIFtS: 64
GCID: GC01M092940

growth factor independent 1 transcription repressor

(Previous name: growth factor independent 1 )
(Previous symbol: ZNF163)

Explore 23 diseases affiliated with
GFI1 via

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(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Growth Factor Independent 1 Transcription Repressor¹ ²		Zinc Finger Protein 163² ³
ZNF163¹ ² ³ ⁵		SCN2² ⁵
GFI-1¹ ²		Growth Factor Independent 1¹
GFI1A¹ ²		Growth Factor Independence-1²
Growth Factor Independent Protein 1² ³		Zinc Finger Protein Gfi-1²

External Ids:	HGNC: 4237¹	Entrez Gene: 2672²	Ensembl: ENSG00000162676⁷	OMIM: 600871⁵	UniProtKB: Q99684³

Export aliases for GFI1 gene to outside databases

Previous GC identifers: GC01M093408 GC01M092149 GC01M092277 GC01M092412 GC01M092652 GC01M092712 GC01M091061

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for GFI1:

This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a

role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex

along with other cofactors to control histone modifications that lead to silencing of the target gene promoters.

Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic

idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to

leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for

this gene. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: GFI1_HUMAN, Q99684

Function: Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific

manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several

complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via

histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates

neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development.

Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates

the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to

regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in

response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the

maintenance of inner ear hair cells

Gene Wiki entry for GFI1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000001.10 NC_018912.1 NT_032977.9

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the GFI1 gene promoter:
AML1a STAT3
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): GFI1 promoter sequence

Search SABiosciences Chromatin IP Primers for GFI1

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GFI1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p22 Ensembl cytogenetic band: 1p22.1 HGNC cytogenetic band: 1p22

GFI1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GFI1 gene location

GeneLoc information about chromosome 1 GeneLoc Exon Structure

GeneLoc location for GC01M092940: view genomic region (about GC identifiers)

Start:	92,940,318 bp from pter	End:	92,952,433 bp from pter
Size:	12,116 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: GFI1_HUMAN, Q99684 (See protein sequence)

Recommended Name: Zinc finger protein Gfi-1

Size: 422 amino acids; 45297 Da

Subunit: Interacts with U2AF1L4. Component of RCOR-GFI-KDM1A-HDAC complexes. Interacts directly with RCOR1, KDM1A and

HDAC2 (By similarity). Also interacts with HDAC1. Interacts (via the zinc-finger domain) with ARIH2; the interaction

prevents GFI1 ubiquitination and proteasomal degradation. Interacts with PIAS3; the interaction relieves the

inhibitory effect of PIAS3 on STAT3-mediated transcriptional activity. Forms a complex with EHMT2 and HDAC1 to promote

'Lys-9' dimethylation of H3 (H3K9Me2) and repress expression of target genes. Interacts directly with EHMT2. Component

of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly with AJUBA (via ITS LIM domains); the interaction

results in the HDAC-dependent corepression of a subset of GFI1 target genes and, occurs independently of the SNAG

domain. Interacts with SPI1; the interaction inhibits SPI1 transcriptional activity and represses SPI1-regulated

macrophage-specific genes required for proper granulocyte development. Interacts with RUNX1T1; the interaction

represses HDAC-mediated transcriptional activity. Interacts with RELA; the interaction occurs on liposaccharide (LPS)

stimulation and controls RELA DNA binding activity and regulates endotoxin-mediated TOLL-like receptor inflammatory

response. Interacts (via the C-terminal zinc fingers) with ZBTB17; the interaction results in the recruitment of GFI1

to the CDKN1A/p21 and CDKN1B promoters and repression of transcription

Subcellular location: Nucleus. Note=Colocalizes with PIAS3 and RUNX1T1 in nuclear dots

Secondary accessions: Q8N564

Explore the universe of human proteins at neXtProt for GFI1: NX_Q99684

Post-translational modifications:

Ubiquitinated. Ubiquitination and degradation by the proteasome is inhibited by the ubiquitin ligase, ARIH2¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q99684

GFI1 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (3 alternative transcripts):

NP_001120687.1 NP_001120688.1 NP_005254.2

ENSEMBL proteins:

ENSP00000359357 ENSP00000294702 ENSP00000399719

Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene Protein Over-expression Lysate (see all 3): GFI1 OriGene Custom Protein Services for GFI1
	GenScript Custom Purified and Recombinant Proteins Services for GFI1
	Novus Biologicals GFI1 Protein Novus Biologicals GFI1 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for GFI1

Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	12874834
GO:0016363	nuclear matrix	IDA	12874834
GO:0016604	nuclear body	IDA	11060035
GO:0017053	transcriptional repressor complex	IDA	12874834

GFI1 for ontologies About GeneDecksing

GFI1 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of GFI1
	R&D Systems Antibodies for GFI1 (GFI-1)
	Browse OriGene Antibodies OriGene Custom Antibody Services for GFI1
	GenScript Custom Superior Antibodies Services for GFI1
	Novus Biologicals GFI1 Antibodies
	Search for Antibodies for GFI1 at Abcam
	Uscn Antibodies for GFI1
	ThermoFisher Antibodies for GFI1

Assay Products for GFI1:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for GFI1
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for GFI1

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

GFI1 for domains About GeneDecksing

4 InterPro domains/families:

IPR015880 Znf_C2H2-like

IPR013087 Znf_C2H2/integrase_DNA-bd

IPR007087 Znf_C2H2

IPR001878 Znf_CCHC

Graphical View of Domain Structure for InterPro Entry Q99684

ProtoNet protein and cluster: Q99684

1 Blocks protein family: IPB007086 C2H2-type zinc finger signature

UniProtKB/Swiss-Prot: GFI1_HUMAN, Q99684

Domain: Zinc fingers 3, 4 and 5 are required for DNA-binding and for interaction with SPI1

Domain: The SNAG domain of GFIs is required for nuclear location and for interaction with some corepressors (By

similarity)

Similarity: Contains 6 C2H2-type zinc fingers

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary: