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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

GAL3ST4 Gene

protein-coding   GIFtS: 52
GCID: GC07M099760

galactose-3-O-sulfotransferase 4

 Explore 2 diseases affiliated with
GAL3ST4 via our new
 Human Malady Compendium 
Biological research products
for GAL3ST4
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Galactose-3-O-Sulfotransferase 41 2     Beta-Galactose-3-O-Sulfotransferase, 42
Beta-Galactose-3-O-Sulfotransferase 42 3     Galbeta1-3GalNAc 3'-Sulfotransferase2
Gal-Beta-1,3-GalNAc 3'-Sulfotransferase2 3     EC 2.8.2.-3
FLJ121161     Gal3ST-43
GAL3ST-42     

External Ids:    HGNC: 241451   Entrez Gene: 796902   Ensembl: ENSG000001970937   OMIM: 6082355   UniProtKB: Q96RP73   

Export aliases for GAL3ST4 gene to outside databases

Previous GC identifers: GC07M099369 GC07M099401 GC07M099594 GC07M099757 GC07M094391 GC07M099758


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for GAL3ST4:
This gene encodes a member of the galactose-3-O-sulfotransferase protein family. The product of this gene catalyzes
sulfonation by transferring a sulfate to the C-3' position of galactose residues in O-linked glycoproteins. This
enzyme is highly specific for core 1 structures, with asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3
(GlcNAc-beta-1,6)GalNAc being good substrates. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: G3ST4_HUMAN, Q96RP7
Function: Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good
substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc

Gene Wiki entry for GAL3ST4


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000007.13  NC_018918.1  NT_007933.15  NT_079595.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the GAL3ST4 gene promoter:
         Max1   E2F-4   E2F-3a   E2F-5   Sp1   E2F-2   AREB6   E2F   E2F-1   c-Myc   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): GAL3ST4 promoter sequence
   Search SABiosciences Chromatin IP Primers for GAL3ST4

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GAL3ST4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7q22.1   Ensembl cytogenetic band:  7q22.1   HGNC cytogenetic band: 7q22.1

GAL3ST4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GAL3ST4 gene location

GeneLoc information about chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M099760:  view genomic region     (about GC identifiers)

Start:
99,756,865 bp from pter      End:
99,766,373 bp from pter
Size:
9,509 bases      Orientation:
minus strand

1 alternative location:
Chr7-,CRA_TCAG 99,116,951-99,126,460     

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: G3ST4_HUMAN, Q96RP7 (See protein sequence)
Recommended Name: Galactose-3-O-sulfotransferase 4  
Size: 486 amino acids; 54166 Da
Cofactor: Manganese
Subcellular location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein (Probable)
Secondary accessions: A4D2A8 D6W5U5 Q8N3P7 Q8WZ17 Q96E33 Q9HA78

Explore the universe of human proteins at neXtProt for GAL3ST4: NX_Q96RP7

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96RP7

  • GAL3ST4 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_078913.3  
    ENSEMBL proteins: 
     ENSP00000400451   ENSP00000353142   ENSP00000399066   ENSP00000414733   ENSP00000398304  

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    Uscn Proteins for GAL3ST4

    Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005624membrane fraction ----
    GO:0005794Golgi apparatus ----
    GO:0016020membrane IDA11333265
    GO:0016021integral to membrane NAS11333265
    GO:0032580Golgi cisterna membrane IEA--


    GAL3ST4 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    GAL3ST4 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR009729 Gal-3-0_sulfotransfrase

    Graphical View of Domain Structure for InterPro Entry Q96RP7

    ProtoNet protein and cluster: Q96RP7

    1 Blocks protein family: IPB009729 Galactose-3-O-sulfotransferase

    UniProtKB/Swiss-Prot: G3ST4_HUMAN, Q96RP7
    Similarity: Belongs to the galactose-3-O-sulfotransferase family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: G3ST4_HUMAN, Q96RP7
    Function: Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good
    substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc
    Biophysicochemical properties: pH dependence: Optimum pH is 6-7;

    Enzyme Number (IUBMB): EC 2.8.2.-1

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    hsa-miR-607 hsa-miR-1298 hsa-miR-495 hsa-miR-376b hsa-miR-539 hsa-miR-4272 hsa-miR-3065-5p hsa-miR-376a
    SwitchGear 3'UTR luciferase reporter plasmidGAL3ST4 3' UTR sequence
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    Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001733galactosylceramide sulfotransferase activity IEA--
    GO:00506563'-phosphoadenosine 5'-phosphosulfate binding NAS11333265
    GO:0050694galactose 3-O-sulfotransferase activity IDA11333265
    GO:0050698proteoglycan sulfotransferase activity NAS11333265


    GAL3ST4 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for GAL3ST4:
     Decreased viability with pacli 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Biological oxidations
    metapathway biotransformation0.41


    1 BioSystems Pathway for GAL3ST4 
        metapathway biotransformation


    UniProtKB/Swiss-Prot: G3ST4_HUMAN, Q96RP7
    Pathway: Protein modification; carbohydrate sulfation

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for GAL3ST4

    STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

    1 Interacting protein for GAL3ST4 (Q96RP73 ENSP000003531424) via UniProtKB, MINT, STRING, and/or I2D

    InteractantInteraction Details
    GeneCardExternal ID(s)
    XRCC6P129563, ENSP000003522574I2D: score=3 STRING: ENSP00000352257
    About this table

    Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006790sulfur compound metabolic process NAS11333265
    GO:0007267cell-cell signaling NAS11333265
    GO:0009058biosynthetic process ----
    GO:0009100glycoprotein metabolic process NAS11333265
    GO:0009311oligosaccharide metabolic process NAS11333265


    GAL3ST4 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for GAL3ST4

    1 HMDB Compound for GAL3ST4    About this table
    CompoundSynonyms CAS #PubMed Ids
    Manganesemanganese 7439-96-5--
    Search CenterWatch for drugs/clinical trials and news about GAL3ST4 / G3ST4 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for GAL3ST4 gene: 
    NM_024637.4  

    Unigene Cluster for GAL3ST4:

    Galactose-3-O-sulfotransferase 4
    Hs.44856  [show with all ESTs]
    Unigene Representative Sequence: AK092367
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000413800(uc022aii.1) ENST00000495882(uc003utt.3) ENST00000360039(uc003utu.3)
    ENST00000423751 ENST00000411994 ENST00000498000 ENST00000482469 ENST00000460995
    ENST00000426974

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    hsa-miR-607 hsa-miR-1298 hsa-miR-495 hsa-miR-376b hsa-miR-539 hsa-miR-4272 hsa-miR-3065-5p hsa-miR-376a
    SwitchGear 3'UTR luciferase reporter plasmidGAL3ST4 3' UTR sequence
    Inhib. RNA
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    Additional cDNA sequence: 

    AF289575.1 AF316113.1 AK022178.1 AK092367.1 AK225890.1 AK301591.1 AL833824.1 BC012976.2 

    7 DOTS entries:

    DT.75180129  DT.100747352  DT.95262028  DT.100015403  DT.102829773  DT.100677082  DT.95156061 

    24/43 AceView cDNA sequences (see all 43):

    BQ002083 BX422894 BQ017828 AK022178 NM_024637 CR605645 AL833824 BX487064 
    BU740118 BM873805 AF316113 AU121412 BQ072499 BG059546 BC012976 BM548325 
    AK092367 BX421694 CB996165 BX421063 BE782818 AF289575 AW961058 BP351834 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for GAL3ST4    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b ^ 3 ^ 4a · 4b · 4c
    SP1:              -                                 
    SP2:              -           -                     
    SP3:                                                


    ECgene alternative splicing isoforms for GAL3ST4

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    GAL3ST4 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TTTGTTGAAT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    GAL3ST4 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    EyeOuter Nuclear LayerMature Rod CellsPhotoreceptors, Retina
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See GAL3ST4 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for GAL3ST4

    SOURCE GeneReport for Unigene cluster: Hs.44856

    UniProtKB/Swiss-Prot: G3ST4_HUMAN, Q96RP7
    Tissue specificity: Expressed mainly in placenta, thymus, testis, ovary, spinal cord, trachea and adrenal gland and at
    low levels in brain, lung, spleen, prostate, small intestine, colon, stomach thyroid and lymph node

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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for GAL3ST4 gene from 3/13 species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves GAL3ST46
    Uncharacterized protein
    42(a)
    1 ↔ 1
    9(5667065-5667901)
    lizard
    (Anolis carolinensis)
    Reptilia GAL3ST46
    --
    44(a)
    1 ↔ 1
    3(30876640-30888324)
    zebrafish
    (Danio rerio)
    Actinopterygii CR848737.46
    --
    39(a)
    1 ↔ 1
    7(27519620-27534119)


    ENSEMBL Gene Tree for GAL3ST4 (if available)
    TreeFam Gene Tree for GAL3ST4 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for GAL3ST4 gene
    GAL3ST22  GAL3ST32  GAL3ST12  

    GAL3ST4 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for GAL3ST4
    PGOHUM00000240572


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/144 NCBI SNPs in GAL3ST4 are shown (see all 144    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 7 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs2000532881,2
    --99758396(+) GCGAGC/TGTAGT 2 T A mis10--------
    rs2021647441,2
    --99758490(+) GAGGTA/GGATTT 2 S syn10--------
    rs1488504741,2
    C,--99758537(+) GTCTCC/GGACAA 2 R G mis10--------
    rs2017155921,2
    --99758541(+) CGGACA/GATGGA 2 I syn10--------
    rs2002661801,2
    --99758558(+) GAAGCC/TGTCAG 2 S G mis10--------
    rs1434724201,2
    --99758648(+) TGCACA/GAGCCA 1 -- int10--------
    rs1875909461,2
    --99758660(+) GTGTCC/TGCTGA 1 -- int10--------
    rs1509856011,2
    --99758761(+) ACTCAA/GTTTGC 1 -- int10--------
    rs1921457951,2
    --99758998(+) CGGGCA/GTGGTG 1 -- int10--------
    rs1408102371,2
    --99759007(+) TGGCTC/TAAGCC 1 -- int10--------

    HapMap Linkage Disequilibrium report for GAL3ST4 (99756865 - 99766373 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for GAL3ST4: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    GAL3ST4 for disorders           About GeneDecksing

    OMIM gene information: 608235    OMIM disorders: --

    2 diseases for GAL3ST4:    About MalaCards
    thyroiditis    prostatitis


    Export disorders for GAL3ST4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for GAL3ST4 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with GAL3ST4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning and characterization of a novel human galactose 3- O-sulfotransferase that transfers sulfate to Galbeta1->3GalNAc residue in O-glycans. (PubMed id 11333265)1, 2, 3, 9 Seko A.... Yamashita K. (2001)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    4. Large-scale cDNA transfection screening for genes related to cancer development and progression. (PubMed id 15498874)1, 2 Wan D....Gu J. (2004)
    5. Human chromosome 7: DNA sequence and biology. (PubMed id 12690205)1, 2 Scherer S.W.... Tsui L.-C. (2003)
    6. The full-ORF clone resource of the German cDNA consortium. (PubMed id 17974005)2 Bechtel S.... Schupp I. (2007)
    7. A human protein-protein interaction network: a resource for annotating the proteome. (PubMed id 16169070)1 Stelzl U.... Wanker E.E. (2005)
    8. Identification of physiologically relevant substrates for cloned Gal: 3-O-sulfotransferases (Gal3STs): distinct high affinity of Gal3ST-2 and LS180 sulfotransferase for the globo H backbone, Gal3ST-3 for N-glycan multiterminal Galbeta1, 4GlcNAcbeta units and 6-sulfoGalbeta1, 4GlcNAcbeta, and Gal3ST-4 for the mucin core-2 trisaccharide. (PubMed id 14701868)1 Chandrasekaran E.V....Matta K.L. (2004)
    9. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
    10. Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. (PubMed id 8125298)1 Maruyama K. and Sugano S. (1994)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 79690 HGNC: 24145 AceView: GAL3ST4 Ensembl:ENSG00000197093 euGenes: HUgn79690
    ECgene: GAL3ST4 H-InvDB: GAL3ST4

    (According to HUGE)
    About This Section
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for GAL3ST4 Pharmacogenomics, SNPs, Pathways
    GGDBhttp://riodb.ibase.aist.go.jp/rcmg/ggdb/

    (Patent information from GeneIP,
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    Patent Information for GAL3ST4 gene:
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