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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

GAGE2A Gene

protein-coding   GIFtS: 39
GCID: GC0XP049360

G antigen 2A

(Previous name: G antigen 2 )
(Previous symbol: GAGE2)
 Explore 2 diseases affiliated with
GAGE2A via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for GAGE2A    

Aliases
for GAGE2A gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
G Antigen 2A1 2     GAGE-22
GAGE21 2 3     Cancer/Testis Antigen 4.22
CT4.21 2     Cancer/Testis Antigen Family 4, Member 22
GAGE-2A2 3     G Antigen 2A/2B2
G Antigen 21     

External Ids:    HGNC: 40991   Entrez Gene: 7294472   Ensembl: ENSG000001890647   OMIM: 3007205   UniProtKB: Q6NT463   

Export aliases for GAGE2A gene to outside databases

Previous GC identifers: GC0XP049117 GC0XP049186 GC0XP049243 GC0XP049244 GC0XP049358


Summaries
for GAGE2A gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
  --

Genomic Views
for GAGE2A gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000023.10  NT_086939.3  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for GAGE2A
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search SABiosciences Chromatin IP Primers for GAGE2A

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat GAGE2A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xp11.23   Ensembl cytogenetic band:  Xp11.23   HGNC cytogenetic band: Xp11.23

GAGE2A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GAGE2A gene location

GeneLoc information about chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP049360:  view genomic region     (about GC identifiers)

Start:
 49,354,118 bp from pter      End:
 49,361,430 bp from pter
Size:
 7,313 bases      Orientation:
 plus strand

Proteins
for GAGE2A gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: GAG2A_HUMAN, Q6NT46 (See protein sequence)
Recommended Name: G antigen 2A  
Size: 116 amino acids; 12785 Da
Miscellaneous: This gene belongs to a multigene family expressed in a large variety of tumors whereas in normal
tissues, expression is restricted to germ cells. These genes organized in clustered repeats, have a high degree of
predicted sequence identity, but differ by scattered single nucleotide substitution. Their sequences contain either
the antigenic peptide YYWPRPRRY or YRPRPRRY which is recognized by cytotoxic T-cells
Caution: The first GAGE nomenclature was based on identified mRNA sequences, but the high identity of the GAGE members
made impossible to separate products of paralogous genes from polymorph products. PubMed:18179644 presented a new GAGE
gene nomenclature based on the identified genes and their products

Explore the universe of human proteins at neXtProt for GAGE2A: NX_Q6NT46

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q6NT46

    • GAGE2A Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_001120684.1  
    ENSEMBL proteins: 
     ENSP00000355421  

    Human Recombinant Protein Products: 
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    Uscn Proteins for GAGE2A

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575cellular_component ND--


    GAGE2A for ontologies           About GeneDecksing



    GAGE2A Antibody Products: 
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    Novus Biologicals GAGE2A Antibody
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    Uscn Antibodies for GAGE2A
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    Assay Products for GAGE2A: 
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    Uscn ELISAs and CLIAs for GAGE2A

    Protein Domains /
    Families
    for GAGE2A gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    GAGE2A for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR008625 GAGE

    Graphical View of Domain Structure for InterPro Entry Q6NT46

    ProtoNet protein and cluster: Q6NT46

    1 Blocks protein family: IPB008625 GAGE

    UniProtKB/Swiss-Prot: GAG2A_HUMAN, Q6NT46
    Similarity: Belongs to the GAGE family


    Function
    for GAGE2A gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    miRNA
    Products:            		Browse 3'-UTR reporter clones for miRNA target validation
    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat GAGE2A
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    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
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    OriGene shRNA RFP: GAGE2A
    OriGene siRNA: GAGE2A
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    Gene Editing
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    Clone
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    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for GAGE2A
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GAGE2A 

    Cell Line
    Products:             		GenScript Custom overexpressing Cell Line Services for GAGE2A
    Search LifeMap BioReagents cell lines for GAGE2A

    In Situ Assay
    Products:       
         		Search Advanced Cell Diagnostics for RNAscope RNA in situ hybridization assays for GAGE2A

    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003674molecular_function ND--


    GAGE2A for ontologies           About GeneDecksing



    Pathways & Interactions
    for GAGE2A gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for GAGE2A

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008150biological_process ND--


    GAGE2A for ontologies           About GeneDecksing



    Drugs & Compounds
    for GAGE2A gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for GAGE2A
    Search CenterWatch for drugs/clinical trials and news about GAGE2A / GAG2A 

    Transcripts
    for GAGE2A gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for GAGE2A gene: 
    NM_001127212.1  

    Unigene Cluster for GAGE2A:

    G antigen 2A
    Hs.722100  [show with all ESTs]
    Unigene Representative Sequence: BG700088
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000362097(uc004doi.4)

    miRNA
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    Inhib. RNA
    Products:             		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for GAGE2A
    OriGene shRNA RFP: GAGE2A
    OriGene siRNA: GAGE2A
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat GAGE2A
    Clone
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    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for GAGE2A
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector: GAGE2A (NM_001127212)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for GAGE2A
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat GAGE2A 
    Primer
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      Search QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat GAGE2A
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat GAGE2A

    Additional cDNA sequence: 

    BC069397.1 CR542236.1 

    1 DOTS entry:

    DT.444944 

    GeneLoc Exon Structure

    5/17 Alternative Splicing Database (ASD) splice patterns (SP) for GAGE2A (see all 17)    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12a · 12b ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^
    SP1:                                                                                                              -     -     -     -     -     -           -   
    SP2:                                                                                                                                                            
    SP3:                                                  -     -     -           -     -     -     -     -     -     -                                             
    SP4:                                                                    -     -     -     -     -                 -     -     -     -     -     -           -   
    SP5:                                                                                                              -           -     -     -     -     -         

    ExUns: 20 ^ 21 ^ 22 ^ 23 ^ 24a · 24b
    SP1:  -     -     -                     
    SP2:                                    
    SP3:                                    
    SP4:  -     -     -                     
    SP5:                                    


    ECgene alternative splicing isoforms for GAGE2A

    Expression
    for GAGE2A gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    GAGE2A expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    GAGE2A expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    TrophoblastMural TrophectodermMural Trophectoderm CellsExtraembryonic Tissues
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See GAGE2A Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for GAGE2A

    SOURCE GeneReport for Unigene cluster: Hs.722100
        SABiosciences Custom PCR Arrays for GAGE2A
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    In Situ
    Assay Products:     
         		Search Advanced Cell Diagnostics for RNAscope RNA in situ hybridization assays for GAGE2A

    Orthologs
    for GAGE2A gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    		   --

    ENSEMBL Gene Tree for GAGE2A (if available)
    TreeFam Gene Tree for GAGE2A (if available) 

    Paralogs
    for GAGE2A gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for GAGE2A gene
    GAGE2C1 2  GAGE2B1 2  GAGE2D1 2  GAGE2E1 2  GAGE12I1 2  GAGE12G1 2  GAGE12F1 2  GAGE131 2  
    GAGE12C1 2  GAGE12D1 2  GAGE12E1 2  GAGE12H1 2  GAGE11 2  GAGE12J1 2  PAGE22  GAGE42  
    XAGE32  GAGE102  PAGE32  XAGE52  XAGE2B2  PAGE52  PAGE2B2  PAGE12  
    XAGE22  PAGE42  
    18/26 SIMAP similar genes for GAGE2A using alignment to 1 protein entry:     GAG2A_HUMAN(see all similar genes):
    GAGE2B    GAGE2C    GAGE2D    GAGE2E    GAGE8    GAGE12F
    GAGE12G    GAGE12I    GAGE7    GAGE12B    GAGE12C    GAGE12D
    GAGE12E    GAGE12H    GAGE12J    GAGE13    GAGE3    GAGE4

    GAGE2A for paralogs           About GeneDecksing



    Genomic Variants
    for GAGE2A gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/47 NCBI SNPs in GAGE2A are shown (see all 47)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr X posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs758189121,2
    		F--49354529(+) GCCCCT/CGGCGG 1 -- int12Minor allele frequency- C:0.37NA 8
    rs778559261,2
    		F--49354544(+) GAGGCG/AGGCGG 1 -- int12Minor allele frequency- A:0.37NA 8
    rs748308981,2
    		F--49354616(+) GAGTGC/TGGAGC 1 -- int12Minor allele frequency- T:0.50NA 8
    rs1430242141,2
    		C--49355179(+) TTATCC/GATGGA 1 -- int10--------
    rs1124256471,2
    		C--49356004(+) GTGTGC/TGTGTT 1 -- int10--------
    rs575143941,2
    		--49356072(+) ATTGAC/GTGGAA 1 -- int10--------
    rs770455041,2
    		C--49356255(+) CATGTA/GTGTTG 1 -- int10--------
    rs786746821,2
    		C--49356308(+) AAAAGA/GCTTTA 1 -- int10--------
    rs766681891,2
    		C--49356310(+) GGCTT-/TACTGT 1 -- int10--------
    rs1469762991,2
    		----49360935(+) AAATGC/TTACAT 1 -- int10--------

    HapMap Linkage Disequilibrium report for GAGE2A (49354118 - 49361430 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 10 variations for GAGE2A
         10 CNVs: 96665 83510 37043 73974 37042 37041 31551 33164 37044 73975

    SABiosciences Cancer Mutation PCR Assays
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    Disorders / Diseases
    for GAGE2A gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    GAGE2A for disorders           About GeneDecksing

    OMIM gene information: 300720    OMIM disorders: --

    2 diseases for GAGE2A:    About MalaCards
    breast cancer    melanoma


    Export disorders for GAGE2A gene to outside databases

    Publications
    for GAGE2A gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for GAGE2A gene integrated from 9 sources:
    (articles sorted by number of sources associating them with GAGE2A)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. The DNA sequence of the human X chromosome. (PubMed id 15772651)1, 2 Ross M.T.... Bentley D.R. (2005)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. A new family of genes coding for an antigen recognized by autologous cytolytic T lymphocytes on a human melanoma. (PubMed id 7544395)1, 3 van den Eynde B....Boon T. (1995)
    4. Combinatorial pharmacologic approaches target EZH2-me diated gene repression in breast cancer cells. (PubMed id 19934278)1 Sun F....Yu Q. (2009)
    5. An overview of the GAGE cancer/testis antigen family with the inclusion of newly identified members. (PubMed id 18179644)2 Gjerstorff M.F. and Ditzel H.J. (2008)
    6. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
    7. Creation of genome-wide protein expression libraries using random activation of gene expression. (PubMed id 11329013)1 Harrington J.J.... Ducar M. (2001)
    8. Characterization of the GAGE genes that are expressed in various human cancers and in normal testis. (PubMed id 10397259)2 De Backer O.... van der Bruggen P. (1999)

    External Searches for GAGE2A gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing GAGE2A gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 729447 HGNC: 4099 Ensembl:ENSG00000189064 euGenes: HUgn729447 ECgene: GAGE2A
    H-InvDB: GAGE2A

    Other Databases showing GAGE2A gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing GAGE2A gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for GAGE2A Pharmacogenomics, SNPs, Pathways

    Intellectual Property
    for GAGE2A gene

    (Patent information from GeneIP,
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