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GADD45GIP1 Gene

protein-coding   GIFtS: 53

GC19M012927
growth arrest and DNA-damage-inducible, gamma interacting protein 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
CKBBP2 1, 2
CRIF1 1, 2, 3
MGC4667 1, 2
MGC4758 1, 2
PLINP-1 1, 2, 3
PLINP1 3
PRG6 1, 2, 3, 5
Plinp1 1, 2
Descriptions
CKII beta binding protein 2 2
CKII beta-associating protein 3
CR6 interacting factor 1 2
CR6-interacting factor 1 3
Papillomavirus L2-interacting nuclear protein 1 3
growth arrest and DNA-damage-inducible, gamma interacting
protein 1 2
p53-responsive gene 6 2
p53-responsive gene 6 protein 3
papillomavirus L2 interacting nuclear protein 1 2
External Ids
HGNC: 299961
Entrez Gene: 904802
UniProtKB: Q8TAE83
Ensembl: ENSG000001792717
Search outside databases for aliases for GADD45GIP1 gene

Previous GC identifer: GC19M012926

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: G45IP_HUMAN, Q8TAE8
Function: Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting
cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in
the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by
inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated
regulation of NR4A1 transcriptional activity

Gene Wiki entry for GADD45GIP1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the GADD45GIP1 gene  

Entrez Gene cytogenetic band: 19p13.13   Ensembl cytogenetic band:  19p13.13   HGNC cytogenetic band: 19p13.2

GADD45GIP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M012927:     (about GC identifiers)

Start:
12,925,972 bp from pter
End:
12,929,050 bp from pter
Size:
3,079 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000019.8  NT_011295.10  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: G45IP_HUMAN, Q8TAE8 (See protein sequence)
Recommended Name: Growth arrest and DNA-damage-inducible proteins-interacting protein 1  
Size: 222 amino acids; 25384 Da
Subunit: Interacts with GADD45A, GADD45B and GADD45G. Interacts with NR4A1 via the NR4A1 AB domain.
Interacts with the human papilloma virus type 16 (HPV 16) minor capsid protein L2
Subcellular location: Nucleus
Miscellaneous: Cells overexpressing GADD45GIP1 were more likely to be in G1 and less likely to be
in S phase and grow more slowly than control cells. Inhibiting the expression of GADD45GIP1
promotes cell cycle progression
Secondary accessions: Q8IVM3 Q8TE51 Q969P9 Q9BSM6

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_443082.2  

    ENSEMBL proteins: 
    ENSP00000323065 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (GADD45GIP1)
    Human Recombinant Proteins from Abnova (GADD45GIP1)
                  OriGene Purified Recombinant Human Protein: GADD45GIP1 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for GADD45GIP1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Cell Signaling Technology (CST) Antibodies for GADD45GIP1  (GADD45 alpha/gamma)
    Antibodies from Abcam (GADD45GIP1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (GADD45GIP1)
    Novus Biologicals Antibody for GADD45GIP1

    Assays for GADD45GIP1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR018472 Damage-induce-interacting_prot


       GeneDecks  GADD45GIP1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8TAE8

    ProtoNet protein and cluster: Q8TAE8

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (GADD45GIP1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_052850

                  Applied Biosystems Silencer® siRNAs for GADD45GIP1

                  Sigma-Aldrich siRNA for GADD45GIP1  
                         Sigma-Aldrich shRNA for GADD45GIP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for GADD45GIP1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_052850
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_052850
                                     untagged cDNA clone in CMV expression vector: NM_052850 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_052850

    UniProtKB/Swiss-Prot: G45IP_HUMAN, Q8TAE8
    Function: Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting
    cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in
    the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by
    inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated
    regulation of NR4A1 transcriptional activity
    Induction: Down-regulated by p53/TP53 in apopoptic cells

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IPI15231747
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/13 Interacting proteins for GADD45GIP1 (ENSP000003230653 Q8TAE81, 2) via UniProtKB, MINT, and/or STRING (see all 13 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    STAT3P407632STRING (score=.951) MINT-6492422 MINT-6492551 MINT-6492512 MINT-6492537
    GADD45BO752932STRING (score=.824) MINT-14285 MINT-14286
    GADD45AP245221, 2STRING (score=.872) EBI-372506, EBI-448167 MINT-16447 MINT-16448 MINT-16446 EBI-372506, EBI-448167 MINT-16447 MINT-16448 MINT-16446
    GADD45GO952571, 2STRING (score=.941) EBI-372506, EBI-448202 MINT-17694 MINT-17693 EBI-372506, EBI-448202 MINT-17694 MINT-17693
    EXOSC4Q9NPD31EBI-372506, EBI-371823
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007049 cell cycle IEA--
    GO:0044419 interspecies interaction between organisms IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for GADD45GIP1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (GADD45GIP1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_052850

                  Sigma-Aldrich siRNA for GADD45GIP1  
                         Sigma-Aldrich shRNA for GADD45GIP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_052850  

    REFSEQ mRNAs for GADD45GIP1 gene: 

    NM_052850.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_052850  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_052850
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_052850
                                     untagged cDNA clone in CMV expression vector: NM_052850 

    Additional cDNA sequence: 

    AF412816.1 AF475095.1 AF479749.1 AJ295986.1 AJ429498.1 BC004944.1 BC013027.2 BC013039.2 
    BC069200.1 

    7 DOTS entries:

    DT.217955  DT.91912547  DT.97759885  DT.121481538  DT.95328208  DT.95328206  DT.121481567 

    24/299 AceView cDNA sequences (see all 299 ):

    AW009704 AF475095 AI185240 BU682200 AI244617 AI356815 AI361663 AI128410 
    AI423524 AI096835 AI300029 BU540995 AW139878 AI334058 AW592583 AJ295986 
    CB048205 BQ083827 AI216175 BQ478251 AI207277 AW134893 AF147080 AI701371 

    highest scoring ESTs for GADD45GIP1:

    AA088470 AA088471 AA149617 AA161035 AA205393 AA286885 AA286886 AA488138 AA502910 AA503634 

    Unigene Cluster for GADD45GIP1:

    Growth arrest and DNA-damage-inducible, gamma interacting protein 1
    Hs.515164  [show with all ESTs]
    Unigene Representative Sequence: BQ052504


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000316939  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    GADD45GIP1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for GADD45GIP1

    1 / 2 / 3

    2 probe-sets matching GADD45GIP1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    212891_s_at2, 3 U133-A 1 1.00 1.00 -- -- BF972185 0.80 1.00 0.91 1

    212891_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GCCAAGATGC

    SOURCE GeneReport for Unigene cluster: Hs.515164

    UniProtKB/Swiss-Prot: G45IP_HUMAN, Q8TAE8
    Tissue specificity: Widely expressed. Highly expressed in the thyroid gland, heart, lymph nodes,
    trachea and adrenal tissues. Expressed at lower level in liver skeletal muscle, kidney, pancreas,
    testis, ovary and stomach. Barely detectable in adrenal adenoma and papillary thyroid cancer

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for GADD45GIP1 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    GADD45GIP11   -- growth arrest and DNA-damage-inducible, gamma interacting more 86.3(n)
    81.28(a)
    476693  XM_533898.2  XP_533898.2 
    chimpanzee
    (Pan troglodytes)
    GADD45GIP11   -- growth arrest and DNA-damage-inducible, gamma interacting more 99.25(n)
    98.2(a)
    747626  XM_001170430.1  XP_001170430.1 
    cow
    (Bos taurus)
    GADD45GIP11   -- growth arrest and DNA-damage-inducible, gamma interacting more 83.78(n)
    81.08(a)
    504718  XM_580882.3  XP_580882.2 
    rat
    (Rattus norvegicus)
    Gadd45gip11   -- growth arrest and DNA-damage-inducible, gamma interacting more 77.18(n)
    75.23(a)
    288916  XM_213842.4  XP_213842.2 
    mouse
    (Mus musculus)
    Gadd45gip11, 5 85
    growth arrest and DNA-damage-inducible, gamma interacting more1, 5 76.28(n)1
    73.42(a)1
    1020601  NM_183358.31  NP_899202.21 
     AI4258835  AK0027385  (see all 12)
    About this table        Species with no ortholog for GADD45GIP1

    ENSEMBL Gene Tree for GADD45GIP1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    8 NCBI SNPs in GADD45GIP1 are shown
    (Click for Applied Biosystems TaqMan ® Genotyping Assay) (only AB snps)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 19 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs29652181,2
    A,C12930398(-) CTATCG/TCAAGG 1 -- ng310--------
    --
    rs104206351,2
    A12931011(+) gggtgC/Tggtgg 1 -- ng310--------
    rs121512061,2
    H12927319(+) TAAACA/CCCAGG 1 -- int14Minor allele frequency- C:0.00EU EA WA 418
    --
    rs717946321,2
    --12928721(+) CGCAG-/CGACTCC 1 S/ fra10--------
    --
    rs115389961,2
    --12926162(-) TCCAGG/TAGCTG 1 E/X non1 ese30--------
    --
    rs559814161,2
    --12930512(+) TATACA/TTTTAA 1 -- ng310--------
    --
    rs621098721,2
    --12928125(+) CCACCA/GCCCCC 1 -- int10--------
    --
    rs579385521,2
    --12926531(+) ATGAAA/TTTGAT 1 -- int10--------
    About this table
    The following additional 28 SNP(s) have been found in Applied Biosystems data source:
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)


     rs8111548 rs10424909 rs2242518 rs8109230 rs4987202 rs8240 rs8108349
     rs1059298 rs2974755 rs2974752 rs1063415 rs1803719 rs45604337 rs1803718
     rs11883418 rs11667602 rs1801594 rs746024 rs11558955 rs3865487 rs7395
     rs1059251 rs15340 rs3208320 rs4987203 rs4944 rs2227995 rs3179165

    HapMap Linkage Disequilibrium images for GADD45GIP1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 605162

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/12 PubMed articles for GADD45GIP1 gene (see all 12 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 90480 HGNC: 29996 AceView: GADD45GIP1 Ensembl:ENSG00000179271 euGenes: HUgn90480
    ECgene: GADD45GIP1 H-InvDB: GADD45GIP1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for GADD45GIP1 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for GADD45GIP1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for GADD45GIP1 Browse small molecules at Sigma-Aldrich
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     Search Tocris compounds for GADD45GIP1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for GADD45GIP1

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