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GADD45B Gene

protein-coding   GIFtS: 60

GC19P002427
growth arrest and DNA-damage-inducible, beta
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: MYD118)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
DKFZP566B133 1
DKFZp566B133 2
GADD45BETA 1, 2
MYD118 2, 3, 5
Descriptions
Myeloid differentiation primary response protein MyD118 3
Negative growth regulatory protein MyD118 3
growth arrest and DNA-damage-inducible, beta 2
myeloid differentiation primary response 2
External Ids
HGNC: 40961
Entrez Gene: 46162
UniProtKB: O752933
Ensembl: ENSG000000998607
Search outside databases for aliases for GADD45B gene

Previous GC identifers: GC19P002538 GC19P002416

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for GADD45B:
This gene is a member of a group of genes whose transcript levels are increased following stressful
growth arrest conditions and treatment with DNA-damaging agents. The genes in this group respond
to environmental stresses by mediating activation of the p38/JNK pathway. This activation is
mediated via their proteins binding and activating MTK1/MEKK4 kinase, which is an upstream
activator of both p38 and JNK MAPKs. The function of these genes or their protein products is
involved in the regulation of growth and apoptosis. These genes are regulated by different
mechanisms, but they are often coordinately expressed and can function cooperatively in inhibiting
cell growth. [provided by RefSeq]

UniProtKB/Swiss-Prot: GA45B_HUMAN, O75293
Function: Involved in the regulation of growth and apoptosis. Mediates activation of
stress-responsive MTK1/MEKK4 MAPKKK

Gene Wiki entry for GADD45B

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the GADD45B gene  

Entrez Gene cytogenetic band: 19p13.3   Ensembl cytogenetic band:  19p13.3   HGNC cytogenetic band: 19p13.3

GADD45B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P002427:     (about GC identifiers)

Start:
2,427,135 bp from pter
End:
2,429,257 bp from pter
Size:
2,123 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000019.8  NT_011255.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: GA45B_HUMAN, O75293 (See protein sequence)
Recommended Name: Growth arrest and DNA-damage-inducible protein GADD45 beta  
Size: 160 amino acids; 17818 Da
Subunit: Interacts with GADD45GIP1
Secondary accessions: O75960 Q17R46

REFSEQ proteins: NP_056490.2  

ENSEMBL proteins: 
ENSP00000215631 


Human Recombinant Proteins 
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Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (GADD45B)
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                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


Antibodies for GADD45B: 
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Sigma-Aldrich Antibodies for GADD45B
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Antibodies from Abcam (GADD45B), each with their AbpromiseSM
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Search Novus for antibodies for GADD45B

Assays for GADD45B: 
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Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR004038 Ribosomal_L7Ae/L30e/S12e/Gad45


   GeneDecks  GADD45B for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry O75293

ProtoNet protein and cluster: O75293

1 Blocks protein family: IPB004038 Ribosomal protein L7Ae/L30e/S12e/Gadd45

UniProtKB/Swiss-Prot: GA45B_HUMAN, O75293
Similarity: Belongs to the GADD45 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (GADD45B)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015675

              Applied Biosystems Silencer® siRNAs for GADD45B

              Sigma-Aldrich siRNA for GADD45B  
                     Sigma-Aldrich shRNA Panels and shRNA for GADD45B  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_015675
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_015675
                                 untagged cDNA clone in CMV expression vector: NM_015675 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_015675

UniProtKB/Swiss-Prot: GA45B_HUMAN, O75293
Function: Involved in the regulation of growth and apoptosis. Mediates activation of
stress-responsive MTK1/MEKK4 MAPKKK

Genatlas biochemistry entry for GADD45B:
growth arrest and DNA-damage-inducible,beta,ubiquitously expressed,activating MEKK4

4 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Gadd45b):

cellularhematopoietic systemimmune systemrenal/urinary system

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005515 protein binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


3 Millipore Pathways for GADD45B
 DNA damage ATM/ATR regulation of G1/S checkpoint
 Immune response IL-12-induced IFN-gamma production
 Development TGF-beta receptor signaling

   GeneDecks  GADD45B for the pathways selected above  
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  GADD45B  (Your Favorite Gene powered by Ingenuity) 
 p53 Signaling
 ATM Signaling

   GeneDecks  GADD45B for the pathways selected above  
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3 Kegg Pathways  (Kegg details for GADD45B):
 hsa04010 MAPK signaling pathway
 hsa04110 Cell cycle
 hsa04115 p53 signaling pathway

   GeneDecks  GADD45B for the pathways selected above  
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 Gene Network CentralTM Interacting Genes and Proteins Network for  GADD45B 


5/38 Interacting proteins for GADD45B (O752932 ENSP000002156313) via UniProtKB, MINT, and/or STRING (see all 38 )
InteractantInteraction Details
GeneCardExternal ID(s)
GADD45GIP1Q8TAE82, ENSP000003230653MINT-14285 MINT-14286 STRING (score=.824)
PCNAENSP000003684383STRING (score=.999)
CDC2ENSP000003629173STRING (score=.996)
RELAENSP000003115083STRING (score=.972)
GADD45AENSP000003600253STRING (score=.961)
About this table

5/8 Gene Ontology (GO) biological process terms (links to tree view) (see all 8 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0000185 activation of MAPKKK activity TAS9827804
GO:0000186 activation of MAPKK activity IEA--
GO:0006469 negative regulation of protein kinase activity IEA--
GO:0006915 apoptosis TAS9827804
GO:0006950 response to stress TAS9827804
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for GADD45B

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (GADD45B)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015675

              Sigma-Aldrich siRNA for GADD45B  
                     Sigma-Aldrich shRNA Panels and shRNA for GADD45B  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_015675  

REFSEQ mRNAs for GADD45B gene: 

NM_015675.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_015675  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_015675
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_015675
                                 untagged cDNA clone in CMV expression vector: NM_015675 

Additional cDNA sequence: 

AF078077.1 AF087853.1 AK293087.1 AL050044.1 AY615270.1 AY615271.1 BC024654.3 BC113466.1 
BC117469.1 

10 DOTS entries:

DT.102827445  DT.97855933  DT.95267656  DT.121475454  DT.91924709  DT.97769928  DT.97855934  DT.121475448 
DT.121475457  DT.40306306 

24/603 AceView cDNA sequences (see all 603 ):

AA429118 BM708634 BM991001 BM748511 BQ723443 BM757579 BM748552 BM749738 
BM750581 CB131148 BM692252 BM748565 BM829409 AA806506 BM758264 BM758994 
BM757977 BU168386 BM670459 BM749736 BM757667 AA402108 CA306845 BM787036 

highest scoring ESTs for GADD45B:

AF078077 AA004360 AA018764 AA039363 AA039364 AA056235 AA069206 AA113188 AA194909 AA203558 

Unigene Cluster for GADD45B:

Growth arrest and DNA-damage-inducible, beta
Hs.110571  [show with all ESTs]
Unigene Representative Sequence: AF087853


GeneLoc Exon Structure

5/7 Alternative Splicing Database (ASD) splice patterns (SP) for GADD45B (see all 7 )

ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3a · 3b · 3c ^ 4a · 4b · 4c · 4d
SP1:              -     -           -                 -                           
SP2:              -     -           -                 -                           
SP3:                    -           -                                             
SP4:              -     -     -     -                 -                           
SP5:              -     -           -                                             

About this scheme

ECgene alternative splicing isoforms for GADD45B

1 Ensembl transcript including schematic representation:
ENST00000215631  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

GADD45B expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for GADD45B

1 / 2 / 3

7 probe-sets matching GADD45B gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

39822_s_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AF078077 1.00 1.00 1.00 1

209304_x_at2, 3 U133-A 1 1.00 1.00 -- -- AF087853 0.80 1.00 0.91 1

207574_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_015675 0.40 1.00 0.76 1

209305_s_at2, 3 U133-A 1 1.00 1.00 -- -- AF078077 1.00 1.00 1.00 1

209304_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

207574_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

209305_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AACTCCCAGT

SOURCE GeneReport for Unigene cluster: Hs.110571

Expression variation in blood from EXPOLDB for GADD45B

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for GADD45B gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
GADD45B1   -- growth arrest and DNA-damage-inducible, beta 92.5(n)
98.12(a)
485069  XM_542187.2  XP_542187.1 
chimpanzee
(Pan troglodytes)
GADD45B1   -- growth arrest and DNA-damage-inducible, beta 99.58(n)
100(a)
744311  XM_001151983.1  XP_001151983.1 
cow
(Bos taurus)
GADD45B1   -- growth arrest and DNA-damage-inducible, beta 91.25(n)
96.87(a)
618405  NM_001040604.1  NP_001035694.1 
rat
(Rattus norvegicus)
Gadd45b1   -- growth arrest and DNA-damage-inducible, beta 85.42(n)
92.5(a)
299626  NM_001008321.1  NP_001008322.1 
mouse
(Mus musculus)
Gadd45b1, 5 10 (60.50 cM)5
growth arrest and DNA-damage-inducible 45 beta1, 5 86.25(n)1
93.12(a)1
178731  NM_008655.11  NP_032681.11 
 AF1760455  AF4418605  (see all 12)
About this table        Species with no ortholog for GADD45B

ENSEMBL Gene Tree for GADD45B
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for GADD45B gene
GADD45G2  GADD45A2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10 NCBI SNPs in GADD45B are shown
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (only AB snps)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 19 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs37834901,2
C,F,H2426035(+) ACTAGC/GCTATG 1 -- ng516Minor allele frequency- G:0.01NS EU EA WA NA 680
rs37834891,2
F,H2425588(+) GTAAAA/CCATTG 1 -- ng515Minor allele frequency- C:0.01NS EU EA WA 586
--
rs48072801,2
A,C2425171(+) ccacgA/Cccagc 1 -- ng510--------
rs37950241,2
F,H2426411(+) GGAGGC/TGCCCA 1 -- ng514Minor allele frequency- T:0.06EU EA WA 382
rs37834921,2
C,F2426289(+) TCTCCC/AGGGCC 1 -- ng517Minor allele frequency- A:0.02NS 718
rs96768931,2
H2426060(+) CAGTTT/CCCCCT 1 -- ng51 trp34Minor allele frequency- C:0.00EU EA WA 418
rs37834911,2
H2426121(+) ATCATC/GCATGA 1 -- ng51 tfbs35Minor allele frequency- G:0.00NS EU EA WA 588
--
rs559371481,2
--2425142(+) GAGTA-/AGCTGG 1 -- ng510--------
--
rs37834881,2
--2425452(+) gtctcG/Caactc 1 -- ng511Minor allele frequency- C:0.01NS 172
--
rs37834871,2
--2425385(+) caCTTA/GTCCCA 1 -- ng511Minor allele frequency- G:0.01NS 174
About this table
The following additional 31 SNP(s) have been found in Applied Biosystems data source:
(Click for Applied Biosystems TaqMan ® Genotyping Assay)


 rs929461 rs4807279 rs3783494 rs3783506 rs12982381 rs4473311 rs11670333
 rs7253793 rs15306 rs3783501 rs3783510 rs2302181 rs2024144 rs3783508
 rs1548710 rs3729535 rs28460270 rs1548709 rs4806857 rs8109281 rs13345846
 rs7249224 rs3783496 rs3783498 rs3729534 rs11673422 rs10418392 rs16991159
 rs4807273 rs10420027 rs1548708

HapMap Linkage Disequilibrium images for GADD45B (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 604948

2 Novoseek disease relationships for GADD45B gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
lung carcinoma 46.31 3 10101024 (1), 10490824 (1), 11251170 (1)
leukemia 14.87 1 11251170 (1)
About this table

Human Genome Epidemiology Navigator: GADD45B (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/52 PubMed articles for GADD45B gene (see all 52 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 4616 HGNC: 4096 AceView: GADD45B Ensembl:ENSG00000099860 euGenes: HUgn4616
ECgene: GADD45B H-InvDB: GADD45B
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for GADD45B Genetics and Cytogenetics in Oncology and Haematology
NIEHS-SNPshttp://egp.gs.washington.edu/data/gadd45b/
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for GADD45B:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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