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GABARAPL2 Gene

protein-coding   GIFtS: 60

GC16P074157
GABA(A) receptor-associated protein-like 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
ATG8 1, 2
FLC3A 3
GATE-16 1, 2, 3
GATE16 1, 2
GEF-2 3
GEF2 1, 2, 3
Descriptions
GABA(A) receptor-associated protein-like 2 2, 3
Ganglioside expression factor 2 2, 3
General protein transport factor p16 3
Golgi-associated ATPase enhancer of 16 kDa 3
MAP1 light chain 3 related protein 2
MAP1 light chain 3-related protein 3
External Ids
HGNC: 132911
Entrez Gene: 113452
UniProtKB: P605203
Ensembl: ENSG000000347137
Search outside databases for aliases for GABARAPL2 gene

Previous GC identifers: GC16P066530 GC16P076560 GC16P075339 GC16P075380 GC16P075381

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: GBRL2_HUMAN, P60520
Function: Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between
NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn
stimulates the association with GOSR1 (By similarity)

Gene Wiki entry for GABARAPL2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the GABARAPL2 gene  

Entrez Gene cytogenetic band: 16q22.3-q24.1   Ensembl cytogenetic band:  16q23.1   HGNC cytogenetic band: 16q22.1

GABARAPL2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16P074157:     (about GC identifiers)

Start:
74,157,750 bp from pter
End:
74,169,280 bp from pter
Size:
11,531 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000016.8  NT_010498.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: GBRL2_HUMAN, P60520 (See protein sequence)
Recommended Name: Gamma-aminobutyric acid receptor-associated protein-like 2  
Size: 117 amino acids; 13667 Da
Subunit: Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin (By similarity). Interacts
with ULK1
Subcellular location: Golgi apparatus (By similarity)
Secondary accessions: O08765 Q9DCP8 Q9UQF7

Post-translational modifications:

  • Phosphorylated upon DNA damage, probably by ATM or ATR1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_009216.1  

    ENSEMBL proteins: 
    ENSP00000037243 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (GABARAPL2)
    Human Recombinant Proteins from Abnova (GABARAPL2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139 Golgi membrane ISS10747018 11414770
    GO:0000421 autophagic vacuole membrane IDA15169837
    GO:0005622 intracellular IDA11256614
    GO:0005624 membrane fraction IDA12507496
    GO:0005794 Golgi apparatus ISS11414770
    About this table

    Antibodies for GABARAPL2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for GABARAPL2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (GABARAPL2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (GABARAPL2)
    Novus Biologicals Antibodies for GABARAPL2

    Assays for GABARAPL2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR004241 MAP1_LC3


       GeneDecks  GABARAPL2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P60520

    ProtoNet protein and cluster: P60520

    1 Blocks protein family: IPB004241 Light chain 3 (LC3)

    UniProtKB/Swiss-Prot: GBRL2_HUMAN, P60520
    Similarity: Belongs to the MAP1 LC3 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (GABARAPL2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (GABARAPL2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_007285

                  Applied Biosystems Silencer® siRNAs for GABARAPL2

                  Sigma-Aldrich siRNA for GABARAPL2  
                         Sigma-Aldrich shRNA for GABARAPL2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for GABARAPL2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_007285
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_007285
                                     untagged cDNA clone in CMV expression vector: NM_007285 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_007285

    UniProtKB/Swiss-Prot: GBRL2_HUMAN, P60520
    Function: Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between
    NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn
    stimulates the association with GOSR1 (By similarity)

    5 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000149 SNARE binding ISS10747018
    GO:0008017 microtubule binding NAS11414770
    GO:0048487 beta-tubulin binding ISS--
    GO:0050811 GABA receptor binding NAS11414770
    GO:0051117 ATPase binding ISS10747018
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Kegg Pathway  (Kegg details for GABARAPL2):
     hsa04140 Regulation of autophagy

       GeneDecks  GABARAPL2 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  GABARAPL2 


    5/24 Interacting proteins for GABARAPL2 (ENSP000000372433 P605201, 2) via UniProtKB, MINT, and/or STRING (see all 24 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ATG3Q9NT621STRING (score=.976) EBI-720116, EBI-988094
    ATG4BQ9Y4P11, 2STRING (score=.977) EBI-720116, EBI-712014 MINT-63922 EBI-720116, EBI-712014 MINT-63922
    EIF4G1Q046371EBI-720116, EBI-73711
    EZRP153111EBI-720116, EBI-1056902
    HBG1P698911EBI-720116, EBI-1056789
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006891 intra-Golgi vesicle-mediated transport ISS10747018
    GO:0006914 autophagy NAS12507496
    GO:0015031 protein transport IEA--
    GO:0032781 positive regulation of ATPase activity ISS10747018
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for GABARAPL2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (GABARAPL2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (GABARAPL2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_007285

                  Sigma-Aldrich siRNA for GABARAPL2  
                         Sigma-Aldrich shRNA for GABARAPL2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_007285  

    REFSEQ mRNAs for GABARAPL2 gene: 

    NM_007285.6   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_007285  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_007285
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_007285
                                     untagged cDNA clone in CMV expression vector: NM_007285 

    Additional cDNA sequence: 

    AB030710.1 AF077046.1 AF087848.1 AJ010569.1 AK289788.1 AK311266.1 BC005985.1 BC014594.2 
    BC029601.1 BC040312.1 CR542217.1 CR592344.1 CR603478.1 CR621062.1 CR624907.1 

    14 DOTS entries:

    DT.117538  DT.100791078  DT.100657358  DT.91764685  DT.120669693  DT.95161731  DT.100791075  DT.95161726 
    DT.100684495  DT.120669675  DT.120669800  DT.91764676  DT.91764672  DT.95161738 

    24/369 AceView cDNA sequences (see all 369 ):

    CR603478 NM_007285 AA127496 CF456408 CA395633 CA389312 BM709773 BU661144 
    AI363487 AW571871 N66389 CA941995 AA152046 CR621062 BQ004090 AA149807 
    BM874519 AA400231 BU658824 AI702598 BQ774307 CR624907 AW264252 BU163903 

    highest scoring ESTs for GABARAPL2:

    AA063027 AA099173 AA101923 AA127496 AA127591 AA149807 AA149872 AA224361 AA282403 AA364905 

    Unigene Cluster for GABARAPL2:

    GABA(A) receptor-associated protein-like 2
    Hs.461379  [show with all ESTs]
    Unigene Representative Sequence: BC040312


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for GABARAPL2

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b
    SP1:              -                 -               
    SP2:                                -               
    SP3:              -           -     -               
    SP4:              -                                 

    About this scheme

    ECgene alternative splicing isoforms for GABARAPL2

    1 Ensembl transcript including schematic representation:
    ENST00000037243  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    GABARAPL2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for GABARAPL2

    1 / 2 / 3

    3 probe-sets matching GABARAPL2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    35767_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AI565760 0.80 1.00 0.91 1

    209046_s_at2, 3 U133-A 1 1.00 1.00 -- -- AB030710 0.80 1.00 0.91 1

    209046_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TTGGACTGAG

    SOURCE GeneReport for Unigene cluster: Hs.461379

    Expression variation in blood from EXPOLDB for GABARAPL2

    UniProtKB/Swiss-Prot: GBRL2_HUMAN, P60520
    Tissue specificity: Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary,
    spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for GABARAPL2 gene from 5/16 species (see all 16 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    GABARAPL21   -- GABA(A) receptor-associated protein-like 2 96.3(n)
    100(a)
    479646  XM_536778.2  XP_536778.2 
    chimpanzee
    (Pan troglodytes)
    GABARAPL21   -- GABA(A) receptor-associated protein-like 2 99.72(n)
    100(a)
    454251  XM_511114.2  XP_511114.2 
    cow
    (Bos taurus)
    GABARAPL21   -- GABA(A) receptor-associated protein-like 2 95.44(n)
    100(a)
    282531  NM_174675.2  NP_777100.1 
    rat
    (Rattus norvegicus)
    Gabarapl21   -- GABA(A) receptor-associated protein like 2 94.3(n)
    100(a)
    64670  NM_022706.2  NP_073197.1 
    mouse
    (Mus musculus)
    Gabarapl25
    1000422111
    85
    gamma-aminobutyric acid (GABA) A receptor-associated more5
    predicted gene, 1000422111
    92.88(n)1
    99.15(a)1
    1000422111  XM_001478223.11  XP_001478273.11 
     AA1243245  AF1906445  (see all 18)
    About this table        Species with no ortholog for GABARAPL2

    ENSEMBL Gene Tree for GABARAPL2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for GABARAPL2 gene
    MAP1LC3B2  MAP1LC3A2  MAP1LC3C2  MAP1LC3B22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/63 NCBI SNPs in GABARAPL2 are shown (see all 63 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 30)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 16 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs118658741,2
    C,F74156437(+) GGTGCA/GTCAGA 1 -- ng514Minor allele frequency- G:0.03NA 232
    --
    rs115562971,2
    C,H74159556(+) AGCTTC/ACTTCT 1 T/P mis18Minor allele frequency- A:0.00EU EA WA 804
    --
    rs27921,2
    F74169137(-) TGGATA/GATTTT 1 -- ut311Minor allele frequency- G:0.01MN 184
    --
    rs81925191,2
    H74168989(+) TGCCTG/ATATTC 1 -- ut314Minor allele frequency- A:0.00EU EA WA 420
    rs10435431,2
    C74157878(+) TCCCGC/TCGCCG 1 -- ut510--------
    --
    rs115562911,2
    H74159497(+) CTTGGT/CTCCAT 1 A/V mis14Minor allele frequency- C:0.00EU EA WA 400
    rs65642671,2
    A,C,F,H74161426(+) TCAGCG/TCTTTG 1 -- int18Minor allele frequency- N:0.00EA NA EU WA 626
    rs111498411,2
    A,C,F,H74160298(+) TGGATG/TGGCTC 1 -- int116Minor allele frequency- T:0.10EA NA EU WA 1470
    rs80609721,2
    A,C,F,H74164530(+) GTTTAA/TCTTCT 1 -- int14Minor allele frequency- T:0.48EU EA WA 412
    rs125980941,2
    C,F,H74166087(+) TAAAGA/GTGACC 1 -- int14Minor allele frequency- G:0.36EU EA WA 410
    About this table

    HapMap Linkage Disequilibrium images for GABARAPL2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607452

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/22 PubMed articles for GABARAPL2 gene (see all 22 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 11345 HGNC: 13291 AceView: GABARAPL2 Ensembl:ENSG00000034713 euGenes: HUgn11345
    ECgene: GABARAPL2 H-InvDB: GABARAPL2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for GABARAPL2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for GABARAPL2 Antibodies for GABARAPL2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
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