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G3BP2 Gene

protein-coding   GIFtS: 51

GC04M076758
GTPase activating protein (SH3 domain) binding protein 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
G3BP-2 3
KIAA0660 1, 3
Descriptions
GAP SH3 domain-binding protein 2 3
GTPase activating protein (SH3 domain) binding protein 2 2
Ras-GTPase activating protein SH3 domain-binding protein 2 2
External Ids
HGNC: 302911
Entrez Gene: 99082
UniProtKB: Q9UN863
Ensembl: ENSG000001387577
Search outside databases for aliases for G3BP2 gene

Previous GC identifers: GC04M076463 GC04M076960 GC04M077026 GC04M076925

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: G3BP2_HUMAN, Q9UN86
Function: Probable scaffold protein that may be involved in mRNA transport (Potential)

Gene Wiki entry for G3BP2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the G3BP2 gene  

Entrez Gene cytogenetic band: 4q21.1   Ensembl cytogenetic band:  4q21.1   HGNC cytogenetic band: 4q21.1

G3BP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04M076758:     (about GC identifiers)

Start:
76,786,990 bp from pter
End:
76,817,629 bp from pter
Size:
30,640 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000004.10  NT_016354.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: G3BP2_HUMAN, Q9UN86 (See protein sequence)
Recommended Name: Ras GTPase-activating protein-binding protein 2  
Size: 482 amino acids; 54121 Da
Subunit: Binds to the N-terminal domain of I-kappa-B-alpha
Subcellular location: Cytoplasm
Secondary accessions: O60606 O75149 Q9UPA1
Alternative splicing: 2 isoforms:  Q9UN86-1   Q9UN86-2   

Post-translational modifications:

  • Arg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (3 alternative transcripts): 
    NP_036429.2  NP_987100.1  NP_987101.1  


    ENSEMBL proteins: 
    ENSP00000352738 ENSP00000350518 ENSP00000379069 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (G3BP2)
                  OriGene Purified Recombinant Human Protein: G3BP2 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622 intracellular IEA--
    GO:0005737 cytoplasm NAS10969074
    About this table

    Antibodies for G3BP2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for G3BP2
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for G3BP2

    Assays for G3BP2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR002075 NTF2
     IPR018222 Nuclear_transport_factor_2_euk
     IPR000504 RRM_RNP1
     IPR012677 a_b_plait_nuc_bd


       GeneDecks  G3BP2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UN86

    ProtoNet protein and cluster: Q9UN86

    2 Blocks protein families:
    IPB000504 RNA-binding region RNP-1 (RNA recognition motif)
    IPB002075 Nuclear transport factor 2 (NTF2)


    UniProtKB/Swiss-Prot: G3BP2_HUMAN, Q9UN86
    Similarity: Contains 1 NTF2 domain
    Similarity: Contains 1 RRM (RNA recognition motif) domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (G3BP2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (G3BP2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_203504

                  Applied Biosystems Silencer® siRNAs for G3BP2

                  Sigma-Aldrich siRNA for G3BP2  
                         Sigma-Aldrich shRNA for G3BP2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for G3BP2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_012297
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_012297
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_012297 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_012297

    UniProtKB/Swiss-Prot: G3BP2_HUMAN, Q9UN86
    Function: Probable scaffold protein that may be involved in mRNA transport (Potential)

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003723 RNA binding NAS--
    GO:0005515 protein binding IPI17353931 17474147
    GO:0030159 receptor signaling complex scaffold activity NAS10969074
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  G3BP2 


    5/67 Interacting proteins for G3BP2 (Q9UN861 ENSP000003527383) via UniProtKB, MINT, and/or STRING (see all 67 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ABL1P005191EBI-1044298, EBI-375543
    STK24Q5T5B31EBI-1044298, EBI-1054809
    ABRENSP000003039093STRING (score=.899)
    AKAP13ENSP000003547183STRING (score=.899)
    ARHGAP4ENSP000003590453STRING (score=.899)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006810 transport IEA--
    GO:0007253 cytoplasmic sequestering of NF-kappaB NAS10969074
    GO:0007265 Ras protein signal transduction NAS9575347
    GO:0051028 mRNA transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for G3BP2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (G3BP2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (G3BP2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_203504

                  Sigma-Aldrich siRNA for G3BP2  
                         Sigma-Aldrich shRNA for G3BP2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_012297  NM_203504  NM_203505  

    REFSEQ mRNAs for G3BP2 gene (3 alternative transcripts): 

    NM_012297.4   NM_203504.2   NM_203505.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_012297  NM_203504  NM_203505  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_012297
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_012297
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_012297 

    Additional cDNA sequence: 

    AB014560.1 AF051311.1 AF053535.1 AF070615.1 AF145284.1 AK291772.1 AK291786.1 BC011731.2 

    24/38 DOTS entries (see all 38 ):

    DT.95133911  DT.97785956  DT.454665  DT.100828171  DT.121228664  DT.100722540  DT.121228537  DT.121228679 
    DT.91825578  DT.102843995  DT.100828168  DT.121228625  DT.100799988  DT.100799597  DT.121228550  DT.91699073 
    DT.100828176  DT.121228545  DT.121228546  DT.40119218  DT.99961712  DT.100828169  DT.100828174  DT.121228548 

    24/451 AceView cDNA sequences (see all 451 ):

    AA905408 AI564324 BQ921099 F11785 AA577211 BQ710582 BE676426 BU161990 
    BU155808 BQ061154 BQ880868 BQ222199 BQ049961 BM478534 CA429088 AA781267 
    T30867 BU192740 NM_012297 AI581388 BM456585 BF817108 AI863267 CA442944 

    highest scoring ESTs for G3BP2:

    AF053535 AF145284 AL537462 AL710629 AL710930 AU077111 AU280134 BC011731 BF815661 BF975851 

    Unigene Cluster for G3BP2:

    GTPase activating protein (SH3 domain) binding protein 2
    Hs.303676  [show with all ESTs]
    Unigene Representative Sequence: NM_203505


    GeneLoc Exon Structure

    3 Ensembl transcripts including schematic representations:
    ENST00000359707  ENST00000357854  ENST00000395719  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    G3BP2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for G3BP2

    1 / 2 / 3

    8 probe-sets matching G3BP2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    35793_at2, 3 U95-A 1 1.00 1.00 0.87 1.24 AB014560 0.80 0.80 0.80 1
    67246_at2, 3 U95-E 1 0.50 1.00 0.96 0.76 AI040381 0.60 1.00 0.82 1

    206383_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_012297 0.60 1.00 0.82 1

    208840_s_at2, 3 U133-A 1 1.00 1.00 -- -- AU149503 0.40 1.00 0.76 1

    208841_s_at2, 3 U133-A 1 1.00 1.00 -- -- AB014560 0.80 0.80 0.80 1

    206383_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    208840_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    208841_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  G3BP2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GTGATTGTTC

    SOURCE GeneReport for Unigene cluster: Hs.303676

    Expression variation in blood from EXPOLDB for G3BP2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for G3BP2 gene from 5/11 species (see all 11 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    G3BP21   -- GTPase activating protein (SH3 domain) binding protein more 95.71(n)
    98.34(a)
    478429  XM_851100.1  XP_856193.1 
    chimpanzee
    (Pan troglodytes)
    G3BP21   -- GTPase activating protein (SH3 domain) binding protein more 99.79(n)
    100(a)
    461244  XM_517219.2  XP_517219.2 
    cow
    (Bos taurus)
    G3BP21   -- GTPase activating protein (SH3 domain) binding protein more 94.88(n)
    98.44(a)
    539249  NM_001046455.1  NP_001039920.1 
    rat
    (Rattus norvegicus)
    G3bp21   -- GTPase activating protein (SH3 domain) binding protein more 91.69(n)
    97.77(a)
    305240  NM_001013989.1  NP_001014011.1 
    mouse
    (Mus musculus)
    G3bp21, 5 55
    GTPase activating protein (SH3 domain) binding protein more1, 5 93.22(n)1
    98.34(a)1
    238811  NM_011816.31  NP_035946.21 
     AA4095415  AF1452855  (see all 42)
    About this table        Species with no ortholog for G3BP2

    ENSEMBL Gene Tree for G3BP2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for G3BP2 gene
    G3BP12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/204 NCBI SNPs in G3BP2 are shown (see all 204 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 49)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 4 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs18018381,2
    C,F,H76787633(-) CAGAAA/GATTAC 3 -- ut314Minor allele frequency- G:0.04EU EA WA 354
    rs115561461,2
    C,F,H76788577(-) ATTTAT/CCTTGT 3 -- ut316Minor allele frequency- C:0.13NA EA 376
    --
    rs110970441,2
    A,C76787505(-) atataA/Taattt 3 -- ut31 ese30--------
    --
    rs413983501,2
    F76818463(-) CTTCAT/CTCCAA 3 -- ng314Minor allele frequency- C:0.15EU EA WA 418
    --
    rs172154001,2
    F76787809(+) GAAAAA/CGGGAC 3 -- ut31 trp34Minor allele frequency- C:0.13NA EA 202
    rs93069941,2
    A,C,F,H76795140(+) GTTCAG/ATAACT 3 -- int113Minor allele frequency- A:0.44NS EU EA WA 934
    rs21374291,2
    A,C,F,H76810111(+) TAGTGA/CCAGAA 3 -- int18Minor allele frequency- N:0.00EU EA WA NA 630
    --
    rs110970511,2
    A,C,F,H76796884(+) ATGTCC/TCACTG 3 -- int14Minor allele frequency- T:0.45EU EA WA 406
    rs65318161,2
    A,C,F,H76800799(+) AAAAAA/CAGGAA 3 -- int1 trp316Minor allele frequency- N:0.00EA NA EU WA 1474
    rs68169781,2
    A,C,F,H76793427(+) CATAAC/TCTTAA 3 -- int18Minor allele frequency- T:0.31EU EA WA 832
    About this table

    HapMap Linkage Disequilibrium images for G3BP2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/20 PubMed articles for G3BP2 gene (see all 20 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 9908 HGNC: 30291 AceView: G3BP2 Ensembl:ENSG00000138757 euGenes: HUgn9908
    ECgene: G3BP2 H-InvDB: G3BP2
    (According to HUGE)
    About This Section

    HUGE: KIAA0660
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for G3BP2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for G3BP2 Antibodies for G3BP2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
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