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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

FHL5 Gene

protein-coding   GIFtS: 54
GCID: GC06P097056

four and a half LIM domains 5

 Explore 9 diseases affiliated with
FHL5 via our new
 Human Malady Compendium 
Biological research products
for FHL5
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Four And A Half LIM Domains 51 2     FLJ330491
ACT1 2 3 5     1700027G07Rik2
DJ393D12.21     RP3-393D12.22
Activator Of CAMP-Responsive Element Modulator In Testis2 3     Activator Of CAMP-Responsive Element Modulator (CREM) In Testis2
Activator Of CREM In Testis2 3     Four And A Half LIM Domains Protein 52
FHL-52 3     LIM Protein ACT2

External Ids:    HGNC: 173711   Entrez Gene: 94572   Ensembl: ENSG000001122147   OMIM: 6051265   UniProtKB: Q5TD973   

Export aliases for FHL5 gene to outside databases

Previous GC identifers: GC06P097117 GC06P094444


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for FHL5:
The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM).
It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription
factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation
sites found in this gene. Alternative splicing results in multiple transcript variants encoding the same protein.
(provided by RefSeq, Dec 2009)

UniProtKB/Swiss-Prot: FHL5_HUMAN, Q5TD97
Function: May be involved in the regulation of spermatogenesis. Stimulates CREM transcriptional activity in a
phosphorylation-independent manner

Gene Wiki entry for FHL5


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000006.11  NC_018917.1  NT_025741.15  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the FHL5 gene promoter:
         STAT5B   STAT5A   Nkx2-5   LCR-F1   Evi-1   RORalpha1   Pax-3   IRF-7A   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidFHL5 promoter sequence
   Search SABiosciences Chromatin IP Primers for FHL5

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat FHL5


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 6q16.1-q16.3   Ensembl cytogenetic band:  6q16.1   HGNC cytogenetic band: 6q16.1-q16.3

FHL5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
FHL5 gene location

GeneLoc information about chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06P097056:  view genomic region     (about GC identifiers)

Start:
97,010,424 bp from pter      End:
97,064,512 bp from pter
Size:
54,089 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: FHL5_HUMAN, Q5TD97 (See protein sequence)
Recommended Name: Four and a half LIM domains protein 5  
Size: 284 amino acids; 32720 Da
Subunit: Interacts with CREM via the third LIM domain (By similarity)
Subcellular location: Nucleus. Note=Nuclei of round and elongated spermatids
1 PDB 3D structure from and Proteopedia for FHL5:
1X68 (3D)    
Secondary accessions: B2RBD1 E1P526 Q5TD98 Q8WW21 Q9NQU2

Explore the universe of human proteins at neXtProt for FHL5: NX_Q5TD97

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q5TD97

  • FHL5 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001164278.1  NP_065228.4  

    ENSEMBL proteins: 
     ENSP00000326022   ENSP00000396390   ENSP00000442357  

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    Uscn Proteins for FHL5

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IEA--


    FHL5 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    FHL5 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR001781 Znf_LIM

    Graphical View of Domain Structure for InterPro Entry Q5TD97

    ProtoNet protein and cluster: Q5TD97

    2 Blocks protein families:
    IPB000362 Fumarate lyase
    IPB001781 Zn-binding protein


    UniProtKB/Swiss-Prot: FHL5_HUMAN, Q5TD97
    Similarity: Contains 4 LIM zinc-binding domains


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: FHL5_HUMAN, Q5TD97
    Function: May be involved in the regulation of spermatogenesis. Stimulates CREM transcriptional activity in a
    phosphorylation-independent manner

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    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003713transcription coactivator activity IEA--
    GO:0005515protein binding IPI--
    GO:0008270zinc ion binding IEA--


    FHL5 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for FHL5:
     Increased G1 DNA content 

    Animal Models:
         Mouse knock-out Fhl5tm1Saco for FHL5
         2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Fhl5):
     endocrine/exocrine gland  reproductive system 

    FHL5 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Transcription_CREM signaling in testis
    Transcription_CREM signaling in testis1.00
    Transcription CREM signaling in testis0.96

    Pathway sources
    See GeneCards unified pathways
    Show all pathways

    1 EMD Millipore Pathway for FHL5
        Transcription CREM signaling in testis


    1 GeneGo (Thomson Reuters) Pathway for FHL5
        Transcription CREM signaling in testis



    FHL5 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for FHL5

    STRING Interaction Network Preview (showing 5 interactants - click image to see 11)

    5/15 Interacting proteins for FHL5 (Q5TD971, 2, 3 ENSP000003260224) via UniProtKB, MINT, STRING, and/or I2D (see all 15)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    COILP384322, 3, ENSP000002403164MINT-2866446 I2D: score=2 STRING: ENSP00000240316
    DNAL4O960153, ENSP000002160684I2D: score=4 STRING: ENSP00000216068
    CREMQ030603, ENSP000002653724I2D: score=1 STRING: ENSP00000265372
    SRFP118313, ENSP000002653544I2D: score=1 STRING: ENSP00000265354
    ZNF446Q9NWS93, ENSP000003365654I2D: score=1 STRING: ENSP00000336565
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0045944positive regulation of transcription from RNA polymerase II promoter IEA--


    FHL5 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for FHL5
    Search CenterWatch for drugs/clinical trials and news about FHL5 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for FHL5 gene (2 alternative transcripts): 
    NM_001170807.1  NM_020482.4  

    Unigene Cluster for FHL5:

    Four and a half LIM domains 5
    Hs.632608  [show with all ESTs]
    Unigene Representative Sequence: NM_020482
    3 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000326771(uc003pos.2) ENST00000450218 ENST00000541107(uc003pot.2)


    miRNA
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    hsa-miR-3606 hsa-miR-922 hsa-miR-921 hsa-miR-143* hsa-miR-759 hsa-miR-450b-5p
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    Additional cDNA sequence: 

    AF278541.1 AK057611.1 AK058175.1 AK223539.1 AK314608.1 BC021723.2 BC029490.1 

    5 DOTS entries:

    DT.205872  DT.100747234  DT.92417613  DT.95271833  DT.92014738 

    24/51 AceView cDNA sequences (see all 51):

    BP370990 CD171872 BP369300 AI784680 NM_020482 BP370509 AK058175 AI823512 
    N92352 BI488728 BG771819 AA815365 BC029490 BI827550 BM908053 BG719109 
    BI754762 AK057611 AI424871 BP370918 BI553423 BI459075 D62998 AW771484 

    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for FHL5 (see all 6)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8 ^ 9
    SP1:                    -           -     -                                             
    SP2:                    -     -     -     -                                             
    SP3:                    -           -     -                                             
    SP4:                                -     -                                             
    SP5:                                -                                                   


    ECgene alternative splicing isoforms for FHL5

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    FHL5 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AAATAAAAGT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See FHL5 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for FHL5

    SOURCE GeneReport for Unigene cluster: Hs.632608

    UniProtKB/Swiss-Prot: FHL5_HUMAN, Q5TD97
    Tissue specificity: Testis-specific (at protein level)

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for FHL5 gene from 6/21 species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves FHL51 four and a half LIM domains 5 73.14(n)
    67.63(a)
      421803  XM_419829.2  XP_419829.1 
    lizard
    (Anolis carolinensis)
    Reptilia FHL56
    --
    66(a)
    1 ↔ 1
    1(173713959-173729810)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.136512 Transcribed sequence with weak similarity to protein more 74.94(n)    BX736019.1 
    zebrafish
    (Danio rerio)
    Actinopterygii epb496
    zgc:923756
    zgc:92375

    61(a)
    possible ortholog
    1 ↔ 1
    10(20141545-20181526)
    17(15446841-15452914)
    fruit fly
    (Drosophila melanogaster)
    Insecta CG343256
    CG319886
    (see all 4)
    --
    30(a)
    29(a)
    (see all 4)
    possible ortholog
    possible ortholog
    (see all 4)
    X(16608480-16615440)
    2L(21201535-21202742)
    worm
    (Caenorhabditis elegans)
    Secernentea lim-96
    LIM domain family member (lim-9)
    20(a)
    1 → many
    I(9182409-9210332)


    ENSEMBL Gene Tree for FHL5 (if available)
    TreeFam Gene Tree for FHL5 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for FHL5 gene
    ABLIM32  LIMS12  LIMS3L2  LDB32  LPXN2  ABLIM22  PDLIM52  ENSG000002572072  
    LIMS22  FHL22  ABLIM12  PDLIM72  EPB492  FHL32  FHL12  TGFB1I12  
    LIMS32  PXN2  
    4 SIMAP similar genes for FHL5 using alignment to 1 protein entry:     FHL5_HUMAN:
    FHL2    AAG11    FHL3    FHL1

    FHL5 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/950 NCBI SNPs in FHL5 are shown (see all 950    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 6 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1179659871,2
    F,--97008505(+) CTTCAT/CCAAAT 2 -- us2k11Minor allele frequency- C:0.03NA 120
    rs1877206371,2
    --97008604(+) ATTTGC/TATACT 2 -- us2k10--------
    rs1467559031,2
    --97008619(+) TTAATC/TTGCCT 2 -- us2k10--------
    rs1925644811,2
    --97008651(+) ATAAAC/TCTAGT 2 -- us2k10--------
    rs117563151,2
    C,F,A,H,--97008678(+) ATCTCG/ACACTT 2 -- us2k118Minor allele frequency- A:0.11NS EA NA WA 2336
    rs1821610921,2
    --97008720(+) TCTAAC/TTAAAT 2 -- us2k10--------
    rs24997761,2
    C,F,H,--97008749(+) ATGCAA/GTATAT 2 -- us2k16Minor allele frequency- G:0.04NS EA WA 540
    rs1869319731,2
    --97008790(+) ATTAAA/TATATG 2 -- us2k10--------
    rs1926824661,2
    --97008831(+) GATTTG/TGTCTG 2 -- us2k10--------
    rs1393484301,2
    --97008900(+) TTCTGC/TTCCAG 2 -- us2k10--------

    HapMap Linkage Disequilibrium report for FHL5 (97010424 - 97064512 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for FHL5: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing FHL5
    DNA2.0 Custom Variant and Variant Library Synthesis for FHL5

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    FHL5 for disorders           About GeneDecksing

    OMIM gene information: 605126    OMIM disorders: --

    9 diseases for FHL5:    About MalaCards
    hemophagocytic lymphohistiocytosis    thyroid adenoma    azoospermia    adenoma
    pharyngitis    infertility    thyroiditis    ataxia
    neuronitis

    Human Genome Epidemiology (HuGE) Navigator: FHL5 (7 documents)

    Export disorders for FHL5 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for FHL5 gene, integrated from 9 sources (see all 27):
    (articles sorted by number of sources associating them with FHL5)
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    1. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    3. The DNA sequence and analysis of human chromosome 6. (PubMed id 14574404)1, 2 Mungall A.J.... Beck S. (2003)
    4. Cloning and expression of activator of CREM in testis in human testicular tissue. (PubMed id 11327716)1, 2 Palermo I.... Travali S. (2001)
    5. Cyclic adenosine monophosphate response-element bindin g protein activation by mitogen-activated protein kinase-activated protein kinas e 3 and four-and-a-half LIM domains 5 plays a key role for vein graft intimal hy perplasia. (PubMed id 23127979)1 Nakanishi K....Sasajima T. (2013)
    6. Genome-wide association analysis identifies susceptibi lity loci for migraine without aura. (PubMed id 22683712)1 Freilinger T....van den Maagdenberg A.M. (2012)
    7. A genome-wide association study for coronary artery di sease identifies a novel susceptibility locus in the major histocompatibility co mplex. (PubMed id 22319020)1 Davies R.W....McPherson R. (2012)
    8. Evaluation of 172 candidate polymorphisms for associa tion with oligozoospermia or azoospermia in a large cohort of men of European d escent. (PubMed id 20378615)1 Aston K.I....Carrell D.T. (2010)
    9. Proteome analysis of the thalamus and cerebrospinal f luid reveals glycolysis dysfunction and potential biomarkers candidates for sch izophrenia. (PubMed id 20471030)1 Martins-de-Souza D....Turck C.W. (2010)
    10. Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis. (PubMed id 20488182)1 Wu H....Wang L. (2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 9457 HGNC: 17371 AceView: FHL5 Ensembl:ENSG00000112214 euGenes: HUgn9457
    ECgene: FHL5 H-InvDB: FHL5

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for FHL5 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for FHL5 gene:
    Search GeneIP for patents involving FHL5

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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