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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

FEN1 Gene

protein-coding   GIFtS: 72
GCID: GC11P061560

flap structure-specific endonuclease 1


(Previous symbol: RAD2)
 Explore 40 diseases affiliated with
FEN1 via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for FEN1    

Aliases
for FEN1 gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
Flap Structure-Specific Endonuclease 11 2 3     DNase IV2 3
FEN-11 2 3     Flap Endonuclease 12
MF11 2 3     Maturation Factor-12
RAD21 2 3     EC 3.1.-.-3
Maturation Factor 12 3     HFEN-11

External Ids:    HGNC: 36501   Entrez Gene: 22372   Ensembl: ENSG000001684967   OMIM: 6003935   UniProtKB: P397483   

Export aliases for FEN1 gene to outside databases

Previous GC identifers: GC11P064074 GC11P063135 GC11P061811 GC11P061335 GC11P061316 GC11P057888


Summaries
for FEN1 gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
Entrez Gene summary for FEN1:
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki
fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1
during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing
the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of
ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain
trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both
binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective
function of this protein, leading to site-specific trinucleotide expansions. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: FEN1_HUMAN, P39748
Function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA
replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by
displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap
from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch
base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as
a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and
deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H
activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA

Gene Wiki entry for FEN1 (Flap structure-specific endonuclease 1)


Genomic Views
for FEN1 gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000011.9  NC_018922.1  NT_167190.1  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the FEN1 gene promoter:
         COUP-TF1   NRSF form 1   COUP   MIF-1   HNF-4alpha2   NRSF form 2   HNF-4alpha1   COUP-TF   Meis-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: FEN1 promoter sequence
   Search SABiosciences Chromatin IP Primers for FEN1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat FEN1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11q12   Ensembl cytogenetic band:  11q12.2   HGNC cytogenetic band: 11q12

FEN1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
FEN1 gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P061560:  view genomic region     (about GC identifiers)

Start:
 61,560,109 bp from pter      End:
 61,564,716 bp from pter
Size:
 4,608 bases      Orientation:
 plus strand

Proteins
for FEN1 gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: FEN1_HUMAN, P39748 (See protein sequence)
Recommended Name: Flap endonuclease 1  
Size: 380 amino acids; 42593 Da
Cofactor: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May
bind an additional third magnesium ion after substrate binding
Subunit: Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA
monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds
EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11
Subcellular location: Nucleus, nucleolus. Nucleus, nucleoplasm. Mitochondrion (By similarity). Note=Resides mostly in
the nucleoli and relocalizes to the nucleoplasm upon DNA damage
6 PDB 3D structures from and Proteopedia for FEN1:
1U7B (3D)        1UL1 (3D)        3Q8K (3D)        3Q8L (3D)        3Q8M (3D)        3UVU (3D)    

Explore the universe of human proteins at neXtProt for FEN1: NX_P39748

Post-translational modifications:

  • Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces
    • DNA-binding activity but does not affect interaction with PCNA or EP3001
  • Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs
    • during late S-phase and results in dissociation from PCNA1
  • Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_P39748

    • FEN1 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_004102.1  
    ENSEMBL proteins: 
     ENSP00000305480   ENSP00000445692   ENSP00000460402  
    Reactome Protein details: P39748
    Human Recombinant Protein Products: 
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    Enzo Life Sciences proteins for FEN1
    OriGene Purified Protein: FEN1
    OriGene Protein Over-expression Lysate: FEN1
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    Novus Biologicals FEN1 Proteins
    Novus Biologicals FEN1 Lysates
    Browse Sino Biological Recombinant Proteins
    ProSpec Recombinant Protein for FEN1
    Uscn Proteins for FEN1

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005730nucleolus IEA--
    GO:0005739mitochondrion IDA18995831


    FEN1 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for FEN1

    Protein Domains /
    Families
    for FEN1 gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    FEN1 for domains           About GeneDecksing

    5/7 InterPro domains/families (see all 7):
     IPR006084 XPG/Rad2
     IPR023426 Flap_endonuc
     IPR006086 XPG-I_dom
     IPR006085 XPG_DNA_repair_N
     IPR019974 XPG_CS

    Graphical View of Domain Structure for InterPro Entry P39748

    ProtoNet protein and cluster: P39748

    2 Blocks protein families:
    IPB006084 Xeroderma pigmentosum group G/yeast RAD superfamily signature
    IPB008918 Helix-hairpin-helix motif


    UniProtKB/Swiss-Prot: FEN1_HUMAN, P39748
    Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily


    Function
    for FEN1 gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: FEN1_HUMAN, P39748
    Function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA
    replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by
    displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap
    from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch
    base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as
    a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and
    deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H
    activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA

         Genatlas biochemistry entry for FEN1:
    flap structure specific endonuclease 1,with an 5'- 3' exonuclease activity,involved in long patch base excision
    repair,DNA replication,where it processes Okazaki fragments,recombination and repair where it interacts with PCNA
    complex and DNA and acts as an exonuclease

    Enzyme Number (IUBMB): EC 3.1.-.-1

    miRNA
    Products:            		miRTarBase miRNAs that target FEN1:
    hsa-mir-24 (MIRT000116)

    OriGene 3'-UTR Clone: FEN1
    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat FEN1
    7 QIAGEN miScript miRNA Assays for microRNAs that regulate FEN1:
    hsa-miR-140-5p hsa-miR-4324 hsa-miR-544b hsa-miR-610 hsa-miR-4251 hsa-miR-378* hsa-miR-4293
    SwitchGear 3'UTR luciferase reporter plasmid: FEN1 3' UTR sequence
    Inhib. RNA
    Products:            		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for FEN1 (see all 7)
    OriGene shRNA RFP: FEN1
    OriGene siRNA: FEN1
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat FEN1

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for FEN1

    Clone
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    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for FEN1 (see all 3)
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    GenScript: all cDNA clones in your preferred vector: FEN1 (NM_004111)
    Browse Sino Biological Human cDNA Clones
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat FEN1 

    Cell Line
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    Search LifeMap BioReagents cell lines for FEN1

    In Situ Assay
    Products:       
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for FEN1

    Gene Ontology (GO): 5/13 molecular function terms (GO ID links to tree view) (see all 13):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000287magnesium ion binding IEA--
    GO:0003677DNA binding IMP11986308
    GO:0003684damaged DNA binding TAS8007985
    GO:0003690double-stranded DNA binding TAS8131753
    GO:0004519endonuclease activity TAS8131753


    FEN1 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for FEN1:
     Decreased DCP1a protein expres 

    Animal Models:
         Mouse knock-outs for FEN1: Fen1tm1Rak Fen1tm1Klng Fen1tm1Bew
         15 MGI mutant phenotypes (inferred from 6 alleles) (MGI details for Fen1):
     adipose tissue  cellular  digestive/alimentary  embryogenesis  endocrine/exocrine gland 
     growth/size  hematopoietic system  homeostasis/metabolism  immune system  liver/biliary system 
     mortality/aging  nervous system  normal  respiratory system  tumorigenesis 

    FEN1 for phenotypes           About GeneDecksing


    Pathways & Interactions
    for FEN1 gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/11 super-pathways (see all 11) About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Lagging Strand Synthesis
    8/10 pathways (see all 10)
    		Lagging Strand Synthesis1.00
    		DNA strand elongation0.65
    		Telomere C-strand (Lagging Strand) Synthesis0.91
    		DNA replication0.56
    		Extension of Telomeres0.83
    		Processive synthesis on the C-strand of the telomere0.55
    		Processive synthesis on the lagging strand0.75
    		Removal of the Flap Intermediate from the C-strand0.50
    2Resolution of Abasic Sites (AP sites)
    		Resolution of Abasic Sites (AP sites)1.00
    		Resolution of AP sites via the multiple-nucleotide patch replacement pathway0.89
    		Base Excision Repair1.00
    		Base excision repair0.51
    		Removal of DNA patch containing abasic residue0.89
    3Non-homologous end-joining
    		Non-homologous end-joining1.00
    		DNA damage NHEJ mechanisms of DSBs repair0.36
    		DNA damage_NHEJ mechanisms of DSBs repair0.36
    4Ubiquitinated Orc1 is degraded by the proteasome
    		Synthesis of DNA0.51
    		S Phase0.40
    		DNA Replication0.49
    5Cell Cycle
    		Cell Cycle1.00
    		Cell Cycle, Mitotic0.84

    Pathway sources
    See GeneCards unified pathways
    Show all pathways

    2 EMD Millipore Pathways for FEN1
        Cell cycle Transition and termination of DNA replacation
    DNA damage NHEJ mechanisms of DSBs repair

    2 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for FEN1
        Mismatch Repair in Eukaryotes
    DNA Repair Mechanisms

    2 Cell Signaling Technology (CST) Pathways for FEN1
        Cell Cycle / Checkpoint Control
    DNA Damage

    2 GeneGo (Thomson Reuters) Pathways for FEN1
        DNA damage NHEJ mechanisms of DSBs repair
    Cell cycle Transition and termination of DNA replication

    5/20        Reactome Pathways for FEN1 (see all 20)
        Extension of Telomeres
    Processive synthesis on the lagging strand
    Telomere Maintenance
    DNA Repair
    DNA Replication


    3         Kegg Pathways  (Kegg details for FEN1):
        DNA replication
    Base excision repair
    Non-homologous end-joining


    FEN1 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for FEN1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/913 Interacting proteins for FEN1 (P397481, 2, 3 ENSP000003054804) via UniProtKB, MINT, STRING, and/or I2D (see all 913)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=1 
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    HIST1H4DP628053I2D: score=1 
    HIST1H4EP628053I2D: score=1 
    About this table

    Gene Ontology (GO): 5/14 biological process terms (GO ID links to tree view) (see all 14):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000084S phase of mitotic cell cycle TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0000722telomere maintenance via recombination TAS--
    GO:0000723telomere maintenance TAS--
    GO:0006260DNA replication NAS15504738


    FEN1 for ontologies           About GeneDecksing



    Drugs & Compounds
    for FEN1 gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    FEN1 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for FEN1
    10/13 Novoseek chemical compound relationships for FEN1 gene (see all 13)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    methylmethanesulfonate 60.5 7 11687589 (2), 19596913 (1)
    oligonucleotide 28 1 11601988 (1)
    alanine 4.38 5 15131255 (1), 8621570 (1), 11053418 (1)
    calcium 3.01 2 11078214 (1), 17656006 (1)
    lipid 2.42 1 11090277 (1)
    magnesium 0 7 11258937 (4), 9880491 (2), 8247134 (1)
    atp 0 1 15579905 (1)
    phospholipid 0 1 12225777 (1)
    adp 0 1 9679139 (1)
    arginine 0 2 9305916 (1), 16912194 (1)

    Search CenterWatch for drugs/clinical trials and news about FEN1 

    Transcripts
    for FEN1 gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for FEN1 gene: 
    NM_004111.5  

    Unigene Cluster for FEN1:

    Flap structure-specific endonuclease 1
    Hs.409065  [show with all ESTs]
    Unigene Representative Sequence: NM_004111
    3 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000305885(uc001nsg.3) ENST00000535723 ENST00000535307

    miRNA
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    hsa-miR-140-5p hsa-miR-4324 hsa-miR-544b hsa-miR-610 hsa-miR-4251 hsa-miR-378* hsa-miR-4293
    SwitchGear 3'UTR luciferase reporter plasmid: FEN1 3' UTR sequence
    Inhib. RNA
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    OriGene siRNA: FEN1
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    Clone
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    Primer
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      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat FEN1

    Additional cDNA sequence: 

    AK301743.1 AK312761.1 BC000323.2 BT019524.1 CR536562.1 L37374.1 X76771.1 

    23 DOTS entries:

    DT.100815303  DT.95364169  DT.100815307  DT.455329  DT.100815314  DT.120739964  DT.86858976  DT.100771302 
    DT.97853756  DT.120740130  DT.120740129  DT.100815305  DT.100815306  DT.121390749  DT.100815302  DT.100815311 
    DT.100815313  DT.120740117  DT.91777025  DT.95364166  DT.100815301  DT.70104817  DT.95364167 

    24/427 AceView cDNA sequences (see all 427):

    BX443166 AI832123 BU538692 BE312466 BQ061097 AI824227 BU860300 AU131462 
    BU553925 BU165752 AW192592 BM561765 BQ070617 BU535646 NM_004111 BQ069168 
    CF131987 CB151489 AA306861 BQ278519 BE794549 BG825257 AA337995 BF794546 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for FEN1    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b · 3c
    SP1:                    -                           
    SP2:                    -                           
    SP3:                                                


    ECgene alternative splicing isoforms for FEN1

    Expression
    for FEN1 gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    FEN1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CGCTGTTTTT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    FEN1 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    PureStem™ progenitor E44 (Embryonic Progenitor Cell)
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See FEN1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for FEN1

    SOURCE GeneReport for Unigene cluster: Hs.409065
        SABiosciences Expression via Pathway-Focused PCR Arrays including FEN1: 
              DNA Damage Signaling Pathway in human mouse rat
              DNA Repair in human mouse rat

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    Orthologs
    for FEN1 gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of eukaryotes.

    Orthologs for FEN1 gene from 9/39 species (see all 39)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    chicken
    (Gallus gallus)    		 Aves FEN11 flap structure-specific endonuclease 1 79.91(n)
    84.47(a)    		   769677  NM_001079491.1  NP_001072959.1 
    lizard
    (Anolis carolinensis)    		 Reptilia FEN16
    		 --
    		 78(a)
    		 1 ↔ 1
    		 GL343235.1(1794007-1795155)
    African clawed frog
    (Xenopus laevis)    		 Amphibia fen1-B2 flap endonuclease 1 76.84(n)    U68141.1 
    zebrafish
    (Danio rerio)    		 Actinopterygii fen12 flap structure-specific endonuclease 1 75.87(n)   386707  AY391423.1 
    fruit fly
    (Drosophila melanogaster)    		 Insecta Fen11 , 3 DNA repair endonuclease3
    Flap endonuclease 11    		 65(a)3
    62.89(n)1
    63.68(a)1    		   53D143
    368871  NM_079041.11  NP_523765.11 
    worm
    (Caenorhabditis elegans)    		 Secernentea Y47G6A.83
    crn-11    		 endonuclease3
    Protein CRN-11    		 64(a)3
    61.9(n)1
    62.96(a)1    		   I(3482031-3484366)3
    1719171  NM_058767.51  NP_491168.11 
    baker's yeast
    (Saccharomyces cerevisiae)    		 Saccharomycetes RAD27(YKL113C)4
    RAD271    		 5' to 3' exonuclease, 5' flap endonuclease, required more4
    Rad27p1    		 57.65(n)1
    60.38(a)1    		   11(225875-224727)4
    8537471, 4  NP_012809.11, 4 
    thale cress
    (Arabidopsis thaliana)    		 eudicotyledons AT5G266801 flap endonuclease-1 57.47(n)
    55.7(a)    		   832721  NM_001161268.1  NP_001154740.1 
    rice
    (Oryza sativa)    		 Liliopsida Os.44692 Oryza sativa (japonica cultivar-group) OsFEN-1a mRNA more 72.3(n)    AB021666.2 


    ENSEMBL Gene Tree for FEN1 (if available)
    TreeFam Gene Tree for FEN1 (if available) 

    Paralogs
    for FEN1 gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for FEN1 gene
    GEN12  
    1 SIMAP similar gene for FEN1 using alignment to 5 protein entries:     FEN1_HUMAN (see all proteins):
    GEN1

    FEN1 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for FEN1
    PGOHUM00000244791


    Genomic Variants
    for FEN1 gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/73 NCBI SNPs in FEN1 are shown (see all 73)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 11 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs1845696851,2
    		--61562197(+) GAGTGA/GTGGCG 1 -- int10--------
    rs1902888361,2
    		--61562201(+) GGTGGC/TGAGAA 1 -- int10--------
    rs32188371,2
    		C,F,H,--61562224(+) AAGACT/GTGAAA 1 -- int15Minor allele frequency- G:0.01NS EA 594
    rs1923249261,2
    		--61562589(+) AGCACA/GTGGTT 1 -- int10--------
    rs1844395851,2
    		--61562622(+) CTTACG/TATTGA 1 -- int10--------
    rs32188381,2
    		H--61562776(+) CTTTCT/GCCTTT 1 -- int1 trp35Minor allele frequency- G:0.00NS EA 580
    rs3934871,2
    		C,H--61562805(+) CTTTCT/CTTTTT 1 -- int1 trp36Minor allele frequency- C:0.00MN NS EA NA 655
    rs1478818141,2
    		C,--61562917(+) TTTGGC/TCGTAA 2 G syn11Minor allele frequency- T:0.00NA 4548
    rs1404588641,2
    		C,F--61562973(+) TGTTCA/G/TCCAGG 3 H R L mis12NA EU 5875
    rs1895888181,2
    		C,--61562980(+) CAGGGG/TGGGGA 2 G syn10--------

    HapMap Linkage Disequilibrium report for FEN1 (61560109 - 61564716 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for FEN1: --
    Human Gene Mutation Database (HGMD): FEN1

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    Disorders / Diseases
    for FEN1 gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    FEN1 for disorders           About GeneDecksing

    OMIM gene information: 600393    OMIM disorders: --

    20/40 diseases for FEN1 (see all 40):    About MalaCards
    xeroderma pigmentosum, group g    xeroderma pigmentosum    vitelliform macular dystrophy    graft versus host disease
    adenomatous polyposis coli    traumatic brain injury    macular dystrophy    brain injury
    bloom syndrome    werner syndrome    systemic lupus erythematosus    lupus erythematosus
    herpes simplex    huntington's disease    cockayne syndrome    polyposis
    myelofibrosis    multiple sclerosis    genetic disease    colorectal cancer

    2 diseases from the University of Copenhagen DISEASES database for FEN1:
    Autosomal recessive disease     Vitelliform macular dystrophy

    10/15 Novoseek disease relationships for FEN1 gene (see all 15)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    werner syndrome 68.2 3 15592449 (1), 11598021 (1), 14688284 (1)
    bloom syndrome 59.5 2 16326861 (1), 14688284 (1)
    macular dystrophy, vitelliform 52.3 4 10769175 (1)
    sezary syndrome 33.3 2 9613981 (1), 8695857 (1)
    xeroderma pigmentosum 29.2 2 7989298 (1), 9305916 (1)
    huntington disease 19.6 1 12917330 (1)
    promyelocytic leukemia 15.6 2 9894157 (1), 10771101 (1)
    microsatellite instability 5.17 3 8782829 (1), 14562054 (1)
    cancer 5.04 26 19010819 (4), 17589521 (3), 19068479 (1), 16879693 (1) (see all 9)
    shock 0 5 15942332 (2), 14673135 (1), 11275689 (1), 15152009 (1)

    Genetic Association Database (GAD): FEN1
    Human Genome Epidemiology (HuGE) Navigator: FEN1 (12 documents)

    Export disorders for FEN1 gene to outside databases

    Publications
    for FEN1 gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for FEN1 gene, integrated from 9 sources (see all 260):
    (articles sorted by number of sources associating them with FEN1)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human. (PubMed id 7774922)1, 2, 3, 9 Hiraoka L.R.... Hsieh C.-L. (1995)
    2. Structural basis for recruitment of human flap endonuclease 1 to PCNA. (PubMed id 15616578)1, 2, 9 Sakurai S.... Hakoshima T. (2005)
    3. Regulation of human flap endonuclease-1 activity by acetylation through the transcriptional coactivator p300. (PubMed id 11430825)1, 2, 9 Hasan S.... Hottiger M.O. (2001)
    4. The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21. (PubMed id 9305916)1, 2, 9 Gary R.... Park M.S. (1997)
    5. Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damage. (PubMed id 8007985)1, 2, 9 Murray J.M.... Watts F.Z. (1994)
    6. The 'flap' endonuclease gene FEN1 is excluded as a candidate gene implicated in the CAG repeat expansion underlying Huntington disease. (PubMed id 11260214)1, 4, 9 Otto C.J....Andrew S.E. (2001)
    7. Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1. (PubMed id 18499658)1, 2, 9 Farina A....Hurwitz J. (2008)
    8. Essential amino acids for substrate binding and catalysis of human flap endonuclease 1. (PubMed id 8621570)1, 2, 9 Shen B.... Park M.S. (1996)
    9. Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-delta p66 subunit and flap endonuclease-1. (PubMed id 15576034)1, 2, 9 Bruning J.B. and Shamoo Y. (2004)
    10. Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding. (PubMed id 20729856)1, 2 Guo Z....Shen B. (2010)

    External Searches for FEN1 gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing FEN1 gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 2237 HGNC: 3650 AceView: FEN1 Ensembl:ENSG00000168496 euGenes: HUgn2237
    ECgene: FEN1 Kegg: 2237 H-InvDB: FEN1

    Other Databases showing FEN1 gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing FEN1 gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for FEN1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for FEN1 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/fen1/

    Intellectual Property
    for FEN1 gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for FEN1 gene:
    Search GeneIP for patents involving FEN1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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