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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

FBXO21 Gene

protein-coding   GIFtS: 50
GCID: GC12M117581

F-box protein 21

(Previous name: F-box only protein 21 )
 Explore 1 disease affiliated with
FBXO21 via our new
 Human Malady Compendium 
Biological research products
for FBXO21
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
F-Box Protein 211 2
FBX211 2 3 5
KIAA08751 3 5
F-Box Only Protein 211 2

External Ids:    HGNC: 135921   Entrez Gene: 230142   Ensembl: ENSG000001351087   OMIM: 6090955   UniProtKB: O949523   

Export aliases for FBXO21 gene to outside databases

Previous GC identifers: GC12P116427 GC12M117102 GC12M117364 GC12M115994 GC12M115995 GC12M116044 GC12M114590


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for FBXO21:
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif,
the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs
(SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3
classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different
protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs
class. Alternative splicing of this gene generates 2 transcript variants. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: FBX21_HUMAN, O94952
Function: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex (By
similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_009775.17  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the FBXO21 gene promoter:
         GR   Elk-1   Sox5   STAT1   STAT1beta   LUN-1   STAT1alpha   AREB6   ZID   GR-alpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidFBXO21 promoter sequence
   Search SABiosciences Chromatin IP Primers for FBXO21

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat FBXO21


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q24.22   Ensembl cytogenetic band:  12q24.22   HGNC cytogenetic band: 12q24.23

FBXO21 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
FBXO21 gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M117581:  view genomic region     (about GC identifiers)

Start:
117,581,146 bp from pter      End:
117,628,336 bp from pter
Size:
47,191 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: FBX21_HUMAN, O94952 (See protein sequence)
Recommended Name: F-box only protein 21  
Size: 628 amino acids; 72270 Da
Subunit: Directly interacts with SKP1 and CUL1 (By similarity)
Sequence caution: Sequence=CAB66833.2; Type=Erroneous termination; Positions=81; Note=Translated as Trp;
Secondary accessions: B3KMF0 Q5BJG0 Q9H087
Alternative splicing: 2 isoforms:  O94952-2   O94952-1   (May be due to a competing acceptor splice site. No experimental confirmation available)

Explore the universe of human proteins at neXtProt for FBXO21: NX_O94952

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_O94952

  • FBXO21 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_055817.1  NP_296373.1  

    ENSEMBL proteins: 
     ENSP00000414468   ENSP00000449264   ENSP00000328187   ENSP00000446973  

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    Uscn Proteins for FBXO21

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000151ubiquitin ligase complex NAS10531035


    FBXO21 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    FBXO21 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR001810 F-box_dom_cyclin-like
     IPR011722 Hemimethylated_DNA-bd_dom

    Graphical View of Domain Structure for InterPro Entry O94952

    ProtoNet protein and cluster: O94952

    UniProtKB/Swiss-Prot: FBX21_HUMAN, O94952
    Similarity: Contains 1 F-box domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: FBX21_HUMAN, O94952
    Function: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex (By
    similarity)

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    hsa-miR-579 hsa-miR-4254 hsa-miR-106a hsa-miR-15a hsa-miR-128 hsa-miR-3916 hsa-miR-93 hsa-miR-3613-3p
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding IEA--
    GO:0004842ubiquitin-protein ligase activity NAS10531035


    FBXO21 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for FBXO21:
     Decreased p24 protein expressi 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for FBXO21

    STRING Interaction Network Preview (showing 5 interactants - click image to see 7)

    5/8 Interacting proteins for FBXO21 (O949523 ENSP000003281874) via UniProtKB, MINT, STRING, and/or I2D (see all 8)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TERF1P542743, ENSP000002766034I2D: score=1 STRING: ENSP00000276603
    CNTNAP4Q9C0A03, ENSP000004176284I2D: score=2 STRING: ENSP00000417628
    TERF2Q155543, ENSP000002549424I2D: score=1 STRING: ENSP00000254942
    UBCENSP000003448184STRING: ENSP00000344818
    CNTNAP2ENSP000003547784STRING: ENSP00000354778
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006511ubiquitin-dependent protein catabolic process NAS10531035
    GO:0016567protein ubiquitination NAS10531035


    FBXO21 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for FBXO21
    Search CenterWatch for drugs/clinical trials and news about FBXO21 / FBX21 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for FBXO21 gene (2 alternative transcripts): 
    NM_015002.2  NM_033624.2  

    Unigene Cluster for FBXO21:

    F-box protein 21
    Hs.740376  [show with all ESTs]
    Unigene Representative Sequence: NM_033624
    7 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000427718(uc001twj.3 uc001twk.3 uc009zwq.3) ENST00000550180
    ENST00000330622 ENST00000547806 ENST00000548840 ENST00000549689 ENST00000551458


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    8/74 QIAGEN miScript miRNA Assays for microRNAs that regulate FBXO21 (see all 74):
    hsa-miR-579 hsa-miR-4254 hsa-miR-106a hsa-miR-15a hsa-miR-128 hsa-miR-3916 hsa-miR-93 hsa-miR-3613-3p
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    Inhib. RNA
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    Additional cDNA sequence: 

    AB020682.1 AF174601.1 AK001699.1 AK074714.1 AK308141.1 AK314573.1 AL136899.1 BC034045.1 
    BC042333.1 BC091496.1 

    15 DOTS entries:

    DT.448486  DT.91753090  DT.95355341  DT.95145079  DT.100822829  DT.101967466  DT.121151082  DT.97859016 
    DT.95073284  DT.100665658  DT.121151006  DT.121151089  DT.100702617  DT.101966680  DT.92032018 

    24/349 AceView cDNA sequences (see all 349):

    AI867471 N52366 CD678471 BI792373 BU688948 BF211311 AI560535 T10762 
    BU681154 CD518201 AA317076 CD364571 AA444045 AI376852 W72224 CB132899 
    CA866157 BX112550 AI566208 AI223861 CB119361 AW663975 BG743296 AI209113 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for FBXO21    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11a · 11b · 11c ^ 12 ^ 13
    SP1:                    -                                                     -                           
    SP2:                    -                                         -           -                           
    SP3:                                                                                                      
    SP4:                                                                                                      
    SP5:                                                                                                      


    ECgene alternative splicing isoforms for FBXO21

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    FBXO21 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GGGCTCCAGG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    FBXO21 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    EyeRetinaEye
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See FBXO21 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for FBXO21

    SOURCE GeneReport for Unigene cluster: Hs.740376
        SABiosciences Expression via Pathway-Focused PCR Array including FBXO21: 
              Ubiquitin Ligases in human mouse rat

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for FBXO21

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for FBXO21 gene from 5/14 species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Fbxo211 , 5 F-box protein 211, 5 87.67(n)1
    95.69(a)1
      5 (57.73 cM)5
    2316701  NM_145564.31  NP_663539.11 
     1179767705 
    chicken
    (Gallus gallus)
    Aves FBXO211 F-box protein 21 75.88(n)
    79.56(a)
      427722  XM_425297.2  XP_425297.2 
    lizard
    (Anolis carolinensis)
    Reptilia FBXO216
    --
    73(a)
    1 ↔ 1
    LGb(2396371-2410832)
    zebrafish
    (Danio rerio)
    Actinopterygii fbxo211 F-box protein 21 63.32(n)
    61.16(a)
      567738  XM_691038.3  XP_696130.3 
    honey bee
    (Apis mellifera)
    Insecta --
    --
    30(a)
    1 ↔ 1
    GroupUn.89(14598-17737)


    ENSEMBL Gene Tree for FBXO21 (if available)
    TreeFam Gene Tree for FBXO21 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/986 NCBI SNPs in FBXO21 are shown (see all 986    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1835999931,2
    --117581140(+) CATAGA/TGTCAA 2 -- ds50010--------
    rs1866893251,2
    --117581144(+) GAGTCA/CATTGA 2 -- ds50010--------
    rs1169604291,2
    F,--117581146(+) GTCAAT/CTGACT 2 -- ds50011Minor allele frequency- C:0.02EA 120
    rs37414701,2
    C,F,A,H,--117581158(-) TAGGTT/CAAACA 2 -- ds500120Minor allele frequency- C:0.28EA NS NA CSA 3724
    rs1497252841,2
    --117581265(+) ATCAAC/TGGCAA 2 -- ds50010--------
    rs1930083681,2
    --117581725(+) TTTTTA/GAAATG 2 -- ut310--------
    rs1484909421,2
    --117582213(+) TTAAAC/TAGCTC 2 -- ut310--------
    rs37414681,2
    C,F,H,--117582242(-) ATTTGG/TCCCCT 2 -- ut31 ese312Minor allele frequency- T:0.13EA NS WA NA 2438
    rs1857788891,2
    --117582259(+) CTCTTC/TCTAGT 2 -- ut310--------
    rs1898580621,2
    --117582263(+) TTCTAA/GTAACT 2 -- ut310--------

    HapMap Linkage Disequilibrium report for FBXO21 (117581146 - 117628336 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for FBXO21
         1 CNV: 4789

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing FBXO21
    DNA2.0 Custom Variant and Variant Library Synthesis for FBXO21

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    FBXO21 for disorders           About GeneDecksing

    OMIM gene information: 609095    OMIM disorders: --

    1 disease for FBXO21:    About MalaCards
    mast cell neoplasm

    1 disease from the University of Copenhagen DISEASES database for FBXO21:
    Mast cell neoplasm
    Human Genome Epidemiology (HuGE) Navigator: FBXO21 (1 document)

    Export disorders for FBXO21 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for FBXO21 gene, integrated from 9 sources (see all 18):
    (articles sorted by number of sources associating them with FBXO21)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification of a family of human F-box proteins. (PubMed id 10531035)1, 2, 3 Cenciarelli C....Pagano M. (1999)
    2. Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10048485)1, 2, 3 Nagase T....Ohara O. (1998)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PubMed id 11230166)1, 2 Wiemann S.... Poustka A. (2001)
    6. Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
    7. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
    8. Proliferating cell nuclear antigen (PCNA)-associated KIAA0101/PAF15 protein is a cell cycle-regulated anaphase-promoting complex/cyc losome substrate. (PubMed id 21628590)1 Emanuele M.J....Elledge S.J. (2011)
    9. Ubiquitin ligase substrate identification through qua ntitative proteomics at both the protein and peptide levels. (PubMed id 21987572)1 Lee K.A....Doedens J.R. (2011)
    10. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 23014 HGNC: 13592 AceView: FBXO21 Ensembl:ENSG00000135108 euGenes: HUgn23014
    ECgene: FBXO21 H-InvDB: FBXO21

    (According to HUGE)
    About This Section
    HUGE: KIAA0875

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for FBXO21 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for FBXO21 gene:
    Search GeneIP for patents involving FBXO21

    GeneCards and IP:
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