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FBLN2 Gene

protein-coding   GIFtS: 62

GC03P013565
fibulin 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FIBL-2 3
Descriptions
fibulin 2 2
External Ids
HGNC: 36011
Entrez Gene: 21992
UniProtKB: P980953
Ensembl: ENSG000001635207
Search outside databases for aliases for FBLN2 gene

Previous GC identifer: GC03P013541

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for FBLN2:
This gene encodes an extracellular matrix protein, which belongs to the fibulin family. This
protein binds various extracellular ligands and calcium. It may play a role during organ
development, in particular, during the differentiation of heart, skeletal and neuronal structures.
Alternatively spliced transcript variants encoding different isoforms have been identified.
[provided by RefSeq]

UniProtKB/Swiss-Prot: FBLN2_HUMAN, P98095
Function: Its binding to fibronectin and some other ligands is calcium dependent

Gene Wiki entry for FBLN2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the FBLN2 gene  

Entrez Gene cytogenetic band: 3p25.1   Ensembl cytogenetic band:  3p25.1   HGNC cytogenetic band: 3p25-p24

FBLN2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P013565:     (about GC identifiers)

Start:
13,565,625 bp from pter
End:
13,654,923 bp from pter
Size:
89,299 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000003.10  NT_022517.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: FBLN2_HUMAN, P98095 (See protein sequence)
Recommended Name: Fibulin-2 precursor  
Size: 1184 amino acids; 126573 Da
Subunit: Homotrimer; disulfide-linked. Interacts with LAMA2 (By similarity)
Subcellular location: Secreted, extracellular space, extracellular matrix
Developmental stage: Widely expressed during embryonic development. Primarily detected within the
neuropithelium, spinal ganglia and peripheral nerves

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001004019.1  NP_001989.2  


    ENSEMBL proteins: 
    ENSP00000384169 ENSP00000295760 ENSP00000295761 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Fibulin 2)
    Human Recombinant Proteins from Abnova (FBLN2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region IEA--
    GO:0005578 proteinaceous extracellular matrix TAS7806230
    About this table

    Antibodies for FBLN2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for FBLN2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Fibulin 2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (FBLN2)
    Novus Biologicals Antibodies for FBLN2

    Assays for FBLN2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/8 InterPro domains/families (see all 8 ):
     IPR001881 EGF_Ca_bd
     IPR000742 EGF_3
     IPR000020 Anaphylatoxin/fibulin
     IPR013032 EGF-like_reg_CS
     IPR000152 EGF-type_Asp/Asn_hydroxyl_CS


       GeneDecks  FBLN2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P98095

    ProtoNet protein and cluster: P98095

    2 Blocks protein families:
    IPB000152 Aspartic acid and asparagine hydroxylation site
    IPB001881 EGF-like calcium-binding


    UniProtKB/Swiss-Prot: FBLN2_HUMAN, P98095
    Similarity: Belongs to the fibulin family
    Similarity: Contains 3 anaphylatoxin-like domains
    Similarity: Contains 11 EGF-like domains

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (FBLN2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (FBLN2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001004019

                  Applied Biosystems Silencer® siRNAs for FBLN2

                  Sigma-Aldrich siRNA for FBLN2  
                         Sigma-Aldrich shRNA Panels and shRNA for FBLN2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001004019
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001004019
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001004019 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001004019

    UniProtKB/Swiss-Prot: FBLN2_HUMAN, P98095
    Function: Its binding to fibronectin and some other ligands is calcium dependent

    Genatlas biochemistry entry for FBLN2:
    fibulin 2,calcium binding,extracellular matrix protein,expressed in heart tissue,located in the
    embryonic endocardial cushion tissue and predominantly in the valves,also expressed in
    skin,perichondrium,elastic intima of blood vessels and kidney glomerulus and colocalizing with
    FBN1 in these tissues with different variants

    1 MGI mutant phenotype (inferred from 1 allele(MGI details for Fbln2):


    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005201 extracellular matrix structural constituent TAS7806230
    GO:0005509 calcium ion binding TAS7806230
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    1 Invitrogen iPath™ Online BioAtlas - Pathway for FBLN2 (Maps provided by GeneGo):
     Cell-matrix glycoconjugates

       GeneDecks  FBLN2 for the pathways selected above  
    About GeneDecksing

    1 Millipore Pathway for FBLN2
     Cell adhesion Cell-matrix glycoconjugates

       GeneDecks  FBLN2 for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  FBLN2 


    5/18 Interacting proteins for FBLN2 (P980952 ENSP000002957613) via UniProtKB, MINT, and/or STRING (see all 18 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ATN1P542592MINT-2858818
    HSPG2ENSP000003638273STRING (score=.991)
    FN1ENSP000003235343STRING (score=.972)
    ELNENSP000003518073STRING (score=.939)
    NID1ENSP000002641873STRING (score=.929)
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for FBLN2
    3 Novoseek chemical compound relationships for FBLN2 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    neurocan 70.31 1 11038354 (1)
    calcium 8.65 3 8737292 (1), 9299350 (1), 8245130 (1)
    fibrinogen 5.76 1 7543056 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (FBLN2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (FBLN2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001004019

                  Sigma-Aldrich siRNA for FBLN2  
                         Sigma-Aldrich shRNA Panels and shRNA for FBLN2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001004019  NM_001998  

    REFSEQ mRNAs for FBLN2 gene (2 alternative transcripts): 

    NM_001004019.1   NM_001998.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001004019  NM_001998  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001004019
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001004019
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001004019 

    Additional cDNA sequence: 

    AK129883.1 AK300913.1 AK304777.1 AK304827.1 AL050095.1 AL080171.1 AY130459.1 BC051690.1 
    CR624008.1 X82494.1 

    6 DOTS entries:

    DT.91905714  DT.454137  DT.100654133  DT.100792594  DT.120883977  DT.100703619 

    24/208 AceView cDNA sequences (see all 208 ):

    BU859521 BU855789 AI193253 BM784974 BM673717 BU553532 AI379322 BU846307 
    BU556734 CR624008 BM746486 NM_001004019 NM_001998 AI246349 BU556575 BG012914 
    BU855098 AI354814 BP344809 CD515030 AA451985 BU553983 BU931668 BU844464 

    highest scoring ESTs for FBLN2:

    X82494 AA039682 AA369679 AA441817 AA441879 AA451985 AA452840 AA452981 AA479274 AA593838 

    Unigene Cluster for FBLN2:

    Fibulin 2
    Hs.198862  [show with all ESTs]
    Unigene Representative Sequence: NM_001004019


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for FBLN2

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19
    SP1:                                -                                                                                             
    SP2:                                -                                   -                                                         
    SP3:                                                                                                                              
    SP4:                                                                                                                              

    About this scheme

    ECgene alternative splicing isoforms for FBLN2

    3 Ensembl transcripts including schematic representations:
    ENST00000404922  ENST00000295760  ENST00000295761  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    FBLN2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for FBLN2

    1 / 2 / 3

    3 probe-sets matching FBLN2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    32783_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 X82494 1.00 1.00 1.00 1

    203886_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_001998 0.60 1.00 0.82 1

    203886_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  FBLN2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TACATTATAA

    SOURCE GeneReport for Unigene cluster: Hs.198862

    Expression variation in blood from EXPOLDB for FBLN2

    UniProtKB/Swiss-Prot: FBLN2_HUMAN, P98095
    Tissue specificity: Component of both basement membranes and other connective tissues. Expressed in
    heart, placenta and ovary

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for FBLN2 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    FBLN21   -- fibulin 2 85.73(n)
    86.28(a)
    484634  XM_541748.2  XP_541748.2 
    cow
    (Bos taurus)
    FBLN21   -- fibulin 2 83.97(n)
    84.11(a)
    511854  XM_589271.3  XP_589271.2 
    rat
    (Rattus norvegicus)
    Fbln21   -- fibulin 2 82.24(n)
    82.65(a)
    282583  XM_232197.4  XP_232197.4 
    mouse
    (Mus musculus)
    Fbln21, 5 6 (37.20 cM)5
    fibulin 21, 5 82.15(n)1
    83.14(a)1
    141151  NM_007992.21  NP_032018.21 
     AA1076635  AF1352385  (see all 30)
    chicken
    (Gallus gallus)
    FBLN21   -- fibulin 2 60.98(n)
    57.68(a)
    427583  XM_425156.2  XP_425156.2 
    About this table        Species with no ortholog for FBLN2

    ENSEMBL Gene Tree for FBLN2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for FBLN2 gene
    FBLN12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/648 NCBI SNPs in FBLN2 are shown (see all 648 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 258)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 3 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs37963181,2
    A,C,F,H13588016(-) GGATGT/GTGTGG 2 T/N mis1 ese38Minor allele frequency- G:0.31EU EA WA 838
    rs37326661,2
    A,C,F,H13587937(-) CAGGCC/TCGGTG 2 S/G mis112Minor allele frequency- T:0.47EA EU WA NA 2458
    rs98433441,2
    A,C,F,H13645537(+) ATGGGG/ACCAAG 2 A/T mis14Minor allele frequency- A:0.16EU EA WA 420
    rs129681,2
    A,C,F,H13654709(+) CGCGAC/ACATGT 2 -- ut31 ese39Minor allele frequency- A:0.18MN EU EA WA NA 928
    rs75961,2
    A,C,F13654856(-) TTTTTA/TTATAA 2 -- ut31 ese31Minor allele frequency- T:0.43MN 184
    --
    rs78201,2
    A,C,F13654830(+) TTTAAT/CTATAA 2 -- ut314Minor allele frequency- C:0.20MN NA 304
    rs10613981,2
    A,F,H13654696(+) AGGACC/AAGAGA 2 -- ut31 ese35Minor allele frequency- A:0.02MN EU EA WA 534
    rs37326681,2
    C,F13586850(-) GGTCCA/GGGCCA 2 -- ut511Minor allele frequency- G:0.22EA 1280
    --
    rs117199211,2
    C13565346(+) TGAGGC/TGGCCG 2 -- ng510--------
    rs98199171,2
    H13563698(+) CAATCT/CGTCAT 2 -- ng514Minor allele frequency- C:0.00EU EA WA 420
    About this table

    HapMap Linkage Disequilibrium images for FBLN2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 135821

    2 Novoseek disease relationships for FBLN2 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    solar elastosis 70.92 3 11531782 (3)
    tumors 0.00 1 8245130 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/63 PubMed articles for FBLN2 gene (see all 63 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 2199 HGNC: 3601 AceView: FBLN2 Ensembl:ENSG00000163520 euGenes: HUgn2199
    ECgene: FBLN2 H-InvDB: FBLN2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for FBLN2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Millipore Immunoassay Services
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     Predesigned and custom siRNAs for FBLN2 Antibodies for FBLN2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for FBLN2 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
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     Antibodies for FBLN2

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