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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

FASN Gene

protein-coding GIFtS: 72
GCID: GC17M080037

fatty acid synthase

Explore 82 diseases affiliated with
FASN via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Fatty Acid Synthase¹ ²		Short Chain Dehydrogenase/Reductase Family 27X, Member 1²
FAS¹ ² ³		EC 2.3.1.85³
SDR27X1¹ ²		EC 2.3.1⁸
OA-519²		EC 6.3.3.1⁸

External Ids:	HGNC: 3594¹	Entrez Gene: 2194²	Ensembl: ENSG00000169710⁷	OMIM: 600212⁵	UniProtKB: P49327³

Export aliases for FASN gene to outside databases

Previous GC identifers: GC17M079459 GC17M9J0077 GC17M080572 GC17M077629 GC17M075436

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for FASN:

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of

palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some

cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the

N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: FAS_HUMAN, P49327

Function: Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and

NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein

Gene Wiki entry for FASN (Fatty acid synthase)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000017.10 NC_018928.1 NT_010663.15

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the FASN gene promoter:
PPAR-alpha PPAR-gamma1 PPAR-gamma2 ATF
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 4): FASN promoter sequence

Search SABiosciences Chromatin IP Primers for FASN

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat FASN

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q25 Ensembl cytogenetic band: 17q25.3 HGNC cytogenetic band: 17q25

FASN Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
FASN gene location

GeneLoc information about chromosome 17 GeneLoc Exon Structure

GeneLoc location for GC17M080037: view genomic region (about GC identifiers)

Start:	80,036,214 bp from pter	End:	80,056,208 bp from pter
Size:	19,995 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: FAS_HUMAN, P49327 (See protein sequence)

Recommended Name: Fatty acid synthase

Size: 2511 amino acids; 273427 Da

Subunit: Homodimer which is arranged in a head to tail fashion

Subcellular location: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I

to stage IV

Miscellaneous: The relatively low beta-ketoacyl synthase activity may be attributable to the low 4'-phosphopantetheine

content of the protein

Sequence caution: Sequence=AAB35516.1; Type=Erroneous initiation; Sequence=AAC50259.1; Type=Miscellaneous discrepancy;

Note=Several sequencing errors; Sequence=BAE06070.1; Type=Erroneous initiation;

6/7 PDB 3D structures from and Proteopedia for FASN (see all 7):

1XKT (3D)

2CG5 (3D)

2JFD (3D)

2JFK (3D)

2PX6 (3D)

3HHD (3D)

Secondary accessions: Q13479 Q16702 Q4LE83 Q6P4U5 Q6SS02 Q969R1 Q96C68 Q96IT0

Explore the universe of human proteins at neXtProt for FASN: NX_P49327

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P49327

4 DME Specific Peptides for FASN (P49327)

NSFGFGG

GGLGGFG

DTACSSS

YIEAHGTGT

FASN Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_004095.4

ENSEMBL proteins:

ENSP00000304592 ENSP00000462949

Reactome Protein details: P49327
Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
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	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein: FASN OriGene Protein Over-expression Lysate: FASN OriGene Custom Protein Services for FASN
	GenScript Custom Purified and Recombinant Proteins Services for FASN
	Novus Biologicals FASN Proteins Novus Biologicals FASN Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for FASN

Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005737	cytoplasm	IDA	--
GO:0005739	mitochondrion	IEA	--
GO:0005794	Golgi apparatus	IDA	--
GO:0005829	cytosol	TAS	--
GO:0005886	plasma membrane	IDA	--

FASN for ontologies About GeneDecksing

FASN Antibody Products:

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	OriGene Antibodies (see all 3): FASN OriGene Custom Antibody Services for FASN
	GenScript Custom Superior Antibodies Services for FASN
	Novus Biologicals FASN Antibodies
	Abcam antibodies for FASN
	Uscn Antibodies for FASN
	ThermoFisher Antibody for FASN

Assay Products for FASN:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
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	GenScript Custom Assay Services for FASN
	Cell Signaling Technology (CST) Sandwich ELISA Kits for FASN
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for FASN

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

FASN for domains About GeneDecksing

5/20 InterPro domains/families (see all 20):

IPR016035 Acyl_Trfase/lysoPLipase

IPR013149 ADH_C

IPR016038 Thiolase-like_subgr

IPR011032 GroES-like

IPR020843 PKS_ER

Graphical View of Domain Structure for InterPro Entry P49327

ProtoNet protein and cluster: P49327

5 Blocks protein families:

IPB000794 Beta-ketoacyl synthase

IPB001031 Thioesterase

IPB001227 Acyl transferase domain

IPB002198 Short-chain dehydrogenase/reductase SDR

IPB006162 Phosphopantetheine attachment site

UniProtKB/Swiss-Prot: FAS_HUMAN, P49327

Similarity: Contains 1 acyl carrier domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: FAS_HUMAN, P49327

Function: Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and

NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein

Catalytic activity: Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+)

Catalytic activity: Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]

Catalytic activity: Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein]

Catalytic activity: Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] +

CO(2) + [acyl-carrier-protein]

Catalytic activity: (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH

Catalytic activity: A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O

Catalytic activity: Acyl-[acyl-carrier-protein] + NADP(+) = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADPH

Catalytic activity: Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate

Genatlas biochemistry entry for FASN:

fatty acid ethyl ester synthase 1,NADPH dependent

Enzyme Numbers (IUBMB): EC 2.3.1.85¹ EC 6.3.3.1² EC 2.3.1²

miRNA Products:		miRTarBase miRNAs that target FASN: hsa-mir-146a (MIRT005581)

		OriGene 3'-UTR Clone: FASN Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat FASN
		8/16 QIAGEN miScript miRNA Assays for microRNAs that regulate FASN (see all 16):
		hsa-miR-607 hsa-miR-15a hsa-miR-1224-3p hsa-miR-1260b hsa-miR-103a hsa-miR-424 hsa-miR-3662 hsa-miR-195

		SwitchGear 3'UTR luciferase reporter plasmid: FASN 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for FASN (see all 7) OriGene shRNA RFP: FASN OriGene siRNA: FASN
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat FASN

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for FASN

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for FASN (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for FASN (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: FASN (NM_004104)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for FASN
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat FASN

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for FASN
		Search LifeMap BioReagents cell lines for FASN

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for FASN

Gene Ontology (GO): 5/18 molecular function terms (GO ID links to tree view) (see all 18): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000166	nucleotide binding	--	--
GO:0004312	fatty acid synthase activity	TAS	--
GO:0004313	[acyl-carrier-protein] S-acetyltransferase activity	IEA	--
GO:0004314	[acyl-carrier-protein] S-malonyltransferase activity	IEA	--
GO:0004315	3-oxoacyl-[acyl-carrier-protein] synthase activity	IEA	--

FASN for ontologies About GeneDecksing

2 GenomeRNAi human phenotypes for FASN:

Increased cell death in HCC-19

Synthetic lethal with c-Myc af

Animal Models:
Mouse knock-outs for FASN: Fasn^tm1.1Sem Fasn^tm1Schi
8 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Fasn):

adipose tissue	behavior/neurological	endocrine/exocrine gland	growth/size	homeostasis/metabolism
liver/biliary system	mortality/aging	nervous system

FASN for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/13 super-pathways (see all 13) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Saturated fatty acid biosynthesis

Saturated fatty acid biosynthesis	1.00	Transcriptional activation of FAS monomer gene by ChREBP:MLX	0.33
Saturated fatty acid biosynthesis	1.00	palmitate biosynthesis I (animals)	0.17
Fatty acid biosynthesis	0.50	fatty acid biosynthesis initiation II	0.17
ChREBP activates metabolic gene expression	0.38

Regulation of Cholesterol Biosynthesis by SREBP (SREBF)

	Regulation of Cholesterol Biosynthesis by SREBP (SREBF)	1.00			SREBP signalling	0.46
	Activation of Gene Expression by SREBP (SREBF)	0.67

Vitamin B5 (pantothenate) metabolism

	Vitamin B5 (pantothenate) metabolism	1.00			Metabolism of water-soluble vitamins and cofactors	0.21
	Metabolism of vitamins and cofactors	0.21

Metabolism

	Metabolism	1.00			Metabolism of lipids and lipoproteins	0.34
	Metabolic pathways	0.38

Fatty Acyl-CoA Biosynthesis

Fatty Acyl-CoA Biosynthesis

1.00

Triglyceride Biosynthesis

0.47

Pathway sources
See GeneCards unified pathways
Show all pathways

1 EMD Millipore Pathway for FASN

Saturated fatty acid biosynthesis

1 Downloadable PowerPoint Slide of QIAGEN Pathway Central Maps for FASN

AMPK Enzyme Complex Pathway

1 Cell Signaling Technology (CST) Pathway for FASN

Glucose / Energy Metabolism

1 GeneGo (Thomson Reuters) Pathway for FASN

Saturated fatty acid biosynthesis

5 BioSystems Pathways for FASN

	SREBP signalling
	AMPK signaling
	Fatty Acid Biosynthesis
	palmitate biosynthesis I (animals)
	fatty acid biosynthesis initiation II

5/14 Reactome Pathways for FASN (see all 14)

	Transcriptional activation of FAS monomer gene by ChREBP:MLX
	Expression of Fatty Acid Synthase (FASN)
	Metabolism
	Metabolism of vitamins and cofactors
	Triglyceride Biosynthesis

3 Kegg Pathways (Kegg details for FASN):

	Fatty acid biosynthesis
	Metabolic pathways
	Insulin signaling pathway

FASN for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for FASN

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/844 Interacting proteins for FASN (P49327^{2, 3} ENSP00000304592⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 844)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
HSP90AB1	P08238²^,³	MINT-7945693 MINT-72654 MINT-7947479 I2D: score=3
PRKDC	P78527²^,³, ENSP00000313420⁴	MINT-7945693 MINT-7947479 I2D: score=2 STRING: ENSP00000313420
MAP3K3	Q99759²^,³	MINT-48440 MINT-48461 I2D: score=2
NSF	P46459²^,³	MINT-7945693 MINT-7947479 I2D: score=1
PARP1	P09874²^,³, ENSP00000355759⁴	MINT-7947479 I2D: score=1 STRING: ENSP00000355759

About this table

Gene Ontology (GO): 5/12 biological process terms (GO ID links to tree view) (see all 12): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006084	acetyl-CoA metabolic process	IEA	--
GO:0006112	energy reserve metabolic process	TAS	--
GO:0006631	fatty acid metabolic process	TAS	7835891
GO:0006633	fatty acid biosynthetic process	IEA	--
GO:0006766	vitamin metabolic process	TAS	--

FASN for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

FASN for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Enzo Life Sciences drugs & compounds for FASN

Browse Tocris compounds for FASN

10/32 HMDB Compounds for FASN (see all 32) About this table

Compound	Synonyms	CAS #	PubMed Ids
(R)-3-Hydroxy-Octadecanoic acid		--	--
(R)-3-Hydroxy-hexadecanoic acid		--	--
(R)-3-Hydroxy-tetradecanoic acid		--	--
(R)-3-Hydroxydecanoic acid		--	--
(R)-3-Hydroxydodecanoic acid		--	--
(R)-3-Hydroxyoctanoic acid		--	--
3-Oxodecanoic acid		--	--
3-Oxododecanoic acid	3-Oxo-Dodecanoic acid (see all 4)	--	--
3-Oxohexadecanoic acid	3-Oxo-hexadecanoic acid (see all 4)	--	--
3-Oxotetradecanoic acid	3-Oxo-tetradecanoic acid (see all 4)	--	--

2 DrugBank Compounds for FASN About this table

Compound	Synonyms	CAS #	Type	Actions	PubMed Ids
Cerulenin	--	17397-89-6	target	inhibitor	17168665 11245456 12181752 11752352 20373869 10102370 15878185
Orlistat	(-)-Tetrahydrolipstatin (see all 4)	96829-58-2	target	inhibitor	16749788 15870086 16091048 11752352 15026345 15138278

10/105 Novoseek chemical compound relationships for FASN gene (see all 105) About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
cerulenin	94.6	37	15583825 (2), 9593836 (2), 9494573 (1), 17343199 (1) (see all 26)
fatty acid	94.3	1614	15920554 (9), 18639282 (7), 15751987 (6), 18555493 (6) (see all 99)
acetyl-coa	88.8	126	12213084 (4), 18760332 (2), 8944144 (2), 12759350 (2) (see all 87)
sterol	85.3	100	16603153 (2), 20067991 (2), 8605019 (2), 7592729 (2) (see all 64)
4'-phosphopantetheine	79	5	12815048 (1), 10206962 (1), 9405069 (1), 2318831 (1) (see all 5)
thiolactomycin	78.2	5	12926927 (2), 17242430 (1), 1510554 (1)
orlistat	75.2	15	18343106 (2), 17012255 (2), 18770866 (1), 16749788 (1) (see all 11)
palmitate	72.4	36	19782152 (2), 8022791 (2), 9485400 (2), 18796435 (2) (see all 21)
lipid	67.6	72	12646257 (2), 16048908 (1), 17413110 (1), 16788566 (1) (see all 53)
3-hydroxy-3-methylglutaryl-coa	67.3	19	8999918 (2), 8833906 (1), 11323427 (1), 12754279 (1) (see all 10)

Search CenterWatch for drugs/clinical trials and news about FASN / FAS

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for FASN gene:

NM_004104.4

Unigene Cluster for FASN:

Fatty acid synthase

Hs.83190 [show with all ESTs]

Unigene Representative Sequence: NM_004104

6 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000306749(uc002kdu.3 uc002kdv.1 uc002kdw.1) ENST00000584610

ENST00000580382 ENST00000579758 ENST00000578424 ENST00000579410

miRNA Products:		OriGene 3'-UTR Clone: FASN Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat FASN
		8/16 QIAGEN miScript miRNA Assays for microRNAs that regulate FASN (see all 16):
		hsa-miR-607 hsa-miR-15a hsa-miR-1224-3p hsa-miR-1260b hsa-miR-103a hsa-miR-424 hsa-miR-3662 hsa-miR-195

		SwitchGear 3'UTR luciferase reporter plasmid: FASN 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for FASN (see all 7) OriGene shRNA RFP: FASN OriGene siRNA: FASN
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat FASN

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for FASN (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for FASN (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: FASN (NM_004104)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for FASN
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat FASN

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for FASN Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat FASN
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat FASN
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat FASN

Additional cDNA sequence:

AB209988.1 AY451392.1 BC007267.1 BC007305.2 BC007909.1 BC014631.2 BC014634.2 BC021544.2

BC063242.1 S80437.1 U29344.1

24/32 DOTS entries (see all 32):

DT.100059743 DT.448160 DT.95110371 DT.100688445 DT.91871472 DT.100059746 DT.120978580 DT.120978656

DT.100648786 DT.100835114 DT.95110373 DT.120889547 DT.120978629 DT.120873367 DT.120978595 DT.120978641

DT.97821899 DT.100648788 DT.100773814 DT.120978591 DT.120978593 DT.75170060 DT.91780266 DT.95110370

24/885 AceView cDNA sequences (see all 885):

BG674267 BM470710 BQ891897 AA062768 BG115934 AI378876 AY451392 BE221247

BG117353 BQ366242 CR599966 CA406684 NM_004104 BU542310 BU850210 CB266237

BQ957144 BM480368 BQ958482 CA406132 BE254279 CA489676 BE563330 BU859013

GeneLoc Exon Structure

5/6 Alternative Splicing Database (ASD) splice patterns (SP) for FASN (see all 6) About this scheme

ExUns:	1	^	2a	·	2b	^	3	^	4	^	5	^	6	^	7a	·	7b	^	8	^	9	^	10	^	11	^	12	^	13	^	14	^	15	^	16	^	17	^	18	^	19	^	20	^	21a	·	21b	^	22	^	23	^
SP1:
SP2:
SP3:
SP4:
SP5:

ExUns:	24	^	25	^	26	^	27	^	28	^	29	^	30	^	31	^	32a	·	32b	^	33	^	34	^	35	^	36	^	37a	·	37b	·	37c	^	38	^	39a	·	39b	·	39c	^	40a	·	40b	·	40c	^	41	^	42	^
SP1:
SP2:																			-		-		-		-		-
SP3:
SP4:																																											-
SP5:																																	-		-		-		-		-		-		-

ExUns:	43a	·	43b	·	43c
SP1:
SP2:
SP3:
SP4:
SP5:

ECgene alternative splicing isoforms for FASN

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

FASN expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TGATCTCCAA

About this image

FASN expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

3 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Adipose

Body Subcutaneous White Adipose

Adipose

Visceral White Adipose

Adipose

Eye

Retina

Eye

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See FASN Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for FASN

SOURCE GeneReport for Unigene cluster: Hs.83190

UniProtKB/Swiss-Prot: FAS_HUMAN, P49327

Tissue specificity: Ubiquitous. Prominent expression in brain, lung, and liver

SABiosciences Expression via Pathway-Focused PCR Arrays including FASN (see all 8):

Insulin Signaling Pathway in human mouse rat

Fatty Acid Metabolism in human mouse rat

Insulin Resistance in human mouse rat

Prostate Cancer in human mouse rat

Hepatotoxicity in human mouse rat

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In Situ
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(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for FASN gene from 5/21 species (see all 21) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	FASN¹	fatty acid synthase	64.74(n) 66.44(a)		396061 NM_205155.2 NP_990486.2
lizard (Anolis carolinensis)	Reptilia	FASN⁶	--	65(a)	1 ↔ 1	2(118071990-118120473)
zebrafish (Danio rerio)	Actinopterygii	BI474038.1²	--	73.6(n)		BI474038.1
fruit fly (Drosophila melanogaster)	Insecta	BcDNA:GH07626³ CG3523¹	fatty-acid synthase³ CG3523¹	48(a) (best of 3)³ 55.64(n)¹ 47.82(a)¹		23D1³ 33524¹ NM_001144306.1¹ NP_001137778.1¹
worm (Caenorhabditis elegans)	Secernentea	F32H2.5³ fasn-1¹	fatty acid synthase³ Protein FASN-1¹	41(a) (best of 2)³ 49.44(n)¹ 43.92(a)¹		I(8984016-8992830)³ 172715¹ NM_060016.4¹ NP_492417.2¹

ENSEMBL Gene Tree for FASN (if available)
TreeFam Gene Tree for FASN (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for FASN gene

OXSM²

FASN for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 17 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs79381822^1,2	F,	--	80035748(+)	`CCAGCC/TTCCAC`	1	--	ds500¹		1		WA	118
rs17848956^1,2	C,F,	--	80035818(-)	`CACCAA/GGACGG`	1	--	ds500¹		8		NA WA EA	368
rs182718326^1,2	C,	--	80035895(+)	`TCCACG/AAGGGG`	1	--	ds500¹		1		EU	477
rs57295390^1,2	C	--	80035990(+)	`TACCCG/CCCCCC`	1	--	ds500¹		1		NA	2
rs15324^1,2	C,H	--	80036359(-)	`TTGTTT/CTTCAA`	1	--	ut3¹ ese³		6		MN NS EA NA	566
rs185555023^1,2		--	80036401(+)	`CCCGGC/TGCCGG`	1	--	ut3¹		0	--	--	--	--
rs189969201^1,2		--	80036495(+)	`TCCTCA/GGGGAC`	1	--	ut3¹		0	--	--	--	--
rs111482610^1,2		--	80036534(+)	`GAGCCA/CATGCC`	1	--	ut3¹		2		CSA WA	120
rs151047127^1,2		--	80036593(+)	`CGCAGA/GGGACA`	1	--	ut3¹		0	--	--	--	--
rs116196441^1,2	F,	--	80036636(+)	`ACGCTG/ACCTGA`	1	--	ut3¹		1		WA	118

HapMap Linkage Disequilibrium report for FASN (80036214 - 80056208 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for FASN
1 CNV: 73078
Human Gene Mutation Database (HGMD): FASN

SABiosciences Cancer Mutation PCR Assays

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

FASN for disorders           About GeneDecksing

OMIM gene information: 600212    OMIM disorders: --

20/82 diseases for FASN (see all 82):    About MalaCards

diffuse large b-cell lymphoma b-cell lymphomas traumatic brain injury trophoblastic neoplasm

brain injury glucose intolerance barrett's esophagus insulin resistance

granular cell tumor metabolic disorders oral squamous cell carcinoma fatty liver disease

squamous cell carcinoma breast cancer susceptibility pancreatic ductal adenocarcinoma inflammatory bowel disease

hypobetalipoproteinemia morbid obesity carcinoma hypertriglyceridemia

10/58 Novoseek disease relationships for FASN gene (see all 58) About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
fatty liver	60.1	12	14642615 (3), 19929925 (2), 10549164 (1), 9629539 (1) (see all 7)
obesity	56.9	35	19876008 (5), 15220220 (3), 19300427 (3), 16611078 (1) (see all 16)
cancer	53.7	209	17168665 (7), 15607569 (6), 10716717 (4), 15533929 (3) (see all 99)
microsatellite instability	52.2	6	19186181 (1), 19513025 (1), 19505918 (1), 19790197 (1) (see all 6)
prostate cancer	52.2	67	17477363 (5), 18774944 (4), 18817351 (3), 15050917 (3) (see all 34)
breast cancer	51.7	146	15870863 (7), 17786307 (6), 15583825 (5), 12531699 (5) (see all 54)
breast carcinoma	49.6	23	8630954 (3), 11447743 (2), 8022791 (1), 10228495 (1) (see all 14)
insulin resistance	46.3	6	18483661 (1), 19733654 (1), 19181734 (1)
tumors	46.1	131	9191002 (5), 18796435 (4), 10705076 (3), 15897909 (3) (see all 66)
carcinoma	37.7	27	15051923 (2), 20228782 (2), 9815604 (2), 11323104 (1) (see all 18)

Human Genome Epidemiology (HuGE) Navigator: FASN (17 documents)
Tumor Gene Database (TGDB): FASN

Export disorders for FASN gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for FASN gene, integrated from 9 sources (see all 736):
(articles sorted by number of sources associating them with FASN)

Utopia: connect your pdf to the dynamic
world of online information

Human fatty acid synthase: properties and molecular cloning. (PubMed id 7567999)^{1, 2, 3, 9} Jayakumar A.... Wakil S.J. (1995)
Human fatty acid synthase: structure and substrate selectivity of the thioesterase domain. (PubMed id 15507492)^{1, 2, 9} Chakravarty B.... Quiocho F.A. (2004)
Crystal structure of the thioesterase domain of human fatty acid synthase inhibited by Orlistat. (PubMed id 17618296)^{1, 2, 9} Pemble C.W. IV... Lowther W.T. (2007)
Human fatty acid synthase mRNA: tissue distribution, genetic mapping, and kinetics of decay after glucose deprivation. (PubMed id 7595075)^{1, 2, 9} Semenkovich C.F.... Fiedorek F.T. Jr. (1995)
Fatty acid synthesis: a potential selective target for antineoplastic therapy. (PubMed id 8022791)^{1, 2, 9} Kuhajda F.P.... Pasternack G.R. (1994)
The SDR (short-chain dehydrogenase/reductase and related enzymes) nomenclature initiative. (PubMed id 19027726)^{1, 3} Persson B.... Oppermann U. (2009)
Mechanism and substrate recognition of human holo ACP synthase. (PubMed id 18022563)^{1, 2} Bunkoczi G....Oppermann U. (2007)
Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes. (PubMed id 17081065)^{1, 2} Chi A....Hunt D.F. (2006)
A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)^{1, 2} Beausoleil S.A.... Gygi S.P. (2006)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 2194	HGNC: 3594	AceView: FASN	Ensembl:ENSG00000169710	euGenes: HUgn2194
ECgene: FASN	Kegg: 2194	H-InvDB: FASN

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for FASN	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for FASN	Genetics and Cytogenetics in Oncology and Haematology

Intellectual Property for FASN gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for FASN gene:
Search GeneIP for patents involving FASN

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
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fatty acid synthase

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FASN Gene

fatty acid synthase

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