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FAM65C Gene

protein-coding   GIFtS: 45
GCID: GC20M049202

Family With Sequence Similarity 65, Member C

(Previous names: chromosome 20 open reading frame 175, chromosome 20 open...)
(Previous symbols: C20orf175, C20orf176)
  Search for FAM65C
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Family With Sequence Similarity 65, Member C1 2     Chromosome 20 Open Reading Frame 1761
C20orf1751 2 3     dJ530I15.22
C20orf1761 2     dJ530I15.32
Chromosome 20 Open Reading Frame 1751     Protein FAM65C2

External Ids:    HGNC: 161681   Entrez Gene: 1408762   Ensembl: ENSG000000420627   UniProtKB: Q96MK23   

Export aliases for FAM65C gene to outside databases

Previous GC identifers: GC20M048637 GC20M045952


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for FAM65C Gene:
FAM65C (family with sequence similarity 65, member C) is a protein-coding gene. An important paralog of this gene is FAM65A.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000020.10  NT_011362.11  NC_018931.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the FAM65C gene promoter:
         LHX3b/Lhx3b   AP-1   NF-kappaB   CREB   FOXJ2 (long isoform)   deltaCREB   LHX3a/Lhx3a   FOXJ2   NF-kappaB1   Ik-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidFAM65C promoter sequence
   Search Chromatin IP Primers for FAM65C

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat FAM65C


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20q13.13   Ensembl cytogenetic band:  20q13.13   HGNC cytogenetic band: 20q13.13

FAM65C Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
FAM65C gene location

GeneLoc information about chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20M049202:  view genomic region     (about GC identifiers)

Start:
49,202,645 bp from pter      End:
49,308,065 bp from pter
Size:
105,421 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: FA65C_HUMAN, Q96MK2 (See protein sequence)
Recommended Name: Protein FAM65C  
Size: 946 amino acids; 105290 Da
Secondary accessions: Q5QPB6 Q9NQQ2
Alternative splicing: 2 isoforms:  Q96MK2-1   Q96MK2-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for FAM65C: NX_Q96MK2

Explore proteomics data for FAM65C at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See FAM65C Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001277197.1  NP_543019.2  

    ENSEMBL proteins: 
     ENSP00000332663   ENSP00000439802   ENSP00000045083  

    FAM65C Human Recombinant Protein Products:

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    1 InterPro protein domain:
     IPR026136 FAM65

    Graphical View of Domain Structure for InterPro Entry Q96MK2

    ProtoNet protein and cluster: Q96MK2

    UniProtKB/Swiss-Prot: FA65C_HUMAN, Q96MK2
    Similarity: Belongs to the FAM65 family


    Find genes that share domains with FAM65C           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005488binding ----
    GO:0008009chemokine activity ----
         
    Find genes that share ontologies with FAM65C           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for FAM65C:
     Synthetic lethal with Ras 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for FAM65C
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    miRNA
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    miRTarBase miRNAs that target FAM65C:
    hsa-mir-124-3p (MIRT022829), hsa-mir-335-5p (MIRT018301)

    Block miRNA regulation of human, mouse, rat FAM65C using miScript Target Protectors
    2 qRT-PCR Assays for microRNAs that regulate FAM65C:
    hsa-miR-205* hsa-miR-4276
    SwitchGear 3'UTR luciferase reporter plasmidFAM65C 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for FAM65C
    Predesigned siRNA for gene silencing in human, mouse, rat FAM65C

    Gene Editing
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    Browse Sino Biological Human cDNA Clones
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat FAM65C

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for FAM65C


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    mitochondrion3
    cytosol2
    nucleus1

    Gene Ontology (GO): 1 cellular component term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576extracellular region ----

    Find genes that share ontologies with FAM65C           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for FAM65C
    Interactions:

        Search GeneGlobe Interaction Network for FAM65C

    Gene Ontology (GO): 1 biological process term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006955immune response ----

    Find genes that share ontologies with FAM65C           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for FAM65C (FA65C)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for FAM65C gene (2 alternative transcripts): 
    NM_001290268.1  NM_080829.3  

    Unigene Cluster for FAM65C:

    Family with sequence similarity 65, member C
    Hs.372578  [show with all ESTs]
    Unigene Representative Sequence: NM_080829
    7 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000327979(uc010zyt.2) ENST00000462842 ENST00000488529 ENST00000482129
    ENST00000462493(uc002xvn.1) ENST00000535356(uc010zyu.1) ENST00000045083(uc002xvm.3)

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat FAM65C using miScript Target Protectors
    2 qRT-PCR Assays for microRNAs that regulate FAM65C:
    hsa-miR-205* hsa-miR-4276
    SwitchGear 3'UTR luciferase reporter plasmidFAM65C 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for FAM65C
    Predesigned siRNA for gene silencing in human, mouse, rat FAM65C
    Clone
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    OriGene ORF clones in mouse, rat for FAM65C
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: FAM65C (NM_080829)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for FAM65C
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat FAM65C
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for FAM65C
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat FAM65C
      QuantiTect SYBR Green Assays in human, mouse, rat FAM65C
      QuantiFast Probe-based Assays in human, mouse, rat FAM65C

    Additional mRNA sequence: 

    AK056792.1 AK124347.1 AK130652.1 AK295781.1 AK299337.1 BC131485.1 

    9 DOTS entries:

    DT.111567  DT.70104763  DT.99929314  DT.91916700  DT.100746700  DT.120811161  DT.97804580  DT.100644322 
    DT.97803433 

    Selected AceView cDNA sequences (see all 67):

    AA258384 AI151129 AI761954 AW445044 AW593763 AI279714 AW025449 CA389799 
    AA437074 AW057583 BQ446237 BX495517 AL042920 AI261200 AI206264 AW611735 
    AI499082 AK124347 AW193098 AW189031 AI631908 AK056792 AW589773 AI056877 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    FAM65C expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TCCTGAGATG
    FAM65C Expression
    About this image


    FAM65C expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 2) fully expand
     
     Ovary (Reproductive System)
             Pre-Granulosa Cells Primordial Follicle
     
     Neural Tube (Nervous System)
             Floor plate-like cells
    FAM65C Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    FAM65C Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.372578
        Custom PCR Arrays for FAM65C
    Primer
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for FAM65C

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for FAM65C gene from Selected species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Fam65c1 , 5 family with sequence similarity 65, member C1, 5 79.07(n)1
    76.65(a)1
      2 (88.07 cM)5
    695531  NM_001080708.21  NP_001074177.11 
     1679801645 
    chicken
    (Gallus gallus)
    Aves FAM65C1 family with sequence similarity 65, member C 59.96(n)
    57.27(a)
      428169  XM_004947270.1  XP_004947327.1 
    lizard
    (Anolis carolinensis)
    Reptilia FAM65C6
    family with sequence similarity 65, member C
    52(a)
    1 ↔ 1
    GL343226.1(2314446-2372767)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia fam65c1 family with sequence similarity 65, member C 61.83(n)
    56.79(a)
      100145316  XM_002933171.2  XP_002933217.2 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc:1130701 zgc:113070 52.05(n)
    42.12(a)
      503535  NM_001012499.2  NP_001012517.1 
    worm
    (Caenorhabditis elegans)
    Secernentea C27H2.26
    Protein C27H2.2, isoform a (C27H2.2) mRNA, complet...
    17(a)
    1 → many
    IV(13952715-13964001) WBGene00007782


    ENSEMBL Gene Tree for FAM65C (if available)
    TreeFam Gene Tree for FAM65C (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for FAM65C gene
    FAM65A2  FAM65B2  
    2 SIMAP similar genes for FAM65C using alignment to 2 protein entries:     FA65C_HUMAN (see all proteins):
    FLJ00360    FAM65B

    Find genes that share paralogs with FAM65C           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for FAM65C (see all 1412)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 20 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1410332201,2
    C,Funtested149247330(+) GCCCAC/TGACCC 2 M V mis11Minor allele frequency- T:0.00NA 4186
    rs1118933161,2
    C--49202351(+) AGTACC/TGGTCT 1 -- int11Minor allele frequency- T:0.50CSA 2
    rs61260461,2
    C,H--49202964(+) TCTTAG/ACAGTT 1 -- ut316Minor allele frequency- A:0.00NS EA NA 418
    rs1873380111,2
    --49202976(+) ATTGAC/GGAGAT 1 -- ut310--------
    rs1456872891,2
    C--49203006(+) CTGGG-/TTGA  
            
    TTGAT
    1 -- ut310--------
    rs38333141,2
    C--49203009(+) GATTG-/ATTG  
            
    CATTT
    1 -- ut311Minor allele frequency- ATTG:0.00NA 2
    rs1917176141,2
    --49203179(+) GCACTC/TGCAGC 1 -- ut310--------
    rs1817199481,2
    --49203262(+) GGTGAA/CACCTG 1 -- ut310--------
    rs1864653031,2
    --49203265(+) GACACC/TTGGCT 1 -- ut310--------
    rs37465641,2
    H--49203380(+) GCAGCG/AAGGCC 1 -- ut314Minor allele frequency- A:0.00NS EA 418

    HapMap Linkage Disequilibrium report for FAM65C (49202645 - 49308065 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 4 variations for FAM65C:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2667883CNV Deletion23128226
    esv1002485CNV Deletion20482838
    esv2722503CNV Deletion23290073
    nsv834001CNV Loss17160897

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing FAM65C
    DNA2.0 Custom Variant and Variant Library Synthesis for FAM65C

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    Find genes that share disorders with FAM65C           About GenesLikeMe

    Genetic Association Database (GAD): FAM65C
    Human Genome Epidemiology (HuGE) Navigator: FAM65C (1 document)

    Export disorders for FAM65C gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for FAM65C gene integrated from 10 sources:
    (articles sorted by number of sources associating them with FAM65C)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PubMed id 20877624)1, 4 Hendrickson S.L....O'Brien S.J. (PLoS ONE 2010)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    3. The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (Nature 2001)
    4. A transcript finishing initiative for closing gaps in the human transcriptome. (PubMed id 15197164)1 Sogayar M.C....Zanette D.L. (Genome Res. 2004)
    5. Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. (PubMed id 15146197)1 Brandenberger R.... Stanton L.W. (Nat. Biotechnol. 2004)
    6. Expressed sequence tag analysis of human RPE/choroid for the NEIBank project: over 6000 non-redundant transcripts, novel genes and splice variants. (PubMed id 12107410)1 Wistow G.... Peterson K. (Mol. Vis. 2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 140876 HGNC: 16168 AceView: C20orf175 Ensembl:ENSG00000042062 euGenes: HUgn140876
    ECgene: FAM65C H-InvDB: FAM65C

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for FAM65C Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for FAM65C gene:
    Search GeneIP for patents involving FAM65C

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

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