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FAAH2 Gene

protein-coding   GIFtS: 44

GC0XP057330
fatty acid amide hydrolase 2
(Previous name: amidase domain containing )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: AMDD)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
AMDD 2, 3
EC 3.5.1.n2 3
FAAH-2 1, 2
FLJ31204 1, 2
OTTHUMP00000023420 2
RP11-479E16 2
RP11-479E16.1 1
Descriptions
Amidase domain-containing protein 3
Anandamide amidohydrolase 2 3
Oleamide hydrolase 2 3
amidase domain containing 1, 2
fatty acid amide hydrolase 2 2
External Ids
HGNC: 264401
Entrez Gene: 1585842
UniProtKB: Q6GMR73
Ensembl: ENSG000001655917
Search outside databases for aliases for FAAH2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for FAAH2:
Fatty acid amide hydrolases, such as FAAH1 (FAAH; MIM 602935) and FAAH2, hydrolyze primary fatty
acid amide substrates (e.g., oleamide) and may play a role in fatty acid catabolism (Wei et al.,
2006 [PubMed 17015445]).[supplied by OMIM]

UniProtKB/Swiss-Prot: FAAH2_HUMAN, Q6GMR7
Function: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid,
anandamide and myristic amide to their corresponding acids, thereby serving to terminate the
signaling functions of these molecules. Hydrolyzes monounsaturated substrate anandamide
preferentially as compared to polyunsaturated substrates

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xp11.1   Ensembl cytogenetic band:  Xp11.1   HGNC cytogenetic band: Xp11.1

FAAH2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP057330:     (about GC identifiers)

Start:
57,329,864 bp from pter
End:
57,532,351 bp from pter
Size:
202,488 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000023.9  NT_011630.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: FAAH2_HUMAN, Q6GMR7 (See protein sequence)
Recommended Name: Fatty-acid amide hydrolase 2  
Size: 532 amino acids; 58304 Da
Subunit: Homodimer (By similarity)
Subcellular location: Membrane; Single-pass membrane protein (Potential)
Secondary accessions: Q86VT2 Q96N98

REFSEQ proteins: NP_777572.2  

ENSEMBL proteins: 
ENSP00000364035 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (FAAH2)
Human Recombinant Proteins from Abnova (FAAH2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
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Antibodies for FAAH2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for FAAH2
Browse R&D Systems for Antibodies
Antibodies from Abcam (FAAH2), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for FAAH2

Assays for FAAH2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

1 InterPro domain/family:
 IPR000120 Amidase_sig_enz


   GeneDecks  FAAH2 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q6GMR7

ProtoNet protein and cluster: Q6GMR7

1 Blocks protein family: IPB000120 Amidase

UniProtKB/Swiss-Prot: FAAH2_HUMAN, Q6GMR7
Similarity: Belongs to the amidase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (FAAH2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_174912

              Applied Biosystems Silencer® siRNAs for FAAH2

              Sigma-Aldrich siRNA and siRNA Panels for FAAH2  
                     Sigma-Aldrich shRNA for FAAH2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for FAAH2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_174912
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_174912
                                 untagged cDNA clone in CMV expression vector: NM_174912 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_174912

UniProtKB/Swiss-Prot: FAAH2_HUMAN, Q6GMR7
Function: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid,
anandamide and myristic amide to their corresponding acids, thereby serving to terminate the
signaling functions of these molecules. Hydrolyzes monounsaturated substrate anandamide
preferentially as compared to polyunsaturated substrates
Enzyme regulation: Inhibited by O-aryl carbamates and alpha-keto heterocytes
Enzyme Number (IUBMB): EC 3.5.1.n2 

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016787 hydrolase activity IEA--
GO:0016884 carbon-nitrogen ligase activity, with glutamine as amido-N-donor IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



4 Interacting proteins for FAAH2 (ENSP000003640353) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
DAGLAENSP000002572153STRING (score=.728)
DAGLBENSP000002970563STRING (score=.716)
ABHD4ENSP000002163273STRING (score=.714)
--ENSP000002583803STRING (score=.702)
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Compounds for FAAH2 available from Tocris Bioscience
CompoundAction CAS number
MAFPPotent, irreversible FAAH inhibitor[188404-10-6]
Arachidonyl serotoninDual FAAH inhibitor/TRPV1 antagonist[187947-37-1]
JNJ 1661010Selective, reversible FAAH inhibitor[681136-29-8]
PalmitoylisopropylamideInhibitor of FAAH[189939-61-5]
PF 750Selective FAAH inhibitor[959151-50-9]
About this table



(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (FAAH2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_174912

              Sigma-Aldrich siRNA and siRNA Panels for FAAH2  
                     Sigma-Aldrich shRNA for FAAH2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_174912  

REFSEQ mRNAs for FAAH2 gene: 

NM_174912.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_174912  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_174912
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_174912
                                 untagged cDNA clone in CMV expression vector: NM_174912 

Additional cDNA sequence: AF086253.1 

3 DOTS entries:

DT.101410  DT.95370388  DT.100755024 

24/40 AceView cDNA sequences (see all 40 ):

AA370259 BM714381 CA313962 AI367190 AI811051 W67536 BX487151 BE671210 
R02488 AF086253 BF448239 BU685932 BI771343 BE504565 BI765011 BM675184 
BX110322 BI769981 BX476543 BI763065 BC073922 NM_174912 AK055766 CB306775 

highest scoring ESTs for FAAH2:

AI248721 AI367190 AI457475 AI686695 AI811051 AK055766 BC048279 BF221899 BM675184 BM714381 

Unigene Clusters for FAAH2:

Fatty acid amide hydrolase 2
Hs.496205  [show with all ESTs], Hs.719735
Unigene Representative Sequences: NM_174912, AF086253


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000374900  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

FAAH2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for FAAH2

1 / 2 / 3

3 probe-sets matching FAAH2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

47313_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AI457475 0.80 1.00 0.91 1

230792_at2, 3 U133-B 1 1.00 1.00 -- -- BE671210 0.60 1.00 0.82 1

230792_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene clusters: Hs.496205 Hs.719735

UniProtKB/Swiss-Prot: FAAH2_HUMAN, Q6GMR7
Tissue specificity: Highly expressed in the brain, small intestine and testis. Also expressed in
the heart, kidney, liver, lung and prostate

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for FAAH2 gene from 5/6 species (see all 6 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
zebrafish
(Danio rerio)
Dr.168322   -- Transcribed sequence with weak similarity to protein more 73(n)  CF999295.1 
fruit fly
(Drosophila melanogaster)
CG88391   -- CG8839 53.01(n)
47.61(a)
36340  NM_165886.2  NP_725139.1 
worm
(Caenorhabditis elegans)
Y53F4B.181   -- hypothetical protein 47.6(n)
40.33(a)
190217  NM_064702.1  NP_497103.1 
African malaria mosquito
(Anopheles gambiae)
AgaP_AGAP0113151   -- AGAP011315-PA 51.38(n)
47.93(a)
1270620  XM_309335.2  XP_309335.2 
rice blast fungus
(Magnaporthe grisea)
MGG_027261   -- hypothetical protein 41.38(n)
28.51(a)
2682326  XM_366650.1  XP_366650.1 
About this table        Species with no ortholog for FAAH2

ENSEMBL Gene Tree for FAAH2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for FAAH2 gene
FAAH2  QRSL12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/721 NCBI SNPs in FAAH2 are shown (see all 721 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 153)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr X posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs10483581,2
A,C,F,H,O57532164(+) TGAAAC/TCAAGC 1 -- ut3112Minor allele frequency- T:0.45MN NA EA EU WA 1106
rs66127701,2
H57328484(+) TTCAAC/TGCTCA 1 -- ng514Minor allele frequency- T:0.00EU EA WA 418
rs25160231,2
A,C,F,H,O57330082(+) GGGGGC/TCCAAA 1 G/G syn1 ese38Minor allele frequency- T:0.49NA EU EA WA 636
rs13678301,2
A,C,F,H,O57491857(-) TTGAAA/GGGCCG 1 P/P syn1 ese314Minor allele frequency- G:0.44NA EA EU WA 1020
rs48265431,2
A,C,F,H57421888(+) TGCCGC/TTATGC 1 R/R syn18Minor allele frequency- T:0.13EU EA WA NA 650
rs9577211,2
A,C,F,H,O57332136(-) AAAAAT/AGTCAG 1 -- int110Minor allele frequency- A:0.50NA EA EU WA 812
rs24995551,2
A,C,F,H,O57335216(+) ccctaC/ACTTTA 1 -- int19Minor allele frequency- A:0.47EU EA WA NA 742
rs25160251,2
A,C,F,H,O57334430(+) TCATGT/CATTTG 1 -- int17Minor allele frequency- C:0.11EU EA WA NA 560
rs9807351,2
A,C,F,H,O57438033(+) GTCACG/ACATCA 1 -- int14Minor allele frequency- A:0.37EU EA WA 418
rs14316771,2
A,C,F,H,O57510179(-) AAAACC/AACAGC 1 -- int14Minor allele frequency- A:0.50EU EA WA 418
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HapMap Linkage Disequilibrium images for FAAH2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300654

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

7 PubMed articles for FAAH2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 158584 HGNC: 26440 AceView: FLJ31204 Ensembl:ENSG00000165591 euGenes: HUgn158584
ECgene: FAAH2 H-InvDB: FAAH2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for FAAH2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for FAAH2-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for FAAH2 Antibodies for FAAH2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for FAAH2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Search OriGene for FAAH2
 Tocris compounds for FAAH2




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Search Novus for antibodies for FAAH2

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