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F2RL2 Gene

protein-coding   GIFtS: 59

GC05M075947
coagulation factor II (thrombin) receptor-like 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
PAR-3 3
PAR3 1, 2, 3, 5
Descriptions
Coagulation factor II receptor-like 2 3
Coagulation factor II receptor-like 2 (protease-actovated
receptor 3) 2
Thrombin receptor-like 2 2, 3
coagulation factor II (thrombin) receptor-like 2 2
protease-activated receptor 3 2
proteinase-activated receptor-3 2
External Ids
HGNC: 35391
Entrez Gene: 21512
UniProtKB: O002543
Ensembl: ENSG000001642207
Search outside databases for aliases for F2RL2 gene

Previous GC identifers: GC05M074718 GC05M076147 GC05M075951 GC05M075995

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for F2RL2:
Coagulation factor II (thrombin) receptor-like 2 (F2RL2) is a member of the large family of
7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL2 is
also a member of the protease-activated receptor family and activated by thrombin. F2RL2 is
activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus
functions as a tethered ligand and activates the receptor. F2RL2 is a cofactor for F2RL3
activation by thrombin. It mediates thrombin-triggered phosphoinositide hydrolysis and is
expressed in a variety of tissues. [provided by RefSeq]

UniProtKB/Swiss-Prot: PAR3_HUMAN, O00254
Function: Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide
hydrolysis

Gene Wiki entry for F2RL2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the F2RL2 gene  

Entrez Gene cytogenetic band: 5q13   Ensembl cytogenetic band:  5q13.3   HGNC cytogenetic band: 5q13

F2RL2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M075947:     (about GC identifiers)

Start:
75,947,063 bp from pter
End:
75,954,996 bp from pter
Size:
7,934 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000005.8  NT_006713.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: PAR3_HUMAN, O00254 (See protein sequence)
Recommended Name: Proteinase-activated receptor 3 precursor  
Size: 374 amino acids; 42508 Da
Subunit: Interacts with INSC/inscuteable and probably GPSM2
Subcellular location: Cell membrane; Multi-pass membrane protein
Secondary accessions: Q52M68

Post-translational modifications:

  • A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand1


  • REFSEQ proteins: NP_004092.1  

    ENSEMBL proteins: 
    ENSP00000296641 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Proteinase Activated Receptor 3)
    Human Recombinant Proteins from Abnova (F2RL2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886 plasma membrane TAS9087410
    GO:0005887 integral to plasma membrane TAS9087410
    About this table

    Antibodies for F2RL2: 
    Invitrogen Antibodies for F2RL2
    Millipore Mono- and Polyclonal Antibodies for the study of F2RL2
    Sigma-Aldrich Antibodies for F2RL2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Proteinase Activated Receptor 3), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Novus Biologicals Antibody for F2RL2

    Assays for F2RL2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR017452 GPCR_Rhodpsn_supfam
     IPR003943 Prot_act_rcpt_3
     IPR003912 Protea_act_rcpt
     IPR000276 7TM_GPCR_Rhodpsn


       GeneDecks  F2RL2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O00254

    ProtoNet protein and cluster: O00254

    2 Blocks protein families:
    IPB003912 Protease activated receptor precursor signature
    IPB003943 Protease activated receptor 3 precursor signature


    UniProtKB/Swiss-Prot: PAR3_HUMAN, O00254
    Similarity: Belongs to the G-protein coupled receptor 1 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (F2RL2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (F2RL2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004101

                  Applied Biosystems Silencer® siRNAs for F2RL2

                  Sigma-Aldrich siRNA and siRNA Panels for F2RL2  
                         Sigma-Aldrich shRNA Panels and shRNA for F2RL2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for F2RL2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004101
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004101
                                     untagged cDNA clone in CMV expression vector: NM_004101 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_004101

    UniProtKB/Swiss-Prot: PAR3_HUMAN, O00254
    Function: Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide
    hydrolysis

    Genatlas biochemistry entry for F2RL2:
    coagulation factor II (thrombin) receptor-like 2,G protein coupled receptor superfamily

    2 MGI mutant phenotypes (inferred from 1 allele(MGI details for F2rl2):

    hematopoietic systemhomeostasis/metabolism

    5 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004435 phosphoinositide phospholipase C activity TAS9087410
    GO:0004872 receptor activity IEA--
    GO:0004930 G-protein coupled receptor activity IEA--
    GO:0005515 protein binding IPI17474147
    GO:0015057 thrombin receptor activity TAS9087410
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for F2RL2
     Neuropeptides signaling through G-protein alpha-i & G-protein alpha-q

       GeneDecks  F2RL2 for the pathways selected above  
    About GeneDecksing

    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  F2RL2  (Your Favorite Gene powered by Ingenuity) 
     Thrombin Signaling
     Tight Junction Signaling

       GeneDecks  F2RL2 for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for F2RL2):
     hsa04080 Neuroactive ligand-receptor interaction

       GeneDecks  F2RL2 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  F2RL2 


    5/8 Interacting proteins for F2RL2 (O002541, 2) via UniProtKB, MINT, and/or STRING (see all 8 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ABL1P005191EBI-1751853, EBI-375543
    CRKP461081EBI-1751853, EBI-886
    FYNP062411EBI-1751853, EBI-515315
    GRB2P629931EBI-1751853, EBI-401755
    NCK1P163331EBI-1751853, EBI-389883
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007165 signal transduction IEA--
    GO:0007186 G-protein coupled receptor protein signaling pathway IEA--
    GO:0007596 blood coagulation TAS9087410
    GO:0009611 response to wounding TAS9087410
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for F2RL2
    Browse Small Molecules at Sigma-Aldrich

    Compounds for F2RL2 available from Tocris Bioscience
    8 Novoseek chemical compound relationships for F2RL2 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    bcma 66.45 2 17968879 (1), 14613291 (1)
    calcium 24.44 5 15317288 (1), 18264801 (1), 14515192 (1), 10831593 (1)
    rantes 23.02 1 12176812 (1)
    tyrosine 19.03 4 2019259 (1), 7798258 (1), 17047157 (1), 16525676 (1)
    chloride 8.01 1 16623543 (1)
    glucose 3.57 2 17362009 (1)
    phosphoinositide 1.09 1 9087410 (1)
    fibrinogen 0.00 2 9765662 (1), 12732212 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (F2RL2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (F2RL2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004101

                  Sigma-Aldrich siRNA and siRNA Panels for F2RL2  
                         Sigma-Aldrich shRNA Panels and shRNA for F2RL2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_004101  

    REFSEQ mRNAs for F2RL2 gene: 

    NM_004101.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_004101  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004101
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004101
                                     untagged cDNA clone in CMV expression vector: NM_004101 

    Additional cDNA sequence: 

    AK298585.1 AK312848.1 BC093648.1 BC093650.1 BC095499.1 BX537386.1 U92971.1 

    4 DOTS entries:

    DT.87015130  DT.443472  DT.120839145  DT.95265413 

    24/40 AceView cDNA sequences (see all 40 ):

    NM_004101 AA983803 U92971 AL048675 CF126741 H98544 AA570482 N26732 
    AW973423 AI131231 H98551 BX537386 BX474658 AI378647 BX102060 AL713459 
    BM989640 CN834303 H98537 H98520 AA483486 CN836476 AA041482 N34619 

    highest scoring ESTs for F2RL2:

    U92971 AI378647 AL713459 BX474658 BX537386 CN833866 CN834303 CN836476 CN837623 AA041482 

    Unigene Cluster for F2RL2:

    Coagulation factor II (thrombin) receptor-like 2
    Hs.42502  [show with all ESTs]
    Unigene Representative Sequence: NM_004101


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000296641  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    F2RL2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for F2RL2

    1 / 2 / 3

    7 probe-sets matching F2RL2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    31972_at2, 3 U95-A 1 1.00 1.00 0.24 0.26 U92971 1.00 1.00 1.00 1

    45204_s_at2, 3 U95-B 1 1.00 1.00 0.93 0.96 AI378647 0.80 1.00 0.91 1

    62120_at2, 3 U95-C 3 1.00 0.96 0.63 1.05 AA483853 0.60 1.00 0.82 1

    206795_at2, 3 U133-A 1 1.00 1.00 -- -- NM_004101 0.60 1.00 0.82 1

    230147_at2, 3 U133-B 1 0.91 1.00 -- -- AI378647 0.80 1.00 0.91 1

    206795_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    230147_at2 U133Plus2 1 0.91 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AGCCACTACG

    SOURCE GeneReport for Unigene cluster: Hs.42502

    Expression variation in blood from EXPOLDB for F2RL2

    UniProtKB/Swiss-Prot: PAR3_HUMAN, O00254
    Tissue specificity: Highest expression in the megakaryocytes of the bone marrow, lower in mature
    megakaryocytes, in platelets and in a variety of other tissues such as heart and gut

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for F2RL2 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    F2RL21   -- coagulation factor II (thrombin) receptor-like 2 88.74(n)
    87.94(a)
    607963  XM_844773.1  XP_849866.1 
    cow
    (Bos taurus)
    F2RL21   -- coagulation factor II (thrombin) receptor-like 2 87.72(n)
    86.56(a)
    512581  NM_001038533.1  NP_001033622.1 
    rat
    (Rattus norvegicus)
    F2rl21   -- coagulation factor II (thrombin) receptor-like 2 76.32(n)
    71.58(a)
    29636  NM_053313.1  NP_445765.1 
    mouse
    (Mus musculus)
    F2rl21, 5 13 (47.02 cM)5
    coagulation factor II (thrombin) receptor-like 21, 5 77.16(n)1
    71.55(a)1
    140641  NM_010170.31  NP_034300.21 
     AK0894445  AK1352125  (see all 7)
    chicken
    (Gallus gallus)
    F2RL21   -- coagulation factor II (thrombin) receptor-like 2 65.29(n)
    62.64(a)
    768449  XM_001231245.1  XP_001231246.1 
    About this table        Species with no ortholog for F2RL2

    ENSEMBL Gene Tree for F2RL2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for F2RL2 gene
    P2RY82  GPR182  F2RL12  F2RL32  F2R2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section

      --
    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 601919

    10/12 Novoseek disease relationships for F2RL2 gene (see all 12 )

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    achondroplasia 50.01 2 12361605 (1), 15863034 (1)
    inflammation 31.00 2 17376866 (1)
    dwarfism 28.78 2 12361605 (1), 15863034 (1)
    erythroleukemia 19.50 3 15504554 (2)
    necrosis 6.92 7 14623878 (1), 15491417 (1), 9614115 (1), 16965421 (1)
    cancer 0.00 2 17114345 (1), 17164762 (1)
    carcinoma 0.00 3 11743661 (1), 15582715 (1)
    tumors 0.00 5 18623079 (1), 14623878 (1), 9614115 (1), 17308070 (1)
    hepatocellular carcinoma 0.00 1 17323377 (1)
    adenocarcinoma 0.00 1 15800658 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/158 PubMed articles for F2RL2 gene (see all 158 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 2151 HGNC: 3539 AceView: F2RL2 Ensembl:ENSG00000164220 euGenes: HUgn2151
    ECgene: F2RL2 H-InvDB: F2RL2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    SeattleSNPshttp://pga.gs.washington.edu/data/f2rl2/
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for F2RL2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for F2RL2 Antibodies for F2RL2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for F2RL2 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
    (Proteinase Activated Receptor 3)
     Antibodies (Proteinase Activated Receptor 3)
     Search OriGene for F2RL2
     Tocris compounds for F2RL2




     Drugs for F2RL2
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for F2RL2

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