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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

EVX2 Gene

protein-coding   GIFtS: 41
GCID: GC02M176908

even-skipped homeobox 2

(Previous names: eve, even-skipped homeobox homolog 2 (Drosophila) )
 Explore 5 diseases affiliated with
EVX2 via our new
 Human Malady Compendium 
Biological research products
for EVX2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Even-Skipped Homeobox 21 2
EVX-22 3
Eve, Even-Skipped Homeobox Homolog 2 (Drosophila)1
Eve, Even-Skipped Homeo Box Homolog 22
Even-Skipped Homeo Box 2 (Homolog Of Drosophila Eve)2
Homeobox Even-Skipped Homolog Protein 22

External Ids:    HGNC: 35071   Entrez Gene: 3441912   Ensembl: ENSG000001742797   OMIM: 1429915   UniProtKB: Q038283   

Export aliases for EVX2 gene to outside databases

Previous GC identifers: GC02U990026 GC02M175605 GC02M177147 GC02M176770 GC02M176653 GC02M168822


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for EVX2:
This gene is located at the 5' end of the HOXD gene cluster on chromosome 2. The encoded protein is a homeobox
transcription factor that is related to the protein encoded by the Drosophila even-skipped (eve) gene, a member of the
pair-rule class of segmentation genes. A 117 kb microdeletion at the 5' end of the HOXD gene cluster, which includes
this gene and the HOXD9-HOXD13 genes, causes synpolydactyly, a dominantly inherited disease resulting in limb
malformation. (provided by RefSeq, Sep 2009)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000002.11  NC_018913.1  NT_005403.17  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the EVX2 gene promoter:
         TBP   PPAR-gamma1   AP-1   ATF-2   c-Jun   PPAR-gamma2   
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search SABiosciences Chromatin IP Primers for EVX2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat EVX2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2q31.1   Ensembl cytogenetic band:  2q31.1   HGNC cytogenetic band: 2q31.1

EVX2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
EVX2 gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M176908:  view genomic region     (about GC identifiers)

Start:
176,942,200 bp from pter      End:
176,948,690 bp from pter
Size:
6,491 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: EVX2_HUMAN, Q03828 (See protein sequence)
Recommended Name: Homeobox even-skipped homolog protein 2  
Size: 476 amino acids; 47800 Da
Subcellular location: Nucleus
Developmental stage: Expressed during early embryogenesis and neurogenesis in a biphasic manner

Explore the universe of human proteins at neXtProt for EVX2: NX_Q03828

EVX2 Protein expression data from MOPED and PaxDb:    About this image 

Estimated protein expression log10 (pmol).

REFSEQ proteins: NP_001073927.1  
ENSEMBL proteins: 
 ENSP00000312385  

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Uscn Proteins for EVX2

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005634nucleus NAS--
GO:0005730NOT nucleolus IDA--


EVX2 for ontologies           About GeneDecksing



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(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

EVX2 for domains           About GeneDecksing

4 InterPro domains/families:
 IPR020479 Homeobox_metazoa
 IPR017970 Homeobox_CS
 IPR001356 Homeodomain
 IPR009057 Homeodomain-like

Graphical View of Domain Structure for InterPro Entry Q03828

ProtoNet protein and cluster: Q03828

1 Blocks protein family: IPB000047 Lambda and other repressor helix-turn-helix signature

UniProtKB/Swiss-Prot: EVX2_HUMAN, Q03828
Similarity: Belongs to the even-skipped homeobox family
Similarity: Contains 1 homeobox DNA-binding domain


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:
     Genatlas biochemistry entry for EVX2:
Drosophila even-skipped pair rule gene homolog 2 (murine Evx2),homeo domain encoding gene,expressed in limbs

miRNA
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Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0003677DNA binding NAS--
GO:0003700sequence-specific DNA binding transcription factor activity IEA--
GO:0043565sequence-specific DNA binding IEA--


EVX2 for ontologies           About GeneDecksing


Animal Models:
     Mouse knock-outs for EVX2: Evx2/Hoxd13Evx2/tm3.1Ddu Evx2tm1Ddu Evx2/Hoxd13Evx2/tm2Ddu Evx2/Hoxd11/Hoxd12/Hoxd13Evx2/Hoxd11/Hoxd12/tm1Ddu Evx2/Hoxd13Evx2/tm3Ddu Evx2/Hoxd13Evx2/tm1Ddu
     8 MGI mutant phenotypes (inferred from 9 alleles(MGI details for Evx2):
 embryogenesis  growth/size  limbs/digits/tail  mortality/aging  nervous system 
 no phenotypic analysis  reproductive system  skeleton 

EVX2 for phenotypes           About GeneDecksing


(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section



Interactions:

    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for EVX2

Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0008150biological_process ND--
GO:0035108limb morphogenesis IEA--


EVX2 for ontologies           About GeneDecksing



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section
Browse Small Molecules at EMD Millipore
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for EVX2
Search CenterWatch for drugs/clinical trials and news about EVX2 

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
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Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for EVX2 gene: 
NM_001080458.1  

Unigene Cluster for EVX2:

Even-skipped homeobox 2
Hs.452341  [show with all ESTs]
Unigene Representative Sequence: NM_001080458
1 Ensembl transcript including schematic representation, and UCSC links where relevant:
ENST00000308618(uc010zeu.2)

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Browse SwitchGear 3'UTR luciferase reporter plasmids
Inhib. RNA
Products:
     
Browse for Gene Knock-down Tools from EMD Millipore
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OriGene shRNA RFP: EVX2
OriGene siRNA: EVX2
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat EVX2
Clone
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OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for EVX2 (see all 3)
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GenScript: all cDNA clones in your preferred vector: EVX2 (NM_001080458)
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Primer
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  QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat EVX2
  QIAGEN QuantiFast Probe-based Assays in human, mouse, rat EVX2

1 DOTS entry:

DT.91921632 

1 AceView cDNA sequence:

BF057568 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

EVX2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: ATGACGCACG

Microarray
RNAseq (Illumina Body Map)
(100×FPKM)½
SAGE (Serial Analysis of Gene Expression)

About this image

EVX2 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
1 LifeMap In Vivo Development Anatomical Compartment/Cell 
Tissue Anatomical Compartment CellCategory (developmental path)
Spinal CordV0 Neural Progenitor DomainV0 InterneuronsMotor Neurons
Expression: Positive    Negative     Selective marker
Experimental details: Curated     Microarrays     In-situ hybridization

See EVX2 Protein Expression from SPIRE MOPED and PaxDB
SOURCE GeneReport for Unigene cluster: Hs.452341
    SABiosciences Custom PCR Arrays for EVX2
Primer
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QIAGEN QuantiFast Probe-based Assays in human, mouse, rat EVX2
In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EVX2

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for EVX2 gene from 6/16 species (see all 16)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Evx21 , 5 even skipped homeotic gene 2 homolog1, 5 88.35(n)1
95.13(a)1
  2 (44.13 cM)5
140291  NM_007967.21  NP_031993.11 
 746529975 
chicken
(Gallus gallus)
Aves EVX26
Uncharacterized protein
49(a)
1 ↔ 1
7(17444164-17447372)
lizard
(Anolis carolinensis)
Reptilia EVX26
--
61(a)
1 ↔ 1
GL343356.1(628378-632437)
zebrafish
(Danio rerio)
Actinopterygii evx22 even-skipped homeobox 2 76.28(n)   30479  NM_131232.1 
fruit fly
(Drosophila melanogaster)
Insecta eve3 blastoderm segmentation specific RNA
polymerase more
71(a)   46C10   --
worm
(Caenorhabditis elegans)
Secernentea vab-76
Homeobox protein vab-7
33(a)
1 → many
III(10920237-10925942)


ENSEMBL Gene Tree for EVX2 (if available)
TreeFam Gene Tree for EVX2 (if available) 

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
Paralogs for EVX2 gene
NKX1-22  MEOX12  EVX12  MNX12  MEOX22  NKX1-12  GBX12  GBX22  
1 SIMAP similar gene for EVX2 using alignment to 1 protein entry:     EVX2_HUMAN:
EVX1

EVX2 for paralogs           About GeneDecksing



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

10/97 NCBI SNPs in EVX2 are shown (see all 97    About this table
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 2 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs8471421,2
F,--168822182(-) CCGAAG/AGGAAC 1 -- ds50011Minor allele frequency- A:0.07WA 118
rs789382161,2
F,--168822253(+) CTCAGG/CGGGCG 1 -- ds50011Minor allele frequency- C:0.06WA 118
rs730299211,2
F,--168822264(+) CACAGG/AGGACT 1 -- ds50011Minor allele frequency- A:0.14WA 118
rs18681011,2
A,--168822498(-) ggcggC/Tggtgg 2 G syn1 ese30--------
rs762655811,2
--168823535(+) GGGTCC/TGATTT 1 -- int10--------
rs729190881,2
C,F,--168823664(+) TCATTC/TGTCAC 1 -- int11Minor allele frequency- T:0.04NA 120
rs1116248001,2
C,--168824309(+) AGCCAC/TCCAGA 1 -- int12Minor allele frequency- T:0.08CSA WA 120
rs1136584231,2
F--168824310(+) GCCACC/TCAGAA 1 -- int11Minor allele frequency- T:0.50CSA 4
rs730299241,2
C,F,--168824316(+) CAGAAA/GGGGGA 1 -- int13Minor allele frequency- G:0.10WA CSA 122
rs729190901,2
F,--168824755(+) GTCAGG/CAAGGA 1 -- int11Minor allele frequency- C:0.07NA 120

HapMap Linkage Disequilibrium report for EVX2 (176942200 - 176948690 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
      Database of Genomic Variants (DGV) variations for EVX2: --

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

EVX2 for disorders           About GeneDecksing

OMIM gene information: 142991    OMIM disorders: --

5 diseases for EVX2:    About MalaCards
synpolydactyly    sex reversal    currarino syndrome    syndactyly
neuronitis

1 disease from the University of Copenhagen DISEASES database for EVX2:
Syndactyly

Export disorders for EVX2 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
About This Section

PubMed articles for EVX2 gene integrated from 9 sources:
(articles sorted by number of sources associating them with EVX2)
    Utopia: connect your pdf to the dynamic
world of online information

  1. EVX2, a human homeobox gene homologous to the even-skipped segmentation gene, is localized at the 5' end of HOX4 locus on chromosome 2. (PubMed id 1675198)1, 2, 3 D'Esposito M....Boncinelli E. (1991)
  2. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)1, 2 Hillier L.W....Wilson R.K. (2005)
  3. Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
  4. A 117-kb microdeletion removing HOXD9-HOXD13 and EVX2 causes synpolydactyly. (PubMed id 11778160)1 Goodman F.R....Scambler P.J. (2002)
  5. NotI flanking sequences: a tool for gene discovery and verification of the human genome. (PubMed id 12136098)1 Kutsenko A.S....Zabarovsky E.R. (2002)
  6. The sequence of the human genome. (PubMed id 11181995)1 Venter J.C.... Zhu X. (2001)
  7. Clustering of two fragile sites and seven homeobox genes in human chromosome region 2q31-->q32.1. (PubMed id 11060466)1 Limongi M.Z....Rocchi A. (2000)
  8. Two cases with interstitial deletions of chromosome 2 and sex reversal in one. (PubMed id 10440834)1 Slavotinek A....Kingston H. (1999)
  9. Localization of the syndactyly type II (synpolydactyly) locus to 2q31 region and identification of tight linkage to HOXD8 intragenic marker. (PubMed id 7581388)1 Sarfarazi M....Sayli B.S. (1995)
  10. Order of six loci at 2q24-q31 and orientation of the HOXD locus. (PubMed id 7896287)1 Rossi E....Zuffardi O. (1994)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 344191 HGNC: 3507 AceView: LOC344191 Ensembl:ENSG00000174279 euGenes: HUgn344191
ECgene: EVX2 H-InvDB: EVX2

(According to HUGE)
About This Section
  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
NameDescription
PharmGKB entry for EVX2 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
About This Section
Patent Information for EVX2 gene:
Search GeneIP for patents involving EVX2

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section

 
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 QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat EVX2
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 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat EVX2
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 GenScript Custom Purified and Recombinant Proteins Services for EVX2 GenScript cDNA clones with any tag delivered in your preferred vector for EVX2
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 Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat EVX2
       
GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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VWF
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