Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 
or upload a file of gene symbols


Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

EPS15 Gene

protein-coding   GIFtS: 64
GCID: GC01M051819

Epidermal Growth Factor Receptor Pathway Substrate 15

Pneumococci & Pneumococcal Diseases Congress
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Epidermal Growth Factor Receptor Pathway Substrate 151 2     MLLT52
Protein AF-1p2 3     ALL1 Fused Gene From Chromosome 12
AF1P2 3     Epidermal Growth Factor Receptor Substrate 152
AF-1P2     Protein Eps153

External Ids:    HGNC: 34191   Entrez Gene: 20602   Ensembl: ENSG000000858327   OMIM: 6000515   UniProtKB: P425663   

Export aliases for EPS15 gene to outside databases

Previous GC identifers: GC01M051447 GC01M050709 GC01M051179 GC01M051190 GC01M051532 GC01M049937


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for EPS15 Gene:
This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and
is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene
in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct
isoforms. (provided by RefSeq, May 2009)

GeneCards Summary for EPS15 Gene: 
EPS15 (epidermal growth factor receptor pathway substrate 15) is a protein-coding gene. Diseases associated with EPS15 include troyer syndrome, and menkes disease, and among its related super-pathways are Retrograde neurotrophin signalling and Signaling by FGFR. GO annotations related to this gene include SH3 domain binding and calcium ion binding. An important paralog of this gene is REPS1.

UniProtKB/Swiss-Prot: EPS15_HUMAN, P42566
Function: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control
of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine
kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Seems to be
involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1)
and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require
association with DAB2

Gene Wiki entry for EPS15 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000001.10  NT_032977.9  NC_018912.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the EPS15 gene promoter:
         AP-1   AP-2alpha isoform 3   ATF-2   AP-2alpha isoform 2   E2F-1   E2F   AP-2alpha isoform 4   c-Jun   AP-2alpha   AP-2alphaA   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidEPS15 promoter sequence
   Search SABiosciences Chromatin IP Primers for EPS15

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat EPS15


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p32   Ensembl cytogenetic band:  1p32.3   HGNC cytogenetic band: 1p32

EPS15 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
EPS15 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M051819:  view genomic region     (about GC identifiers)

Start:
51,819,935 bp from pter      End:
51,985,000 bp from pter
Size:
165,066 bases      Orientation:
minus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: EPS15_HUMAN, P42566 (See protein sequence)
Recommended Name: Epidermal growth factor receptor substrate 15  
Size: 896 amino acids; 98656 Da
Subunit: Interacts with SGIP1 (By similarity). Interacts with HGS; the interaction bridges the interaction of STAM
or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS,
and either STAM1 or STAM2. Binds AP2A2. Interacts with AP2B1; clathrin competes with EPS15. Binds STON2 and EPN1.
Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP. Interacts with ERBB2. Interacts with
FCHO1. Interacts with FCHO2. Interacts (via EH domains) with DAB2
Subcellular location: Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane,
clathrin-coated pit. Note=Recruited to the plasma membrane upon EGFR activation and localizes to coated pits
Subcellular location: Isoform 2: Early endosome membrane; Peripheral membrane protein; Cytoplasmic side.
Note=Colocalizes with HGS on bilayered clathrin coats on endosomes
Caution: Studies in clathrin-mediated endocytosis of ITGB1 and TFR used a siRNA mixture of EPS15 and EPS15L1, and
a Dab2 mutant with impaired binding to EH domain-containing proteins EPS15 and ITSN1 suggesting a partially
overlapping role of the EH domain-containing proteins (PubMed:22648170)
6 PDB 3D structures from and Proteopedia for EPS15:
1C07 (3D)        1EH2 (3D)        1F8H (3D)        1FF1 (3D)        2IV9 (3D)        2JXC (3D)    
Secondary accessions: B2R8J7 D3DPJ2 Q5SRH4
Alternative splicing: 2 isoforms:  P42566-1   P42566-2   

Explore the universe of human proteins at neXtProt for EPS15: NX_P42566

Explore proteomics data for EPS15 at MOPED 

Post-translational modifications:

  • UniProtKB: Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation
    after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By
    similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR
  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_P42566

  • EPS15 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    EPS15 Protein Expression
    REFSEQ proteins (2 alternative transcripts): 
    NP_001153441.1  NP_001972.1  

    ENSEMBL proteins: 
     ENSP00000360795   ENSP00000360798   ENSP00000474739   ENSP00000379428  
    Reactome Protein details: P42566
    Human Recombinant Protein Products for EPS15: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for EPS15
    OriGene Protein Over-expression Lysate for EPS15
    OriGene MassSpec for EPS15 
    OriGene Custom Protein Services for EPS15
    GenScript Custom Purified and Recombinant Proteins Services for EPS15
    Novus Biologicals EPS15 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates 
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for EPS15 

    Gene Ontology (GO): 5/10 cellular component terms (GO ID links to tree view) (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA--
    GO:0005829cytosol TAS--
    GO:0005886plasma membrane IDA15465819
    GO:0005905coated pit TAS9762922
    GO:0009318exodeoxyribonuclease VII complex ----

    EPS15 for ontologies           About GeneDecksing



    EPS15 Antibody Products: 
    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    Cell Signaling Technology (CST) Antibodies for EPS15 
    OriGene Antibodies for EPS15
    OriGene Custom Antibody Services for EPS15
    GenScript Custom Superior Antibodies Services for EPS15
    Novus Biologicals EPS15 Antibodies
    Abcam antibodies for EPS15
    Cloud-Clone Corp. Antibodies for EPS15 
    ThermoFisher Antibodies for EPS15
    LSBio Antibodies in human, mouse, rat for EPS15 

    Assay Products for EPS15: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for EPS15
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for EPS15
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for EPS15 
    Cloud-Clone Corp. CLIAs for EPS15


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HGNC Gene Families: 
    EFHAND: EF-hand domain containing

    5 InterPro protein domains:
     IPR011992 EF-hand-like_dom
     IPR002048 EF_hand_dom
     IPR000261 EPS15_homology
     IPR003903 Ubiquitin-int_motif
     IPR018247 EF_Hand_1_Ca_BS

    Graphical View of Domain Structure for InterPro Entry P42566

    ProtoNet protein and cluster: P42566

    2 Blocks protein domains:
    IPB000261 EPS15 homology (EH)
    IPB003903 Ubiquitin interacting motif


    UniProtKB/Swiss-Prot: EPS15_HUMAN, P42566
    Domain: The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins
    Similarity: Contains 2 EF-hand domains
    Similarity: Contains 3 EH domains
    Similarity: Contains 2 UIM (ubiquitin-interacting motif) repeats


    EPS15 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: EPS15_HUMAN, P42566
    Function: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control
    of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine
    kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Seems to be
    involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1)
    and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require
    association with DAB2

         Genatlas biochemistry entry for EPS15:
    EGFR pathway substrate,mouse eps15 homolog,likely involved in cell growth regulation

         Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005509calcium ion binding IEA--
    GO:0005515protein binding IPI10383425
    GO:0008855exodeoxyribonuclease VII activity ----
    GO:0017124SH3 domain binding IEA--
         
    EPS15 for ontologies           About GeneDecksing


    Phenotypes:
         4 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Eps15):
     cellular  hematopoietic system  immune system  nervous system 

    EPS15 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Eps15tm1Noff for EPS15

       inGenious Targeting Laboratory - Custom generated mouse model solutions for EPS15 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for EPS15

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for EPS15 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for EPS15 

    miRNA
    Products:
        
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat EPS15
    8/78 QIAGEN miScript miRNA Assays for microRNAs that regulate EPS15 (see all 78):
    hsa-miR-607 hsa-miR-374a hsa-miR-138-2* hsa-miR-371-5p hsa-miR-556-3p hsa-miR-200b hsa-miR-130b hsa-miR-130a*
    SwitchGear 3'UTR luciferase reporter plasmidEPS15 3' UTR sequence
    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for EPS15
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat EPS15

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for EPS15
    Sirion Biotech Customized adenovirus for overexpression of EPS15

    Clone
    Products:
         
    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for EPS15 (see all 12)
    OriGene ORF clones in mouse, rat for EPS15
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): EPS15 (NM_001981)
    Sino Biological Human cDNA Clone for EPS15
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for EPS15
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat EPS15
    Sirion Biotech Customized lentivirus for stable overexpression of EPS15 
                         Customized lentivirus expression plasmids for stable overexpression of EPS15 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for EPS15
    Search LifeMap BioReagents cell lines for EPS15
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EPS15


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for EPS15 About   (see all 11)                                                                                              See pathways by source

    SuperPathContained pathways About
    1Endocytic Trafficking of EGFR
    Endocytic Trafficking of EGFR0.36
    EGFR downregulation0.30
    2Signaling by FGFR
    Signaling by EGFR0.84
    Signaling by EGFR in Cancer0.83
    3Clathrin-dependent protein traffic
    Transport Clathrin-coated vesicle cycle0.66
    wtCFTR and delta508 traffic / Clathrin coated vesicles formation (norm and CF)0.35
    4Signaling by GPCR
    Signal Transduction0.55
    5Development HGF signaling pathway
    Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)0.31

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of QIAGEN Pathway Central Maps for EPS15
        Endocytic Trafficking of EGFR

    1 Cell Signaling Technology (CST) Pathway for EPS15
        Tyrosine Kinases / Adaptors

    2 GeneGo (Thomson Reuters) Pathways for EPS15
        wtCFTR and delta508 traffic / Clathrin coated vesicles formation (norm and CF)
    Transport Clathrin-coated vesicle cycle

    4 BioSystems Pathways for EPS15
        EGFR1 Signaling Pathway
    Internalization of ErbB1
    Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
    Notch signaling pathway


    5        Reactome Pathways for EPS15
        Signal Transduction
    Signaling by EGFR in Cancer
    Signaling by EGFR
    Disease
    EGFR downregulation


    1         Kegg Pathway  (Kegg details for EPS15):
        Endocytosis


    EPS15 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for EPS15

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/162 Interacting proteins for EPS15 (P425661, 2, 3 ENSP000003607984) via UniProtKB, MINT, STRING, and/or I2D (see all 162)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    STON2Q8WXE92, 3, ENSP000002675404MINT-6180038 MINT-6180111 MINT-6180129 MINT-6180018 MINT-6179779 MINT-6179905 MINT-6180093 MINT-6179941 MINT-6180075 MINT-6179996 MINT-6179890 MINT-6179923 MINT-6179875 MINT-6179857 MINT-6179977 MINT-6180056 I2D: score=4 STRING: ENSP00000267540
    NUMBP497572, 3, ENSP000003471694MINT-17946 MINT-17947 I2D: score=5 STRING: ENSP00000347169
    AGFG1P525942, 3MINT-50023 MINT-50024 I2D: score=5 
    LAPTM5Q135712, 3, ENSP000002945074MINT-62182 I2D: score=3 STRING: ENSP00000294507
    NCK1P163331, 3, ENSP000002889864EBI-396684,EBI-389883 I2D: score=2 STRING: ENSP00000288986
    About this table

    Gene Ontology (GO): 5/9 biological process terms (GO ID links to tree view) (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006308DNA catabolic process ----
    GO:0006897endocytosis IEA--
    GO:0007173epidermal growth factor receptor signaling pathway TAS--
    GO:0008283cell proliferation TAS8183552
    GO:0015031protein transport IEA--

    EPS15 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    EPS15 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for EPS15

    5 Novoseek inferred chemical compound relationships for EPS15 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tyrosine 47.2 30 10953014 (5), 19109251 (3), 7689153 (2), 9162018 (2) (see all 16)
    phosphotyrosine 37.8 6 15465819 (1), 10953014 (1), 15155807 (1)
    cholesterol 0 2 16919152 (1), 17919781 (1)
    lipid 0 1 16887992 (1)
    phosphatidylinositol 0 2 17412695 (2)

    Search CenterWatch for drugs/clinical trials and news about EPS15

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for EPS15 gene (2 alternative transcripts): 
    NM_001159969.1  NM_001981.2  

    Unigene Cluster for EPS15:

    Epidermal growth factor receptor pathway substrate 15
    Hs.83722  [show with all ESTs]
    Unigene Representative Sequence: NM_001981
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000371730 ENST00000371733(uc001csp.3 uc001csq.1 uc009vyz.1)
    ENST00000486505 ENST00000478657 ENST00000493793 ENST00000371727 ENST00000464478
    ENST00000471391 ENST00000465467 ENST00000396122

    miRNA
    Products:
         
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat EPS15
    8/78 QIAGEN miScript miRNA Assays for microRNAs that regulate EPS15 (see all 78):
    hsa-miR-607 hsa-miR-374a hsa-miR-138-2* hsa-miR-371-5p hsa-miR-556-3p hsa-miR-200b hsa-miR-130b hsa-miR-130a*
    SwitchGear 3'UTR luciferase reporter plasmidEPS15 3' UTR sequence
    Inhib. RNA
    Products:
         
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for EPS15
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat EPS15
    Clone
    Products:
         
    OriGene clones in human, mouse for EPS15 (see all 12)
    OriGene ORF clones in mouse, rat for EPS15
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): EPS15 (NM_001981)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for EPS15
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat EPS15
    Sirion Biotech Customized lentivirus for stable overexpression of EPS15 
                         Customized lentivirus expression plasmids for stable overexpression of EPS15 
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for EPS15
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat EPS15
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat EPS15
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat EPS15

    Additional mRNA sequence: 

    AF052132.1 AK090936.1 AK129853.1 AK294297.1 AK308128.1 AK313396.1 BC054006.1 BX647676.1 
    DQ367924.1 U07707.1 Z29064.1 Z29070.1 

    11 DOTS entries:

    DT.100777918  DT.447690  DT.95290133  DT.95290139  DT.100008201  DT.99946098  DT.95290125  DT.91771128 
    DT.95290138  DT.100737445  DT.97779149 

    24/287 AceView cDNA sequences (see all 287):

    D87737 AI690679 AA195277 AF052132 AW368006 NM_001981 AA789139 AI208237 
    CA414407 AW138223 CR599353 AA960841 AL043493 BF795094 AI864625 AA331589 
    AI214295 BM727791 AW949896 AL043535 BX340738 AI003407 AI472645 F10989 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for EPS15    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18a · 18b ^ 19 ^ 20 ^ 21 ^ 22 ^ 23 ^
    SP1:              -     -     -                                               -                             -                                                   
    SP2:              -     -     -                                               -                             -                       -     -                     
    SP3:                                                                                                                          -     -                           
    SP4:              -     -     -                                                                                                                                 
    SP5:                                                                                                                                                            

    ExUns: 24 ^ 25 ^ 26 ^ 27 ^ 28 ^ 29
    SP1:                                    
    SP2:                                    
    SP3:                                    
    SP4:                                    
    SP5:                                    


    ECgene alternative splicing isoforms for EPS15

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    EPS15 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CTGTGTCCAA
    EPS15 Expression
    About this image


    See EPS15 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for EPS15

    SOURCE GeneReport for Unigene cluster: Hs.83722

    UniProtKB/Swiss-Prot: EPS15_HUMAN, P42566
    Tissue specificity: Ubiquitously expressed

        SABiosciences Custom PCR Arrays for EPS15
    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for EPS15
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat EPS15
    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat EPS15
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat EPS15
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EPS15

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals and fungi.

    Orthologs for EPS15 gene from 7/14 species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Eps151 , 5 epidermal growth factor receptor pathway substrate more1, 5 89.45(n)1
    89.6(a)1
      4 (51.29 cM)5
    138581  NM_007943.31  NP_031969.11 
     1092802685 
    chicken
    (Gallus gallus)
    Aves EPS151 epidermal growth factor receptor pathway substrate more 77.64(n)
    77.9(a)
      424633  NM_001031283.1  NP_001026454.1 
    lizard
    (Anolis carolinensis)
    Reptilia EPS156
    epidermal growth factor receptor pathway substrate...
    72(a)
    1 ↔ 1
    4(113993467-114075163)
    zebrafish
    (Danio rerio)
    Actinopterygii LOC7955051 similar to EPS15 protein 59.32(n)
    59.7(a)
      795505  XM_002663099.2  XP_002663145.2 
    fruit fly
    (Drosophila melanogaster)
    Insecta Eps-156
    Epidermal growth factor receptor pathway substrate...
    21(a)
    1 → many
    2R(20655837-20662788)
    worm
    (Caenorhabditis elegans)
    Secernentea ehs-16
    Protein EHS-1, isoform a
    27(a)
    1 → many
    II(5820710-5824085)
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes EDE1(YBL047C)4 Key endocytic protein involved in a network of interactions more   --   2(132040-127895) 852233  NP_009506.1 


    ENSEMBL Gene Tree for EPS15 (if available)
    TreeFam Gene Tree for EPS15 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for EPS15 gene
    REPS12  EPS15L12  EHD32  REPS22  EHD22  EHD12  EHD42  ITSN12  
    ITSN22  SRL2  
    14 SIMAP similar genes for EPS15 using alignment to 4 protein entries:     EPS15_HUMAN (see all proteins):
    FAM175A    HMGA2    LINC00596    PTPLA    AURA2    NFS1
    EPS15L1    NF2    FP1523    DNAPTP3    GLT8D2    EIF3L
    BCMA    DKFZp586A0524

    EPS15 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/3334 SNPs in EPS15 are shown (see all 3334)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs679200391,2
    C--50014457(-) ATTTT-/TGTGTGT 1 -- int11Minor allele frequency- TG:0.00NA 2
    rs1856889101,2
    --51819574(+) TGAGAC/TGGAGT 2 -- ds50010--------
    rs112058371,2
    C,A,H--51819592(+) tgtcaC/Tccagg 2 -- ds50010--------
    rs1885508301,2
    --51819621(+) ATCTCA/GGCTCA 2 -- ds50010--------
    rs1921095271,2
    --51819676(+) CCTCCC/TGAGTA 2 -- ds50010--------
    rs120704161,2
    F--51819717(+) TGCTAA/TTTTTT 2 -- ds50012Minor allele frequency- T:0.05NA WA 120
    rs1838370871,2
    --51819751(+) TTTCAC/TCATGT 2 -- ds50010--------
    rs1455458531,2
    --51819808(+) TTGGCA/CTCCCA 2 -- ds50010--------
    rs24056981,2
    --51819833(+) caggcA/Gcgagc 2 -- ds50010--------
    rs794314741,2
    C,F--51819835(+) GGCGCG/AAGCCA 2 -- ds50012Minor allele frequency- A:0.04CSA NA 122

    HapMap Linkage Disequilibrium report for EPS15 (51819935 - 51985000 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 4 variations for EPS15:    About this table     
    Variant IDTypeSubtypePubMed ID
    esv2659806CNV Deletion23128226
    esv272092CNV Insertion20981092
    esv273142CNV Insertion20981092
    esv25434CNV Loss19812545

    SABiosciences Cancer Mutation PCR Assays
    SeqTarget long-range PCR primers for resequencing EPS15
    DNA2.0 Custom Variant and Variant Library Synthesis for EPS15

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 600051    OMIM disorders: --

    UniProtKB/Swiss-Prot: EPS15_HUMAN, P42566
  • Note=A chromosomal aberration involving EPS15 is found in acute leukemias. Translocation t(1;11)(p32;q23)
    with KMT2A/MLL1. The result is a rogue activator protein

  • 11 diseases for EPS15:    About MalaCards
    troyer syndrome    menkes disease    down syndrome    vaccinia
    leukemia    acute leukemia    allergic rhinitis    rhinitis
    hepatocellular carcinoma    endotheliitis    breast cancer


    EPS15 for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:  
    The International Symposium on Pneumococci and Pneumococcal Diseases (ISPPD) 9 - 13 March 2014

    2 Novoseek inferred disease relationships for EPS15 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    down syndrome 11.5 1 14525795 (1)
    tumors 0 2 7791787 (1), 10481267 (1)

    Genetic Association Database (GAD): EPS15
    Human Genome Epidemiology (HuGE) Navigator: EPS15 (2 documents)

    Export disorders for EPS15 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for EPS15 gene, integrated from 9 sources (see all 216):
    (articles sorted by number of sources associating them with EPS15)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The human eps15 gene, encoding a tyrosine kinase substrate, is conserved in evolution and maps to 1p31-p32. (PubMed id 8183552)1, 2, 3, 9 Wong W.T.... di Fiore P.P. (1994)
    2. An endosomally localized isoform of Eps15 interacts with Hrs to mediate degradation of epidermal growth factor receptor. (PubMed id 18362181)1, 2, 9 Roxrud I.... Stenmark H. (2008)
    3. STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes. (PubMed id 12551915)1, 2, 9 Bache K.G.... Stenmark H. (2003)
    4. Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis. (PubMed id 9723620)1, 2, 9 Chen H.... De Camilli P. (1998)
    5. Structure and Asn-Pro-Phe binding pocket of the Eps15 homology domain. (PubMed id 9721102)1, 2, 9 de Beer T.... Overduin M. (1998)
    6. Solution structure of Eps15's third EH domain reveals coincident Phe- Trp and Asn-Pro-Phe binding sites. (PubMed id 10757979)1, 2, 9 Enmon J.L.... Overduin M. (2000)
    7. Molecular mechanism of NPF recognition by EH domains. (PubMed id 11062555)1, 2, 9 de Beer T.... Overduin M. (2000)
    8. The clathrin adaptor Dab2 recruits EH domain scaffold proteins to regulate integrin beta1 endocytosis. (PubMed id 22648170)1, 2 Teckchandani A.... Cooper J.A. (2012)
    9. Characterization of the EFC/F-BAR domain protein, FCH O2. (PubMed id 21762413)1, 2 Uezu A....Nakanishi H. (2011)
    10. A genome-wide meta-analysis of genetic variants associ ated with allergic rhinitis and grass sensitization and their interaction with b irth order. (PubMed id 22036096)1, 4 Ramasamy A....Jarvis D.L. (2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 2060 HGNC: 3419 AceView: EPS15 Ensembl:ENSG00000085832 euGenes: HUgn2060
    ECgene: EPS15 Kegg: 2060 H-InvDB: EPS15

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for EPS15 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for EPS15 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for EPS15 gene:
    Search GeneIP for patents involving EPS15

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

     
     EMD Millipore Custom Antibody & Bulk Services
     EMD Millipore Preclinical / Clinical Development Services
     EMD Millipore Immunoassay Services
     EMD Millipore Target Screening & Profiling Services

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Kinase Activity Assays/Reagents  
     Browse ELISpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Multiplex/Array Assay Kits/Reagents  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Protease Activity Assays and Reagents   Browse Recombinant/Natural Proteins  
     Browse Stem Cell Products   Browse Tocris Biochemicals & Compounds  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for EPS15   OriGene RNAi products in human, mouse, rat for EPS15  
     Browse OriGene qPCR primer pairs and template standards   OriGene Protein Over-expression Lysate for EPS15  
     OriGene MassSpec something-or-other for EPS15   OriGene clones in human, mouse for EPS15  
     OriGene qSTAR qPCR primer pairs in human, mouse for EPS15   OriGene Purified Protein for EPS15  
     OriGene ORF clones in mouse, rat for EPS15   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for EPS15   OriGene Custom Protein Services for EPS15  

     
     
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat EPS15
     QIAGEN SeqTarget long-range PCR primers for resequencing EPS15
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat EPS15
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat EPS15
     QIAGEN QuantiFast Probe-based Assays in human, mouse, rat EPS15
     QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat EPS15
     GenScript Custom Purified and Recombinant Proteins Services for EPS15 GenScript cDNA clones with any tag delivered in your preferred vector for EPS15
     GenScript Custom Assay Services for EPS15 GenScript Custom Superior Antibodies Services for EPS15
     GenScript Custom overexpressing Cell Line Services for EPS15 CloneReady with Over 120,000 Genes
     Gene Synthesis: Any Gene in Any Vector Vector-based siRNA and miRNA, Ready for Transfection
     Gene Mutant Library, Variants up to 10^11 Plasmid Preparation
     Custom Peptide Services
     Antibodies & Assays for EPS15 

     Regulatory tfbs in EPS15 promoter
     Search Chromatin IP Primers for EPS15
     RT2 qPCR Primer Assay in human, mouse, rat EPS15
     GNC Network for EPS15
     SABiosciences PCR Arrays including human, mouse, rat EPS15
     Search Tocris compounds for EPS15
     Browse Sino Biological Proteins and Antibodies
     Browse Sino Biological Cell Lysates
     cDNA Clones for EPS15
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
     EPS15 antibodies
     EPS15 lysates
     Antibodies for EPS15
     See all of Abcam's Antibodies, Kits and Proteins for EPS15
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins




     Proteins for EPS15
     Antibodies for EPS15
     ELISAs for EPS15
     CLIAs for EPS15
     Search LifeMap BioReagents cell lines for EPS15
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EPS15
     SwitchGear 3'UTR luciferase reporter plasmids for EPS15
     SwitchGear Promoter luciferase reporter plasmids for EPS15
     ThermoFisher Antibodies for EPS15
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat EPS15
     inGenious Targeting Laboratory - Custom generated mouse model solutions for EPS15
     inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for EPS15
    Customized:
     lentivirus for stable overexpression of EPS15
     lentivirus expression plasmids for stable overexpression of EPS15
     adenovirus for overexpression of EPS15
     LSBio Antibodies in human, mouse, rat for EPS15
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
           
    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 4 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

    View Random Gene

    Category
    (GIFtS: 73)
    transforming growth factor, beta 1
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      EPS15 gene at Home site.
    hostname: 356977-web1.xennexinc.com index build: 106 solr: 1.4