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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

EPHB1 Gene

protein-coding GIFtS: 65
GCID: GC03P134316

EPH receptor B1

(Previous name: EphB1 )
(Previous symbol: EPHT2)

Explore 14 diseases affiliated with
EPHB1 via

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(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
EPH Receptor B1¹ ²		EPH-Like Kinase 6² ³
EPHT2¹ ² ³ ⁵		EC 2.7.10.1³ ⁸
NET² ³ ⁵		EphB1¹
Hek6¹ ²		Ephrin Type-B Receptor 1²
EPH Tyrosine Kinase 2² ³		Soluble EPHB1 Variant 1²
Neuronally-Expressed EPH-Related Tyrosine Kinase² ³		HEK6³
Tyrosine-Protein Kinase Receptor EPH-2² ³		HEK6³
EK6² ³		EC 2.7.10⁸
ELK² ³

External Ids:	HGNC: 3392¹	Entrez Gene: 2047²	Ensembl: ENSG00000154928⁷	OMIM: 600600⁵	UniProtKB: P54762³

Export aliases for EPHB1 gene to outside databases

Previous GC identifers: GC03M131897 GC03P135607 GC03P135795 GC03P135835 GC03P135996 GC03P131890

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for EPHB1:

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous

system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class,

which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are

transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their

extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make

up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor

for ephrin-B family members. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: EPHB1_HUMAN, P54762

Function: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent

cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream

of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is

referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3.

During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal

ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the

adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural

progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in

development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally,

may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands

activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively

Gene Wiki entry for EPHB1 (EPH receptor B1)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000003.11 NC_018914.1 NT_005612.16

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the EPHB1 gene promoter:
SRF Spz1 SRF (504 AA) CUTL1 STAT5A Nkx2-5 Arnt AREB6 HOXA5 NF-kappaB1
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: EPHB1 promoter sequence

Search SABiosciences Chromatin IP Primers for EPHB1

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat EPHB1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q21-q23 Ensembl cytogenetic band: 3q22.2 HGNC cytogenetic band: 3q21-q23

EPHB1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
EPHB1 gene location

GeneLoc information about chromosome 3 GeneLoc Exon Structure

GeneLoc location for GC03P134316: view genomic region (about GC identifiers)

Start:	134,316,643 bp from pter	End:	134,979,309 bp from pter
Size:	662,667 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: EPHB1_HUMAN, P54762 (See protein sequence)

Recommended Name: Ephrin type-B receptor 1 precursor

Size: 984 amino acids; 109885 Da

Subunit: Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor

dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts with EPHB6;

transphosphorylates EPHB6 to form an active signaling complex. Interacts with PICK1 (By similarity). Interacts

(through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion (By similarity). The

ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form

interacts (residues within the catalytic domain) with GRB2 (via SH2 domain). Interacts with GRB2, SHC1 and SRC;

activates the MAPK/ERK cascade to regulate cell migration. Interacts with CBL; regulates receptor degradation through

ubiquitination. Interacts with ACP1

Subcellular location: Cell membrane; Single-pass type I membrane protein. Early endosome membrane. Cell projection,

dendrite (By similarity)

Sequence caution: Sequence=AAB94627.1; Type=Miscellaneous discrepancy; Note=wrong intron-exon boundaries;

Sequence=AAB94628.1; Type=Miscellaneous discrepancy; Note=wrong intron-exon boundaries; Sequence=AAD02031.1;

Type=Miscellaneous discrepancy; Note=Chimeric cDNA;

2 PDB 3D structures from and Proteopedia for EPHB1:

2DJS (3D)

2EAO (3D)

Secondary accessions: A8K593 O43569 O95142 O95143 Q0VG87

Explore the universe of human proteins at neXtProt for EPHB1: NX_P54762

Post-translational modifications:

Phosphorylated. Autophosphorylation is stimulated by the ligand EFNB1. Required for interaction with SH2

domain-containing interactors, for activation of the MAPK/ERK and JUN signaling cascades and for ubiquitination by CBL¹

Ubiquitinated; (EFNB1)ligand-induced poly- and/or multi-ubiquitination by CBL is regulated by SRC and leads to

lysosomal degradation¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P54762

4/65 DME Specific Peptides for EPHB1 (P54762) (see all 65)

RDLAARN

CKETFNL

SLQLVTQ

EDLLRIG

EPHB1 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_004432.1

ENSEMBL proteins:

ENSP00000417435 ENSP00000420338 ENSP00000381097 ENSP00000419688 ENSP00000417216

ENSP00000418352 ENSP00000419574

Human Recombinant Protein Products:

	EMD Millipore Purified and/or Recombinant EPHB1 Protein
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells Browse OriGene Protein Over-expression Lysates OriGene Custom Protein Services for EPHB1
	GenScript Custom Purified and Recombinant Proteins Services for EPHB1
	Novus Biologicals EPHB1 Proteins Novus Biologicals EPHB1 Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for EPHB1

Gene Ontology (GO): 5/8 cellular component terms (GO ID links to tree view) (see all 8): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005737	cytoplasm	--	--
GO:0005887	integral to plasma membrane	IDA	18034775
GO:0016020	membrane	--	--
GO:0016021	integral to membrane	--	--
GO:0030424	axon	IEA	--

EPHB1 for ontologies About GeneDecksing

EPHB1 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of EPHB1
	R&D Systems Antibodies for EPHB1
	Cell Signaling Technology (CST) Antibodies for EPHB1
	OriGene Antibodies: EPHB1 OriGene Custom Antibody Services for EPHB1
	GenScript Custom Superior Antibodies Services for EPHB1
	Novus Biologicals EPHB1 Antibodies
	Abcam antibodies for EPHB1
	Uscn Antibodies for EPHB1
	ThermoFisher Antibody for EPHB1

Assay Products for EPHB1:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	R&D Systems Multiplex/Array Assay Kits & Reagents for EPHB1
	GenScript Custom Assay Services for EPHB1
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for EPHB1

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

EPHB1 for domains About GeneDecksing

5/15 InterPro domains/families (see all 15):

IPR017441 Protein_kinase_ATP_BS

IPR003961 Fibronectin_type3

IPR021129 SAM_type1

IPR011009 Kinase-like_dom

IPR013783 Ig-like_fold

Graphical View of Domain Structure for InterPro Entry P54762

ProtoNet protein and cluster: P54762

5 Blocks protein families:

IPB001090 Ephrin receptor

IPB001426 Receptor tyrosine kinase

IPB001660 Sterile alpha motif SAM

IPB003962 Fibronectin type III repeat signature

IPB008266 Tyrosine protein kinase

UniProtKB/Swiss-Prot: EPHB1_HUMAN, P54762

Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily

Similarity: Contains 1 Eph LBD (Eph ligand-binding) domain

Similarity: Contains 2 fibronectin type-III domains

Similarity: Contains 1 protein kinase domain

Similarity: Contains 1 SAM (sterile alpha motif) domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary: