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ENSG00000229094 Gene

protein-coding   GIFtS: 16
GCID: GC06Mk31120          (predicted)

ENSG00000229094


  Search for ENSG00000229094
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
OCT33     Oct-43
OCT43     Octamer-Binding Protein 33
OTF-33     Octamer-Binding Protein 43
OTF33     Octamer-Binding Transcription Factor 33
Oct-33     

External Ids:    Ensembl: ENSG000002290947   UniProtKB: Q018603   

Export aliases for ENSG00000229094 gene to outside databases


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for ENSG00000229094 Gene:
ENSG00000229094 is a protein-coding gene.

UniProtKB/Swiss-Prot: PO5F1_HUMAN, Q01860
Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with
SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1,
FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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Regulatory elements:
   Search for regulatory transcription factor binding sites for ENSG00000229094
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search Chromatin IP Primers for ENSG00000229094

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ENSG00000229094


Genomic Location:
Chromosome:6   

Ensembl cytogenetic band:  HSCHR6_MHC_DBBp21.33   

GeneLoc information about chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06Mk31120:       (about GC identifiers)

Start:
31,120,916 bp from start of contig ALT_REF_LOCI_3      End:
31,132,215 bp from start of contig ALT_REF_LOCI_3
Size:
11,300 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: PO5F1_HUMAN, Q01860 (See protein sequence)
Recommended Name: POU domain, class 5, transcription factor 1  
Size: 360 amino acids; 38571 Da
Subunit: Interacts with UBE2I and ZSCAN10 (By similarity). Interacts with PKM. Interacts with WWP2
Developmental stage: Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually
after embryoid body (EB) formation
Biotechnology: POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors
are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent
stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes
Miscellaneous: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them,
localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved
in the regulation of POU5F1 gene activity in carcinogenesis
Secondary accessions: A6NCS1 A6NLL8 D2IYK4 P31359 Q15167 Q15168 Q16422 Q5STF3 Q5STF4
Alternative splicing: 2 isoforms:  Q01860-1   Q01860-2   

Explore the universe of human proteins at neXtProt for ENSG00000229094: NX_Q01860

Explore proteomics data for ENSG00000229094 at MOPED

Post-translational modifications: 

  • Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for
    enhanced YES1 expression (By similarity)1
  • Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation (By
    similarity)1
  • ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation
    at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See ENSG00000229094 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB


    ENSEMBL proteins: 
     ENSP00000387646   ENSP00000388842   ENSP00000448231   ENSP00000447969  
    Reactome Protein details: Q01860

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    Selected InterPro protein domains (see all 7):
     IPR013847 POU
     IPR000327 POU_specific
     IPR017970 Homeobox_CS
     IPR001356 Homeobox_dom
     IPR010982 Lambda_DNA-bd_dom

    Graphical View of Domain Structure for InterPro Entry Q01860

    ProtoNet protein and cluster: Q01860

    2 Blocks protein domains:
    IPB000327 POU domain
    IPB013847 POU


    UniProtKB/Swiss-Prot: PO5F1_HUMAN, Q01860
    Domain: The POU-specific domain mediates interaction with PKM
    Similarity: Belongs to the POU transcription factor family. Class-5 subfamily
    Similarity: Contains 1 homeobox DNA-binding domain
    Similarity: Contains 1 POU-specific domain


    Find genes that share domains with ENSG00000229094           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: PO5F1_HUMAN, Q01860
    Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with
    SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1,
    FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency
    Induction: Transcriptional activity is positively regulated by PKM

         Gene Ontology (GO): Selected molecular function terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000981sequence-specific DNA binding RNA polymerase II transcription factor activity ----
    GO:0003677DNA binding ----
    GO:0003700sequence-specific DNA binding transcription factor activity ----
    GO:0005515protein binding ----
    GO:0008134transcription factor binding ----
         
    Find genes that share ontologies with ENSG00000229094           About GenesLikeMe


    Animal Models:
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    PO5F1_HUMAN, Q01860: Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern

    Gene Ontology (GO): 5 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus ----
    GO:0005654nucleoplasm ----
    GO:0005667transcription factor complex ----
    GO:0005737cytoplasm ----
    GO:0005829cytosol ----

    Find genes that share ontologies with ENSG00000229094           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Find genes that share SuperPaths with ENSG00000229094           About GenesLikeMe

    Pathways by sourceSee SuperPaths
    Show all pathways


    3 Reactome Pathways for ENSG00000229094
        POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation
    Transcriptional regulation of pluripotent stem cells
    POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation


        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ENSG00000229094
    Interactions:

        Search GeneGlobe Interaction Network for ENSG00000229094

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for ENSG00000229094 (Q018601, 2, 3 ENSP000003888424) via UniProtKB, MINT, STRING, and/or I2D (see all 226)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=2 
    HIST1H4BP628053I2D: score=2 
    HIST1H4CP628053I2D: score=2 
    HIST1H4DP628053I2D: score=2 
    HIST1H4EP628053I2D: score=2 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 19):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001714endodermal cell fate specification ----
    GO:0001824blastocyst development ----
    GO:0003130BMP signaling pathway involved in heart induction ----
    GO:0006355regulation of transcription, DNA-templated ----
    GO:0006366transcription from RNA polymerase II promoter ----

    Find genes that share ontologies with ENSG00000229094           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ENSG00000229094 (PO5F1)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000463773 ENST00000412166(uc011gai.2 uc011gaj.2 uc021znh.1)
    ENST00000434616(uc011gak.2) ENST00000489610 ENST00000553069 ENST00000550521

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    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    See probesets specificity/sensitivity at GeneAnnot
    CGAP TAG: --

    ENSG00000229094 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ENSG00000229094 Protein Expression

    UniProtKB/Swiss-Prot: PO5F1_HUMAN, Q01860
    Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the
    olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues

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    In Situ
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    Search Advanced Cell Diagnostics for RNAscope RNA in situ hybridization assays for ENSG00000229094

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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      --

    ENSEMBL Gene Tree for ENSG00000229094 (if available)
    TreeFam Gene Tree for ENSG00000229094 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Site Specific Mutation Identification with PCR Assays
    Search QIAGEN SeqTarget long-range PCR primers for resequencing ENSG00000229094
    DNA2.0 Custom Variant and Variant Library Synthesis for ENSG00000229094

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ENSG00000229094 gene, integrated from 10 sources (see all 13):
    (articles sorted by number of sources associating them with ENSG00000229094)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Phosphorylation regulates human OCT4. (PubMed id 22474382)2 Brumbaugh J.... Thomson J.A. (Proc. Natl. Acad. Sci. U.S.A. 2012)
    2. Serine 111 phosphorylation regulates OCT4A protein subcellular distribution and degradation. (PubMed id 23024368)2 Spelat R.... Curcio F. (J. Biol. Chem. 2012)
    3. Comparison of MHC class I risk haplotypes in Thai and Caucasian psoriatics shows locus heterogeneity at PSORS1. (PubMed id 20604894)2 Stuart P.E.... Elder J.T. (Tissue Antigens 2010)
    4. WWP2 promotes degradation of transcription factor OCT4 in human embryonic stem cells. (PubMed id 19274063)2 Xu H.... Jin Y. (Cell Res. 2009)
    5. Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription. (PubMed id 18191611)2 Lee J.... Kim J. (Int. J. Biochem. Cell Biol. 2008)
    6. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. (PubMed id 18035408)2 Takahashi K....Yamanaka S. (Cell 2007)
    7. Rapid evolution of major histocompatibility complex class I genes in primates generates new disease alleles in humans via hitchhiking diversity. (PubMed id 16702430)2 Shiina T.... Bahram S. (Genetics 2006)
    8. Oct4 pseudogenes are transcribed in cancers. (PubMed id 16229821)2 Suo G.... Dai J. (Biochem. Biophys. Res. Commun. 2005)
    9. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    10. The DNA sequence and analysis of human chromosome 6. (PubMed id 14574404)2 Mungall A.J.... Beck S. (Nature 2003)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Ensembl:ENSG00000229094

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    Wikipedia http://en.wikipedia.org/wiki/Oct-4

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for ENSG00000229094 gene:
    Search GeneIP for patents involving ENSG00000229094

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

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