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Aliases for ENO1 Gene

Aliases for ENO1 Gene

  • Enolase 1, (Alpha) 2 3
  • MPB1 3 4 6
  • 2-Phospho-D-Glycerate Hydro-Lyase 3 4
  • Plasminogen-Binding Protein 3 4
  • Phosphopyruvate Hydratase 3 4
  • Non-Neural Enolase 3 4
  • EC 4 64
  • ENO1L1 3 4
  • PPH 3 6
  • NNE 3 4
  • MYC Promoter-Binding Protein 1 3
  • C-Myc Promoter-Binding Protein 4
  • Alpha Enolase Like 1 3
  • Tau-Crystallin 3
  • Alpha-Enolase 3
  • Enolase-Alpha 3
  • Enolase 1 4
  • EC 4.2.1 64
  • MBP-1 4
  • MBPB1 4
  • MPB-1 4

External Ids for ENO1 Gene

Previous Symbols for ENO1 Gene

  • ENO1L1
  • MPB1

Summaries for ENO1 Gene

Entrez Gene Summary for ENO1 Gene

  • This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

GeneCards Summary for ENO1 Gene

ENO1 (Enolase 1, (Alpha)) is a Protein Coding gene. Diseases associated with ENO1 include non-herpetic acute limbic encephalitis and limbic encephalitis. Among its related pathways are Metabolism and Carbon metabolism. GO annotations related to this gene include sequence-specific DNA binding transcription factor activity and transcription corepressor activity. An important paralog of this gene is ENO2.

UniProtKB/Swiss-Prot for ENO1 Gene

  • Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production

  • MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor

Gene Wiki entry for ENO1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ENO1 Gene

Genomics for ENO1 Gene

Genomic Location for ENO1 Gene

8,861,000 bp from pter
8,879,249 bp from pter
18,250 bases
Minus strand

Genomic View for ENO1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for ENO1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ENO1 Gene

Regulatory Elements for ENO1 Gene

Proteins for ENO1 Gene

  • Protein details for ENO1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Protein Accession:
    Secondary Accessions:
    • B2RD59
    • P22712
    • Q16704
    • Q4TUS4
    • Q53FT9
    • Q53HR3
    • Q658M5
    • Q6GMP2
    • Q71V37
    • Q7Z3V6
    • Q8WU71
    • Q96GV1
    • Q9BT62
    • Q9UCH6
    • Q9UM55

    Protein attributes for ENO1 Gene

    434 amino acids
    Molecular mass:
    47169 Da
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds two Mg(2+) per subunit. Required for catalysis and for stabilizing the dimer.;
    Quaternary structure:
    • Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding (By similarity).
    • Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism
    • Sequence=AAA35698.1; Type=Frameshift; Positions=Several; Evidence={ECO:0000305}; Sequence=AAA35698.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for ENO1 Gene

    Alternative splice isoforms for ENO1 Gene


neXtProt entry for ENO1 Gene

Proteomics data for ENO1 Gene at MOPED

Post-translational modifications for ENO1 Gene

  • ISGylated.
  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys5, Lys28, Lys54, Lys60, Lys64, Lys71, Lys81, Lys89, Lys120, Lys126, Lys193, Lys197, Lys202, Lys221, Lys228, Lys233, Lys256, Lys262, Lys281, Lys285, Lys326, Lys335, Lys343, Lys358, Lys394, Lys406, and Lys420

Other Protein References for ENO1 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Domains for ENO1 Gene

Protein Domains for ENO1 Gene


  • Belongs to the enolase family.:
    • P06733
genes like me logo Genes that share domains with ENO1: view

No data available for Gene Families for ENO1 Gene

Function for ENO1 Gene

Molecular function for ENO1 Gene

GENATLAS Biochemistry: enolase 1,alpha subunit,ubiquitous and embryonic precocious type (2-phospho-D-glycerate hydratase),glycolysis,energy pathway
UniProtKB/Swiss-Prot BiophysicochemicalProperties: pH dependence: Enolase activity is lost above pH 9.0. Immunoglobulin production stimulating activity is retained at pH 13.0. {ECO:0000269 PubMed:1369209};
UniProtKB/Swiss-Prot CatalyticActivity: 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.
UniProtKB/Swiss-Prot Function: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production
UniProtKB/Swiss-Prot Function: MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor
UniProtKB/Swiss-Prot Induction: Induced in diffuse large cell lymphoma (DLCL) after treatment with the natural biological agent, Bryo1. Up-regulated in response to enterovirus 71 (EV71) infection (at protein level).

Enzyme Numbers (IUBMB) for ENO1 Gene

Gene Ontology (GO) - Molecular Function for ENO1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding IEA --
GO:0003677 DNA binding IEA --
GO:0003700 sequence-specific DNA binding transcription factor activity TAS 2005901
GO:0003714 transcription corepressor activity TAS 2005901
GO:0004634 phosphopyruvate hydratase activity IEA --
genes like me logo Genes that share ontologies with ENO1: view

No data available for Phenotypes , Animal Models , Transcription Factor Targeting and HOMER Transcription for ENO1 Gene

Localization for ENO1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ENO1 Gene

Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Isoform MBP-1: Nucleus.

Subcellular locations from

Jensen Localization Image for ENO1 Gene COMPARTMENTS Subcellular localization image for ENO1 gene
Compartment Confidence
extracellular 5
nucleus 5
plasma membrane 5
cytosol 4
cytoskeleton 2
mitochondrion 1

Gene Ontology (GO) - Cellular Components for ENO1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000015 phosphopyruvate hydratase complex IEA --
GO:0005615 extracellular space IDA 16502470
GO:0005634 nucleus IDA 21630459
GO:0005737 cytoplasm IDA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with ENO1: view

Pathways for ENO1 Gene

genes like me logo Genes that share pathways with ENO1: view

Pathways by source for ENO1 Gene

UniProtKB/Swiss-Prot P06733-ENOA_HUMAN

  • Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5

Gene Ontology (GO) - Biological Process for ENO1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter TAS 2005901
GO:0005975 carbohydrate metabolic process TAS --
GO:0006006 glucose metabolic process TAS --
GO:0006094 gluconeogenesis TAS --
GO:0006096 glycolytic process --
genes like me logo Genes that share ontologies with ENO1: view

Compounds for ENO1 Gene

(5) HMDB Compounds for ENO1 Gene

Compound Synonyms Cas Number PubMed IDs
2-Phospho-D-glyceric acid
  • 2-Phospho-D-glycerate
Not Available
  • 3-Dehydroquinic acid
Not Available
  • Magnesium
Phosphoenolpyruvic acid
  • 2-hydroxy-Acrylic acid dihydrogen phosphate
  • Dihydrogen oxide

(32) Novoseek inferred chemical compound relationships for ENO1 Gene

Compound -log(P) Hits PubMed IDs
2-phosphoglycerate 76 2
glyceraldehyde 3-phosphate 75.1 35
lysine 50 3
4-hydroxynonenal 44.7 4
phosphoenolpyruvate 39.1 4
genes like me logo Genes that share compounds with ENO1: view

Transcripts for ENO1 Gene

Unigene Clusters for ENO1 Gene

Enolase 1, (alpha):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for ENO1 Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b · 3c ^ 4a · 4b ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14
SP1: - - -
SP2: - - - - - -
SP3: -
SP4: -

Relevant External Links for ENO1 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for ENO1 Gene

mRNA expression in normal human tissues for ENO1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for ENO1 Gene

SOURCE GeneReport for Unigene cluster for ENO1 Gene Hs.517145

mRNA Expression by UniProt/SwissProt for ENO1 Gene

Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
genes like me logo Genes that share expressions with ENO1: view

Orthologs for ENO1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for ENO1 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia ENO1 36
  • 99.39 (n)
  • 100 (a)
ENO1 37
  • 100 (a)
(Bos Taurus)
Mammalia -- 37
  • 96 (a)
-- 37
  • 63 (a)
ENO1 36
  • 90.32 (n)
  • 95.62 (a)
(Canis familiaris)
Mammalia ENO1 37
  • 65 (a)
(Mus musculus)
Mammalia Eno1 37
  • 95 (a)
Gm5506 36
  • 88.25 (n)
  • 94.7 (a)
Gm5506 16
Gm5506 37
  • 95 (a)
(Monodelphis domestica)
Mammalia -- 37
  • 82 (a)
-- 37
  • 90 (a)
(Ornithorhynchus anatinus)
Mammalia ENO1 37
  • 92 (a)
(Rattus norvegicus)
Mammalia Eno1 36
  • 88.33 (n)
  • 94.7 (a)
(Gallus gallus)
Aves ENO1 36
  • 83.45 (n)
  • 93.5 (a)
ENO1 37
  • 93 (a)
(Anolis carolinensis)
Reptilia ENO1 37
  • 73 (a)
African clawed frog
(Xenopus laevis)
Amphibia eno1-prov 36
tropical clawed frog
(Silurana tropicalis)
Amphibia eno1 36
  • 77.7 (n)
  • 88.43 (a)
MGC75746 36
(Danio rerio)
Actinopterygii eno1a 36
  • 81.71 (n)
  • 90.28 (a)
eno1a 37
  • 91 (a)
wufi32b03 36
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP007827 36
  • 70.53 (n)
  • 76.33 (a)
fruit fly
(Drosophila melanogaster)
Insecta Eno 36
  • 69.53 (n)
  • 73.09 (a)
Eno 37
  • 63 (a)
Eno 38
  • 72 (a)
(Caenorhabditis elegans)
Secernentea enol-1 36
  • 69.84 (n)
  • 75.64 (a)
enol-1 37
  • 70 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ENO1 37
  • 63 (a)
ENO2 37
  • 62 (a)
ENO2 39
ERR1 37
  • 59 (a)
ERR2 36
  • 58.14 (n)
  • 59.53 (a)
ERR2 37
  • 59 (a)
ERR3 37
  • 59 (a)
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.7421 36
thale cress
(Arabidopsis thaliana)
eudicotyledons ENO1 36
  • 62.88 (n)
  • 69.19 (a)
(Hordeum vulgare)
Liliopsida Hv.12462 36
(Zea mays)
Liliopsida Zm.410 36
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.4682 36
sea squirt
(Ciona savignyi)
Ascidiacea CSA.10219 37
  • 74 (a)
Species with no ortholog for ENO1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ENO1 Gene

Gene Tree for ENO1 (if available)
Gene Tree for ENO1 (if available)

Paralogs for ENO1 Gene

Paralogs for ENO1 Gene

Selected SIMAP similar genes for ENO1 Gene using alignment to 4 proteins: Pseudogenes for ENO1 Gene

genes like me logo Genes that share paralogs with ENO1: view

Variants for ENO1 Gene

Sequence variations from dbSNP and Humsavar for ENO1 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs1006950 -- 8,871,404(-) ATAGG(A/G)GCCCT utr-variant-5-prime, intron-variant
rs1006951 -- 8,871,304(-) AATTA(C/G)AGAAA utr-variant-5-prime, intron-variant
rs1059149 -- 8,861,097(-) GTCTA(A/C/G)AGTCA utr-variant-3-prime
rs1059176 -- 8,861,074(-) GTCAT(C/T)TCCGG utr-variant-3-prime
rs1065722 -- 8,861,372(-) AACCC(C/T)TTGGC reference, synonymous-codon

Structural Variations from Database of Genomic Variants (DGV) for ENO1 Gene

Variant ID Type Subtype PubMed ID
nsv871694 CNV Gain 21882294
nsv871026 CNV Loss 21882294

Relevant External Links for ENO1 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

Disorders for ENO1 Gene

(1) University of Copenhagen DISEASES for ENO1 Gene

(48) Novoseek inferred disease relationships for ENO1 Gene

Disease -log(P) Hits PubMed IDs
shock 51.2 29
autoimmune diseases 41.2 8
encephalopathy 37.1 9
autoimmunity 36.8 3
rheumatoid arthritis 35.9 7

Relevant External Links for ENO1

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
genes like me logo Genes that share disorders with ENO1: view

No data available for UniProtKB/Swiss-Prot for ENO1 Gene

Publications for ENO1 Gene

  1. Molecular cloning and expression analysis of five novel genes in chromosome 1p36. (PMID: 9653645) Onyango P. … Weith A. (Genomics 1998) 2 3 4 23
  2. Physical mapping of the CA6, ENO1, and SLC2A5 (GLUT5) genes and reassignment of SLC2A5 to 1p36.2. (PMID: 9691177) White P.S. … Brodeur G.M. (Cytogenet. Cell Genet. 1998) 2 3 23
  3. Anti-enolase-alpha autoantibodies in cancer-associated retinopathy: epitope mapping and cytotoxicity on retinal cells. (PMID: 9878089) Adamus G. … Machnicki M. (J. Autoimmun. 1998) 3 4 23
  4. Structure of the human gene for alpha-enolase. (PMID: 2373081) Giallongo A. … Feo S. (Eur. J. Biochem. 1990) 3 4 23
  5. Identification of an epitope of alpha-enolase (a candidate plasminogen receptor) by phage display. (PMID: 9308760) Arza B. … Munoz-Canoves P. (Thromb. Haemost. 1997) 3 4 23

Products for ENO1 Gene

  • Addgene plasmids for ENO1

Sources for ENO1 Gene

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