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Aliases for ENAH Gene

Aliases for ENAH Gene

  • Enabled Homolog (Drosophila) 2 3
  • MENA 3 4 6
  • NDPP1 3 6
  • ENA 3 6
  • Mammalian Enabled Variant 11a 3
  • Mammalian Enabled Variant Pan 3
  • Protein Enabled Homolog 3
  • Mammalian Enabled 2

External Ids for ENAH Gene

Previous GeneCards Identifiers for ENAH Gene

  • GC01M224165
  • GC01M221499
  • GC01M222090
  • GC01M222657
  • GC01M222659
  • GC01M221989
  • GC01M223741
  • GC01M225674
  • GC01M196193

Summaries for ENAH Gene

GeneCards Summary for ENAH Gene

ENAH (Enabled Homolog (Drosophila)) is a Protein Coding gene. Among its related pathways are Regulation of actin cytoskeleton and Regulation of actin cytoskeleton. GO annotations related to this gene include actin binding and WW domain binding. An important paralog of this gene is SPRED1.

UniProtKB/Swiss-Prot for ENAH Gene

  • Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity).

Gene Wiki entry for ENAH Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ENAH Gene

Genomics for ENAH Gene

Regulatory Elements for ENAH Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for ENAH Gene

Start:
225,486,832 bp from pter
End:
225,653,143 bp from pter
Size:
166,312 bases
Orientation:
Minus strand

Genomic View for ENAH Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for ENAH Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ENAH Gene

Proteins for ENAH Gene

  • Protein details for ENAH Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8N8S7-ENAH_HUMAN
    Recommended name:
    Protein enabled homolog
    Protein Accession:
    Q8N8S7
    Secondary Accessions:
    • D0PQI2
    • Q502W5
    • Q5T5M7
    • Q5VTQ9
    • Q5VTR0
    • Q9NVF3
    • Q9UFB8

    Protein attributes for ENAH Gene

    Size:
    591 amino acids
    Molecular mass:
    66510 Da
    Quaternary structure:
    • Homotetramer (By similarity). Interacts with APBB1IP, APBB1, PFN1 and ROBO4. Isoforms, containing the polyproline-rich regions with PPLP motifs, bind the WW domain of APBB1IP. Isoforms, containing the PPSY motif, bind, in vitro, to the WW2 and WW3 domains of NEDD4 and to the WW1 domain of YAP1. Binds the SH3 domain of BAIAP2-alpha but only after the autoinhibitory region of BAIAP2-alpha has been blocked by interaction with CDC42. Interacts, via the EVH1/WH1 domain, with the Pro-rich domains from VCL, ZYX and Listeria monocytogenes actA and with TES (via LIM domains). The TES LIM domain and the Pro-rich domains from VCL or ZYX compete for the same binding site. Interaction with ZYX is important for targeting ENAH to focal adhesions and enhances production of actin-rich structures at the apical surface of cells. Interacts, through the Pro-rich region, with the C-terminal SH3 domain of DNMPB. Binds GPHN (By similarity). Interacts with FAT1 (via EVH1 domains) (By similarity). Heterotrimer with TES and ACTL7A.
    Miscellaneous:
    • Required to transform actin polymerization into active movement for the propulsive force of Listeria monocytogenes.
    SequenceCaution:
    • Sequence=BAA91799.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAC04736.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=CAH71475.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAI22018.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAI22020.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for ENAH Gene

    Alternative splice isoforms for ENAH Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ENAH Gene

Proteomics data for ENAH Gene at MOPED

Post-translational modifications for ENAH Gene

  • NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
  • Ubiquitination at Lys69 and Lys574
  • Modification sites at PhosphoSitePlus

Other Protein References for ENAH Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for ENAH (Mena)

No data available for DME Specific Peptides for ENAH Gene

Domains for ENAH Gene

Protein Domains for ENAH Gene

Suggested Antigen Peptide Sequences for ENAH Gene

Graphical View of Domain Structure for InterPro Entry

Q8N8S7

UniProtKB/Swiss-Prot:

ENAH_HUMAN :
  • Q8N8S7
Domain:
  • The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization
  • Contains 1 WH1 domain.
Family:
  • Belongs to the Ena/VASP family.
genes like me logo Genes that share domains with ENAH: view

No data available for Gene Families for ENAH Gene

Function for ENAH Gene

Molecular function for ENAH Gene

UniProtKB/Swiss-Prot Function: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity).

Gene Ontology (GO) - Molecular Function for ENAH Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003779 actin binding IEA --
GO:0005515 protein binding IPI 16979624
GO:0017124 SH3 domain binding IEA --
GO:0050699 WW domain binding IPI 17686488
genes like me logo Genes that share ontologies with ENAH: view
genes like me logo Genes that share phenotypes with ENAH: view

Animal Model Products

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for ENAH

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for ENAH Gene

Localization for ENAH Gene

Subcellular locations from UniProtKB/Swiss-Prot for ENAH Gene

Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, filopodium. Cell junction, synapse. Cell junction, focal adhesion. Note=Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses (By similarity). {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for ENAH Gene COMPARTMENTS Subcellular localization image for ENAH gene
Compartment Confidence
cytosol 4
plasma membrane 4
cytoskeleton 2
nucleus 2

Gene Ontology (GO) - Cellular Components for ENAH Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001725 stress fiber IEA --
GO:0005737 cytoplasm IDA --
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane IDA --
GO:0005925 focal adhesion IDA --
genes like me logo Genes that share ontologies with ENAH: view

Pathways for ENAH Gene

genes like me logo Genes that share pathways with ENAH: view

Pathways by source for ENAH Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for ENAH Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001843 neural tube closure IEA --
GO:0007411 axon guidance TAS --
GO:0008154 actin polymerization or depolymerization IEA --
GO:0030036 actin cytoskeleton organization --
GO:0046907 intracellular transport IEA --
genes like me logo Genes that share ontologies with ENAH: view

Transcripts for ENAH Gene

Unigene Clusters for ENAH Gene

Enabled homolog (Drosophila):
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for ENAH

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for ENAH Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18
SP1: -
SP2:
SP3:
SP4:
SP5:
SP6:

Relevant External Links for ENAH Gene

GeneLoc Exon Structure for
ENAH
ECgene alternative splicing isoforms for
ENAH

Expression for ENAH Gene

mRNA expression in normal human tissues for ENAH Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for ENAH Gene

SOURCE GeneReport for Unigene cluster for ENAH Gene Hs.497893

mRNA Expression by UniProt/SwissProt for ENAH Gene

Q8N8S7-ENAH_HUMAN
Tissue specificity: Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
genes like me logo Genes that share expressions with ENAH: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for ENAH Gene

Orthologs for ENAH Gene

This gene was present in the common ancestor of animals.

Orthologs for ENAH Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ENAH 36
  • 69 (a)
OneToOne
cow
(Bos Taurus)
Mammalia ENAH 36
  • 66 (a)
OneToOne
dog
(Canis familiaris)
Mammalia ENAH 36
  • 65 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Enah 35
  • 88.37 (n)
  • 89.93 (a)
Enah 16
Enah 36
  • 62 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia ENAH 36
  • 59 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia ENAH 36
  • 63 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Enah 35
  • 95.07 (n)
  • 97.89 (a)
chicken
(Gallus gallus)
Aves AVENAII 36
  • 83 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ENAH 36
  • 84 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.7007 35
zebrafish
(Danio rerio)
Actinopterygii enah 36
  • 49 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta ena 36
  • 20 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea unc-34 36
  • 25 (a)
OneToMany
Species with no ortholog for ENAH:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ENAH Gene

ENSEMBL:
Gene Tree for ENAH (if available)
TreeFam:
Gene Tree for ENAH (if available)

Paralogs for ENAH Gene

Paralogs for ENAH Gene

Pseudogenes.org Pseudogenes for ENAH Gene

genes like me logo Genes that share paralogs with ENAH: view

Variants for ENAH Gene

Sequence variations from dbSNP and Humsavar for ENAH Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs6751 -- 225,494,858(-) CTACA(C/T)GCTAA utr-variant-3-prime
rs474255 -- 225,606,985(+) ACACA(A/G)ACCTG intron-variant
rs474372 -- 225,607,029(+) CGGCA(A/G)AGTGG intron-variant
rs475090 -- 225,607,039(+) GAGCA(A/G)AGTGT intron-variant
rs475600 -- 225,591,721(-) gtgac(C/G)cgatc intron-variant

Structural Variations from Database of Genomic Variants (DGV) for ENAH Gene

Variant ID Type Subtype PubMed ID
nsv832714 CNV Loss 17160897
esv34905 CNV Gain 17911159
nsv873227 CNV Gain 21882294
nsv8880 CNV Gain 18304495
nsv826842 CNV Gain 20364138
nsv441740 CNV CNV 18776908
nsv433350 CNV Gain 18776910
dgv534n71 CNV Gain 21882294
dgv185e1 CNV Complex 17122850
dgv186e1 CNV Complex 17122850
nsv4720 CNV Loss 18451855
nsv4731 CNV Insertion 18451855
esv2723872 CNV Deletion 23290073
esv2723883 CNV Deletion 23290073
esv2723894 CNV Deletion 23290073
esv2743161 CNV Deletion 23290073
esv2743163 CNV Deletion 23290073
esv2656690 CNV Deletion 23128226
nsv832725 CNV Gain 17160897
esv2743164 CNV Deletion 23290073
esv2660989 CNV Deletion 23128226
esv2723905 CNV Deletion 23290073
nsv511737 CNV Loss 21212237
esv2621711 CNV Deletion 19546169
esv2723918 CNV Deletion 23290073
esv1963994 CNV Deletion 18987734
esv4193 CNV Deletion 18987735
esv7564 CNV Loss 19470904
esv1034120 CNV Deletion 17803354
nsv832736 CNV Loss 17160897

Relevant External Links for ENAH Gene

HapMap Linkage Disequilibrium report
ENAH

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ENAH Gene

Disorders for ENAH Gene

Relevant External Links for ENAH

Genetic Association Database (GAD)
ENAH
Human Genome Epidemiology (HuGE) Navigator
ENAH
genes like me logo Genes that share disorders with ENAH: view

No data available for UniProtKB/Swiss-Prot for ENAH Gene

Publications for ENAH Gene

  1. A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family. (PMID: 9312002) Niebuhr K. … Chakraborty T. (EMBO J. 1997) 3 4 23
  2. Tes, a specific Mena interacting partner, breaks the rules for EVH1 binding. (PMID: 18158903) Boeda B. … Way M. (Mol. Cell 2007) 3 4 23
  3. Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins. (PMID: 10801818) Drees B. … Golsteyn R.M. (J. Biol. Chem. 2000) 3 4
  4. Robo4 is a vascular-specific receptor that inhibits endothelial migration. (PMID: 12941633) Park K.W. … Li D.Y. (Dev. Biol. 2003) 3 4
  5. TES is a novel focal adhesion protein with a role in cell spreading. (PMID: 12571287) Coutts A.S. … Black D.M. (J. Cell Sci. 2003) 3 23

Products for ENAH Gene

Sources for ENAH Gene

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