Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



ENAH Gene

protein-coding   GIFtS: 61
GCID: GC01M225674

Enabled Homolog (Drosophila)

  Try our

disease database 

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Enabled Homolog (Drosophila)1 2
MENA2 3 5
Mammalian Enabled1 2
ENA2 5
NDPP12 5
Protein Enabled Homolog2

External Ids:    HGNC: 182711   Entrez Gene: 557402   Ensembl: ENSG000001543807   OMIM: 6090615   UniProtKB: Q8N8S73   

Export aliases for ENAH gene to outside databases

Previous GC identifers: GC01M224165 GC01M221499 GC01M222090 GC01M222657 GC01M222659 GC01M221989 GC01M223741 GC01M196193


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

GeneCards Summary for ENAH Gene:
ENAH (enabled homolog (Drosophila)) is a protein-coding gene. GO annotations related to this gene include WW domain binding and actin binding. An important paralog of this gene is SPRED3.

UniProtKB/Swiss-Prot: ENAH_HUMAN, Q8N8S7
Function: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on
cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in
migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with
BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity)

Gene Wiki entry for ENAH Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000001.10  NT_167186.2  NC_018912.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the ENAH gene promoter:
         NF-1   NF-1/L   AML1a   NRSF form 1   NRSF form 2   LCR-F1   AREB6   HSF2   Pax-4a   ATF6   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidENAH promoter sequence
   Search Chromatin IP Primers for ENAH

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ENAH


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1q42.12   Ensembl cytogenetic band:  1q42.12   HGNC cytogenetic band: 1q32.2

ENAH Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ENAH gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M225674:  view genomic region     (about GC identifiers)

Start:
225,674,534 bp from pter      End:
225,840,845 bp from pter
Size:
166,312 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: ENAH_HUMAN, Q8N8S7 (See protein sequence)
Recommended Name: Protein enabled homolog  
Size: 591 amino acids; 66510 Da
Subunit: Homotetramer (By similarity). Interacts with APBB1IP, APBB1, PFN1 and ROBO4. Isoforms, containing the
polyproline-rich regions with PPLP motifs, bind the WW domain of APBB1IP. Isoforms, containing the PPSY motif,
bind, in vitro, to the WW2 and WW3 domains of NEDD4 and to the WW1 domain of YAP1. Binds the SH3 domain of
BAIAP2-alpha but only after the autoinhibitory region of BAIAP2-alpha has been blocked by interaction with CDC42.
Interacts, via the EVH1/WH1 domain, with the Pro-rich domains from VCL, ZYX and Listeria monocytogenes actA and
with TES (via LIM domains). The TES LIM domain and the Pro-rich domains from VCL or ZYX compete for the same
binding site. Interaction with ZYX is important for targeting ENAH to focal adhesions and enhances production of
actin-rich structures at the apical surface of cells. Interacts, through the Pro-rich region, with the C-terminal
SH3 domain of DNMPB. Binds GPHN (By similarity). Interacts with FAT1 (via EVH1 domains) (By similarity).
Heterotrimer with TES and ACTL7A
Miscellaneous: Required to transform actin polymerization into active movement for the propulsive force of
Listeria monocytogenes (By similarity)
Sequence caution: Sequence=BAA91799.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Sequence=BAC04736.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=CAH71475.1;
Type=Erroneous gene model prediction; Sequence=CAI22018.1; Type=Erroneous gene model prediction;
Sequence=CAI22020.1; Type=Erroneous gene model prediction;
3 PDB 3D structures from and Proteopedia for ENAH:
2HO2 (3D)        2IYB (3D)        2XQN (3D)    
Secondary accessions: D0PQI2 Q502W5 Q5T5M7 Q5VTQ9 Q5VTR0 Q9NVF3 Q9UFB8
Alternative splicing: 3 isoforms:  Q8N8S7-1   Q8N8S7-2   Q8N8S7-3   (Expression restricted to invasive cancer cells. Contains a phosphothreonine at position 465. Contains a phosphoserine at position 475)

Explore the universe of human proteins at neXtProt for ENAH: NX_Q8N8S7

Explore proteomics data for ENAH at MOPED

Post-translational modifications: 

  • NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions (By
    similarity)1
  • Ubiquitination2 at Lys69, Lys574
  • Modification sites at PhosphoSitePlus

  • See ENAH Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001008493.1  NP_060682.2  

    ENSEMBL proteins: 
     ENSP00000355809   ENSP00000355808   ENSP00000284563  
    Reactome Protein details: Q8N8S7

    ENAH Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Proteins for ENAH
    OriGene Protein Over-expression Lysate for ENAH
    OriGene MassSpec for ENAH
    OriGene Custom Protein Services for ENAH
    GenScript Custom Purified and Recombinant Proteins Services for ENAH
    Novus Biologicals ENAH Protein
    Novus Biologicals ENAH Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for ENAH

     
    Search eBioscience for Proteins for ENAH 

    ENAH Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of ENAH
    R&D Systems Antibodies for ENAH (Mena)
    Cell Signaling Technology (CST) Antibodies for ENAH  (Mena)
    OriGene Antibodies for ENAH
    OriGene Custom Antibody Services for ENAH
    Novus Biologicals ENAH Antibodies
    Abcam antibodies for ENAH
    Cloud-Clone Corp. Antibodies for ENAH
    ThermoFisher Antibodies for ENAH
    LSBio Antibodies in human, mouse, rat for ENAH

    ENAH Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for ENAH
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for ENAH
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for ENAH
    Cloud-Clone Corp. CLIAs for ENAH
    Search eBioscience for ELISAs for ENAH 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    4 InterPro protein domains:
     IPR000156 Ran_bind_dom
     IPR014885 VASP_tetra
     IPR011993 PH_like_dom
     IPR000697 WH1/EVH1

    Graphical View of Domain Structure for InterPro Entry Q8N8S7

    ProtoNet protein and cluster: Q8N8S7

    UniProtKB/Swiss-Prot: ENAH_HUMAN, Q8N8S7
    Domain: The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding.
    The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is
    required for F-actin binding and subcellular location, and Block C for tetramerization
    Similarity: Belongs to the Ena/VASP family
    Similarity: Contains 1 WH1 domain


    Find genes that share domains with ENAH           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ENAH_HUMAN, Q8N8S7
    Function: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on
    cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in
    migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with
    BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity)

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003779actin binding IEA--
    GO:0005515protein binding IPI16979624
    GO:0017124SH3 domain binding IEA--
    GO:0050699WW domain binding IPI17686488
         
    Find genes that share ontologies with ENAH           About GenesLikeMe


    Phenotypes:
         4 GenomeRNAi human phenotypes for ENAH:
     Decreased focal adhesion (FA)   G0/1 arrest  Increased G1 DNA content  Increased gamma-H2AX phosphory 

         8 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Enah):
     behavior/neurological  cardiovascular system  cellular  embryogenesis  growth/size/body 
     homeostasis/metabolism  mortality/aging  nervous system 

    Find genes that share phenotypes with ENAH           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ENAH
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ENAH

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for ENAH
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for ENAH

    miRNA
    Products:
        
    miRTarBase miRNAs that target ENAH:
    hsa-mir-375 (MIRT019694), hsa-mir-196a-5p (MIRT048218), hsa-mir-191-5p (MIRT045811), hsa-mir-142-3p (MIRT021597), hsa-mir-124-3p (MIRT022252), hsa-mir-181a-5p (MIRT025077), hsa-mir-21-5p (MIRT030704), hsa-mir-193b-3p (MIRT041235), hsa-mir-93-3p (MIRT038751), hsa-mir-615-3p (MIRT016152)

    Block miRNA regulation of human, mouse, rat ENAH using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ENAH (see all 237):
    hsa-miR-100* hsa-miR-26a-2* hsa-miR-376b hsa-miR-4305 hsa-miR-128 hsa-miR-1260b hsa-miR-1245 hsa-miR-138-2*
    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for ENAH
    Predesigned siRNA for gene silencing in human, mouse, rat ENAH

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for ENAH

    Clone
    Products:
         
    OriGene clones in human, mouse for ENAH (see all 11)
    OriGene ORF clones in mouse, rat for ENAH
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): ENAH (NM_018212)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ENAH
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ENAH

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for ENAH
    Browse ESI BIO Cell Lines and PureStem Progenitors for ENAH 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ENAH

    Flow Cytometry
    Products:
       

     
    eBioscience FlowRNA Probe Sets ( VA1-10879) for ENAH 


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    ENAH_HUMAN, Q8N8S7: Cytoplasm. Cytoplasm, cytoskeleton (By similarity). Cell projection, lamellipodium (By
    similarity). Cell projection, filopodium (By similarity). Cell junction, synapse (By similarity). Cell junction,
    focal adhesion. Note=Targeted to the leading edge of lamellipodia and filopodia by MRL family members.
    Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with
    N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses (By similarity)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol4
    plasma membrane4
    cytoskeleton2
    nucleus2

    Gene Ontology (GO): Selected cellular component terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001725stress fiber IEA--
    GO:0005737cytoplasm IDA--
    GO:0005829cytosol TAS--
    GO:0005886plasma membrane IDA--
    GO:0005925focal adhesion IDA--

    Find genes that share ontologies with ENAH           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for ENAH About   (see all 14)  
    See pathways by source

    SuperPathContained pathways About
    1Development Slit Robo signaling
    Development Slit Robo signaling0.92
    Cytoskeleton remodeling Slit Robo signaling0.92
    2Regulation of actin cytoskeleton
    Regulation of actin cytoskeleton0.57
    Regulation of Actin Cytoskeleton0.57
    3L1CAM interactions
    Axon guidance0.63
    Developmental Biology0.63
    4TCR signaling (REACTOME)
    TCR signaling0.73
    5Translocation of ZAP-70 to Immunological synapse
    Generation of second messenger molecules0.62


    Find genes that share SuperPaths with ENAH           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    2 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for ENAH
        Netrin Signaling
    Signaling by Slit

    2 Cell Signaling Technology (CST) Pathways for ENAH
        Neuroscience
    Cytoskeletal Signaling

    1 GeneGo (Thomson Reuters) Pathway for ENAH
        Development Slit-Robo signaling

    4 BioSystems Pathways for ENAH
        Regulation of Actin Cytoskeleton
    CDC42 signaling events
    E-cadherin signaling in the nascent adherens junction
    Stabilization and expansion of the E-cadherin adherens junction

    2 Reactome Pathways for ENAH
        Signaling by Robo receptor
    Generation of second messenger molecules


    1 Kegg Pathway  (Kegg details for ENAH):
        Regulation of actin cytoskeleton

        Pathway & Disease-focused RT2 Profiler PCR Array including ENAH: 
              Cell Motility in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for ENAH

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for ENAH (Q8N8S71, 2, 3 ENSP000003558094) via UniProtKB, MINT, STRING, and/or I2D (see all 854)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    FAT1Q145171, 2, 3, ENSP000004062294EBI-2834410,EBI-1171918 MINT-3370478 MINT-3370511 I2D: score=2 STRING: ENSP00000406229
    CHEK1O147572, 3MINT-7945693 MINT-7947479 I2D: score=3 
    APBB1IPQ7Z5R62, 3, ENSP000003654114MINT-7945693 I2D: score=1 STRING: ENSP00000365411
    SPTA1P025492, 3MINT-7211734 I2D: score=2 
    PRPF40AO754002, 3MINT-7945693 I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001843neural tube closure IEA--
    GO:0007411axon guidance TAS--
    GO:0008154actin polymerization or depolymerization IEA--
    GO:0030036actin cytoskeleton organization ----
    GO:0046907intracellular transport IEA--

    Find genes that share ontologies with ENAH           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ENAH



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for ENAH gene (2 alternative transcripts): 
    NM_001008493.1  NM_018212.4  

    Unigene Cluster for ENAH:

    Enabled homolog (Drosophila)
    Hs.497893  [show with all ESTs]
    Unigene Representative Sequence: NM_001008493
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000366844(uc001hpb.1 uc021pju.1 uc001hpc.1 uc001hpd.1)
    ENST00000488523 ENST00000358675 ENST00000366843 ENST00000284563 ENST00000498108
    ENST00000483952 ENST00000497899 ENST00000391874
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat ENAH using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ENAH (see all 237):
    hsa-miR-100* hsa-miR-26a-2* hsa-miR-376b hsa-miR-4305 hsa-miR-128 hsa-miR-1260b hsa-miR-1245 hsa-miR-138-2*
    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for ENAH
    Predesigned siRNA for gene silencing in human, mouse, rat ENAH
    Clone
    Products:
         
    OriGene clones in human, mouse for ENAH (see all 11)
    OriGene ORF clones in mouse, rat for ENAH
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): ENAH (NM_018212)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ENAH
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ENAH
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for ENAH
    OriGene qSTAR qPCR primer pairs in human, mouse for ENAH
    Pre-validated RT2 qPCR Primer Assay in human, mouse / rat ENAH
      QuantiTect SYBR Green Assays in human, mouse, rat ENAH
      QuantiFast Probe-based Assays in human, mouse, rat ENAH
    Flow Cytometry
    Products:
       

     
    eBioscience FlowRNA Probe Sets ( VA1-10879) for ENAH 

    Additional mRNA sequence: 

    AF519769.1 AK001635.1 AK025108.1 AK096246.1 AK290179.1 AL133059.1 AY345143.1 BC010414.2 
    BC037319.1 BC065238.1 BC095481.1 EU255274.1 

    23 DOTS entries:

    DT.453147  DT.99937561  DT.99963779  DT.100805979  DT.100784946  DT.91668648  DT.121348780  DT.100784952 
    DT.92441309  DT.100762854  DT.100784945  DT.121348838  DT.91896043  DT.121348775  DT.121348807  DT.121348845 
    DT.312200  DT.40209959  DT.75109500  DT.91879189  DT.95222942  DT.40267073  DT.97847181 

    Selected AceView cDNA sequences (see all 496):

    AI445758 BU172341 AA694371 AI374587 BG260305 BM978927 AF519769 CK822888 
    BF438642 AA490059 CK822887 F08624 AI494074 AA934123 BQ717087 AA927578 
    AA621893 AW304086 BM995124 BF445310 AK096246 AI089242 BU684097 BQ009946 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for ENAH (see all 6)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18
    SP1:                                                                                            -                     
    SP2:                                                                                                                  
    SP3:                                                                                                                  
    SP4:                                                                                                                  
    SP5:                                                                                                                  


    ECgene alternative splicing isoforms for ENAH

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    ENAH expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CTAACTTCGT
    ENAH Expression
    About this image


    ENAH expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 11) fully expand
     
     Brain (Nervous System)    fully expand to see all 13 entries
             Globus Pallidus
     
     Blood (Cardiovascular System)    fully expand to see all 3 entries
             Mature B-Cells Peripheral Blood
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Peripheral Nervous System (Nervous System)
             Schwann Precursor Cells Peripheral Nerve Domain
    ENAH Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ENAH Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.497893

    UniProtKB/Swiss-Prot: ENAH_HUMAN, Q8N8S7
    Tissue specificity: Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in
    colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate
    fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and
    primary breast tumor lesions

        Pathway & Disease-focused RT2 Profiler PCR Array including ENAH: 
              Cell Motility in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for ENAH
    OriGene qSTAR qPCR primer pairs in human, mouse for ENAH
    Pre-validated RT2 qPCR Primer Assay in human, mouse / rat ENAH
    QuantiTect SYBR Green Assays in human, mouse, rat ENAH
    QuantiFast Probe-based Assays in human, mouse, rat ENAH
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ENAH

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for ENAH gene from Selected species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Enah1 , 5 enabled homolog (Drosophila)1, 5 88.37(n)1
    89.93(a)1
      1 (84.93 cM)5
    138001  NM_001083120.11  NP_001076589.11 
     1819044455 
    chicken
    (Gallus gallus)
    Aves AVENAII6
    protein enabled homolog
    83(a)
    1 ↔ 1
    3(16633670-16722683)
    lizard
    (Anolis carolinensis)
    Reptilia ENAH6
    enabled homolog (Drosophila)
    84(a)
    1 ↔ 1
    1(251856306-251944297)
    African clawed frog
    (Xenopus laevis)
    Amphibia BU912927.12   -- 79.26(n)    BU912927.1 
    zebrafish
    (Danio rerio)
    Actinopterygii enah6
    enabled homolog (Drosophila)
    49(a)
    1 ↔ 1
    20(36444555-36641921) ENSDARG00000032049
    fruit fly
    (Drosophila melanogaster)
    Insecta ena6
    enabled
    20(a)
    1 → many
    2R(15029140-15050124)
    worm
    (Caenorhabditis elegans)
    Secernentea unc-346
    Protein UNC-34, isoform a
    25(a)
    1 → many
    V(982534-995404) WBGene00006770


    ENSEMBL Gene Tree for ENAH (if available)
    TreeFam Gene Tree for ENAH (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for ENAH gene
    SPRED32  SPRED12  VASP2  EVL2  SPRED22  

    Find genes that share paralogs with ENAH           About GenesLikeMe


    1 Pseudogenes.org Pseudogene for ENAH
    PGOHUM00000238301


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for ENAH (see all 2927)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1143163921,2
    F--225674240(+) AGCTCG/ATTCAT 2 -- ds50011Minor allele frequency- A:0.01WA 118
    rs175006791,2
    C,F,H--225674255(+) CAACAG/AGCTGT 2 -- ds500120Minor allele frequency- A:0.03NA NS EA 2216
    rs727530251,2
    C,F--225674295(+) GGTCAC/TCAAGC 2 -- ds50011Minor allele frequency- T:0.01NA 120
    rs1916119631,2
    --225674364(+) CCCTTA/GGTGAC 2 -- ds50010--------
    rs1855261551,2
    --225674480(+) CATGTC/TGTATA 2 -- ds50010--------
    rs1906786511,2
    --225674506(+) GAGCAA/GCTGGT 2 -- ds50010--------
    rs1814825481,2
    --225674509(+) CAACTG/TGTAAA 2 -- ds50010--------
    rs1444515701,2
    C--225674577(+) GGATAC/TCAAGA 2 -- ut310--------
    rs796255171,2
    C,F--225674654(+) TGATCC/TGATTT 2 -- ut311Minor allele frequency- T:0.02WA 118
    rs1853739121,2
    --225674670(+) ATGTGC/GCCACT 2 -- ut310--------

    HapMap Linkage Disequilibrium report for ENAH (225674534 - 225840845 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for ENAH (see all 30):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2743164CNV Deletion23290073
    esv2743161CNV Deletion23290073
    esv2723894CNV Deletion23290073
    esv2723905CNV Deletion23290073
    esv2723872CNV Deletion23290073
    esv2660989CNV Deletion23128226
    esv1034120CNV Deletion17803354
    esv1963994CNV Deletion18987734
    esv2621711CNV Deletion19546169
    esv4193CNV Deletion18987735

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ENAH
    DNA2.0 Custom Variant and Variant Library Synthesis for ENAH

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 609061    OMIM disorders: --


    Find genes that share disorders with ENAH           About GenesLikeMe

    Genetic Association Database (GAD): ENAH
    Human Genome Epidemiology (HuGE) Navigator: ENAH (4 documents)

    Export disorders for ENAH gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for ENAH gene, integrated from 10 sources (see all 79):
    (articles sorted by number of sources associating them with ENAH)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Tes, a specific Mena interacting partner, breaks the rules for EVH1 binding. (PubMed id 18158903)1, 2, 9 Boeda B.... Way M. (Mol. Cell 2007)
    2. A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family. (PubMed id 9312002)1, 2, 9 Niebuhr K.... Chakraborty T. (EMBO J. 1997)
    3. Splicing program of human MENA produces a previously undescribed isoform associated with invasive, mesenchymal-like breast tumors. (PubMed id 23129656)1, 2 Di Modugno F.... Nistico P. (Proc. Natl. Acad. Sci. U.S.A. 2012)
    4. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1, 4 Rose J.E....Uhl G.R. (Mol. Med. 2010)
    5. The enabled homolog gene polymorphisms are associated with susceptibility and progression of childhood IgA nephropathy. (PubMed id 19641378)1, 4 Hahn W.H....Kim S.D. (amp 2009)
    6. Structural basis for polyproline recognition by the FE65 WW domain. (PubMed id 17686488)1, 2 Meiyappan M.... Ladias J.A.A. (J. Mol. Biol. 2007)
    7. The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (Nature 2006)
    8. RIAM, an Ena/VASP and profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion. (PubMed id 15469846)1, 2 Lafuente E.M.... Boussiotis V.A. (Dev. Cell 2004)
    9. Human Mena protein, a serex-defined antigen overexpressed in breast cancer eliciting both humoral and CD8+ T-cell immune response. (PubMed id 15027125)1, 2 Di Modugno F.... Nistico P. (Int. J. Cancer 2004)
    10. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 55740 HGNC: 18271 AceView: ENAH Ensembl:ENSG00000154380 euGenes: HUgn55740
    ECgene: ENAH Kegg: 55740 H-InvDB: ENAH

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for ENAH Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for ENAH Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for ENAH gene:
    Search GeneIP for patents involving ENAH

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     Browse Small Molecules at EMD Millipore
     Browse Kits and Assays available from EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of ENAH
     Browse Purified and Recombinant Proteins at EMD Millipore
     
     EMD Millipore genomic analysis products

      
     Antibodies for ENAH (Mena)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for ENAH   OriGene RNAi products in human, mouse, rat for ENAH  
     OriGene qPCR primer pairs and template standards for ENAH   OriGene Protein Over-expression Lysate for ENAH  
     OriGene MassSpec something-or-other for ENAH   OriGene clones in human, mouse for ENAH  
     OriGene qSTAR qPCR primer pairs in human, mouse for ENAH   OriGene Purified Protein for ENAH  
     OriGene ORF clones in mouse, rat for ENAH   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for ENAH   OriGene Custom Protein Services for ENAH  

     
     
     Block miRNA regulation of human, mouse, rat ENAH using miScript Target Protectors SeqTarget long-range PCR primers for resequencing ENAH
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ENAH Predesigned siRNA for gene silencing in human, mouse, rat ENAH
     QuantiFast Probe-based Assays in human, mouse, rat ENAH QuantiTect SYBR Green Assays in human, mouse, rat ENAH
     PCR Arrays including human, mouse, rat ENAH Search Chromatin IP Primers for ENAH
     Pre-validated RT2 qPCR Primer Assay in human, mouse / rat ENAH  GeneGlobe Interaction Network for ENAH
     Regulatory tfbs in ENAH promoter
     GenScript Custom Purified and Recombinant Proteins Services for ENAH GenScript cDNA clones with any tag delivered in your preferred vector for ENAH
     GenScript Custom Assay Services for ENAH GenScript Custom overexpressing Cell Line Services for ENAH
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for ENAH  (Mena)

     Search Tocris compounds for ENAH
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     ENAH antibodies
     ENAH proteins
     ENAH lysates
     Antibodies for ENAH
     See all of Abcam's Antibodies, Kits and Proteins for ENAH
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for ENAH
     Antibodies for ENAH
     ELISAs for ENAH
     CLIAs for ENAH



     Browse ESI BIO Cell Lines and PureStem Progenitors for ENAH
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ENAH
     Browse SwitchGear 3'UTR luciferase reporter plasmids for ENAH
     SwitchGear Promoter luciferase reporter plasmids for ENAH
     ThermoFisher Antibodies for ENAH
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ENAH
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ENAH
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ENAH
     LSBio Antibodies in human, mouse, rat for ENAH
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
     Search Addgene for plasmids for ENAH
      Search eBioscience for proteins for ENAH
      Search eBioscience for elisas for ENAH
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      ENAH gene at Home site.
    Version: 3.12.224 13 Oct 2014
    hostname: 356980-web2.xennexinc.com index build: 128 solr: 1.4