EN2 Gene

Aliases
for EN2 gene

Previous GC identifers: GC07P153479 GC07P154552 GC07P154666 GC07P154748 GC07P154942 GC07P148981

Summaries
for EN2 gene

Genomic Views
for EN2 gene

RefSeq DNA sequence:

NC_000007.13  NC_018918.1  NT_007741.14  NT_079596.2  

SwitchGear Promoter luciferase reporter plasmids (see all 2): EN2 promoter sequence

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat EN2

EN2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)


GeneLoc information about chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07P155250:  view genomic region     (about GC identifiers)

Proteins
for EN2 gene

Explore the universe of human proteins at neXtProt for EN2: NX_P19622

Post-translational modifications:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene Protein Over-expression Lysate: EN2 OriGene Custom Protein Services for EN2
	GenScript Custom Purified and Recombinant Proteins Services for EN2
	Novus Biologicals EN2 Protein Novus Biologicals EN2 Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for EN2

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	R&D Systems Antibodies for EN2 (Engrailed-2)
	OriGene Antibodies: EN2 OriGene Custom Antibody Services for EN2
	GenScript Superior Antibodies for EN2
	Novus Biologicals EN2 Antibodies
	Search for Antibodies for EN2 at Abcam
	Uscn Antibodies for EN2
	ThermoFisher Antibodies for EN2

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for EN2
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for EN2

Protein Domains /
Families
for EN2 gene

ProtoNet protein and cluster: P19622

Function
for EN2 gene

miRNA Products:		OriGene 3'-UTR Clone: EN2 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat EN2
		8/91 QIAGEN miScript miRNA Assays for microRNAs that regulate EN2 (see all 91):
		hsa-miR-579 hsa-miR-3607-3p hsa-miR-193a-3p hsa-miR-376b hsa-miR-15a hsa-miR-128 hsa-miR-374a hsa-miR-3613-3p
		SwitchGear 3'UTR luciferase reporter plasmid: EN2 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for EN2 (see all 7) OriGene shRNA RFP: EN2 OriGene siRNA: EN2
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat EN2

Gene Editing Products:

DNA2.0 Custom Protein Engineering Service for EN2

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for EN2 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for EN2 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: EN2 (NM_001427)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for EN2
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat EN2

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for EN2
		Search LifeMap BioReagents cell lines for EN2

In Situ Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EN2

Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

(inferred from 10 alleles) (MGI details for En2)

Pathways & Interactions
for EN2 gene

Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6):    About this table

Drugs & Compounds
for EN2 gene

Transcripts
for EN2 gene

Expression
for EN2 gene

EN2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TGCATTTTAC

10/14 LifeMap In Vivo Development Anatomical Compartments/Cells (see all 14)
	Tissue Anatomical Compartment Cell			Category (developmental path)
	BrainSubstantia Nigra pars CompactaAdult Dopaminergic Neurons			Dopaminergic neurons
	BrainSubstantia Nigra pars CompactaDopaminergic Progenitor Cells			Dopaminergic neurons
	BrainSubstantia Nigra pars CompactaEmbryonic Dopaminergic Neurons			Dopaminergic neurons
	BrainVentral Tegmental AreaAdult Dopaminergic Neurons			Dopaminergic neurons
	BrainVentral Tegmental AreaDopaminergic Progenitor Cells			Dopaminergic neurons
	BrainVentral Tegmental AreaEmbryonic Dopaminergic Neurons			Dopaminergic neurons
	Neural TubeDiencephalic Floor PlateEarly Caudal Floor Plate Cells			Neural Ectoderm
	Neural TubeDiencephalic Floor PlateLate Caudal Floor Plate Cells			Neural Ectoderm
	Neural TubeDiencephalic Ventricular ZoneMeso-diencephalic Dopaminergic Precursor Cells			Dopaminergic neurons
	Neural TubeMesencephalic Floor PlateEarly Floor Plate Cells			Neural Ectoderm
Expression: Positive    Negative     Selective marker Experimental details: Curated     Microarrays     In-situ hybridization
Stem Cell Differentiation: 1 LifeMap Cell
	Name			Category
	Motor neuron-like cells (Generation of motor ...)			Motor Neurons, Spinal Cord

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for EN2 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT2 qPCR Primer Assay in human, mouse, rat EN2
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat EN2
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat EN2

In Situ Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EN2

Orthologs
for EN2 gene

Paralogs
for EN2 gene

Paralogs for EN2 gene

EN1²  VAX1²  VAX2²  

2 SIMAP similar genes for EN2 using alignment to 1 protein entry:     HME2_HUMAN:

POU6F1    EN1

EN2 for paralogs           About GeneDecksing

Genomic Variants
for EN2 gene

10/170 NCBI SNPs in EN2 are shown (see all 170)     About this table

Genomic Data					Transcription Related Data				Allele Frequencies
SNP ID	Valid	Clinical significance	Chr 7 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
-1st2nd3rd	-1st2nd3rd	-1st2nd3rd	-1st2nd3rd	--	--	--	-1st2nd3rd	--	--	-1st2nd3rd	-1st2nd3rd	-1st2nd3rd
rs734941161,2	C,	--	154482240(+)	GGCTGC/TGGCCT	1	--	us2k1		1		WA	2
rs737345211,2	C,	--	154482350(+)	AAAAAT/CGCATT	1	--	us2k1		2		WA	120
rs756084961,2	F,	--	154482547(+)	GGGCTC/TCCACC	1	--	us2k1		1		WA	118
rs559512481,2	C,F,	--	154482800(+)	ATGGAG/ACCGCA	1	--	us2k1		1		NA	120
rs557382421,2	C,	--	154482829(+)	TTTCGA/GATCGC	1	--	us2k1		0	--	--	--	--
rs563582571,2	C,F,	--	154482864(+)	GTCCCC/TGGGTG	1	--	us2k1		1		NA	120
rs37356521,2	C,F,	--	154485150(-)	GCCGGC/GGTCAG	1	--	int1		7		NA WA EA	366
rs737345231,2	C,	--	154485316(+)	CTCTTC/TCCAGC	1	--	int1		1		WA	2
rs64600131,2	C,F,A,H,	--	154485433(+)	CTCAGG/TCCAGG	1	--	int1		10		NS EA NA CSA WA	663
rs734941211,2	C,	--	154485673(+)	GCCCCC/TTTCCT	1	--	int1		2		WA	120

HapMap Linkage Disequilibrium report for EN2 (155250824 - 155257526 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for EN2
     1 CNV: 52169
Human Gene Mutation Database (HGMD): EN2

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing EN2

DNA2.0 Custom Variant and Variant Library Synthesis for EN2

Disorders / Diseases
for EN2 gene

20/22 diseases for EN2

3 Novoseek disease relationships for EN2 gene    About this table

Publications
for EN2 gene

PubMed articles for EN2 gene, integrated from 9 sources (see all 83) (see top 10): (articles sorted by number of sources associating them with EN2)

Utopia: connect your pdf to the dynamic world of online information

1. Association of the homeobox transcription factor, ENGRAILED 2, 3, with autism spectrum disorder. (PubMed id 15024396)^{1, 4, 9} Gharani N....Millonig J.H. (2004)
2. Cloning and sequence comparison of the mouse, human, and chicken engrailed genes reveal potential functional domains and regulatory regions. (PubMed id 1363401)^{1, 2, 9} Logan C....Joyner A.L. (1992)
3. Support for the homeobox transcription factor gene ENGRAILED 2 as an autism spectrum disorder susceptibility locus. (PubMed id 16252243)^{1, 2} Benayed R.... Millonig J.H. (2005)
4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
5. No association between the EN2 gene and autistic disorder. (PubMed id 12525552)^{1, 4} Zhong H....Huq A.H. (2003)
6. Human chromosome 7: DNA sequence and biology. (PubMed id 12690205)^{1, 2} Scherer S.W.... Tsui L.-C. (2003)
7. The DNA sequence of human chromosome 7. (PubMed id 12853948)^{1, 2} Hillier L.W.... Wilson R.K. (2003)
8. Isolation and chromosomal localization of the human En-2 gene. (PubMed id 2565873)^{1, 2} Poole S.J.... Lau Y.-F.C. (1989)
9. Association of the ENGRAILED 2 (EN2) gene with autism in Chinese Han population. (PubMed id 17948901)^{1, 9} Wang L....Zhang D. (2008)
10. Autism-associated haplotype affects the regulation of the homeobox gene, ENGRAILED 2. (PubMed id 19615670)^{1, 9} Benayed R....Millonig J.H. (2009)
11. Association of the homeobox transcription factor gene ENGRAILED 2 with autistic disorder in Chinese children. (PubMed id 18424904)^{1, 9} Yang P....Juo S.H. (2008)
12. Agenesis of the mesencephalon and metencephalon with cerebellar hypoplasia: putative mutation in the EN2 gene--report of 2 cases in early infancy. (PubMed id 11815869)^{1, 9} Sarnat H.B....Cheyette S.R. (2002)
13. Deregulated expression of PAX5 in medulloblastoma. (PubMed id 7777574)^{1, 9} Kozmik Z....Aguzzi A. (1995)
14. Identification of phosphorylation sites in protein kinase A substrates using artificial neural networks and mass spectrometry. (PubMed id 15253423)^{1, 9} Hjerrild M....Gammeltoft S. (2004)
15. Cut-like homeobox 1 and nuclear factor I/B mediate ENG RAILED2 autism spectrum disorder-associated haplotype function. (PubMed id 22180456)¹ Choi J....Millonig J.H. (2012)
16. The WNT2 gene polymorphism associated with speech dela y inherent to autism. (PubMed id 22522212)¹ Lin P.I....Chiu Y.N. (2012)
17. Engrailed-2 (EN2): a tumor specific urinary biomarker for the early diagnosis of prostate cancer. (PubMed id 21364037)¹ Morgan R....Pandha H. (2011)
18. Intronic single nucleotide polymorphisms of engrailed homeobox 2 modulate the disease vulnerability of autism in a han chinese popul ation. (PubMed id 20523082)¹ Yang P....Lung F.W. (2010)
19. Family-based studies indicate association of Engraile d 2 gene with autism in an Indian population. (PubMed id 20050924)¹ Sen B....Usha R. (2010)
20. Haplotype analysis of the engrailed-2 gene in young-onset Parkinson's disease. (PubMed id 19270442)¹ Rissling I....MAPller J.C. (2009)
21. Engrailed-2 regulates genes related to vesicle formation and transport in cerebellar Purkinje cells. (PubMed id 18562208)¹ Holst M.I....Baader S.L. (2008)
22. Heterogeneous association between engrailed-2 and autism in the CPEA network. (PubMed id 17948868)¹ Brune C.W....Coon H. (2008)
23. Reelin gene polymorphisms in the Indian population: a possible paternal 5'UTR-CGG-repeat-allele effect on autism. (PubMed id 16941662)¹ Dutta S....Usha R. (2007)
24. Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1. (PubMed id 17267560)¹ Sonnier L....Prochiantz A. (2007)
25. How many drug targets are there? (PubMed id 17139284)⁷ Overington J.P....Hopkins A.L. (2006)
26. Drugs, their targets and the nature and number of dru g targets. (PubMed id 17016423)⁷ Imming P....Meyer A. (2006)
27. EN2 is a candidate oncogene in human breast cancer. (PubMed id 16007149)¹ Martin N.L....Sauvageau G. (2005)
28. Joint regulation of the MAP1B promoter by HNF3beta/Foxa2 and Engrailed is the result of a highly conserved mechanism for direct interaction of homeoproteins and Fox transcription factors. (PubMed id 12642491)¹ Foucher I....Trembleau A. (2003)
29. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)¹ Strausberg R.L....Marra M.A. (2002)
30. The Protein Data Bank. (PubMed id 10592235)⁷ Berman H.M....Bourne P.E. (2000)
31. Genome-wide scan for autism susceptibility genes. Paris Autism Research International Sibpair Study. (PubMed id 10196369)¹ Philippe A....van Malldergerme L. (1999)
32. Genetic studies of autistic disorder and chromosome 7. (PubMed id 10552924)¹ Ashley-Koch A....Pericak-Vance M.A. (1999)
33. A genomic screen of autism: evidence for a multilocus etiology. (PubMed id 10417292)¹ Risch N....Myers R.M. (1999)
34. An autosomal genomic screen for autism. Collaborative linkage study of autism. (PubMed id 10581478)¹ Barrett S....Slager S. (1999)
35. Toward a complete human genome sequence. (PubMed id 9847074)¹ Wilson R. (1998)
36. A full genome screen for autism with evidence for linkage to a region on chromosome 7q. International Molecular Genetic Study of Autism Consortium. (PubMed id 9546821)¹ (1998)
37. Association of Engrailed homeoproteins with vesicles presenting caveolae-like properties. (PubMed id 9169834)¹ Joliot A....Prochiantz A. (1997)
38. Identification of homeobox genes expressed in human haemopoietic progenitor cells. (PubMed id 7518789)² Moretti P....D'Andrea R. (1994)
39. Subtle cerebellar phenotype in mice homozygous for a targeted deletion of the En-2 homeobox. (PubMed id 1672471)¹ Joyner A.L....Rossant J. (1991)
40. Chromosomal localization of the human homeo box-containing genes, EN1 and EN2. (PubMed id 2567700)¹ Logan C....Joyner A.L. (1989)
41. [EN-2: a multi-functional transcription factor of vertebrates] (PubMed id 18664177)⁹ Zhong S.C. and Yao Z.X. (2008)
42. Crossregulation between En-2 and Wnt-1 in chick tectal development. (PubMed id 9572358)⁹ Sugiyama S....Nakamura H. (1998)
43. The transcription factor Engrailed-2 guides retinal axons. (PubMed id 16267555)⁹ Brunet I....Holt C. (2005)
44. Involvement of the EN-2 gene in normal and abnormal development of the human arcuate nucleus. (PubMed id 15676030)⁹ Lavezzi A.M....Matturri L. (2005)
45. Protein kinase A phosphorylates serine 267 in the homeodomain of engrailed-2 leading to decreased DNA binding. (PubMed id 15196920)⁹ Hjerrild M....Rasmussen T.E. (2004)
46. Further observations on the susceptibility of diencephalic prosomeres to En-2 induction and on the resulting histogenetic capabilities. (PubMed id 8887316)⁹ Bloch-Gallego E....Alvarado-Mallart R.M. (1996)
47. Dorsoventral patterning of the avian mesencephalon/metencephalon: role of the notochord and floor plate in suppressing Engrailed-2. (PubMed id 7714526)⁹ Darnell D.K. and Schoenwolf G.C. (1995)
48. Expression of the homeobox-containing gene En-2 during the development of the chick central nervous system. (PubMed id 7620626)⁹ Millet S. and Alvarado-Mallart R.M. (1995)
49. Increased susceptibility to kainic acid-induced seizures in Engrailed-2 knockout mice. (PubMed id 19186208)⁹ Tripathi P.P....Bozzi Y. (2009)
50. En-2 regulates the expression of the ligands for Eph type tyrosine kinases in chick embryonic tectum. (PubMed id 9129179)⁹ Shigetani Y....Nakamura H. (1997)
51. Gene silencing in chick embryos with a vector-based small interfering RNA system. (PubMed id 12950277)⁹ Katahira T. and Nakamura H. (2003)
52. Vertical regulation of En-2 expression and eye development by FGFs and BMPs. (PubMed id 11100736)⁹ Mayordomo R. and Alvarez I.S. (2000)
53. Expression of the homeobox-containing genes EN1 and EN2 in human fetal midgestational medulla and cerebellum. (PubMed id 9056537)⁹ Zec N....Kinney H.C. (1997)
54. Retroviral misexpression of engrailed genes in the chick optic tectum perturbs the topographic targeting of retinal axons. (PubMed id 8757262)⁹ Friedman G.C. and O'Leary D.D. (1996)
55. Cephalic expression and molecular characterization of Xenopus En-2. (PubMed id 1679005)⁹ Hemmati-Brivanlou A....Harland R.M. (1991)
56. Cerebellum development and schizophrenia: an association study of the human homeogene Engrailed 2. (PubMed id 15123388)⁹ Gourion D....Krebs M.O. (2004)
57. Search for genes involved in Joubert syndrome: evidence that one or more major loci are yet to be identified and exclusion of candidate genes EN1, EN2, FGF8, and BARHL1. (PubMed id 11807898)⁹ Blair I.P....Chance P.F. (2002)
58. Early patterning of the prospective midbrain-hindbrain boundary by the HES-related gene XHR1 in Xenopus embryos. (PubMed id 11731236)⁹ Shinga J....Taira M. (2001)
59. Evidence that FGF8 signalling from the midbrain-hindbrain junction regulates growth and polarity in the developing midbrain. (PubMed id 9056772)⁹ Lee S.M....McMahon A.P. (1997)
60. Ventral ectoderm of Xenopus forms neural tissue, including hindbrain, in response to activin. (PubMed id 1425347)⁹ Bolce M.E....Harland R.M. (1992)
61. Expression patterns of engrailed-like proteins in the chick embryo. (PubMed id 1354990)⁹ Gardner C.A. and Barald K.F. (1992)
62. Region-specific neural induction of an engrailed protein by anterior notochord in Xenopus. (PubMed id 1978411)⁹ Hemmati-Brivanlou A....Harland R.M. (1990)
63. Polymorphisms of coding trinucleotide repeats of homeogenes in neurodevelopmental psychiatric disorders. (PubMed id 19018235)⁹ Laroche F....Robel L. (2008)
64. En2 knockout mice display neurobehavioral and neurochemical alterations relevant to autism spectrum disorder. (PubMed id 16935268)⁹ Cheh M.A....Wagner G.C. (2006)
65. Three autism candidate genes: a synthesis of human genetic analysis with other disciplines. (PubMed id 15749247)⁹ Bartlett C.W....Brzustowicz L.M. (2005)
66. Signaling specificities of fibroblast growth factor receptors in early Xenopus embryo. (PubMed id 10910771)⁹ Umbhauer M....Riou J. (2000)
67. Antagonizing activity of chick Grg4 against tectum-organizing activity. (PubMed id 10772799)⁹ Sugiyama S....Nakamura H. (2000)
68. Selective disruption of 'late onset' sagittal banding patterns by ectopic expression of engrailed-2 in cerebellar Purkinje cells. (PubMed id 10377347)⁹ Baader S.L....Oberdick J. (1999)
69. Roles of Pax-2 in initiation of the chick tectal development. (PubMed id 10446345)⁹ Okafuji T....Nakamura H. (1999)
70. Misexpression of Polycomb-group proteins in Xenopus alters anterior neural development and represses neural target genes. (PubMed id 10545244)⁹ Yoshitake Y....Hollenberg S.M. (1999)
71. Ectopic overexpression of engrailed-2 in cerebellar Purkinje cells causes restricted cell loss and retarded external germinal layer development at lobule junctions. (PubMed id 9465001)⁹ Baader S.L....Oberdick J. (1998)
72. The chick/quail chimeric system: a model for early ce rebellar development. (PubMed id 9509515)⁹ Hallonet M. and Alvarado-Mallart R.M. (1997)
73. Fibroblast growth factor is a direct neural inducer, which combined with noggin generates anterior-posterior neural pattern. (PubMed id 8582276)⁹ Lamb T.M. and Harland R.M. (1995)
74. Ectopic induction and reorganization of Wnt-1 expression in quail/chick chimeras. (PubMed id 7821210)⁹ Bally-Cuif L. and Wassef M. (1994)
75. 9-Methyl-beta-carboline up-regulates the appearance of differentiated dopaminergic neurones in primary mesencephalic culture. (PubMed id 17913302)⁹ Hamann J....Gille G. (2008)
76. In vitro differentiation of cord blood unrestricted somatic stem cells expressing dopamine-associated genes into neuron-like cells. (PubMed id 17196845)⁹ Fallahi-Sichani M....Atashi A. (2007)
77. Deletions of 2q14 that include the homeobox engrailed 1 (EN1) transcription factor are compatible with a normal phenotype. (PubMed id 16552425)⁹ Barber J.C....Wakeling E. (2006)
78. The zic1 gene is an activator of Wnt signaling. (PubMed id 16892174)⁹ Merzdorf C.S. and Sive H.L. (2006)
79. Postnatal cerebellar defects in mice deficient in methylenetetrahydrofolate reductase. (PubMed id 15979267)⁹ Chen Z....Rozen R. (2005)
80. Regionalisation and acquisition of polarity in the optic tectum. (PubMed id 11689282)⁹ Nakamura H. (2001)
81. foxD5a, a Xenopus winged helix gene, maintains an immature neural ectoderm via transcriptional repression that is dependent on the C-terminal domain. (PubMed id 11401404)⁹ Sullivan S.A....Moody S.A. (2001)

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