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Aliases for EN2 Gene

Aliases for EN2 Gene

  • Engrailed Homeobox 2 2 3
  • Homeobox Protein En-2 3 4
  • Hu-En-2 3 4
  • Engrailed Homolog 2 3
  • Engrailed-2 3

External Ids for EN2 Gene

Previous GeneCards Identifiers for EN2 Gene

  • GC07P153479
  • GC07P154552
  • GC07P154666
  • GC07P154748
  • GC07P154942
  • GC07P155250
  • GC07P148981

Summaries for EN2 Gene

Entrez Gene Summary for EN2 Gene

  • Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]

GeneCards Summary for EN2 Gene

EN2 (Engrailed Homeobox 2) is a Protein Coding gene. Diseases associated with EN2 include young-onset parkinson disease and autistic disorder. GO annotations related to this gene include sequence-specific DNA binding. An important paralog of this gene is EMX2.

Gene Wiki entry for EN2 Gene

No data available for UniProtKB/Swiss-Prot , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for EN2 Gene

Genomics for EN2 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for EN2 Gene

155,458,129 bp from pter
155,464,831 bp from pter
6,703 bases
Plus strand

Genomic View for EN2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for EN2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for EN2 Gene

Proteins for EN2 Gene

  • Protein details for EN2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Homeobox protein engrailed-2
    Protein Accession:
    Secondary Accessions:
    • A4D252
    • Q549U3
    • Q9UD58

    Protein attributes for EN2 Gene

    333 amino acids
    Molecular mass:
    34211 Da
    Quaternary structure:
    No Data Available

neXtProt entry for EN2 Gene

Proteomics data for EN2 Gene at MOPED

Post-translational modifications for EN2 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for EN2 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

No data available for DME Specific Peptides for EN2 Gene

Domains & Families for EN2 Gene

Gene Families for EN2 Gene

Suggested Antigen Peptide Sequences for EN2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Contains 1 homeobox DNA-binding domain.
  • Belongs to the engrailed homeobox family.
  • Contains 1 homeobox DNA-binding domain.
  • Belongs to the engrailed homeobox family.
genes like me logo Genes that share domains with EN2: view

Function for EN2 Gene

Molecular function for EN2 Gene

GENATLAS Biochemistry:
Drosophila segment-polarity gene engrailed homolog 2,homeo domain encoding gene

Gene Ontology (GO) - Molecular Function for EN2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding --
GO:0043565 sequence-specific DNA binding IEA --
genes like me logo Genes that share ontologies with EN2: view
genes like me logo Genes that share phenotypes with EN2: view

Animal Models for EN2 Gene

MGI Knock Outs for EN2:

miRNA for EN2 Gene

miRTarBase miRNAs that target EN2

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for EN2

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targets and HOMER Transcription for EN2 Gene

Localization for EN2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for EN2 Gene

Subcellular locations from

Jensen Localization Image for EN2 Gene COMPARTMENTS Subcellular localization image for EN2 gene
Compartment Confidence
nucleus 5
extracellular 1

Gene Ontology (GO) - Cellular Components for EN2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0016020 membrane IEA --
genes like me logo Genes that share ontologies with EN2: view

Pathways & Interactions for EN2 Gene

SuperPathways for EN2 Gene

No Data Available

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for EN2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006355 regulation of transcription, DNA-templated --
GO:0007275 multicellular organismal development TAS 1672471
GO:0030182 neuron differentiation --
GO:0030901 midbrain development IEA --
GO:0030902 hindbrain development IEA --
genes like me logo Genes that share ontologies with EN2: view

No data available for Pathways by source and SIGNOR curated interactions for EN2 Gene

Drugs & Compounds for EN2 Gene

(2) Drugs for EN2 Gene - From: DrugBank and NovoSeek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
N-Cyclohexyltaurine Experimental Pharma Target 0
tyrosine Nutra 547
genes like me logo Genes that share compounds with EN2: view

Transcripts for EN2 Gene

mRNA/cDNA for EN2 Gene

(1) REFSEQ mRNAs :
(2) Additional mRNA sequences :
(10) Selected AceView cDNA sequences:
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for EN2 Gene

Engrailed homeobox 2:
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for EN2

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for EN2 Gene

No ASD Table

Relevant External Links for EN2 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for EN2 Gene

mRNA expression in normal human tissues for EN2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for EN2 Gene

This gene is overexpressed in Brain - Cerebellar Hemisphere (x23.4) and Brain - Cerebellum (x22.0).

Protein differential expression in normal tissues from HIPED for EN2 Gene

This gene is overexpressed in Pancreatic juice (54.4) and Adipocyte (14.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for EN2 Gene

SOURCE GeneReport for Unigene cluster for EN2 Gene Hs.134989

genes like me logo Genes that share expression patterns with EN2: view

Protein tissue co-expression partners for EN2 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for EN2 Gene

Orthologs for EN2 Gene

This gene was present in the common ancestor of animals.

Orthologs for EN2 Gene

Organism Taxonomy Gene Similarity Type Details
(Mus musculus)
Mammalia En2 35
  • 84.47 (n)
  • 86.73 (a)
En2 16
En2 36
  • 89 (a)
(Pan troglodytes)
Mammalia EN2 35
  • 98.7 (n)
  • 99.1 (a)
EN2 36
  • 99 (a)
(Rattus norvegicus)
Mammalia En2 35
  • 84.73 (n)
  • 86.07 (a)
(Bos Taurus)
Mammalia EN2 36
  • 87 (a)
(Canis familiaris)
Mammalia EN2 36
  • 79 (a)
(Monodelphis domestica)
Mammalia EN2 36
  • 76 (a)
(Ornithorhynchus anatinus)
Mammalia EN2 36
  • 84 (a)
(Gallus gallus)
Aves LOC100859435 35
  • 76.28 (n)
  • 77.69 (a)
EN2 36
  • 76 (a)
(Anolis carolinensis)
Reptilia EN2 36
  • 67 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100498251 35
  • 66.52 (n)
  • 73.08 (a)
(Danio rerio)
Actinopterygii eng2a 35
eng2b 35
  • 62.18 (n)
  • 68.49 (a)
eng2a 36
  • 65 (a)
eng2b 36
  • 67 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.6099 35
fruit fly
(Drosophila melanogaster)
Insecta en 37
  • 74 (a)
inv 37
  • 62 (a)
CG34031 36
  • 22 (a)
en 36
  • 22 (a)
inv 36
  • 23 (a)
lms 36
  • 17 (a)
ro 36
  • 19 (a)
(Caenorhabditis elegans)
Secernentea ceh-16 36
  • 43 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.4370 36
  • 29 (a)
Species with no ortholog for EN2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for EN2 Gene

Gene Tree for EN2 (if available)
Gene Tree for EN2 (if available)

Paralogs for EN2 Gene

Paralogs for EN2 Gene

(2) SIMAP similar genes for EN2 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with EN2: view

Variants for EN2 Gene

Sequence variations from dbSNP and Humsavar for EN2 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type MAF
rs1345514 -- 155,456,455(+) AACAA(C/T)GGGTG upstream-variant-2KB
rs1861958 -- 155,465,199(+) GGGAC(A/C/G)GGAAC downstream-variant-500B
rs1861972 Uncertain significance, Uncertain significance 155,461,298(+) TGCCA(A/G)TGGCC intron-variant
rs1861973 Uncertain significance, Uncertain significance 155,461,450(+) CCCTG(C/T)CCAAA intron-variant
rs1895091 -- 155,464,862(+) GTTTT(A/C)TAAGC downstream-variant-500B

Structural Variations from Database of Genomic Variants (DGV) for EN2 Gene

Variant ID Type Subtype PubMed ID
nsv824408 CNV Gain 20364138
nsv831196 CNV Gain 17160897
nsv824413 CNV Gain 20364138
nsv520626 CNV Loss 19592680

Variation tolerance for EN2 Gene

Gene Damage Index Score: 7.14; 80.67% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for EN2 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for EN2 Gene

Disorders for EN2 Gene

MalaCards: The human disease database

(3) MalaCards diseases for EN2 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
young-onset parkinson disease
  • early-onset parkinson disease
autistic disorder
  • autism
autism spectrum disorder
  • autism spectrum disorders
- elite association
Search EN2 in MalaCards View complete list of genes associated with diseases


  • Note=Genetic variations in EN2 may be associated with susceptibility to autism.

Relevant External Links for EN2

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
genes like me logo Genes that share disorders with EN2: view

No data available for Genatlas for EN2 Gene

Publications for EN2 Gene

  1. Autism-associated haplotype affects the regulation of the homeobox gene, ENGRAILED 2. (PMID: 19615670) Benayed R. … Millonig J.H. (Biol. Psychiatry 2009) 23 67
  2. Increased susceptibility to kainic acid-induced seizures in Engrailed-2 knockout mice. (PMID: 19186208) Tripathi P.P. … Bozzi Y. (Neuroscience 2009) 23 67
  3. 9-Methyl-beta-carboline up-regulates the appearance of differentiated dopaminergic neurones in primary mesencephalic culture. (PMID: 17913302) Hamann J. … Gille G. (Neurochem. Int. 2008) 23 67
  4. Association of the ENGRAILED 2 (EN2) gene with autism in Chinese Han population. (PMID: 17948901) Wang L. … Zhang D. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2008) 23 67
  5. Association of the homeobox transcription factor gene ENGRAILED 2 with autistic disorder in Chinese children. (PMID: 18424904) Yang P. … Juo S.H. (Neuropsychobiology 2008) 23 67

Products for EN2 Gene

Sources for EN2 Gene

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